PLC Biology
... Cystic fibrosis Embryo screening B2.8 Speciation Fossils and their formation Causes of extinction Isolation and new species ...
... Cystic fibrosis Embryo screening B2.8 Speciation Fossils and their formation Causes of extinction Isolation and new species ...
Remodeling of endosomes during lysosome biogenesis involves
... stable cell lines (Duclos et al., 2000) and selected clones expressing low levels of the protein as measured by western blot analysis using an anti-rab5 antibody (not shown). These cells were loaded with the fluid phase marker HRP for 30 minutes in order to visualize the morphology of their endocyti ...
... stable cell lines (Duclos et al., 2000) and selected clones expressing low levels of the protein as measured by western blot analysis using an anti-rab5 antibody (not shown). These cells were loaded with the fluid phase marker HRP for 30 minutes in order to visualize the morphology of their endocyti ...
Automatic sampling for unbiased and efficient stereological
... The proportionator is compared with the traditional SURS using the aforementioned three biological examples, using a microscope system modified for stereology (detailed setup is presented in appendix A). In all examples, four independent estimates are obtained for each slide: two estimates using the ...
... The proportionator is compared with the traditional SURS using the aforementioned three biological examples, using a microscope system modified for stereology (detailed setup is presented in appendix A). In all examples, four independent estimates are obtained for each slide: two estimates using the ...
Ultrasound-triggered disruption and self-healing of reversibly cross-linked hydrogels for drug delivery
... to treat breast cancer, is a charged molecule that forms an ionic complex with alginate (21) and thus exhibited a low baseline level of release from alginate gels in the absence of ultrasound (Fig. 2 A and C). One of the drawbacks of mitoxantrone clinically is its off-target toxicity, which may be m ...
... to treat breast cancer, is a charged molecule that forms an ionic complex with alginate (21) and thus exhibited a low baseline level of release from alginate gels in the absence of ultrasound (Fig. 2 A and C). One of the drawbacks of mitoxantrone clinically is its off-target toxicity, which may be m ...
Inhibition of c-myc Expression in Cells by Targeting an RNA
... it may not always be the best site. For some mRNAs, sites in the 5⬘- untranslated, protein coding, and 3⬘-untranslated regions have been shown to be more sensitive to ODN action than the translation initiation codon (Bacon and Wickstrom, 1991; Chiang et al., 1991; Crooke, 1993; Gewirtz et al., 1998) ...
... it may not always be the best site. For some mRNAs, sites in the 5⬘- untranslated, protein coding, and 3⬘-untranslated regions have been shown to be more sensitive to ODN action than the translation initiation codon (Bacon and Wickstrom, 1991; Chiang et al., 1991; Crooke, 1993; Gewirtz et al., 1998) ...
Structure and function of basement membranes
... Several additional subunits of the laminin family have been discovered from cDNA cloning or characterization of extracellular matrix from tissues or cultured cells. In our search for tissue-specific basement membrane components, we discovered the a2 chain, which is a homolog of the classical al chai ...
... Several additional subunits of the laminin family have been discovered from cDNA cloning or characterization of extracellular matrix from tissues or cultured cells. In our search for tissue-specific basement membrane components, we discovered the a2 chain, which is a homolog of the classical al chai ...
Leaf Development Outline Leaf Structure
... Two Æ Apical-Basal axis = tip to base. Three Æ Lateral axis = width SEM of young wheat stem ...
... Two Æ Apical-Basal axis = tip to base. Three Æ Lateral axis = width SEM of young wheat stem ...
Antisense therapy in oncology: new hope for an old idea?
... with survival of stage IV malignant melanoma patients (usually 6–9 months with and without treatment). This study is worth mentioning because it was the first antisense trial in which downregulation of the target protein in the target tissue was shown. Based on the promising results of this study, t ...
... with survival of stage IV malignant melanoma patients (usually 6–9 months with and without treatment). This study is worth mentioning because it was the first antisense trial in which downregulation of the target protein in the target tissue was shown. Based on the promising results of this study, t ...
Microenvironmental reprogramming by three
... just to contribute to hair follicles within the skin, but rather, to fully reprogram the recipient epidermis to a follicular fate. Intact dermal papillae were microdissected from adult human hair follicles and placed between the epidermis and dermis of dispase-separated foreskin tissue. On two occas ...
... just to contribute to hair follicles within the skin, but rather, to fully reprogram the recipient epidermis to a follicular fate. Intact dermal papillae were microdissected from adult human hair follicles and placed between the epidermis and dermis of dispase-separated foreskin tissue. On two occas ...
