THE PHAGOCYTOSIS AND INACTIVATION OF
... Macrophage Suspensions.--Mononucleax cell exudates were produced in the peritoneal cavity of rabbits by the injection of 50 ml. of sterile heavy mineral oil. The content of granulocytes in exudates produced in this way varies with the time of harvest and the irritant properties of the oil. Some exud ...
... Macrophage Suspensions.--Mononucleax cell exudates were produced in the peritoneal cavity of rabbits by the injection of 50 ml. of sterile heavy mineral oil. The content of granulocytes in exudates produced in this way varies with the time of harvest and the irritant properties of the oil. Some exud ...
Staphylococcus aureus , and Streptococcus pneumoniae , coli
... chamber. Interestingly, the effects of cycloheximide varied substantially according to the stimulus. Migration to intact S. pneumoniae did not require new protein synthesis with migration after cycloheximide at 98.9% of nontreated monolayers. Migration to E. coli was substantially inhibited (69.3% o ...
... chamber. Interestingly, the effects of cycloheximide varied substantially according to the stimulus. Migration to intact S. pneumoniae did not require new protein synthesis with migration after cycloheximide at 98.9% of nontreated monolayers. Migration to E. coli was substantially inhibited (69.3% o ...
UBXD4, a UBX-Containing Protein, Regulates the Cell Surface
... against GenBank (NIH, genetic sequence database) to identify putative ␣3-interacting proteins. The sequencing of a positive isolated clone was identified as an 589 nt reading frame encoding the N terminus of UBXD4 (clone 42). To verify interactions, clone 42 as well as full-length UBXD4 (see plasmid ...
... against GenBank (NIH, genetic sequence database) to identify putative ␣3-interacting proteins. The sequencing of a positive isolated clone was identified as an 589 nt reading frame encoding the N terminus of UBXD4 (clone 42). To verify interactions, clone 42 as well as full-length UBXD4 (see plasmid ...
Receptor Activation and Homer Differentially Control the Lateral
... receptor (between the Ser-22 and Ser-23 codons of mGluR5a, amino acid 1 being the initiating methionine) or just before the stop codon for the C -terminal epitope-tagged receptor, using a PCR overlap extension method. Sense and antisense oligonucleotides coding the c-myc epitope (TREQK LISEEDL AR) w ...
... receptor (between the Ser-22 and Ser-23 codons of mGluR5a, amino acid 1 being the initiating methionine) or just before the stop codon for the C -terminal epitope-tagged receptor, using a PCR overlap extension method. Sense and antisense oligonucleotides coding the c-myc epitope (TREQK LISEEDL AR) w ...
Proteolytic Enzymes from Extremely Halophilic Bacteria
... where they often become the dominating type of organisms when the salt concentration rises above 25 %. The extremely halophilic bacteria are best cultivated in complex media containing peptones and yeast extract to which at least 15 % (wlv) NaCl has been added. Best growth is obtained in media conta ...
... where they often become the dominating type of organisms when the salt concentration rises above 25 %. The extremely halophilic bacteria are best cultivated in complex media containing peptones and yeast extract to which at least 15 % (wlv) NaCl has been added. Best growth is obtained in media conta ...
Dictyostelium lysosomal proteins with different sugar modifications
... and CPs cofractionate with lysosomes and endosomes. GlcNAc-1-P-modified proteins reside in functional vesicles of the endo-lysosomal pathway Magnetic fractionation of vesicles containing iron-coated dextran has been used to purify and characterize functional Dictyostelium lysosomes and endosomes (Ro ...
... and CPs cofractionate with lysosomes and endosomes. GlcNAc-1-P-modified proteins reside in functional vesicles of the endo-lysosomal pathway Magnetic fractionation of vesicles containing iron-coated dextran has been used to purify and characterize functional Dictyostelium lysosomes and endosomes (Ro ...
Electrochemical detection of biological reactions using a novel nano
... on a silicon chip. Each of the electrochemical cells can be monitored simultaneously and independently, and each cell contains three embedded electrodes, which enable performance of all types of electrochemical measurements. The integration of living organisms on an electrochemical array chip that c ...
... on a silicon chip. Each of the electrochemical cells can be monitored simultaneously and independently, and each cell contains three embedded electrodes, which enable performance of all types of electrochemical measurements. The integration of living organisms on an electrochemical array chip that c ...
PowerPoint 프레젠테이션
... the host from invasion by microorganisms and eliminate debris at sites of tissue injury, they can also be responsible for significant tissue damage. • Thus, regulatory mechanisms that limit damage from an overly exuberant immune response have evolved. • It is increasingly apparent that adenosine, a ...
... the host from invasion by microorganisms and eliminate debris at sites of tissue injury, they can also be responsible for significant tissue damage. • Thus, regulatory mechanisms that limit damage from an overly exuberant immune response have evolved. • It is increasingly apparent that adenosine, a ...
