Expression of target protein and Affibody molecule in Pichia pastoris
... The target protein EpCAM (Epidermal Cell Adhesion Molecule), also known as 17-1A antigen, is a human cancer antigen displayed in excess on the tumour cell surface of many common cancers such as colon, lung and breast cancers (Balzar et al., 1999). A specific binder towards the antigen would be able ...
... The target protein EpCAM (Epidermal Cell Adhesion Molecule), also known as 17-1A antigen, is a human cancer antigen displayed in excess on the tumour cell surface of many common cancers such as colon, lung and breast cancers (Balzar et al., 1999). A specific binder towards the antigen would be able ...
- Wiley Online Library
... Rab23 sequence is the exceptional length of the C-terminal tail, which is significantly longer than that of other Rab members. This hypervariable C-terminal domain of Rab proteins is thought to be a targeting signal crucial for specific membrane association (Chavrier et al., 1991). Sequence comparison ...
... Rab23 sequence is the exceptional length of the C-terminal tail, which is significantly longer than that of other Rab members. This hypervariable C-terminal domain of Rab proteins is thought to be a targeting signal crucial for specific membrane association (Chavrier et al., 1991). Sequence comparison ...
Chapter 3 PowerPoint - Hillsborough Community College
... • Receptor-mediated endocytosis involves endocytosis and transcytosis of specific molecules – Many cells have receptors embedded in clathrin-coated pits, which will be internalized along with the specific molecule bound • Examples: enzymes, low-density lipoproteins (LDL), iron, insulin, and, unfortu ...
... • Receptor-mediated endocytosis involves endocytosis and transcytosis of specific molecules – Many cells have receptors embedded in clathrin-coated pits, which will be internalized along with the specific molecule bound • Examples: enzymes, low-density lipoproteins (LDL), iron, insulin, and, unfortu ...
Identification of Hedgehog Pathway Components by RNAi in
... Classical genetic screens can be limited by the selectivity of mutational targeting, the complexities of anatomically based phenotypic analysis, or difficulties in subsequent gene identification. Focusing on signaling response to the secreted morphogen Hedgehog (Hh), we used RNA interference (RNAi) an ...
... Classical genetic screens can be limited by the selectivity of mutational targeting, the complexities of anatomically based phenotypic analysis, or difficulties in subsequent gene identification. Focusing on signaling response to the secreted morphogen Hedgehog (Hh), we used RNA interference (RNAi) an ...
Geranylgeranylated Proteins are Involved in the Regulation of
... RhoA have been implicated in the regulation of cell cycle progression through G1 phase of the cell cycle. Constitutively activated mutants of Rac-1, Cdc42, and RhoA caused G1 progression and stimulation of DNA synthesis in fibroblasts (20 – 22). Furthermore, expression of active forms of Rac-1, Cdc4 ...
... RhoA have been implicated in the regulation of cell cycle progression through G1 phase of the cell cycle. Constitutively activated mutants of Rac-1, Cdc42, and RhoA caused G1 progression and stimulation of DNA synthesis in fibroblasts (20 – 22). Furthermore, expression of active forms of Rac-1, Cdc4 ...
Receptor Fragments: Intracellular Signaling and
... either as holoproteins or protein cleavage fragments (Fig. 1). In fact, the most innovative and exciting studies at the receptor forefront converge on the idea that many conventional plasma membrane receptors also accumulate within cell nuclei (within the nuclear membrane and/or nucleosol) and that ...
... either as holoproteins or protein cleavage fragments (Fig. 1). In fact, the most innovative and exciting studies at the receptor forefront converge on the idea that many conventional plasma membrane receptors also accumulate within cell nuclei (within the nuclear membrane and/or nucleosol) and that ...
2) A dynamic cell adhesion surface regulates tissue architecture in
... surface enriched in cadherins and β-catenin. Rearrangement into columns occurs concomitant with expansion of this adhesion surface in a process more similar to cell spreading than to migration. Column formation requires cell-cell adhesion, as reducing cadherin binding via chelation of extracellular ...
... surface enriched in cadherins and β-catenin. Rearrangement into columns occurs concomitant with expansion of this adhesion surface in a process more similar to cell spreading than to migration. Column formation requires cell-cell adhesion, as reducing cadherin binding via chelation of extracellular ...
