Differential function of Listeria monocytogenes listeriolysin O and
... medium and grown for 5 h at 37°C in the presence of various concentrations of IPTG. Wild-type strain 10403S was grown under similar conditions in the absence of IPTG. Haemolytic activity present in culture supernatants was determined as described in Experimental procedures. HU values represent the m ...
... medium and grown for 5 h at 37°C in the presence of various concentrations of IPTG. Wild-type strain 10403S was grown under similar conditions in the absence of IPTG. Haemolytic activity present in culture supernatants was determined as described in Experimental procedures. HU values represent the m ...
Journal of Bacteriology
... we reported that both cellulose fibrils and a Ca2+-dependent adhesin of Rhizobium leguminosarum bv. viciae cells are involved in the two-step process of attachment of rhizobia to pea root hair tips (13). In the study reported here, the influence of Ca2" limitation on motility and surface components ...
... we reported that both cellulose fibrils and a Ca2+-dependent adhesin of Rhizobium leguminosarum bv. viciae cells are involved in the two-step process of attachment of rhizobia to pea root hair tips (13). In the study reported here, the influence of Ca2" limitation on motility and surface components ...
1 An amoeba phagocytosis model reveals a novel developmental
... be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Insect Physiology, vol 59, issue ...
... be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Insect Physiology, vol 59, issue ...
The chemokine SDF1/CXCL12 and its receptor CXCR4 regulate
... (C) Cxcr4 message is enriched in PGCs relative to the somatic tissue. The level of Cxcr4 transcripts in E11.5 PGCs (GFP+) or somatic tissue (GFP–) were quantified by SYBR-green based RT-PCR. The meiotic marker, STAG3 (Pezzi et al., 2000) was used as a positive control for PGCs, and the gonadal marke ...
... (C) Cxcr4 message is enriched in PGCs relative to the somatic tissue. The level of Cxcr4 transcripts in E11.5 PGCs (GFP+) or somatic tissue (GFP–) were quantified by SYBR-green based RT-PCR. The meiotic marker, STAG3 (Pezzi et al., 2000) was used as a positive control for PGCs, and the gonadal marke ...
Xenopus laevis Stromal Cell-Derived Factor 1
... and feline SDF-1 are identical in amino acid sequence (15, 16). This high degree of sequence identity between species has previously been taken to suggest that almost all SDF-1 residues are required for biological activity. Although the genes for other known hCXC chemokines are located on chromosome ...
... and feline SDF-1 are identical in amino acid sequence (15, 16). This high degree of sequence identity between species has previously been taken to suggest that almost all SDF-1 residues are required for biological activity. Although the genes for other known hCXC chemokines are located on chromosome ...
The polar flagellum mediates Azuspidlum brasifense
... Azospirilkcm bnrsilense in a motile Gram-negative bacterium that can adapt its flagellation to different environments. Cells growing in a liquid culture possess only a single polar flagellum; growth on a solid surface additionally induces multiple lateral flagella. The polar flagellum is primarily u ...
... Azospirilkcm bnrsilense in a motile Gram-negative bacterium that can adapt its flagellation to different environments. Cells growing in a liquid culture possess only a single polar flagellum; growth on a solid surface additionally induces multiple lateral flagella. The polar flagellum is primarily u ...
Protist predation can favour cooperation within bacterial species
... The costs and benefits of cooperation and cheating are dependent on environmental context [1–3]. Here, we investigate how a ubiquitous selection pressure, predation, affects selection for cooperation in the bacterium Pseudomonas aeruginosa, which cooperates by secreting and responding to quorum sens ...
... The costs and benefits of cooperation and cheating are dependent on environmental context [1–3]. Here, we investigate how a ubiquitous selection pressure, predation, affects selection for cooperation in the bacterium Pseudomonas aeruginosa, which cooperates by secreting and responding to quorum sens ...
Boundary formation in the hindbrain
... Eph receptors are the largest subfamily of receptor tyrosine kinases [a]. Their ligands, the ephrins, are membranebound, either via a glycosyl phosphatidylinositol linkage (ephrin-A ligands) or via integral transmembrane and intracellular domains (ephrin-B ligands) [b]. Eph receptors are subdivided ...
... Eph receptors are the largest subfamily of receptor tyrosine kinases [a]. Their ligands, the ephrins, are membranebound, either via a glycosyl phosphatidylinositol linkage (ephrin-A ligands) or via integral transmembrane and intracellular domains (ephrin-B ligands) [b]. Eph receptors are subdivided ...
Material ecologies for synthetic biology
... of isolated gene sequences resulting in clearly defined characteristics in an organism, much of what we understand in terms of the morphology and behavior of biological systems is derived from groups of different genes being expressed through the more complex (compared to the genome) proteome—the en ...