- Wiley Online Library
... structures and thus easily destroyed during fixation and drying procedures during preparation for electron microscopy. Interactions with bacteria further increase these degradative processes. To stabilize the BPB-induced neutrophil TVE in some experiments, we used sulfatide from bovine brain as desc ...
... structures and thus easily destroyed during fixation and drying procedures during preparation for electron microscopy. Interactions with bacteria further increase these degradative processes. To stabilize the BPB-induced neutrophil TVE in some experiments, we used sulfatide from bovine brain as desc ...
Hematopoietic cells expressing the peripheral cannabinoid receptor
... exon-1B/exon-2–expressing 32D/G-CSF-R cells. Ligands were added to the lower chamber, and cells that migrated to the lower well were counted after 4 hours of incubation. Y-axis shows the percentage of migrated cells from an input of 1 ⫻ 105. (B) Effect of different concentrations of 2-AG when added ...
... exon-1B/exon-2–expressing 32D/G-CSF-R cells. Ligands were added to the lower chamber, and cells that migrated to the lower well were counted after 4 hours of incubation. Y-axis shows the percentage of migrated cells from an input of 1 ⫻ 105. (B) Effect of different concentrations of 2-AG when added ...
biobugs - Boston University
... Using a wooden stick, smear a small amount of bacteria from the Petri dish onto a clean microscope slide Label the slide using the Sharpie marker Add 3 drops of H2O2 solution onto the smear. Record your observations in the worksheet (Pg. 19). Repeat steps 1-4 for all FIVE bacteria samples, using new ...
... Using a wooden stick, smear a small amount of bacteria from the Petri dish onto a clean microscope slide Label the slide using the Sharpie marker Add 3 drops of H2O2 solution onto the smear. Record your observations in the worksheet (Pg. 19). Repeat steps 1-4 for all FIVE bacteria samples, using new ...
VCE_MFST1500-4
... organisms whose growth on foods usually can be recognized. The main part of the growth commonly is white, but it may be colored, dark, or smokey. Colored spores are typical of some types of mature mold growth and may give color to part or all of the growth. Holds normally reproduce by spore formatio ...
... organisms whose growth on foods usually can be recognized. The main part of the growth commonly is white, but it may be colored, dark, or smokey. Colored spores are typical of some types of mature mold growth and may give color to part or all of the growth. Holds normally reproduce by spore formatio ...
Isolation and Pure Culture of a Freshwater Magnetic Spirillum in
... organism oriented to the vertical as well as horizontal component of the geomagnetic field (A. J. Kalmijn and R. P. Blakemore, Proc. Int. Union Physiol. Sci. 13:364, 1977). Subsequent studies revealed that magnetotactic cells each possessed an intrinsic magnetic dipole moment (11). For biochemical a ...
... organism oriented to the vertical as well as horizontal component of the geomagnetic field (A. J. Kalmijn and R. P. Blakemore, Proc. Int. Union Physiol. Sci. 13:364, 1977). Subsequent studies revealed that magnetotactic cells each possessed an intrinsic magnetic dipole moment (11). For biochemical a ...
ANTIBACTERIAL ACTIVITY OF HUMAN NATURAL KILLER CELLS
... ml samples of appropriate 10-fold dilutions were plated onto tryptic soy agar, incubated overnight at 37 ° C, and the number of CFU was determined . No broth enrichment for defective but surviving bacteria was performed . All experiments were performed in triplicate. Antibacterial activity was expre ...
... ml samples of appropriate 10-fold dilutions were plated onto tryptic soy agar, incubated overnight at 37 ° C, and the number of CFU was determined . No broth enrichment for defective but surviving bacteria was performed . All experiments were performed in triplicate. Antibacterial activity was expre ...
"Neurotransmitter Receptors in the Postsynaptic Neuron". In
... itself has structural (but not amino acid sequence!) homology to the protein bacteriorhodopsin, and homology with rhodopsin, the light-harvesting protein expressed in the eye. Metabotropic neurotransmitter receptors are, mostly, single subunit receptors. Each receptor has seven transmembrane-spannin ...
... itself has structural (but not amino acid sequence!) homology to the protein bacteriorhodopsin, and homology with rhodopsin, the light-harvesting protein expressed in the eye. Metabotropic neurotransmitter receptors are, mostly, single subunit receptors. Each receptor has seven transmembrane-spannin ...
Datasheet - Creative Diagnostics
... azide, which has been classified as Xn (Harmful) in European Directive 67/548/EEC in the concentration range of 0.1-1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains. ...
... azide, which has been classified as Xn (Harmful) in European Directive 67/548/EEC in the concentration range of 0.1-1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains. ...
