Membrane transporters in a human genome
... when combined with Recon 2. This information will be valuable for further refinements. These data will also provide starting points for further experiments by highlighting areas of incomplete knowledge. This review represents the first comprehensive overview of the transporters involved in central m ...
... when combined with Recon 2. This information will be valuable for further refinements. These data will also provide starting points for further experiments by highlighting areas of incomplete knowledge. This review represents the first comprehensive overview of the transporters involved in central m ...
Capping protein: new insights into mechanism
... high concentrations of protein under conditions in which S100B binds tightly to the 12- and 28-residue a-subunit peptides and to denatured CP [26,28]. Relatively high concentrations of the non-ionic detergent Triton-X100 did allow S100B to bind weakly to CP without denaturing either protein, however ...
... high concentrations of protein under conditions in which S100B binds tightly to the 12- and 28-residue a-subunit peptides and to denatured CP [26,28]. Relatively high concentrations of the non-ionic detergent Triton-X100 did allow S100B to bind weakly to CP without denaturing either protein, however ...
INSULIN RESISTANCE IN H-35 RAT HEPATO~A CELLS IS
... maximal response. The concentration of insulin (measured by radioimmunoassay) remaining in the medium after 24 h of incubation with H-35 cells is 150 ng/ml, which is more than the maximally effective concentration of 20 ng/ml (see Fig. 2, upper curve). At 24 h (Fig. IB) the medium was removed from t ...
... maximal response. The concentration of insulin (measured by radioimmunoassay) remaining in the medium after 24 h of incubation with H-35 cells is 150 ng/ml, which is more than the maximally effective concentration of 20 ng/ml (see Fig. 2, upper curve). At 24 h (Fig. IB) the medium was removed from t ...
A structural comparison of molybdenum cofactor
... each family, sequence similarities are obvious, while no signi¢cant homologies can be detected between members of di¡erent families. This classi¢cation represents a unifying approach in terms of the overall enzyme structure, but, at the same time, it does not require the Mo/W center to be coordinate ...
... each family, sequence similarities are obvious, while no signi¢cant homologies can be detected between members of di¡erent families. This classi¢cation represents a unifying approach in terms of the overall enzyme structure, but, at the same time, it does not require the Mo/W center to be coordinate ...
CHEMICAL SYNTHESIS OF PROTEINS : A TOOL FOR PROTEIN
... Ligation (NCL) appear as the most useful and advantageous strategy [Dawson, P.E. et al. 1994]. The method leads to the formation of a protein molecule through the chemoselective ligation of two peptide segments containing the former a C-terminal α-thioester group and the latter an N-terminal Cys res ...
... Ligation (NCL) appear as the most useful and advantageous strategy [Dawson, P.E. et al. 1994]. The method leads to the formation of a protein molecule through the chemoselective ligation of two peptide segments containing the former a C-terminal α-thioester group and the latter an N-terminal Cys res ...
Pax8, a murine paired box gene expressed in the developing
... The open reading frame of the Pax8 gene The Pax8 cDNA clone c27B contains a large open reading frame that could translate into a protein of 457 amino acids (aa) containing the paired domain near its amino terminus (Figs 1 and 2). This open reading frame begins at nucleotide 156 and ends with a stop ...
... The open reading frame of the Pax8 gene The Pax8 cDNA clone c27B contains a large open reading frame that could translate into a protein of 457 amino acids (aa) containing the paired domain near its amino terminus (Figs 1 and 2). This open reading frame begins at nucleotide 156 and ends with a stop ...
Drosophila as a Model Organism for the Study of
... and still difficult in other vertebrates, including their sophisticated genetics, ease of many cell biological manipulations, less gene redundancy and a very powerful toolbox of transgenic methods for neuronal circuit analysis – and for these reasons are continuing to play a pioneering role in genet ...
... and still difficult in other vertebrates, including their sophisticated genetics, ease of many cell biological manipulations, less gene redundancy and a very powerful toolbox of transgenic methods for neuronal circuit analysis – and for these reasons are continuing to play a pioneering role in genet ...
Cell-cycle-regulated expression of STIL controls centriole number in
... ortholog in vertebrates. In support of this view, all three proteins share sequence similarity within a short, ,90 aa C-terminal domain called the STAN (STIL/Ana2) motif (Stevens et al., 2010). The human STIL gene was first identified in the context of a genomic rearrangement that leads to T-cell ac ...
... ortholog in vertebrates. In support of this view, all three proteins share sequence similarity within a short, ,90 aa C-terminal domain called the STAN (STIL/Ana2) motif (Stevens et al., 2010). The human STIL gene was first identified in the context of a genomic rearrangement that leads to T-cell ac ...
