articles - Saleh Lab
... but seem to be unable to transmit this dsRNA to other cells in the body4. dsRNA uptake without further transmission to other cells has also been reported for some mammalian cell types5–7. Other organisms (such as C. elegans or juvenile grasshoppers) can both take up dsRNA and spread it systemically ...
... but seem to be unable to transmit this dsRNA to other cells in the body4. dsRNA uptake without further transmission to other cells has also been reported for some mammalian cell types5–7. Other organisms (such as C. elegans or juvenile grasshoppers) can both take up dsRNA and spread it systemically ...
Enzymatic inactivation of endogenous IgG by IdeS enhances
... In contrast to the high affinity receptor, there are three human FcγRs that mainly functionally respond to complexed IgG (CD16, CD32a and CD32b) (12). Their main functions to remove target structures by ADCC and ADCP are also well established in vitro and there is also accumulating evidence for thei ...
... In contrast to the high affinity receptor, there are three human FcγRs that mainly functionally respond to complexed IgG (CD16, CD32a and CD32b) (12). Their main functions to remove target structures by ADCC and ADCP are also well established in vitro and there is also accumulating evidence for thei ...
Pyruvate Kinase
... Global control is for the benefit of the whole organism, & often involves hormone-activated signal cascades. Liver cells have major roles in metabolism, including maintaining blood levels various of nutrients such as glucose. Thus global control especially involves liver. ...
... Global control is for the benefit of the whole organism, & often involves hormone-activated signal cascades. Liver cells have major roles in metabolism, including maintaining blood levels various of nutrients such as glucose. Thus global control especially involves liver. ...
Rat Retinal Pigment Epithelial Cells Show Specificity of
... After particle feeding and rinsing, cells on glass coverslips were fixed in 2 ml of 3.7% formaldehyde in 0.1 M PBS, pH 7.4, for 30 min at 37°C (9). To reduce breakage of attached ROS, the formaldehyde concentration was gradually decreased by serial dilution with PBS, before a final 30-min rinse in P ...
... After particle feeding and rinsing, cells on glass coverslips were fixed in 2 ml of 3.7% formaldehyde in 0.1 M PBS, pH 7.4, for 30 min at 37°C (9). To reduce breakage of attached ROS, the formaldehyde concentration was gradually decreased by serial dilution with PBS, before a final 30-min rinse in P ...
Role of adiponectin in the regulation of carbohydrate and lipid
... peroxisomes (33); and mitochondrial uncoupling protein 2 (UCP2), accompanied ...
... peroxisomes (33); and mitochondrial uncoupling protein 2 (UCP2), accompanied ...
Polysaccharides of higher fungi: biological role, structure and
... and to avoid vitamin degradation [46]. Antioxidants are not only used in the food industry, but also in cosmetology and in the pharmaceutical industry, as well as in the manufacturing of tyres and fuels (gas and biodiesel), in order to prevent oxidative degradation processes. Natural antioxidants, s ...
... and to avoid vitamin degradation [46]. Antioxidants are not only used in the food industry, but also in cosmetology and in the pharmaceutical industry, as well as in the manufacturing of tyres and fuels (gas and biodiesel), in order to prevent oxidative degradation processes. Natural antioxidants, s ...
Regulation of gene expression by Pax6 in ocular cells: a case of
... Pax6, c-Maf, MafA/L-Maf, MafB, NRL, Sox2, Sox1, RARβ These factors are differentially expressed in lens precursor cells, lens epithelium and primary and secondary lens fibers. They exert their function in combination with ubiquitously expressed factors (e.g. AP-1, CREB, pRb, TFIID and USF) and co-ac ...
... Pax6, c-Maf, MafA/L-Maf, MafB, NRL, Sox2, Sox1, RARβ These factors are differentially expressed in lens precursor cells, lens epithelium and primary and secondary lens fibers. They exert their function in combination with ubiquitously expressed factors (e.g. AP-1, CREB, pRb, TFIID and USF) and co-ac ...
A Personal Account of Chaperonin History
... fate of Rubisco. In the cells that over-expressed GroE nearly all of the Rubisco was soluble and biologically active. For this to occur required both GroEL and GroES to be over-expressed. This was true for both L2 and S4 䡠 (L2)4 䡠 S4 forms of Rubisco. We thus concluded that the formation of active R ...
... fate of Rubisco. In the cells that over-expressed GroE nearly all of the Rubisco was soluble and biologically active. For this to occur required both GroEL and GroES to be over-expressed. This was true for both L2 and S4 䡠 (L2)4 䡠 S4 forms of Rubisco. We thus concluded that the formation of active R ...
