Caveolin-3 and SAP97 form a scaffolding protein complex that
... shown). Therefore, the Cav-3 that was pulled down from heart extracts by GST1.5C1 likely represents the fraction bound to SAP97. The interaction between SAP97 and Cav-3 was confirmed in cotransfection experiments and in vitro. Figure 1C shows that anti-PDZ antibody specifically coprecipitated Cav-3 ...
... shown). Therefore, the Cav-3 that was pulled down from heart extracts by GST1.5C1 likely represents the fraction bound to SAP97. The interaction between SAP97 and Cav-3 was confirmed in cotransfection experiments and in vitro. Figure 1C shows that anti-PDZ antibody specifically coprecipitated Cav-3 ...
Hornstein, E. - Weizmann Institute of Science
... thousands of predicted targets on the other hand, is intriguing. One possible interpretation is that miRNAs may mainly play accessory role, being involved in enhancing the fidelity of primary gene expression programs. ...
... thousands of predicted targets on the other hand, is intriguing. One possible interpretation is that miRNAs may mainly play accessory role, being involved in enhancing the fidelity of primary gene expression programs. ...
A Model-Based Approach for Automated In Vitro Cell - LISA
... Preprocessing. We applied image preprocessing steps to avoid illumination problems (such as vignetting and illumination changes) during the experiment and to enhance image contrast. These steps, which were applied to the complete image sequence, essentially included image background detection, backg ...
... Preprocessing. We applied image preprocessing steps to avoid illumination problems (such as vignetting and illumination changes) during the experiment and to enhance image contrast. These steps, which were applied to the complete image sequence, essentially included image background detection, backg ...
review - Microbiology
... function of this complex locus (Anderson, 1979; Anderson e t al., 1989). As in sexual development, apogamic amoebae undergo a period of proliferation before clonal plasmodium formation is initiated (Youngman e t al., 1977). The proliferating amoebae secrete a chemical inducer and plasmodium formatio ...
... function of this complex locus (Anderson, 1979; Anderson e t al., 1989). As in sexual development, apogamic amoebae undergo a period of proliferation before clonal plasmodium formation is initiated (Youngman e t al., 1977). The proliferating amoebae secrete a chemical inducer and plasmodium formatio ...
Chemical approaches to study metabolic networks
... superfamily, one of the largest and most diverse metabolic enzyme classes in mammalian proteomes, which include esterases, thioesterases, lipases, amidases, and proteases [41]. The fluorophosphonate ABPs for the serine hydrolase superfamily has been used to discover several dysregulated enzymes in d ...
... superfamily, one of the largest and most diverse metabolic enzyme classes in mammalian proteomes, which include esterases, thioesterases, lipases, amidases, and proteases [41]. The fluorophosphonate ABPs for the serine hydrolase superfamily has been used to discover several dysregulated enzymes in d ...
Glucocorticoids Regulate Pituitary Growth Hormone
... GH pathway (1, 12). Previously, it was demonstrated that GHRH-binding sites were down-regulated in dispersed pituitary cells from adrenalectomized rats, and hormone replacement with the synthetic glucocorticoid, dexamethasone, was able to restore GHRH binding (16, 17). Significantly, these changes i ...
... GH pathway (1, 12). Previously, it was demonstrated that GHRH-binding sites were down-regulated in dispersed pituitary cells from adrenalectomized rats, and hormone replacement with the synthetic glucocorticoid, dexamethasone, was able to restore GHRH binding (16, 17). Significantly, these changes i ...
Intercellular signalling and the multiplication of prokaryotes
... 1976) is that a bacterial cell should only be considered as viable if it is capable of multiplying (Barer, 1997; Barer et al., 1993; Kell et al., 1998). In particular, it is necessary to consider that terms such as ‘viability’ can have both a conceptual meaning, in which we ascribe a state of say ...
... 1976) is that a bacterial cell should only be considered as viable if it is capable of multiplying (Barer, 1997; Barer et al., 1993; Kell et al., 1998). In particular, it is necessary to consider that terms such as ‘viability’ can have both a conceptual meaning, in which we ascribe a state of say ...
Supplementary Methods and References
... preparation led to a ~7 mM MnO2 emulsion. TMD NPs were stored at 4 °C and further diluted with cell medium or sterile saline for in vitro and in vivo studies, respectively. Preparation of LMD NPs: In a 14 mL polypropylene conical tube, a mixture of myristic acid (7 mg), polyoxyethylene (40) stearate ...
... preparation led to a ~7 mM MnO2 emulsion. TMD NPs were stored at 4 °C and further diluted with cell medium or sterile saline for in vitro and in vivo studies, respectively. Preparation of LMD NPs: In a 14 mL polypropylene conical tube, a mixture of myristic acid (7 mg), polyoxyethylene (40) stearate ...
Document
... They may make their own growth factor They may convey a growth factor’s signal without the presence of the growth factor ...
... They may make their own growth factor They may convey a growth factor’s signal without the presence of the growth factor ...
Modulation of Retinoblastoma and Retinoblastoma
... wild-type cell proliferates normally (28). The two populations of cells may represent the difference in cell function ...
... wild-type cell proliferates normally (28). The two populations of cells may represent the difference in cell function ...
Regulation of multiple target genes by miR-1 and miR
... miR-206 family is expressed during skeletal muscle differentiation and is an integral component of myogenesis. To better understand miR-1/miR-206 function during myoblast differentiation we identified novel target mRNAs by microarray and characterized their function in C2C12 myoblasts. Candidate tar ...
... miR-206 family is expressed during skeletal muscle differentiation and is an integral component of myogenesis. To better understand miR-1/miR-206 function during myoblast differentiation we identified novel target mRNAs by microarray and characterized their function in C2C12 myoblasts. Candidate tar ...
