Role of N-linked oligosaccharide chains in the processing and
... SDS-PAGE. (a) Single-site mutants. (b) Double-site mutants. (c) Triple- and quadruple-site mutants. Only the relevant section of the autoradiogram is shown. ...
... SDS-PAGE. (a) Single-site mutants. (b) Double-site mutants. (c) Triple- and quadruple-site mutants. Only the relevant section of the autoradiogram is shown. ...
Loss of mitochondrial membrane potential triggers the
... In the budding yeast Saccharomyces cerevisiae, loss of mitochondrial DNA (rho0 ) can induce the retrograde response under appropriate conditions, resulting in increased replicative lifespan (RLS). Although the retrograde pathway has been extensively elaborated, the nature of the mitochondrial signal ...
... In the budding yeast Saccharomyces cerevisiae, loss of mitochondrial DNA (rho0 ) can induce the retrograde response under appropriate conditions, resulting in increased replicative lifespan (RLS). Although the retrograde pathway has been extensively elaborated, the nature of the mitochondrial signal ...
Neural differentiation from embryonic stem cells in vitro: An overview
... sequences bound with SMAD1, SMAD4, and SMAD5. They found that these molecules were associated with many developmental regulators and these were abundant in terms of H3K4 and H3K27 trimethylation bivalent markers. These promoters were found to be repressed when cells were in the self-renewing state, ...
... sequences bound with SMAD1, SMAD4, and SMAD5. They found that these molecules were associated with many developmental regulators and these were abundant in terms of H3K4 and H3K27 trimethylation bivalent markers. These promoters were found to be repressed when cells were in the self-renewing state, ...
Engagement of the inhibitory receptor CD158a
... CTL/RCC-DM conjugates were observed at this level of resolution. Fig. 3A shows a typical image of interface between CTL and RCC-DM tumor cell with strong lipid rafts concentration at the contact zone with the tumor cell, that colocalizes in the overlay picture with the dense red anti-Ptyr staining ( ...
... CTL/RCC-DM conjugates were observed at this level of resolution. Fig. 3A shows a typical image of interface between CTL and RCC-DM tumor cell with strong lipid rafts concentration at the contact zone with the tumor cell, that colocalizes in the overlay picture with the dense red anti-Ptyr staining ( ...
IGF-1 induces rat glomerular mesangial cells to accumulate
... Cell culture. Rat glomerular MC were prepared by modification (4, 5) of routine methods (47). In brief, minced rat kidney cortex was sieved. Isolated glomeruli were plated in medium containing a 1:1 mix of 20% FCS-RPMI 1640 and previously collected glomerular conditioned medium. Insulin routinely ad ...
... Cell culture. Rat glomerular MC were prepared by modification (4, 5) of routine methods (47). In brief, minced rat kidney cortex was sieved. Isolated glomeruli were plated in medium containing a 1:1 mix of 20% FCS-RPMI 1640 and previously collected glomerular conditioned medium. Insulin routinely ad ...
Heat Shock Responses for Understanding Diseases of
... H2O2 and that the oligomerized HSF1 can bind to DNA (Ahn and Thiele, 2003). However, others claim that oxidation of HSF inhibits its DNA binding activity and all of the cysteine mutants of HSF1 could be activated and bind to DNA (Jacquier-Sarlin and Polla, 1996; Manalo et al., 2002). Also, heat shoc ...
... H2O2 and that the oligomerized HSF1 can bind to DNA (Ahn and Thiele, 2003). However, others claim that oxidation of HSF inhibits its DNA binding activity and all of the cysteine mutants of HSF1 could be activated and bind to DNA (Jacquier-Sarlin and Polla, 1996; Manalo et al., 2002). Also, heat shoc ...
Inducibility and Expression of Microvascular
... [20]. In the preculture specimens, endothelial cells did not express ELAM-l (0.5% ± 0.5% of vessels) or VCAM-l (0.7% ± 0.7% of vessels) molecules, consistent with previous reports [15,21]. The percentage of CD36+ vessel cross-sections within the SVP ranged from 15% to 25%, which is identical to that ...
... [20]. In the preculture specimens, endothelial cells did not express ELAM-l (0.5% ± 0.5% of vessels) or VCAM-l (0.7% ± 0.7% of vessels) molecules, consistent with previous reports [15,21]. The percentage of CD36+ vessel cross-sections within the SVP ranged from 15% to 25%, which is identical to that ...
Ultrastructure of diaphragm from dystrophic α-sarcoglycan
... situated close to very small ones (myotubes, atrophic fibres or split fibre fragments) (Fig. 1A, B). In contrast, the size of diaphragm muscle fibres from control mice was relatively uniform (Fig. 1C, D). Several muscle fibres of the Sgca-null mice, especially those of larger size, were strongly basophi ...
