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... (MWCNTs). (a) MWCNT powder; (b) probe‐sonicated MWCNT suspension, air‐dried. Both samples were imaged at a 15‐kV accelerating voltage with a gaseous secondary electron detector in wet mode (3 torr); scale bar is 1 μm. MWCNTs appeared less tangled after probe‐sonication in Nanopure water, reflect ...
... (MWCNTs). (a) MWCNT powder; (b) probe‐sonicated MWCNT suspension, air‐dried. Both samples were imaged at a 15‐kV accelerating voltage with a gaseous secondary electron detector in wet mode (3 torr); scale bar is 1 μm. MWCNTs appeared less tangled after probe‐sonication in Nanopure water, reflect ...
Mechanism of Phagocytosis in Phagocytosis is Mediated by
... Quantitation of Endocytotic Uptake The major experimental difficulty in measuring initial rates of phagocytosis is to find a rapid procedure to separate quantitatively cells containing ingested material from the bulk of uningested material . Separation by differential centrifugation, the procedure u ...
... Quantitation of Endocytotic Uptake The major experimental difficulty in measuring initial rates of phagocytosis is to find a rapid procedure to separate quantitatively cells containing ingested material from the bulk of uningested material . Separation by differential centrifugation, the procedure u ...
Biological synthesis of fluorescent nanoparticles by cadmium and
... Background: Fluorescent nanoparticles or quantum dots (QDs) have been intensely studied for basic and applied research due to their unique size-dependent properties. There is an increasing interest in developing ecofriendly methods to synthesize these nanoparticles since they improve biocompatibili ...
... Background: Fluorescent nanoparticles or quantum dots (QDs) have been intensely studied for basic and applied research due to their unique size-dependent properties. There is an increasing interest in developing ecofriendly methods to synthesize these nanoparticles since they improve biocompatibili ...
Pathogenesis of the Human Opportunistic Pathogen
... suspension using a syringe (E). A, Confocal scanning micrograph of GFP-labeled PA14 concentrated in substomatal cavity 24 hpi as seen in the same focal plane as the parenchyma cells. Strong red autofluorescence from chloroplasts is readily apparent. Bar ⫽ 10 m. B, Syto 9-stained leaf cross section ...
... suspension using a syringe (E). A, Confocal scanning micrograph of GFP-labeled PA14 concentrated in substomatal cavity 24 hpi as seen in the same focal plane as the parenchyma cells. Strong red autofluorescence from chloroplasts is readily apparent. Bar ⫽ 10 m. B, Syto 9-stained leaf cross section ...
EXPERIMENT 3 - UniMAP Portal
... a differential staining procedure. Once bacteria have been properly stained, it is usually an easy matter to discern their overall shape. Bacterial morphology is usually uncomplicated and limited to one of a few variations. ...
... a differential staining procedure. Once bacteria have been properly stained, it is usually an easy matter to discern their overall shape. Bacterial morphology is usually uncomplicated and limited to one of a few variations. ...
INACTIVATING aiiA GENE HOMOLOGUE IN
... Quorum sensing (QS) in bacteria acts as a sophisticated circuit to detect and respond to the environmental changes as well as to group up the cells together by cell-cell communication within or between the species (Waters and Bassler 2005). Various bacteria use this communication pathway to coordina ...
... Quorum sensing (QS) in bacteria acts as a sophisticated circuit to detect and respond to the environmental changes as well as to group up the cells together by cell-cell communication within or between the species (Waters and Bassler 2005). Various bacteria use this communication pathway to coordina ...
The main actors involved in extending the invertebrate life span
... demonstrated that the presence of E. coli induced modifications in the fat body. This organ was characterized by a loose tissue of both layers of cells close to the integument and different sized lobes surrounding the internal organs in thorax and abdomen cavities. The most abundant cell type consis ...
... demonstrated that the presence of E. coli induced modifications in the fat body. This organ was characterized by a loose tissue of both layers of cells close to the integument and different sized lobes surrounding the internal organs in thorax and abdomen cavities. The most abundant cell type consis ...
Full text in pdf format
... parameters were quantified and compared with the abundance, biomass and bacterivory of the heterotrophic nanoflagellate (HNF) community. We observed pronounced shifts within 2 distinct size ranges of the bactenoplankton. Short, rod-shaped cells between 0.4 and 1.2 pm cell length formed between 30 to ...
... parameters were quantified and compared with the abundance, biomass and bacterivory of the heterotrophic nanoflagellate (HNF) community. We observed pronounced shifts within 2 distinct size ranges of the bactenoplankton. Short, rod-shaped cells between 0.4 and 1.2 pm cell length formed between 30 to ...
Hybridoma and Hybridomics
... The cells were fixed in PFA, as described by Amann et al.(13) The fixed cells were permeabilized by digestion with lysozyme (662 U/mL PBS; 15 min; room temperature). The reaction was stopped by two washes in PBS. Some droplets of the cell suspension were transferred to a glass slide and dried by roo ...