Structure and Function Relationships between ATPase Family, AAA
... order to pass genetic material from one generation to the next; however, DNA-damaging agents constantly injure the genome. High fidelity replication of the cell’s genetic material is normally carried out through the combination of accurate DNA polymerases and DNA mismatch repair (1). These high-fide ...
... order to pass genetic material from one generation to the next; however, DNA-damaging agents constantly injure the genome. High fidelity replication of the cell’s genetic material is normally carried out through the combination of accurate DNA polymerases and DNA mismatch repair (1). These high-fide ...
Transport proteins regulate the flux of metabolites and cofactors
... date, the proteins of two transporter families are known to reside in the peroxisomal membrane: The peroxisomal ATP-binding cassette (ABC) transporter involved in the import of substrates for β-oxidation and three members of the mitochondrial carrier (MC) family required for the influx of the cofacto ...
... date, the proteins of two transporter families are known to reside in the peroxisomal membrane: The peroxisomal ATP-binding cassette (ABC) transporter involved in the import of substrates for β-oxidation and three members of the mitochondrial carrier (MC) family required for the influx of the cofacto ...
Comparison of Methods for Estimating Unbound Intracellular
... with the unbound fraction in liver homogenates (fT,homogenate) measured by equilibrium dialysis using human liver samples. The Kp,uu,ss obtained were then compared with Kp,uu,V0 in both rat and human hepatocytes. The difference between Kp,uu,ss or Kp,uu,V0 and true Kp,uu (Kp,uu,true) is discussed in ...
... with the unbound fraction in liver homogenates (fT,homogenate) measured by equilibrium dialysis using human liver samples. The Kp,uu,ss obtained were then compared with Kp,uu,V0 in both rat and human hepatocytes. The difference between Kp,uu,ss or Kp,uu,V0 and true Kp,uu (Kp,uu,true) is discussed in ...
the article
... to serve as an ‘architectural’ protein22. As it is now appreciated that HMGB1 can be released from cells, we hypothesized that in a way analogous to its binding of nuclear DNA, HMGB1 could bind to extracellular DNA and, by forming HMGB1-DNA complexes, modify the immune-regulatory effects of specific ...
... to serve as an ‘architectural’ protein22. As it is now appreciated that HMGB1 can be released from cells, we hypothesized that in a way analogous to its binding of nuclear DNA, HMGB1 could bind to extracellular DNA and, by forming HMGB1-DNA complexes, modify the immune-regulatory effects of specific ...
lecture 6 ppt
... Given an equation, particularly that for cellular respiration, determine which molecules are oxidized and reduced ...
... Given an equation, particularly that for cellular respiration, determine which molecules are oxidized and reduced ...
A conserved N-‐terminal domain of Rif2 regulates telomere length in
... DNA binding complexes, there are other proteins that associate with telomeres and help regulate both end-‐protection and telomere length. These include a number of proteins that are involved in DNA break ...
... DNA binding complexes, there are other proteins that associate with telomeres and help regulate both end-‐protection and telomere length. These include a number of proteins that are involved in DNA break ...
Mycobacteria Target DC-SIGN to Suppress Dendritic Cell Function
... *Abbreviations used in this paper: BCG, bacillus Calmette-Guérin; DC, dendritic cell; ICAM, intercellular adhesion molecule; LAM, lipoarabi- ...
... *Abbreviations used in this paper: BCG, bacillus Calmette-Guérin; DC, dendritic cell; ICAM, intercellular adhesion molecule; LAM, lipoarabi- ...
Triglyceride-Rich Lipoproteins as Agents of Innate Immunity
... It has been recognized for 120 years that the interaction between LPS and lipoproteins is dependent on plasma factors [8, 13]. Although several plasma proteins can interact with LPS, none have been more extensively studied than CD14 [18] and the acute-phase reactant LPS-binding protein (LBP) [19, 20 ...
... It has been recognized for 120 years that the interaction between LPS and lipoproteins is dependent on plasma factors [8, 13]. Although several plasma proteins can interact with LPS, none have been more extensively studied than CD14 [18] and the acute-phase reactant LPS-binding protein (LBP) [19, 20 ...
Proteins of Human Milk. I. Identification of Major Components
... which may be isolated a nd analyzed (3 , 24-36). Specific surface- differe nt iation an tigens of human mammary epithelial cells have been isolated from membranes of fat globules of human milk (28). Casein micelles and soluble (whey) proteins are collected in to secretory vesicles fo r exocytosis (3 ...
... which may be isolated a nd analyzed (3 , 24-36). Specific surface- differe nt iation an tigens of human mammary epithelial cells have been isolated from membranes of fat globules of human milk (28). Casein micelles and soluble (whey) proteins are collected in to secretory vesicles fo r exocytosis (3 ...