... situated close to very small ones (myotubes, atrophic fibres or split fibre fragments) (Fig. 1A, B). In contrast, the size of diaphragm muscle fibres from control mice was relatively uniform (Fig. 1C, D). Several muscle fibres of the Sgca-null mice, especially those of larger size, were strongly basophi ...
Intrahepatic expression of the hepatic stellate cell marker ®broblast
... SMA-positive HSCs, although only a minority will develop cirrhosis (9,14). Other markers of human HSCs have been identi®ed that may identify phenotypic subsets more closely associated with the presence of ®brosis. Recently, we reported ®broblast activation protein (FAP) expression on a subset of act ...
... SMA-positive HSCs, although only a minority will develop cirrhosis (9,14). Other markers of human HSCs have been identi®ed that may identify phenotypic subsets more closely associated with the presence of ®brosis. Recently, we reported ®broblast activation protein (FAP) expression on a subset of act ...
Making a firm decision: multifaceted regulation of cell fate in the
... the apical from basal regions by the blastocyst stage, and this will result in formation of the polarized epithelium of the trophectoderm and the apolar ICM45. As asym‑ metric divisions position cells differentially and result in differential inheritance of their polarization proper‑ ties, it become ...
... the apical from basal regions by the blastocyst stage, and this will result in formation of the polarized epithelium of the trophectoderm and the apolar ICM45. As asym‑ metric divisions position cells differentially and result in differential inheritance of their polarization proper‑ ties, it become ...
Introduction to Fermentation
... E. coli will contain a different gene that is responsible for producing the desired co-protein. The modified E. coli cells will be separately grown through the process of batch fermentation. This tutorial will introduce you to the following areas regarding batch fermentation: • Microbial Growth Phas ...
... E. coli will contain a different gene that is responsible for producing the desired co-protein. The modified E. coli cells will be separately grown through the process of batch fermentation. This tutorial will introduce you to the following areas regarding batch fermentation: • Microbial Growth Phas ...
The tRNA methyltransferase Dnmt2 is required foraccurate
... (Goll et al, 2006) were backcrossed into the C57BL/6 background for more than 10 generations, thus establishing a homogeneous genetic background for the evaluation of specific aberrant phenotypes. The resulting mice were viable and fertile with no overt phenotypic abnormalities. However, a detailed ...
... (Goll et al, 2006) were backcrossed into the C57BL/6 background for more than 10 generations, thus establishing a homogeneous genetic background for the evaluation of specific aberrant phenotypes. The resulting mice were viable and fertile with no overt phenotypic abnormalities. However, a detailed ...
Genetic Regulation of Embryonic Pattern Formation
... daughter cells linked to their different developmental fates? One of the genes that is expressed asymmetrically in the daughter cells of the zygote, PINFORMED7 (PIN7), encodes a member of the PIN family, which presumably are part of the auxin efflux transport machinery (Gälweiler et al., 1998; Frim ...
... daughter cells linked to their different developmental fates? One of the genes that is expressed asymmetrically in the daughter cells of the zygote, PINFORMED7 (PIN7), encodes a member of the PIN family, which presumably are part of the auxin efflux transport machinery (Gälweiler et al., 1998; Frim ...
Stimulation of taxol production by combined salicylic acid elicitation and... Taxus baccata Ayatollah Rezaei
... exogenous SA treatment improved lipid peroxidation rate in plant cell cultures [23]. In addition, MDA content was significantly higher in US-treated microalga Porphyridium cruentum compared to control [24]. Comparing the figures 1 and 2 shows that more the level of MDA, more the decrease in biomass ...
... exogenous SA treatment improved lipid peroxidation rate in plant cell cultures [23]. In addition, MDA content was significantly higher in US-treated microalga Porphyridium cruentum compared to control [24]. Comparing the figures 1 and 2 shows that more the level of MDA, more the decrease in biomass ...
Sugar-Regulated Expression of a Putative
... the untreated control (Fig. 5B), whereas Fru did not induce any rise in GUS activity. Palatinose, a nontransported analog of Suc (M’Batchi and Delrot, 1988) was as effective as Suc and Glc in promoting GUS activity (Fig. 5B). The same sugar sensitivity was found for the GUS activity of p0.3VvHT1-GUS ...
... the untreated control (Fig. 5B), whereas Fru did not induce any rise in GUS activity. Palatinose, a nontransported analog of Suc (M’Batchi and Delrot, 1988) was as effective as Suc and Glc in promoting GUS activity (Fig. 5B). The same sugar sensitivity was found for the GUS activity of p0.3VvHT1-GUS ...