The cell biology of hearing - The Journal of Cell Biology
... a nanometer, can amplify signals by more than 100-fold, and has a wide dynamic range enabling humans to perceive frequencies from 20 Hz to 20 kHz. Essential for this extraordinary capability are the mechanosensory hair cells, which together with supporting cells and accessory extracellular structure ...
... a nanometer, can amplify signals by more than 100-fold, and has a wide dynamic range enabling humans to perceive frequencies from 20 Hz to 20 kHz. Essential for this extraordinary capability are the mechanosensory hair cells, which together with supporting cells and accessory extracellular structure ...
Harvard-MIT Division Of Health Sciences And Technology HST
... chip and to each one of the microspheres. Optical signals generated by the reactions on the microspheres are visualized and captured on a charge-coupled device (CCD) with the use of transfer optics and a digital video chip. Using the ETC system, complex immunological assays, such as the ones develop ...
... chip and to each one of the microspheres. Optical signals generated by the reactions on the microspheres are visualized and captured on a charge-coupled device (CCD) with the use of transfer optics and a digital video chip. Using the ETC system, complex immunological assays, such as the ones develop ...
EFFECT OF ASCORBIC ACID CONCENTRATION ON
... squeezed and more arranged than that of control samples, (4) Differentiation of central column in plants treated with ascorbic acid occurred sooner and generated earlier than that of control plants, (5) Compared to the control samples, quiescent center of roots in samples treated with ascorbic acid ...
... squeezed and more arranged than that of control samples, (4) Differentiation of central column in plants treated with ascorbic acid occurred sooner and generated earlier than that of control plants, (5) Compared to the control samples, quiescent center of roots in samples treated with ascorbic acid ...
Structural and functional classes of multipolar cells in the ventral
... neurons in mice (Oertel et al., 1990). In contrast, the parent axons of D-stellate neurons project dorsally beneath the dorsal cochlear nucleus (DCN; Fig. 1B). Most of our knowledge pertaining to the neural mechanisms that govern the responses of multipolar neurons derives from in vitro descriptions ...
... neurons in mice (Oertel et al., 1990). In contrast, the parent axons of D-stellate neurons project dorsally beneath the dorsal cochlear nucleus (DCN; Fig. 1B). Most of our knowledge pertaining to the neural mechanisms that govern the responses of multipolar neurons derives from in vitro descriptions ...
t-SNARE Phosphorylation Regulates Endocytosis in Yeast
... with active ceramide analogs, by VBM gene inactivation, or by overexpression of SIT4 itself. Interestingly, endocytic defects in cells lacking either Tlg1 or Tlg2 alone or in combination with deletions in the SNC genes are also rescued by CAPP activation. In all cases, rescue correlated with the dep ...
... with active ceramide analogs, by VBM gene inactivation, or by overexpression of SIT4 itself. Interestingly, endocytic defects in cells lacking either Tlg1 or Tlg2 alone or in combination with deletions in the SNC genes are also rescued by CAPP activation. In all cases, rescue correlated with the dep ...
THERMOSENSITIVE HYDROGEL FOR CONTROLLED DRUG DELIVERY OF ANTICANCER AGENTS Research Article
... delivery of camptothecin in a slow release manner directly into a tumor cell. In this paper, we report the in vitro release characteristics of the camptothecin-polymer hydrogel and the in vitro effect of delivering camptothecin in different concentrations to a MFC7 tumor cell. The delivery vehicle u ...
... delivery of camptothecin in a slow release manner directly into a tumor cell. In this paper, we report the in vitro release characteristics of the camptothecin-polymer hydrogel and the in vitro effect of delivering camptothecin in different concentrations to a MFC7 tumor cell. The delivery vehicle u ...
Determinants of Drosophila zw10 protein localization and function
... text) unassociated with nuclei (arrowheads in C). These free asters may in turn result in the formation of tripolar spindles when they become attached to nearby nuclei (outline arrow, C and D). The uneven spacing of nuclei can lead to spindle fusions, since a single pole is shared by two spindles (l ...
... text) unassociated with nuclei (arrowheads in C). These free asters may in turn result in the formation of tripolar spindles when they become attached to nearby nuclei (outline arrow, C and D). The uneven spacing of nuclei can lead to spindle fusions, since a single pole is shared by two spindles (l ...
Human pancreas development - The Company of Biologists
... 8 weeks of development prior to the fetal period. Counting somite pairs by light microscopy becomes more difficult after CS11. AIP, anterior intestinal portal. *The estimation of mouse development relates broadly to the Carnegie stage, not the key event in human pancreas development. ...