Polarization of Endocytosis and Receptor
... Membrane receptors for phagocytic particles are not confined to the protuberance but rather may display their own unique topographical asymmetry. The inherent topography of receptors was inferred from particle distribution under conditions that limit particle-receptor redistribution (after labeling ...
... Membrane receptors for phagocytic particles are not confined to the protuberance but rather may display their own unique topographical asymmetry. The inherent topography of receptors was inferred from particle distribution under conditions that limit particle-receptor redistribution (after labeling ...
Boya
... however, it is best to remain non-committal, reporting only gram-positive cocci. Bot genera may be present, but it is particularly important not to direct attention away the possible presence of staphylococci by reporting streptococci even if they are t predominant form, as the staphylococci will no ...
... however, it is best to remain non-committal, reporting only gram-positive cocci. Bot genera may be present, but it is particularly important not to direct attention away the possible presence of staphylococci by reporting streptococci even if they are t predominant form, as the staphylococci will no ...
Signaling
... Receptor/Ligand Binds to promoter elements in DNA and regulates transcription Cortisol Receptor is located in the cytosol ...
... Receptor/Ligand Binds to promoter elements in DNA and regulates transcription Cortisol Receptor is located in the cytosol ...
A feeling for the micro-organism: structure on a small scale. Biofilms
... with a mucilage layer around the root. 3, strings of bacteria between the root epidermal cells. 4, a root hair in the older part of the root. Reprinted with permission from Hansen et al. (1997). Early colonization of barley roots by Pseudomonas fluorescens studied by immunofluorescence technique and ...
... with a mucilage layer around the root. 3, strings of bacteria between the root epidermal cells. 4, a root hair in the older part of the root. Reprinted with permission from Hansen et al. (1997). Early colonization of barley roots by Pseudomonas fluorescens studied by immunofluorescence technique and ...
pdf
... Planktonic microbial communities often appear stable over periods of days and thus tight links are assumed to exist between different functional groups (i.e. producers and consumers). We examined these links by characterizing short-term temporal correspondences in the concentrations and activities o ...
... Planktonic microbial communities often appear stable over periods of days and thus tight links are assumed to exist between different functional groups (i.e. producers and consumers). We examined these links by characterizing short-term temporal correspondences in the concentrations and activities o ...
Oligomerization of Epidermal Growth Factor Receptors on A431
... binds to RAF-l-kinase, thereby initiating the MAP-kinase cascade resulting in the direct phosphorylation of transcription factors and ultimately eliciting the primary mitogenic response, stimulation of DNA replication, and cell division (34, 36). The regulation by EGF of cellular proliferation const ...
... binds to RAF-l-kinase, thereby initiating the MAP-kinase cascade resulting in the direct phosphorylation of transcription factors and ultimately eliciting the primary mitogenic response, stimulation of DNA replication, and cell division (34, 36). The regulation by EGF of cellular proliferation const ...
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... correctly, these drugs are extremely effec8ve at reducing mortality rates and healing injected through a syringe atomizer into a stirring aqueous solution of ampicillin sodium salt in PBS (blue). The organic solvent and any unencapsulated drug are subsequently remo ...
... correctly, these drugs are extremely effec8ve at reducing mortality rates and healing injected through a syringe atomizer into a stirring aqueous solution of ampicillin sodium salt in PBS (blue). The organic solvent and any unencapsulated drug are subsequently remo ...
ß-arrestin signaling and regulation of transcription
... proteins encoded in the human genome whose functions have been predicted, 616 (>4%) are members of the rhodopsin, secretin or metabotropic glutamate receptor class of GPCR (Venter et al., 2001). Several decades of extensive studies have revealed that these receptors play fundamental roles in regulat ...
... proteins encoded in the human genome whose functions have been predicted, 616 (>4%) are members of the rhodopsin, secretin or metabotropic glutamate receptor class of GPCR (Venter et al., 2001). Several decades of extensive studies have revealed that these receptors play fundamental roles in regulat ...
Chemotaxis
Chemotaxis (from chemo- + taxis) is the movement of an organism in response to a chemical stimulus. Somatic cells, bacteria, and other single-cell or multicellular organisms direct their movements according to certain chemicals in their environment. This is important for bacteria to find food (e.g., glucose) by swimming toward the highest concentration of food molecules, or to flee from poisons (e.g., phenol). In multicellular organisms, chemotaxis is critical to early development (e.g., movement of sperm towards the egg during fertilization) and subsequent phases of development (e.g., migration of neurons or lymphocytes) as well as in normal function. In addition, it has been recognized that mechanisms that allow chemotaxis in animals can be subverted during cancer metastasis.Positive chemotaxis occurs if the movement is toward a higher concentration of the chemical in question; negative chemotaxis if the movement is in the opposite direction. Chemically prompted kinesis (randomly directed or nondirectional) can be called chemokinesis.