... The cells were fixed in PFA, as described by Amann et al.(13) The fixed cells were permeabilized by digestion with lysozyme (662 U/mL PBS; 15 min; room temperature). The reaction was stopped by two washes in PBS. Some droplets of the cell suspension were transferred to a glass slide and dried by roo ...
Spontaneous zygogenesis in Escherichia coli, a form
... Cultures of parental strains growing exponentially in LB were diluted in M9 and mixed at a cell density that did not exceed 26107 c.f.u. ml21. Mixtures were incubated at 37 uC under gentle linear rocking. The incubation time was 20 min before plating on the selective medium, MA, LCA or both. In time ...
... Cultures of parental strains growing exponentially in LB were diluted in M9 and mixed at a cell density that did not exceed 26107 c.f.u. ml21. Mixtures were incubated at 37 uC under gentle linear rocking. The incubation time was 20 min before plating on the selective medium, MA, LCA or both. In time ...
The Care and Feeding of the Microscope Lab 5
... between two points that are close together, so that they are seen as separate units; it is the ability of the lenses to distinguish fine detail and structure (or a measure of the clarity of the image). Example: A microscope with a resolving power of 0.4 nm can distinguish between two points if the ...
... between two points that are close together, so that they are seen as separate units; it is the ability of the lenses to distinguish fine detail and structure (or a measure of the clarity of the image). Example: A microscope with a resolving power of 0.4 nm can distinguish between two points if the ...
Statistical Analysis of the Patterns of Spore Formation
... occurs at a critical point 15to 20 min into a round of chromosome replication (Mandelstam & Higgs, 1974). Now, if there is an immediate response to resuspension, that is necessary for sporulation, this response cannot be equated with ‘initiation’ (as used by Mandelstam & Higgs, 1974). However, recen ...
... occurs at a critical point 15to 20 min into a round of chromosome replication (Mandelstam & Higgs, 1974). Now, if there is an immediate response to resuspension, that is necessary for sporulation, this response cannot be equated with ‘initiation’ (as used by Mandelstam & Higgs, 1974). However, recen ...
BioWire_Progress_Report,_Week_One
... [Components will be ordered Monday] Replicate Weiss sender cell, adding GFP as a reporter Manually add aTc to the system in varying concentrations Control: Add water instead of aTc Expected Result: GFP is expressed, but not in control plates. ...
... [Components will be ordered Monday] Replicate Weiss sender cell, adding GFP as a reporter Manually add aTc to the system in varying concentrations Control: Add water instead of aTc Expected Result: GFP is expressed, but not in control plates. ...
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... • Complement is a group of serum proteins – activated in an orderly fashion from inactive forms. This leads to “spin-off” peptides that have biological activity. • Three main activation pathways and one terminal pathway that leads to the formation of the MAC. • Complement activation is highly regula ...
... • Complement is a group of serum proteins – activated in an orderly fashion from inactive forms. This leads to “spin-off” peptides that have biological activity. • Three main activation pathways and one terminal pathway that leads to the formation of the MAC. • Complement activation is highly regula ...
Assessment of antimicrobial compounds by microscopy techniques
... of health departments and pharmaceutical industries to develop new lead compounds to fight against persistent infections. Many bacteria resistance strategies rely on the guard keeping of membrane and cell wall integrity. Antimicrobial proteins and peptides (AMPPs) are among the selected lead compoun ...
... of health departments and pharmaceutical industries to develop new lead compounds to fight against persistent infections. Many bacteria resistance strategies rely on the guard keeping of membrane and cell wall integrity. Antimicrobial proteins and peptides (AMPPs) are among the selected lead compoun ...
PDF - compatibile with Acrobat 3.0
... The origin of bioluminescence is regarded as a problematic aspect of Darwin’s theory of evolution (for recent discussions see Czyż & Węgrzyn 2001a, Labas et al. 2001). The problem is that the function of bioluminescence is generally believed to be directly associated with the visual behaviour of org ...
... The origin of bioluminescence is regarded as a problematic aspect of Darwin’s theory of evolution (for recent discussions see Czyż & Węgrzyn 2001a, Labas et al. 2001). The problem is that the function of bioluminescence is generally believed to be directly associated with the visual behaviour of org ...
Counting and imaging bacteria using
... properties, but their effects on bacterial morphology are not well known. Objective: Use the AFM to probe morphological changes in response to chemical treatments. ...
... properties, but their effects on bacterial morphology are not well known. Objective: Use the AFM to probe morphological changes in response to chemical treatments. ...
Breaking dogmas: the plant vascular pathogen Xanthomonas
... and (iii) transmission electron microscopy (TEM). We demonstrate that X. albilineans is not only able to infect and to move within the xylem, but is also able to invade apparently intact parenchymatous cells, as well as other non-vascular cells, a feature that has not been described for any plant pa ...
... and (iii) transmission electron microscopy (TEM). We demonstrate that X. albilineans is not only able to infect and to move within the xylem, but is also able to invade apparently intact parenchymatous cells, as well as other non-vascular cells, a feature that has not been described for any plant pa ...
