Ionotropic Glutamate Receptors in Aplysia californica and Molecular
... and α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), based on selective agonists. The latter two subtypes are known as non-NMDA receptors (nonNMDAR). Overall amino acid identity of iGluR subunits across the three families in mammals is 20-30%, but each contain common structural features ...
... and α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), based on selective agonists. The latter two subtypes are known as non-NMDA receptors (nonNMDAR). Overall amino acid identity of iGluR subunits across the three families in mammals is 20-30%, but each contain common structural features ...
Analysis of Connexin43 phosphorylated at S325, S328 and S330 in
... indicate that this polyclonal antibody can bind to Cx43 phosphorylated at residues S325, S328 and S330, and also that Cx43 is phosphorylated at S328 and/or S330, and to a lesser extent at S325 in heart tissue. The role of phosphorylation at S325, S328 and S330 in gap junction assembly To be more qua ...
... indicate that this polyclonal antibody can bind to Cx43 phosphorylated at residues S325, S328 and S330, and also that Cx43 is phosphorylated at S328 and/or S330, and to a lesser extent at S325 in heart tissue. The role of phosphorylation at S325, S328 and S330 in gap junction assembly To be more qua ...
PDF
... axis formation. The hallmark of canonical Wnt signaling is the translocation of β-catenin into the nucleus where it activates gene transcription. However, the mechanisms regulating β-catenin nuclear localization are poorly understood. We show that Simplet/ Fam53B (Smp) is required for Wnt signaling ...
... axis formation. The hallmark of canonical Wnt signaling is the translocation of β-catenin into the nucleus where it activates gene transcription. However, the mechanisms regulating β-catenin nuclear localization are poorly understood. We show that Simplet/ Fam53B (Smp) is required for Wnt signaling ...
Mammalian Models of CNS Regeneration - Wiley-VCH
... Although Nogo-66, MAG and OMgp lack sequence homology, a single cell-surface protein, NgR1 acts as a functional receptor for all three molecules (Fournier et al., 2001; Liu et al., 2002; Wang et al., 2002a). NgR1 is a GPI-linked cell-surface protein and requires LINGO-1 and either p75 or TROY for si ...
... Although Nogo-66, MAG and OMgp lack sequence homology, a single cell-surface protein, NgR1 acts as a functional receptor for all three molecules (Fournier et al., 2001; Liu et al., 2002; Wang et al., 2002a). NgR1 is a GPI-linked cell-surface protein and requires LINGO-1 and either p75 or TROY for si ...
video slide - Rushford-Peterson Community Education
... Transcriptional Regulation • Specific transcription factors bind directly to specific regions of DNA and control transcription of genes • Positive transcription factors are proteins that increase the transcription of specific genes, while negative transcription factors are proteins that decrease th ...
... Transcriptional Regulation • Specific transcription factors bind directly to specific regions of DNA and control transcription of genes • Positive transcription factors are proteins that increase the transcription of specific genes, while negative transcription factors are proteins that decrease th ...
Lecture
... • Positive transcription factors are proteins that increase the transcription of specific genes, while negative transcription factors are proteins that decrease the transcription of specific genes ...
... • Positive transcription factors are proteins that increase the transcription of specific genes, while negative transcription factors are proteins that decrease the transcription of specific genes ...
Coupling transcription, splicing and mRNA export
... conserved mRNA export pathway and the EJC, a new Drosophila RNA interference (RNAi) study by Gatfield and Izaurralde [14] concludes that, although both Tap and UAP56 are required for mRNA export, Aly and the other EJC components are dispensable. The authors propose that an as-yet undiscovered adapto ...
... conserved mRNA export pathway and the EJC, a new Drosophila RNA interference (RNAi) study by Gatfield and Izaurralde [14] concludes that, although both Tap and UAP56 are required for mRNA export, Aly and the other EJC components are dispensable. The authors propose that an as-yet undiscovered adapto ...
Response
... Transcriptional Regulation • Specific transcription factors bind directly to specific regions of DNA and control transcription of genes • Positive transcription factors are proteins that increase the transcription of specific genes, while negative transcription factors are proteins that decrease th ...
... Transcriptional Regulation • Specific transcription factors bind directly to specific regions of DNA and control transcription of genes • Positive transcription factors are proteins that increase the transcription of specific genes, while negative transcription factors are proteins that decrease th ...
Effects of the Metabotropic Glutamate Receptor Antagonist MCPG
... consequence of PI turnover is the generation of two second messengers, diacylglycerol and phosphatidylinositol trisphosphate, leading in turn to activation of protein kinase C and the release of intracellular calcium stores (Berridge, 1984). Thus, it has been suggested that differences in the patter ...