Desert hedgehogis a mammal-specific gene expressed during
... Hedgehog actions are mediated at the cell surface by a multi-component receptor complex comprising the patched (PTCH) receptors and smoothened (SMO) protein [17]. Both proteins have orthologues in Drosophila that are also involved in hedgehog signal transduction and pattern formation. Initially, all ...
... Hedgehog actions are mediated at the cell surface by a multi-component receptor complex comprising the patched (PTCH) receptors and smoothened (SMO) protein [17]. Both proteins have orthologues in Drosophila that are also involved in hedgehog signal transduction and pattern formation. Initially, all ...
Desert hedgehog is a mammal-specific gene expressed during testicular and ovarian
... Hedgehog actions are mediated at the cell surface by a multi-component receptor complex comprising the patched (PTCH) receptors and smoothened (SMO) protein [17]. Both proteins have orthologues in Drosophila that are also involved in hedgehog signal transduction and pattern formation. Initially, all ...
... Hedgehog actions are mediated at the cell surface by a multi-component receptor complex comprising the patched (PTCH) receptors and smoothened (SMO) protein [17]. Both proteins have orthologues in Drosophila that are also involved in hedgehog signal transduction and pattern formation. Initially, all ...
Carbohydrate Metabolism
... source in almost all living cells, major emphasis is placed on its synthesis, degradation, and storage. iving cells are in a state of ceaseless activity. To maintain its “life,” each cell depends on highly coordinated biochemical reactions. Carbohydrates are an important source of the energy that dr ...
... source in almost all living cells, major emphasis is placed on its synthesis, degradation, and storage. iving cells are in a state of ceaseless activity. To maintain its “life,” each cell depends on highly coordinated biochemical reactions. Carbohydrates are an important source of the energy that dr ...
Properties of a newly characterized protein of the bovine - K-REx
... Reaction of affinity-purified mouse anti-protein X and mouse anti-transacetylase antibodies with the subunits and polypeptides prepared from the kidney or heart pyruvate dehydrogenase complex and with the kidney a-ketoglutarate dehydrogenase complex ...
... Reaction of affinity-purified mouse anti-protein X and mouse anti-transacetylase antibodies with the subunits and polypeptides prepared from the kidney or heart pyruvate dehydrogenase complex and with the kidney a-ketoglutarate dehydrogenase complex ...
Peroxisome Proliferator-Activated Receptor Activation is
... BHMT, DMGDH and SARDH [25]. However, whether TTA treatment affects the related metabolites has yet to be explored. Also, activation of PPARα has been shown to increase the synthesis of vitamin B3 from tryptophan by regulating key enzymes in this pathway [26], but whether PPAR activation influences t ...
... BHMT, DMGDH and SARDH [25]. However, whether TTA treatment affects the related metabolites has yet to be explored. Also, activation of PPARα has been shown to increase the synthesis of vitamin B3 from tryptophan by regulating key enzymes in this pathway [26], but whether PPAR activation influences t ...
Impaired overload-induced muscle growth is associated with
... antibody, stripped, probed for phospho-Thr389 S6k, stripped again, and probed for phospho-Thr421 /Ser424 S6k. Because mTOR protein detection was not effective after stripping, phospho- and total mTOR values were obtained from separate blots using the exact same homogenate-loading buffer preparations ...
... antibody, stripped, probed for phospho-Thr389 S6k, stripped again, and probed for phospho-Thr421 /Ser424 S6k. Because mTOR protein detection was not effective after stripping, phospho- and total mTOR values were obtained from separate blots using the exact same homogenate-loading buffer preparations ...
Introduction to Protein Structure
... – Only a subset of these will fold in a stable conformation ...
... – Only a subset of these will fold in a stable conformation ...
Rb is required for progression through myogenic differentiation but
... (MHC) is reduced. In addition, serum restimulation of these differentiated pRb-deficient myoblasts results in BrdU incorporation and, thus, S-phase entry and DNA synthesis. However, these cells are unable to enter mitosis. Moreover, forced expression of MyoD in a variety of Rb/ fibroblastic cells ...
... (MHC) is reduced. In addition, serum restimulation of these differentiated pRb-deficient myoblasts results in BrdU incorporation and, thus, S-phase entry and DNA synthesis. However, these cells are unable to enter mitosis. Moreover, forced expression of MyoD in a variety of Rb/ fibroblastic cells ...