CN Theveneau
... (facial), IX (glossopharyngeal), and X (vagus). Alongside with ectodermal placodes, they also contribute to neurones of the ciliary (CN III/oculomotor), trigeminal (CN V/ trigeminal), and vestibular (CN VIII/vestibulocochlear) ganglia. Distal ganglia of the CN VII (geniculate), IX (petrosal), and X ...
... (facial), IX (glossopharyngeal), and X (vagus). Alongside with ectodermal placodes, they also contribute to neurones of the ciliary (CN III/oculomotor), trigeminal (CN V/ trigeminal), and vestibular (CN VIII/vestibulocochlear) ganglia. Distal ganglia of the CN VII (geniculate), IX (petrosal), and X ...
- Wiley Online Library
... (facial), IX (glossopharyngeal), and X (vagus). Alongside with ectodermal placodes, they also contribute to neurones of the ciliary (CN III/oculomotor), trigeminal (CN V/ trigeminal), and vestibular (CN VIII/vestibulocochlear) ganglia. Distal ganglia of the CN VII (geniculate), IX (petrosal), and X ...
... (facial), IX (glossopharyngeal), and X (vagus). Alongside with ectodermal placodes, they also contribute to neurones of the ciliary (CN III/oculomotor), trigeminal (CN V/ trigeminal), and vestibular (CN VIII/vestibulocochlear) ganglia. Distal ganglia of the CN VII (geniculate), IX (petrosal), and X ...
Dynamics of intracellular bacterial replication at the single cell level
... killing indices of the two bacterial strains in WT and phox−/− macrophages. In these experiments, bacteria were also sampled at earlier times post-uptake, when the respiratory burst is more pronounced (Fig. 2C). As expected, phox-dependent killing was detected at early (0.5 h to 6 h) but not late (6 ...
... killing indices of the two bacterial strains in WT and phox−/− macrophages. In these experiments, bacteria were also sampled at earlier times post-uptake, when the respiratory burst is more pronounced (Fig. 2C). As expected, phox-dependent killing was detected at early (0.5 h to 6 h) but not late (6 ...
the ubiquity of bacteria
... THE UBIQUITY OF BACTERIA Bacteria far outnumber all other life forms on the planet. In fact, in your large intestine alone you harbor more bacterial cells than the total number of human cells in your body. It is estimated that only 3% of bacteria are pathogenic for man and animals. Bacteria are foun ...
... THE UBIQUITY OF BACTERIA Bacteria far outnumber all other life forms on the planet. In fact, in your large intestine alone you harbor more bacterial cells than the total number of human cells in your body. It is estimated that only 3% of bacteria are pathogenic for man and animals. Bacteria are foun ...
Dendritic cells express tight junction proteins and penetrate gut
... 3c) and both inside and outside (Fig. 3b) DCs and during the DC internalization process (Fig. 3d). At this time-point (45 min into coincubation) we could count, per filter, an average of 1550±160 bacteria that were associated with DCs, of which one-third were internalized. We also found that there w ...
... 3c) and both inside and outside (Fig. 3b) DCs and during the DC internalization process (Fig. 3d). At this time-point (45 min into coincubation) we could count, per filter, an average of 1550±160 bacteria that were associated with DCs, of which one-third were internalized. We also found that there w ...
Cells Eukaryotes and Prokaryotes Life - Eukaryotes
... Flagella - flagella of motile bacteria differ in structure from eukaryotic flagella. A basal body anchored in the plasma membrane and cell wall gives rise to a cylindrical protein filament. The flagellum moves by whirling about its long axis. The number and arrangement of flagella on the cell are di ...
... Flagella - flagella of motile bacteria differ in structure from eukaryotic flagella. A basal body anchored in the plasma membrane and cell wall gives rise to a cylindrical protein filament. The flagellum moves by whirling about its long axis. The number and arrangement of flagella on the cell are di ...
Cellular Membranes
... • Some carriers passively transport substances down their respective concentration gradient • Other carriers actively transport substances up their respective concentration gradient – carriers called pumps hydrolyze a molecule of ATP and use the energy to pump substances across the membrane against ...
... • Some carriers passively transport substances down their respective concentration gradient • Other carriers actively transport substances up their respective concentration gradient – carriers called pumps hydrolyze a molecule of ATP and use the energy to pump substances across the membrane against ...
Chemotaxis
Chemotaxis (from chemo- + taxis) is the movement of an organism in response to a chemical stimulus. Somatic cells, bacteria, and other single-cell or multicellular organisms direct their movements according to certain chemicals in their environment. This is important for bacteria to find food (e.g., glucose) by swimming toward the highest concentration of food molecules, or to flee from poisons (e.g., phenol). In multicellular organisms, chemotaxis is critical to early development (e.g., movement of sperm towards the egg during fertilization) and subsequent phases of development (e.g., migration of neurons or lymphocytes) as well as in normal function. In addition, it has been recognized that mechanisms that allow chemotaxis in animals can be subverted during cancer metastasis.Positive chemotaxis occurs if the movement is toward a higher concentration of the chemical in question; negative chemotaxis if the movement is in the opposite direction. Chemically prompted kinesis (randomly directed or nondirectional) can be called chemokinesis.