Full Text - Labs / Projects - Fred Hutchinson Cancer Research Center
... 3772 C. R. Magie, D. Pinto-Santini and S. M. Parkhurst number of associated proteins including members of the catenin family: β-catenin (Armadillo), α-catenin, and p120 catenin (p120ctn) (Hatzfeld, 1999; Steinberg and McNutt, 1999). While β-catenin and α-catenin are involved in linking cadherins to ...
... 3772 C. R. Magie, D. Pinto-Santini and S. M. Parkhurst number of associated proteins including members of the catenin family: β-catenin (Armadillo), α-catenin, and p120 catenin (p120ctn) (Hatzfeld, 1999; Steinberg and McNutt, 1999). While β-catenin and α-catenin are involved in linking cadherins to ...
Seed Germination and Dormancy
... Before considering some of the cellular and metabolic aspects of dormancy, its breaking, and the resultant completion of germination, it is worthwhile pondering why so little progress has been made toward understanding dormancy. Undoubtedly, one contributing factor is that we do not know the definin ...
... Before considering some of the cellular and metabolic aspects of dormancy, its breaking, and the resultant completion of germination, it is worthwhile pondering why so little progress has been made toward understanding dormancy. Undoubtedly, one contributing factor is that we do not know the definin ...
2386 East Heritage Way, Suite B, Salt Lake City, Utah 84109 USA
... liver hepatocytes to specific proapoptotic signals including TNF␣ and FasL, a finding that is reproduced in epithelial cell lines (Caulin et al. 2000; Gilbert et al. 2001; Jaquemar et al. 2003; Ku et al. 2003). In the context of primary culture, newborn mouse skin keratinocytes express K17 and K14 u ...
... liver hepatocytes to specific proapoptotic signals including TNF␣ and FasL, a finding that is reproduced in epithelial cell lines (Caulin et al. 2000; Gilbert et al. 2001; Jaquemar et al. 2003; Ku et al. 2003). In the context of primary culture, newborn mouse skin keratinocytes express K17 and K14 u ...
Ceman, S, O Donnell, WT, Reed, M, Patton, S, Pohl, J and Warren, ST: Phosphorylation regulates translation state of FMRP-associated polyribosomes. Human Molecular Genetics 12:3295-3305 (2003).
... detected by precursor ion scanning. (B) Phosphopeptides from murine brain detected by tandem mass spectrometry. All species contain a single phosphorylation site unless otherwise indicated in bold. ...
... detected by precursor ion scanning. (B) Phosphopeptides from murine brain detected by tandem mass spectrometry. All species contain a single phosphorylation site unless otherwise indicated in bold. ...
Analysis of exocyst subunit EXO70 family reveals
... Plant cells have several polar membrane domains with different composition (Žárský et al., 2009, Łangowski et al., 2010 Łangowski et al., 2016) and additional polarized structures arise during response to pathogens and symbionts (Dormann et al., 2014).One of the key components regulating cell polari ...
... Plant cells have several polar membrane domains with different composition (Žárský et al., 2009, Łangowski et al., 2010 Łangowski et al., 2016) and additional polarized structures arise during response to pathogens and symbionts (Dormann et al., 2014).One of the key components regulating cell polari ...
Subcellular trafficking kinetics of GLUT4 mutated at the N
... case of TGN38, both a transmembrane domain and a β-turn type of motif [17,18]. GLUT4 has several potential targeting domains and evidence has been presented suggesting that all three types of motif may be involved in GLUT4 trafficking. Piper et al. [19] have shown that GLUT1}GLUT4 and asialoglycopro ...
... case of TGN38, both a transmembrane domain and a β-turn type of motif [17,18]. GLUT4 has several potential targeting domains and evidence has been presented suggesting that all three types of motif may be involved in GLUT4 trafficking. Piper et al. [19] have shown that GLUT1}GLUT4 and asialoglycopro ...
articles - Saleh Lab
... but seem to be unable to transmit this dsRNA to other cells in the body4. dsRNA uptake without further transmission to other cells has also been reported for some mammalian cell types5–7. Other organisms (such as C. elegans or juvenile grasshoppers) can both take up dsRNA and spread it systemically ...
... but seem to be unable to transmit this dsRNA to other cells in the body4. dsRNA uptake without further transmission to other cells has also been reported for some mammalian cell types5–7. Other organisms (such as C. elegans or juvenile grasshoppers) can both take up dsRNA and spread it systemically ...
Signal transduction
Signal transduction occurs when an extracellular signaling molecule activates a specific receptor located on the cell surface or inside the cell. In turn, this receptor triggers a biochemical chain of events inside the cell, creating a response. Depending on the cell, the response alters the cell's metabolism, shape, gene expression, or ability to divide. The signal can be amplified at any step. Thus, one signaling molecule can cause many responses.