... of isolated gene sequences resulting in clearly defined characteristics in an organism, much of what we understand in terms of the morphology and behavior of biological systems is derived from groups of different genes being expressed through the more complex (compared to the genome) proteome—the en ...
Bacterial flagella explore microscale hummocks and hollows to
... medium. These static cultures were incubated for 24 h at 37 °C and then prepared for scanning electron microscope imaging (Fig. 1 C and D). Surprisingly, our observations indicated that there was more surface coverage by the E. coli cells on HEX than on flat surfaces. Furthermore, we noted the presen ...
... medium. These static cultures were incubated for 24 h at 37 °C and then prepared for scanning electron microscope imaging (Fig. 1 C and D). Surprisingly, our observations indicated that there was more surface coverage by the E. coli cells on HEX than on flat surfaces. Furthermore, we noted the presen ...
Transforming growth factor–1 in supernatants from stored red blood
... Consistent with the possible increased risk of postoperative infections in transfusion patients, various studies suggest that transfusions can induce immunomodulatory effects, including transient reduction in the CD4/CD8 T-cell ratio, reduced natural killer function, impaired lymphocyte mitogenic re ...
... Consistent with the possible increased risk of postoperative infections in transfusion patients, various studies suggest that transfusions can induce immunomodulatory effects, including transient reduction in the CD4/CD8 T-cell ratio, reduced natural killer function, impaired lymphocyte mitogenic re ...
Full_CAD_Artical - Synbio.Construction
... of isolated gene sequences resulting in clearly defined characteristics in an organism, much of what we understand in terms of the morphology and behavior of biological systems is derived from groups of different genes being expressed through the more complex (compared to the genome) proteome—the en ...
... of isolated gene sequences resulting in clearly defined characteristics in an organism, much of what we understand in terms of the morphology and behavior of biological systems is derived from groups of different genes being expressed through the more complex (compared to the genome) proteome—the en ...
Week 5 Lab
... the thick peptidoglycan layer which traps the crystal violet - iodine inside the cell. Thus Gram + bacteria appear purple in the end. If you over decolorize it is possible to wash out the normally trapped crystal violet - iodine from inside the cell. That is why the decolorization step is critical. ...
... the thick peptidoglycan layer which traps the crystal violet - iodine inside the cell. Thus Gram + bacteria appear purple in the end. If you over decolorize it is possible to wash out the normally trapped crystal violet - iodine from inside the cell. That is why the decolorization step is critical. ...
Protistology First evidence of bacterial endocytobionts in the lobose
... amoeba genera described. The features making them an ideal research object are the flat, transparent organic test and the relatively large size. Some data are available on their culture conditions (Netzel, 1975). Arcella species can be easily collected in the nature and the identification of the morph ...
... amoeba genera described. The features making them an ideal research object are the flat, transparent organic test and the relatively large size. Some data are available on their culture conditions (Netzel, 1975). Arcella species can be easily collected in the nature and the identification of the morph ...
Protein Kinase C–dependent Activation of Cytosolic
... receptors. Three groups of mammalian PLA2s have been characterized, namely, the 14-kD Ca21-dependent secreted PLA2s, the 85-kD Ca21-dependent and sn-2 arachidonyl-specific cytosolic PLA2 (cPLA2), and the Ca21-independent PLA2s (3, 4). Overexpression of Chinese hamster ovary cells with recombinant cP ...
... receptors. Three groups of mammalian PLA2s have been characterized, namely, the 14-kD Ca21-dependent secreted PLA2s, the 85-kD Ca21-dependent and sn-2 arachidonyl-specific cytosolic PLA2 (cPLA2), and the Ca21-independent PLA2s (3, 4). Overexpression of Chinese hamster ovary cells with recombinant cP ...
Jordan Ciccone 9th Grade Pittsburgh Central Catholic Highschool
... -Proteus -Pseudomonas -Staphylococcus (aureus and epidermidis) ...
... -Proteus -Pseudomonas -Staphylococcus (aureus and epidermidis) ...
Distribution and prevalence of bacteria found on the door handles of
... care centers, and other public places. The study also highlighted the variety of potential pathogens. According to Salton, Gram negative bacteria show virulent characteristics due to the presence of endotoxin in their outer membranes. Endotoxin causes activation of immune system cells such as macrop ...
... care centers, and other public places. The study also highlighted the variety of potential pathogens. According to Salton, Gram negative bacteria show virulent characteristics due to the presence of endotoxin in their outer membranes. Endotoxin causes activation of immune system cells such as macrop ...
T Cell Interstitial Migration: Motility Cues from the Inflamed Tissue for
... interpret the cacophony of signals coming from their tissue locale into “go” signals during migration and “stop” signals for cell–cell interactions is yet to be fully understood (Figure 1). T cells must traverse their immediate tissue terrain (micro-positioning) as well as accumulate at specific foc ...