... consequence of PI turnover is the generation of two second messengers, diacylglycerol and phosphatidylinositol trisphosphate, leading in turn to activation of protein kinase C and the release of intracellular calcium stores (Berridge, 1984). Thus, it has been suggested that differences in the patter ...
Rapid Reversion from Monomer to Dimer
... of UVR8 dimer and monomer following UV-B treatment of intact wild-type Arabidopsis plants. As shown in Figure 1A, essentially all the UVR8 protein is present as a dimer before UV-B exposure, and it is then converted to the monomer in response to a 3-h UV-B treatment. When plants are subsequently tra ...
... of UVR8 dimer and monomer following UV-B treatment of intact wild-type Arabidopsis plants. As shown in Figure 1A, essentially all the UVR8 protein is present as a dimer before UV-B exposure, and it is then converted to the monomer in response to a 3-h UV-B treatment. When plants are subsequently tra ...
Ribosome Profiling Provides Evidence that Large Please share
... (“genes encoding typical-size proteins”), (ii) the translated regions of 639 known proteincoding transcripts encoding a protein with <100 amino acids (“genes encoding small proteins”), and (iii) 130 well-established non-coding RNAs that are well expressed in mouse ES cells (referred to as “classical ...
... (“genes encoding typical-size proteins”), (ii) the translated regions of 639 known proteincoding transcripts encoding a protein with <100 amino acids (“genes encoding small proteins”), and (iii) 130 well-established non-coding RNAs that are well expressed in mouse ES cells (referred to as “classical ...
Mechanisms of plasmid stable maintenance with special focus on
... the probability of receiving a plasmid by a dividing cell there are very special strategies adopted by plasmids preventing plasmid-free segregants from surviving. The idea of an “addiction” mechanism leading to very efficient plasmid maintenance comes from Koyama (Koyama et al., 1975). In his consid ...
... the probability of receiving a plasmid by a dividing cell there are very special strategies adopted by plasmids preventing plasmid-free segregants from surviving. The idea of an “addiction” mechanism leading to very efficient plasmid maintenance comes from Koyama (Koyama et al., 1975). In his consid ...
Ryanodine receptors: next generation of insecticide targets
... reticulum of muscle cells and neurons. They are the largest ion channels known made up of four monomers, each 565kDa in size. Mammals have 3 different RyR isoforms, encoded by different genes, while insects express only one isoform of the receptor, which is only 46% similar (at the amino acid level) ...
... reticulum of muscle cells and neurons. They are the largest ion channels known made up of four monomers, each 565kDa in size. Mammals have 3 different RyR isoforms, encoded by different genes, while insects express only one isoform of the receptor, which is only 46% similar (at the amino acid level) ...
The cell biology of lignification in higher plants
... Background Lignin is a polyphenolic polymer that strengthens and waterproofs the cell wall of specialized plant cell types. Lignification is part of the normal differentiation programme and functioning of specific cell types, but can also be triggered as a response to various biotic and abiotic st ...
... Background Lignin is a polyphenolic polymer that strengthens and waterproofs the cell wall of specialized plant cell types. Lignification is part of the normal differentiation programme and functioning of specific cell types, but can also be triggered as a response to various biotic and abiotic st ...
Characterization of Mg -regulated TRPM7
... i ) and MgATP. By contraries, decreased Mgi (in free or ATP-bound form) up-regulates the current carried by TRPM7 channels [2,8,15,16]. Data from different groups suggest that the altered function and/or regulation of TRPM7 channels could be implicated in pathological conditions such as anoxic cell ...
... i ) and MgATP. By contraries, decreased Mgi (in free or ATP-bound form) up-regulates the current carried by TRPM7 channels [2,8,15,16]. Data from different groups suggest that the altered function and/or regulation of TRPM7 channels could be implicated in pathological conditions such as anoxic cell ...
here - The Nebenführ Lab
... shown). The gradual loss of Golgi stacks and the simultaneous increase in ER-like fluorescence are indicative of a redistribution of the GmMan1-GFP Golgi marker into the ER. Although some of the ER-like fluorescence may have resulted from newly synthesized GFP in the ER, the speed with which the flu ...
... shown). The gradual loss of Golgi stacks and the simultaneous increase in ER-like fluorescence are indicative of a redistribution of the GmMan1-GFP Golgi marker into the ER. Although some of the ER-like fluorescence may have resulted from newly synthesized GFP in the ER, the speed with which the flu ...