Plant responses to environmental stress
... the TCH genes (Braam 1992). Treatment with Mg2+ under similar conditions has no eect on TCH gene expression (Braam 1992). These data are consistent with the possibility that changes in [Ca2+] are sucient for TCH gene upregulation of expression. However, it has not been ruled out that increased ext ...
... the TCH genes (Braam 1992). Treatment with Mg2+ under similar conditions has no eect on TCH gene expression (Braam 1992). These data are consistent with the possibility that changes in [Ca2+] are sucient for TCH gene upregulation of expression. However, it has not been ruled out that increased ext ...
Organelle communication - Repositorio Académico
... The nuclear envelope (NE), which is continuous with the ER, can also communicate with the PM, leading to direct signaling from the extracellular space into the nucleus. The mechanism used by the NE also relies on Ca2+ signaling, and is able to trigger nuclear Ca2+ transients independently of the cyt ...
... The nuclear envelope (NE), which is continuous with the ER, can also communicate with the PM, leading to direct signaling from the extracellular space into the nucleus. The mechanism used by the NE also relies on Ca2+ signaling, and is able to trigger nuclear Ca2+ transients independently of the cyt ...
senescent cells
... A common feature of aging tissues is low-level chronic inflammation, termed sterile inflammation (indicating an absence of detectable pathogens) or inflammaging (69–72). Chronic inflammation can drive pathology by at least two mechanisms. First, infiltrating immune cells can degrade tissues because ...
... A common feature of aging tissues is low-level chronic inflammation, termed sterile inflammation (indicating an absence of detectable pathogens) or inflammaging (69–72). Chronic inflammation can drive pathology by at least two mechanisms. First, infiltrating immune cells can degrade tissues because ...
to the complete text
... threefold [15]. Mutation of RasG also suggests an important role of the Ras family for the regulation of the actin cytoskeleton. Dicytostelium with rasG mutations show normal growth but aberrant actin structures and defects in cytokinesis [16] — presence of F-actin deposits and lack of cell polarity ...
... threefold [15]. Mutation of RasG also suggests an important role of the Ras family for the regulation of the actin cytoskeleton. Dicytostelium with rasG mutations show normal growth but aberrant actin structures and defects in cytokinesis [16] — presence of F-actin deposits and lack of cell polarity ...
Introduction to Carbohydrates
... IV. Degradation of glycosaminoglycans - GAGs are degraded in lysosomes, which contain hydrolytic enz’s that are most active at a pH of ~ 5 [Note: therefore, as a group, these are called acid hydrolases] - The low pH optimum is a protective mechanism that prevents the enz’s from destroying the cell ...
... IV. Degradation of glycosaminoglycans - GAGs are degraded in lysosomes, which contain hydrolytic enz’s that are most active at a pH of ~ 5 [Note: therefore, as a group, these are called acid hydrolases] - The low pH optimum is a protective mechanism that prevents the enz’s from destroying the cell ...
Glycosylation of Antibodies - Department of Chemistry
... An antibody is a protein of the immune system which is made up of four polypeptides, two light chains and two heavy chains.3 Antibodies are also in the shape of a “Y” with the antigen binding sites at the two ends, named the variable region (Fab), and the stem, known as the constant region(Fc). ...
... An antibody is a protein of the immune system which is made up of four polypeptides, two light chains and two heavy chains.3 Antibodies are also in the shape of a “Y” with the antigen binding sites at the two ends, named the variable region (Fab), and the stem, known as the constant region(Fc). ...
Metabolic Incorporation of Stable Isotope Labels into Glycans
... units from glycans grown in the 14N and amide -15N, respectively (Figure 2C and D). In these spectra, the most intense ion appears at the monoisotopic mass calculated with either 14N or two 15N, demonstrating that the majority of this glycan has 15N incorporated into both possible sites. Ions are pr ...
... units from glycans grown in the 14N and amide -15N, respectively (Figure 2C and D). In these spectra, the most intense ion appears at the monoisotopic mass calculated with either 14N or two 15N, demonstrating that the majority of this glycan has 15N incorporated into both possible sites. Ions are pr ...