Early signaling events that underlie fate decisions of naive CD4 T
... IL-21 or the IL-21R results in diminished Th17 differentiation both in vitro and in vivo (16, 18, 19). If Treg cells are depleted by anti-CD25 treatment prior to immunization, endogenous IL-21 is capable of inducing Th17 responses in vivo even in the absence of IL-6, although to a lesser degree than ...
... IL-21 or the IL-21R results in diminished Th17 differentiation both in vitro and in vivo (16, 18, 19). If Treg cells are depleted by anti-CD25 treatment prior to immunization, endogenous IL-21 is capable of inducing Th17 responses in vivo even in the absence of IL-6, although to a lesser degree than ...
Anabolic Steroids
... Treatment of asthma: Steroids and other anti-inflamitory drugs are used to help control asthma and prevent asthma attacks ...
... Treatment of asthma: Steroids and other anti-inflamitory drugs are used to help control asthma and prevent asthma attacks ...
Jamming prokaryotic cell-to-cell communications in a model biofilm†
... Quorum sensing (QS) is a prime example of paracrine signaling in which a cell affects gene expression in a neighboring cell.1 According to the classic QS hypothesis, bacteria communicate and count their numbers by producing, releasing, and detecting small, diffusible, signaling molecules called auto ...
... Quorum sensing (QS) is a prime example of paracrine signaling in which a cell affects gene expression in a neighboring cell.1 According to the classic QS hypothesis, bacteria communicate and count their numbers by producing, releasing, and detecting small, diffusible, signaling molecules called auto ...
Suppression of Pyk2 Kinase and Cellular Activities by FIP200
... tyrosine kinases with unique structural features. These two kinases exhibit ⵑ45% amino acid identity and they both lack the Src homology 2 or 3 domains that are present in many other cytoplasmic tyrosine kinases. Both Pyk2 and FAK have large NH2- and COOH-terminal noncatalytic domains that flank a c ...
... tyrosine kinases with unique structural features. These two kinases exhibit ⵑ45% amino acid identity and they both lack the Src homology 2 or 3 domains that are present in many other cytoplasmic tyrosine kinases. Both Pyk2 and FAK have large NH2- and COOH-terminal noncatalytic domains that flank a c ...
Arsenic Trioxide-Dependent Activation of Thousand-and
... TAK1 are upstream effectors in the p38 MAPK pathway in response to stress stimuli (Chen and Cobb, 2001; Huangfu et al., 2006). Because the p38 MAPK pathway is activated in an arsenic-dependent manner in leukemia cell lines (Verma et al., 2002; Giafis et al., 2006; Kannan-Thulasiraman et al., 2006; D ...
... TAK1 are upstream effectors in the p38 MAPK pathway in response to stress stimuli (Chen and Cobb, 2001; Huangfu et al., 2006). Because the p38 MAPK pathway is activated in an arsenic-dependent manner in leukemia cell lines (Verma et al., 2002; Giafis et al., 2006; Kannan-Thulasiraman et al., 2006; D ...
Pap and HPV Testing
... 11. What are the limitations of cervical cancer screening? Although cervical cancer screening tests are highly effective, they are not completely accurate. Sometimes a patient can be told that she has abnormal cells when the cells are actually normal (a false-positive result), or she can be told tha ...
... 11. What are the limitations of cervical cancer screening? Although cervical cancer screening tests are highly effective, they are not completely accurate. Sometimes a patient can be told that she has abnormal cells when the cells are actually normal (a false-positive result), or she can be told tha ...
equisetum - Natuurtijdschriften
... trichoblast is helicoidalwith the sive lamellae and with the ...
... trichoblast is helicoidalwith the sive lamellae and with the ...
Effects of dietary components on Tight junctions (TJ) Lauric acid
... nigrum), green pepper, nutmeg, and bay leaf extracts caused an increase in TEER, although small molecule permeability and ZO-1 organization were not affected. The active compound in sweet pepper was identified as capsianoside, and this was shown to reorganize actin filaments and decrease TEER (79). ...
... nigrum), green pepper, nutmeg, and bay leaf extracts caused an increase in TEER, although small molecule permeability and ZO-1 organization were not affected. The active compound in sweet pepper was identified as capsianoside, and this was shown to reorganize actin filaments and decrease TEER (79). ...
From DEPARTMENT OF MEDICAL
... and mDA differentiation defects. This phenotype was phenocopied by mir200c knockdown, indicating that the Zeb2-miR200c loop prevents the premature differentiation of mDA progenitors into postmitotic cells and their migration. Paper IV: We demonstrate that the extracellular matrix protein, laminin 51 ...
... and mDA differentiation defects. This phenotype was phenocopied by mir200c knockdown, indicating that the Zeb2-miR200c loop prevents the premature differentiation of mDA progenitors into postmitotic cells and their migration. Paper IV: We demonstrate that the extracellular matrix protein, laminin 51 ...
Essential embryonic roles of the CKI-1 cyclin
... cycle arrest, morphogenesis and differentiation, and suppression of programmed cell death. The vertebrate Cip/Kip CKIs have been shown to directly inhibit the activity of cyclin/cyclin-dependent kinase (cdk) complexes that control G1/S progression, leading to G1 arrest (Sherr and Roberts, 1995). The ...
... cycle arrest, morphogenesis and differentiation, and suppression of programmed cell death. The vertebrate Cip/Kip CKIs have been shown to directly inhibit the activity of cyclin/cyclin-dependent kinase (cdk) complexes that control G1/S progression, leading to G1 arrest (Sherr and Roberts, 1995). The ...