Refining the Definition of Plant Mitochondrial
... analysis of 58 experimentally proven plant mitochondrial presequences deposited in the Swiss-Prot database revealed two major classes containing an Arg residue at positions 22 and 23 and one class without any conserved Arg residues (Zhang et al., 2001; Zhang and Glaser, 2002). However, this data set ...
... analysis of 58 experimentally proven plant mitochondrial presequences deposited in the Swiss-Prot database revealed two major classes containing an Arg residue at positions 22 and 23 and one class without any conserved Arg residues (Zhang et al., 2001; Zhang and Glaser, 2002). However, this data set ...
Dynamics of survival of motor neuron (SMN)
... Figure 1 IMP1 is a component of mRNPs localized and actively transported in motor neuron axons. (A) Primary motor neurons (5DIV) were stained with an antibody specific for IMP1 (green). Granular staining was observed in the cell body and dendrites (left panel), proximal axon (top right), and growth ...
... Figure 1 IMP1 is a component of mRNPs localized and actively transported in motor neuron axons. (A) Primary motor neurons (5DIV) were stained with an antibody specific for IMP1 (green). Granular staining was observed in the cell body and dendrites (left panel), proximal axon (top right), and growth ...
DNA-dependent protein kinase interacts functionally with the RNA
... Recruitment of DNA-PK at the HIV LTR As it is known that DNA-PK phosphorylates the CTD of RNAP II (Dvir et al., 1993; Trigon et al., 1998), we performed chromatin immunoprecipitation (ChIP) assays to investigate the possible presence of this enzyme at the HIV LTR. These assays were performed by usin ...
... Recruitment of DNA-PK at the HIV LTR As it is known that DNA-PK phosphorylates the CTD of RNAP II (Dvir et al., 1993; Trigon et al., 1998), we performed chromatin immunoprecipitation (ChIP) assays to investigate the possible presence of this enzyme at the HIV LTR. These assays were performed by usin ...
ref. #29 of the TIBS article
... A number of recent biophysical studies have investigated the apparent affinity of aromatic residues for the lipid-water interface or central hydrophobic regions of phospholipid membranes. Data for small model peptides show that the partitioning of Trp into the interface of lipid bilayers is highly f ...
... A number of recent biophysical studies have investigated the apparent affinity of aromatic residues for the lipid-water interface or central hydrophobic regions of phospholipid membranes. Data for small model peptides show that the partitioning of Trp into the interface of lipid bilayers is highly f ...
Keystone Exam Study Guide
... BIO.B.2.4.1 Explain how genetic engineering has impacted the fields of medicine, forensics, and agriculture (e.g., selective breeding, gene splicing, cloning, genetically modified organisms, gene therapy). ...
... BIO.B.2.4.1 Explain how genetic engineering has impacted the fields of medicine, forensics, and agriculture (e.g., selective breeding, gene splicing, cloning, genetically modified organisms, gene therapy). ...
Proper Folding and Endoplasmic Reticulum to Golgi
... analysis of tyrosinase mRNA showed no increase in message levels (Fig. 1B, compare lane 5 to lane 6), suggesting that the changes occurred post-transcriptionally. Taken together, the increased abundance of tyrosinase species of 70 and 60 kDa and the accumulation of Endo H-resistant glycoforms indica ...
... analysis of tyrosinase mRNA showed no increase in message levels (Fig. 1B, compare lane 5 to lane 6), suggesting that the changes occurred post-transcriptionally. Taken together, the increased abundance of tyrosinase species of 70 and 60 kDa and the accumulation of Endo H-resistant glycoforms indica ...
R on Dendritic Cells - The Journal of Immunology
... trauma to reach acute phase levels as high as 100 –500 g/ml (7, 8). CRP interacts with the immune system by activating the classical complement pathway leading to opsonization (9, 10) and by direct binding to receptors on leukocytes leading to phagocytosis and cytokine synthesis. We established the ...
... trauma to reach acute phase levels as high as 100 –500 g/ml (7, 8). CRP interacts with the immune system by activating the classical complement pathway leading to opsonization (9, 10) and by direct binding to receptors on leukocytes leading to phagocytosis and cytokine synthesis. We established the ...
Signal transduction
Signal transduction occurs when an extracellular signaling molecule activates a specific receptor located on the cell surface or inside the cell. In turn, this receptor triggers a biochemical chain of events inside the cell, creating a response. Depending on the cell, the response alters the cell's metabolism, shape, gene expression, or ability to divide. The signal can be amplified at any step. Thus, one signaling molecule can cause many responses.