Apigenin
... that apigenin inhibits expression of vascular endothelial growth factor (VEGF) in human ovarian cancer cells. VEGF plays an important role in tumor angiogenesis and growth. We found that apigenin inhibited VEGF expression at the transcriptional level through expression of hypoxia-inducible factor 1a ...
... that apigenin inhibits expression of vascular endothelial growth factor (VEGF) in human ovarian cancer cells. VEGF plays an important role in tumor angiogenesis and growth. We found that apigenin inhibited VEGF expression at the transcriptional level through expression of hypoxia-inducible factor 1a ...
Formative Cell Divisions: Principal Determinants of Plant
... oriented cell divisions form ‘principal determinants’ of multicellular morphogenesis. Symmetric cell divisions with median cell division planes create two daughters of identical sizes and/or fates. This allows amplification of cell populations. Asymmetric cell divisions, on the other hand, with medi ...
... oriented cell divisions form ‘principal determinants’ of multicellular morphogenesis. Symmetric cell divisions with median cell division planes create two daughters of identical sizes and/or fates. This allows amplification of cell populations. Asymmetric cell divisions, on the other hand, with medi ...
Aspects of growth factor signal transduction in the cell cytoplasm
... tyrosine 771 is required for the efficient binding of GAP. Fantl et al. (1992) and Kashishian et al. (1992) ascribed the methionine or valine residues at positions +1 and the methionine residues +3 or +4 relative to the C-terminal phosphotyrosines 731 and 751 of the PDGFR as possibly specifying bind ...
... tyrosine 771 is required for the efficient binding of GAP. Fantl et al. (1992) and Kashishian et al. (1992) ascribed the methionine or valine residues at positions +1 and the methionine residues +3 or +4 relative to the C-terminal phosphotyrosines 731 and 751 of the PDGFR as possibly specifying bind ...
transcriptome analyses reveal differential gene - ePIC
... and can also serve to take up material from the extracellular space for trophic purposes (i.e., phagotrophy). Especially in protists, phagotrophy plays a key role, as it provided (and still provides) a prerequisite for heterotrophic feeding and the evolution of photoautotrophy in eukaryotes (Raven 1 ...
... and can also serve to take up material from the extracellular space for trophic purposes (i.e., phagotrophy). Especially in protists, phagotrophy plays a key role, as it provided (and still provides) a prerequisite for heterotrophic feeding and the evolution of photoautotrophy in eukaryotes (Raven 1 ...
Humic matter elicits proton and calcium fluxes and signaling
... showed by Trevisan et al. [14] using the DR5::GUS construct in Arabidopsis, and evidence for their involvement in plasma membrane H+-ATPase activation has been recently reported [7,15]. This enzyme creates the electrochemical gradient of H+ that energizes secondary transport processes, including ion ...
... showed by Trevisan et al. [14] using the DR5::GUS construct in Arabidopsis, and evidence for their involvement in plasma membrane H+-ATPase activation has been recently reported [7,15]. This enzyme creates the electrochemical gradient of H+ that energizes secondary transport processes, including ion ...
VEGF gene therapy: therapeutic angiogenesis in the clinic
... observation that VEGF-A has acted as a powerful angiogenic factor in small and large animal models of ischemia immediately prompted its utilization in the clinic, also considering the immense medical and socio-economical impact of cardiovascular disorders. Here, we review the outcome of VEGF-based c ...
... observation that VEGF-A has acted as a powerful angiogenic factor in small and large animal models of ischemia immediately prompted its utilization in the clinic, also considering the immense medical and socio-economical impact of cardiovascular disorders. Here, we review the outcome of VEGF-based c ...
cleeks o` cytokinesis: microtubule sticks and contractile hoops in cell
... RacGAP50C ought to provoke ectopic furrowing. To test this, we fused an integral plasma membrane component, the human T-cell receptor CD8, with the full coding region of RacGAP50C and a GFP tag was inserted between the two genes to follow its distribution (Figure 3A). When we induced the expression ...
... RacGAP50C ought to provoke ectopic furrowing. To test this, we fused an integral plasma membrane component, the human T-cell receptor CD8, with the full coding region of RacGAP50C and a GFP tag was inserted between the two genes to follow its distribution (Figure 3A). When we induced the expression ...
The Cell Cycle
... metaphase to anaphase, and allows the cell to complete mitosis. A characteristic common to both yeast and mammalian cells is that they must grow before they can divide. The cytoplasmic volume, and the requisite complement of proteins, and other neces sary molecules must be doubled, along with the ce ...
... metaphase to anaphase, and allows the cell to complete mitosis. A characteristic common to both yeast and mammalian cells is that they must grow before they can divide. The cytoplasmic volume, and the requisite complement of proteins, and other neces sary molecules must be doubled, along with the ce ...