... interpret the cacophony of signals coming from their tissue locale into “go” signals during migration and “stop” signals for cell–cell interactions is yet to be fully understood (Figure 1). T cells must traverse their immediate tissue terrain (micro-positioning) as well as accumulate at specific foc ...
Acanthamoeba polyphaga Intracellular Infection of
... the late stages of this intracellular infection at the ultrastructural level. Therefore, we examined the integrity of the phagosomal membrane throughout intracellular infection of U937 macrophages and Acanthamoeba polyphaga at the ultrastructural level. In addition, we examined the presence of the l ...
... the late stages of this intracellular infection at the ultrastructural level. Therefore, we examined the integrity of the phagosomal membrane throughout intracellular infection of U937 macrophages and Acanthamoeba polyphaga at the ultrastructural level. In addition, we examined the presence of the l ...
Tonic and burst firing: dual modes of
... tonic mode so that the new object could be analyzed more faithfully. It is also plausible that bursts activate EPSPs more effectively in their target cortical cells, because recent evidence suggests that an action potential in a geniculate cell will probably activate its cortical target if it follow ...
... tonic mode so that the new object could be analyzed more faithfully. It is also plausible that bursts activate EPSPs more effectively in their target cortical cells, because recent evidence suggests that an action potential in a geniculate cell will probably activate its cortical target if it follow ...
Growth of bacteria in seawater filtered through 0.2 pm Nuclepore
... study indicate that this assumption cannot be taken for granted when diluent is prepared by vacuum filtration (< 100 mm Hg pressure) of natural seawater through 0.2 pm Nuclepore filters. Although the number of bacteria and heterotrophic activity in freshly prepared ...
... study indicate that this assumption cannot be taken for granted when diluent is prepared by vacuum filtration (< 100 mm Hg pressure) of natural seawater through 0.2 pm Nuclepore filters. Although the number of bacteria and heterotrophic activity in freshly prepared ...
Coca Cola
... Rho dependent stress fiber formation Internal (localization dependent) signals ADF/Cofilin ...
... Rho dependent stress fiber formation Internal (localization dependent) signals ADF/Cofilin ...
Molecular Cloning and Pharmacological Characterization of a
... bioamine receptors resulted in the isolation of several partial receptor cDNA fragments that are expressed in the CNS of the pond snail L. stagnalis. One of these fragments showed considerable similarity to the vertebrate a-adrenergic receptors. A full-length cDNA clone (3416 bp) corresponding to th ...
... bioamine receptors resulted in the isolation of several partial receptor cDNA fragments that are expressed in the CNS of the pond snail L. stagnalis. One of these fragments showed considerable similarity to the vertebrate a-adrenergic receptors. A full-length cDNA clone (3416 bp) corresponding to th ...
Receptor Cell Biology: Receptor-Mediated Endocytosis
... Figure 3. Receptor and ligand are segregated within endosome/CURL. Immunoelectron micrographs of ultrathin cryosections from rat liver labeled with two sizes of colloidal gold. Livers were continuously infused with asialofetuin 1 h before fixation. (a) A vesicle just beneath the sinusoidal ( S ) pla ...
... Figure 3. Receptor and ligand are segregated within endosome/CURL. Immunoelectron micrographs of ultrathin cryosections from rat liver labeled with two sizes of colloidal gold. Livers were continuously infused with asialofetuin 1 h before fixation. (a) A vesicle just beneath the sinusoidal ( S ) pla ...
Applied and Environmental Microbiology
... taken 96 h after inoculation of the rhizosphere with the gfp-marked strain. The samples were observed with an epifluorescence microscope or placed on King’s B medium plates containing kanamycin (50 g/ml) and incubated for 1 min prior to crushing. The samples were then removed, and the plates were i ...
... taken 96 h after inoculation of the rhizosphere with the gfp-marked strain. The samples were observed with an epifluorescence microscope or placed on King’s B medium plates containing kanamycin (50 g/ml) and incubated for 1 min prior to crushing. The samples were then removed, and the plates were i ...
Chemotaxis
Chemotaxis (from chemo- + taxis) is the movement of an organism in response to a chemical stimulus. Somatic cells, bacteria, and other single-cell or multicellular organisms direct their movements according to certain chemicals in their environment. This is important for bacteria to find food (e.g., glucose) by swimming toward the highest concentration of food molecules, or to flee from poisons (e.g., phenol). In multicellular organisms, chemotaxis is critical to early development (e.g., movement of sperm towards the egg during fertilization) and subsequent phases of development (e.g., migration of neurons or lymphocytes) as well as in normal function. In addition, it has been recognized that mechanisms that allow chemotaxis in animals can be subverted during cancer metastasis.Positive chemotaxis occurs if the movement is toward a higher concentration of the chemical in question; negative chemotaxis if the movement is in the opposite direction. Chemically prompted kinesis (randomly directed or nondirectional) can be called chemokinesis.