Lipoteichoic Acid Synthesis and Function in Gram
... enzymes; however, the two-enzyme system found in Listeria spp. appears to be unique, as LtaP has significantly diverged from the LtaS enzyme. In Bacillus spp., which contain four LTA synthase enzymes, these proteins are more closely related to each other and the LtaS protein of L. monocytogenes than ...
... enzymes; however, the two-enzyme system found in Listeria spp. appears to be unique, as LtaP has significantly diverged from the LtaS enzyme. In Bacillus spp., which contain four LTA synthase enzymes, these proteins are more closely related to each other and the LtaS protein of L. monocytogenes than ...
A Novel Form of DAP5 Protein Accumulates in Apoptotic Cells as a
... and others. Altogether, the execution of apoptosis must be tightly regulated. This is achieved by a stringent requirement for an apoptotic trigger on the one hand and protection from inappropriate activation of the cell death program in cells intended to survive on the other hand. The latter mechani ...
... and others. Altogether, the execution of apoptosis must be tightly regulated. This is achieved by a stringent requirement for an apoptotic trigger on the one hand and protection from inappropriate activation of the cell death program in cells intended to survive on the other hand. The latter mechani ...
Transport and Sorting of the Solanum tuberosum Sucrose
... effect on the sucrose transporter activity is due to enhanced proton flux across the membrane mediated by H+-ATPases or other proton pumping activities, we measured the total proton flux across the plant PM in response to various redox reagents via acridine orange quenching (Figure 4A). No increase ...
... effect on the sucrose transporter activity is due to enhanced proton flux across the membrane mediated by H+-ATPases or other proton pumping activities, we measured the total proton flux across the plant PM in response to various redox reagents via acridine orange quenching (Figure 4A). No increase ...
Calcium-induced calcium release supports recruitment of synaptic
... potential contribution of SK-evoked hyperpolarization due to Ca2⫹ release from ER. Reduction during prolonged caffeine application could alternatively be explained by eventual Ca2⫹ depletion in the ER and a consequent impairment of a potential CICR mechanism. Caffeine reduces postsynaptic activity i ...
... potential contribution of SK-evoked hyperpolarization due to Ca2⫹ release from ER. Reduction during prolonged caffeine application could alternatively be explained by eventual Ca2⫹ depletion in the ER and a consequent impairment of a potential CICR mechanism. Caffeine reduces postsynaptic activity i ...
Trans Fatty Acid Derived Phospholipids Show Increased Membrane
... and the membrane receptor rhodopsin in model membranes. Rhodopsin is a prototypical member of the G-protein coupled receptor family. It has a well-characterized structure and function and serves as a model membrane receptor in this study. Phospholipid-cholesterol affinity was quantified by measuring ...
... and the membrane receptor rhodopsin in model membranes. Rhodopsin is a prototypical member of the G-protein coupled receptor family. It has a well-characterized structure and function and serves as a model membrane receptor in this study. Phospholipid-cholesterol affinity was quantified by measuring ...
FGF1 inhibits p53-dependent apoptosis and cell cycle arrest via an
... stimuli: the addition of death factors (TNF, TGFb, Fas ligand), the absence of survival factors (fibroblast growth factors (FGFs), IGF-I, and neurotrophins), genotoxic stresses and activation of the oncosuppressor p53. Whatever the original death signal, most apoptotic pathways converge on activation ...
... stimuli: the addition of death factors (TNF, TGFb, Fas ligand), the absence of survival factors (fibroblast growth factors (FGFs), IGF-I, and neurotrophins), genotoxic stresses and activation of the oncosuppressor p53. Whatever the original death signal, most apoptotic pathways converge on activation ...
Metal ion reconstitution studies of yeast copper
... have an OD258:OD280 ratio of slightly less than 1 : 1, as is in fact observed (see Fig. 2). Protein concentration was also estimated using amino acid analysis and the tyrosinate difference spectral method. From amino acid analysis data, the concentration of each amino acid was divided by the number ...
... have an OD258:OD280 ratio of slightly less than 1 : 1, as is in fact observed (see Fig. 2). Protein concentration was also estimated using amino acid analysis and the tyrosinate difference spectral method. From amino acid analysis data, the concentration of each amino acid was divided by the number ...
Signal transduction
Signal transduction occurs when an extracellular signaling molecule activates a specific receptor located on the cell surface or inside the cell. In turn, this receptor triggers a biochemical chain of events inside the cell, creating a response. Depending on the cell, the response alters the cell's metabolism, shape, gene expression, or ability to divide. The signal can be amplified at any step. Thus, one signaling molecule can cause many responses.