The Role of the Leukemia Inhibitory Factor (LIF)
... the LIF/STAT3-pathway in vivo. However, studies on knockout mouse models indicate that at least the early embryonic development can occur also in the absence of some components of the LIF-pathway but many of these mouse models then display later on during development or during adulthood many differe ...
... the LIF/STAT3-pathway in vivo. However, studies on knockout mouse models indicate that at least the early embryonic development can occur also in the absence of some components of the LIF-pathway but many of these mouse models then display later on during development or during adulthood many differe ...
Critical regulation of CD4 T cell survival and autoimmunity by b
... first 2 d after CD4+ T cell activation, b-arrestin 1 and Bcl-2 protein gradually decreased but then mostly recovered by day 3 and remained high thereafter (Fig. 2a). To analyze the changes of Arrb1 and Bcl2 expression in more detail, we collected samples every 4 h from 40 h to 64 h after CD4+ T cell ...
... first 2 d after CD4+ T cell activation, b-arrestin 1 and Bcl-2 protein gradually decreased but then mostly recovered by day 3 and remained high thereafter (Fig. 2a). To analyze the changes of Arrb1 and Bcl2 expression in more detail, we collected samples every 4 h from 40 h to 64 h after CD4+ T cell ...
Oligomerization of Epidermal Growth Factor Receptors on A431
... obvious gaps in the mechanism. However, the initial steps in EGFR activation (for reviews see references 40, 43, 50) remain controversial. Two models have been proposed. The first stipulates that external binding of the growth hormone stimulates the tyrosine kinase activity in the cytoplasmic domain ...
... obvious gaps in the mechanism. However, the initial steps in EGFR activation (for reviews see references 40, 43, 50) remain controversial. Two models have been proposed. The first stipulates that external binding of the growth hormone stimulates the tyrosine kinase activity in the cytoplasmic domain ...
Events at the Host-Microbial Interface of the Gastrointestinal Tract III
... consistent with previous observations indicating that AI-2 fractions of Salmonella and E. coli only yielded several furanosyl compounds that did not contain boron (12, 21, 24). Diverse roles in signaling have been attributed to AI-2 in other organisms by comparing luxS mutants with wild-type strains ...
... consistent with previous observations indicating that AI-2 fractions of Salmonella and E. coli only yielded several furanosyl compounds that did not contain boron (12, 21, 24). Diverse roles in signaling have been attributed to AI-2 in other organisms by comparing luxS mutants with wild-type strains ...
Bone Marrow Norepinephrine Release in the Spleen and Cells and
... essential for maintaining an optimal Th2 cell-dependent Ab response in vivo. However, for NE to influence immune cell function, it must be released at the immediate site of action, since it is either rapidly degraded by catechol-O-methyltransferase and monoamine oxidase, diffused into the circulatio ...
... essential for maintaining an optimal Th2 cell-dependent Ab response in vivo. However, for NE to influence immune cell function, it must be released at the immediate site of action, since it is either rapidly degraded by catechol-O-methyltransferase and monoamine oxidase, diffused into the circulatio ...
Cell size: a consequence of growth and division?
... amount of cell division that occurs during imaginal disc development. Wings from flies that completely lack chico possess fewer cells than do wild-type wings (Bohni et al., 1999). Similarly, overexpression of Dp110 in a large area and during an extended period of wing development* can result in a mi ...
... amount of cell division that occurs during imaginal disc development. Wings from flies that completely lack chico possess fewer cells than do wild-type wings (Bohni et al., 1999). Similarly, overexpression of Dp110 in a large area and during an extended period of wing development* can result in a mi ...
Interaction of Lead and Calcium with Lead
... Calcium was inhibitory to each organism tested. Lead was not able to reverse the inhibition of E. coli Ca, therefore they probably are not competing for similar cell sites. Calcium exacerbated inhibition of Pb4 by Lead suggesting that their mode of action may be similar. Calcium did reverse ...
... Calcium was inhibitory to each organism tested. Lead was not able to reverse the inhibition of E. coli Ca, therefore they probably are not competing for similar cell sites. Calcium exacerbated inhibition of Pb4 by Lead suggesting that their mode of action may be similar. Calcium did reverse ...