... 8 weeks of development prior to the fetal period. Counting somite pairs by light microscopy becomes more difficult after CS11. AIP, anterior intestinal portal. *The estimation of mouse development relates broadly to the Carnegie stage, not the key event in human pancreas development. ...
Lysosomal enzymes and inflammation
... which is studied by these cytochemical and biochemical procedures. Secondary lysosomes can also be formed by autophagic processes, in which parts of the cytoplasm, including mitochondria, are taken up into a digestive vacuole which fuses with primary lysosomes. This process of autophagy (Novikoff, 1 ...
... which is studied by these cytochemical and biochemical procedures. Secondary lysosomes can also be formed by autophagic processes, in which parts of the cytoplasm, including mitochondria, are taken up into a digestive vacuole which fuses with primary lysosomes. This process of autophagy (Novikoff, 1 ...
Heat shock results in cell cycle delay and synchronisation of mitotic
... this study, embryos were selected at the three indicated morphological stages: embryos at the early stages of cellularisation were taken to be in ‘S-phase’; embryos showing the early gastrulation movements of cephalic furrow formation are designated ‘early G2-phase’; and embryos in which gastrulatio ...
... this study, embryos were selected at the three indicated morphological stages: embryos at the early stages of cellularisation were taken to be in ‘S-phase’; embryos showing the early gastrulation movements of cephalic furrow formation are designated ‘early G2-phase’; and embryos in which gastrulatio ...
FEMS Microbiology Ecology
... abundance of the isolates in situ was known. The MPN technique for rhizoplane specimens was described previously [8]. Methanotrophs were grown in nitrate mineral salts medium containing (in grams per liter) KNO3 , 1.0; KH2 PO4 , 0.54; Mg2 SO4 W7H2 O, 0.2; CaCl2 W2 H2 O, 0.015 (Mg2 SO4 and CaCl2 were ...
... abundance of the isolates in situ was known. The MPN technique for rhizoplane specimens was described previously [8]. Methanotrophs were grown in nitrate mineral salts medium containing (in grams per liter) KNO3 , 1.0; KH2 PO4 , 0.54; Mg2 SO4 W7H2 O, 0.2; CaCl2 W2 H2 O, 0.015 (Mg2 SO4 and CaCl2 were ...
14 March 2017 ABI Response to FCA CP17/3: Proposed Handbook
... necessary for firms to submit a separate notification to the FCA at the same time as the PRA as the same time-restraints will not apply. A single, post-launch notification form to the lead regulator (the PRA) would be therefore be preferable. Proposal to make ISPVs subject to requirements of SYSC 3, ...
... necessary for firms to submit a separate notification to the FCA at the same time as the PRA as the same time-restraints will not apply. A single, post-launch notification form to the lead regulator (the PRA) would be therefore be preferable. Proposal to make ISPVs subject to requirements of SYSC 3, ...
Rat embryonic ectoderm as renal isograft - Development
... the host. These are: (a) interruption of the supply of oxygen and nutrients by uterine blood circulation, (b) influence of new environmental constituents (saline, serum, enzymes), (c) surgical trauma, (d) loss of connection with extraembryonic membranes and of contact with extraembryonic fluids, (e) ...
... the host. These are: (a) interruption of the supply of oxygen and nutrients by uterine blood circulation, (b) influence of new environmental constituents (saline, serum, enzymes), (c) surgical trauma, (d) loss of connection with extraembryonic membranes and of contact with extraembryonic fluids, (e) ...
FVIII-specific human chimeric antigen receptor T
... experiment was performed with transduced T cells generated from a single donor and repeated .3 times with PBMCs from different donors with similar results. Flow cytometry analysis and antibodies Isolated cells were washed (3% FBS in phenol-red free RPMI) and stained with fluorochrome-conjugated antib ...
... experiment was performed with transduced T cells generated from a single donor and repeated .3 times with PBMCs from different donors with similar results. Flow cytometry analysis and antibodies Isolated cells were washed (3% FBS in phenol-red free RPMI) and stained with fluorochrome-conjugated antib ...
The zebrafish midblastula transition - Development
... indicating possible MBT-like cell cycle lengthening. In studies on the loach, Misgurnus fossilis, Rott and Shevelava (1967) found a more defined cell cycle lengthening, which did not occur until cycle 11, and, in the same study, they found the equivalent cell cycle lengthening in haploid embryos occ ...
... indicating possible MBT-like cell cycle lengthening. In studies on the loach, Misgurnus fossilis, Rott and Shevelava (1967) found a more defined cell cycle lengthening, which did not occur until cycle 11, and, in the same study, they found the equivalent cell cycle lengthening in haploid embryos occ ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.