Construction and applications of yellow fever virus replicons
... laboratory manipulations of many of these viruses remain difficult due to their highly pathogenic properties. An alternative approach to studying infectious, potentially dangerous, flaviviruses has been described for several flaviviruses, including WNV, DEN, and TBE, and involves the construction of ...
... laboratory manipulations of many of these viruses remain difficult due to their highly pathogenic properties. An alternative approach to studying infectious, potentially dangerous, flaviviruses has been described for several flaviviruses, including WNV, DEN, and TBE, and involves the construction of ...
PHAGOLYSOSOME FORMATION IN NORMAL AND
... It has been suggested that intact microtubules promote contact of lysosomes with endosomes in phagocytic cells and that microtubular disruption caused by colchicine (1-6) or vinblastine (7) would inhibit fusion of lysosomes with phagosomes or pinosomes . We have previously shown (8) that in the macr ...
... It has been suggested that intact microtubules promote contact of lysosomes with endosomes in phagocytic cells and that microtubular disruption caused by colchicine (1-6) or vinblastine (7) would inhibit fusion of lysosomes with phagosomes or pinosomes . We have previously shown (8) that in the macr ...
Biology - Prescott Unified School District
... Explain how the two groups of prokaryotes differ. Describe the factors that are used to identify prokaryotes. Explain why bacteria are vital to maintaining the living world. Analyze author’s purpose in discussing an experiment in text, defining the question the author seeks to address (Bact Lab) ...
... Explain how the two groups of prokaryotes differ. Describe the factors that are used to identify prokaryotes. Explain why bacteria are vital to maintaining the living world. Analyze author’s purpose in discussing an experiment in text, defining the question the author seeks to address (Bact Lab) ...
Ultrastructure of the Pulmonary Alveolar Cells of Rats Exposed to
... tissue. Cigarette smoke is the most known hazard and its association with pulmonary diseases was extensively studied. However, another common activity which poses threats to the human health is the incense burning which is usually overlooked[2]. It is a ceremonial practice in countries of East-South ...
... tissue. Cigarette smoke is the most known hazard and its association with pulmonary diseases was extensively studied. However, another common activity which poses threats to the human health is the incense burning which is usually overlooked[2]. It is a ceremonial practice in countries of East-South ...
Organization of the Sea Urchin Egg Endoplasmic Reticulum and Its
... results in a rearrangement of the subcortical ER near the animal pole (Campanella et al., 1988) . Using the DiI-oil drop injection technique, we have now made continuous observations of the rearrangements of the ER in the living sea urchin egg during fertilization . We find that within the first 20 ...
... results in a rearrangement of the subcortical ER near the animal pole (Campanella et al., 1988) . Using the DiI-oil drop injection technique, we have now made continuous observations of the rearrangements of the ER in the living sea urchin egg during fertilization . We find that within the first 20 ...
Homogeneous generation of iDA neurons with high similarity to
... that abundant and readily accessible somatic cell types, such as peripheral blood cells, are amenable to direct lineage reprogramming and can give rise to functional iDA neurons. Taken together, our results suggest that inducible gene expression in a genetically homogeneous system enables the efficie ...
... that abundant and readily accessible somatic cell types, such as peripheral blood cells, are amenable to direct lineage reprogramming and can give rise to functional iDA neurons. Taken together, our results suggest that inducible gene expression in a genetically homogeneous system enables the efficie ...
Microtubules Regulate Dynamic Organization of Vacuoles in
... with reconstruction of their three-dimensional structures has revealed the complexity of these structures (Yoneda et al. 2007). For instance, spherical membranes and sheet-like membranes have been reported within the vacuolar lumen of Arabidopsis epidermal cells (Saito et al. 2002), and dynamic chan ...
... with reconstruction of their three-dimensional structures has revealed the complexity of these structures (Yoneda et al. 2007). For instance, spherical membranes and sheet-like membranes have been reported within the vacuolar lumen of Arabidopsis epidermal cells (Saito et al. 2002), and dynamic chan ...
as a PDF
... fusion proteins (n = 33 cells, 4.1min average duration at 3.85sec intervals) revealed discrete sites of apparent microtubule initiation at the cell cortex (Fig. 1A,B). Microtubules that depolymerized to visible completion rarely showed recovery (48 of 50 events with >2min of imaging after depolymeri ...
... fusion proteins (n = 33 cells, 4.1min average duration at 3.85sec intervals) revealed discrete sites of apparent microtubule initiation at the cell cortex (Fig. 1A,B). Microtubules that depolymerized to visible completion rarely showed recovery (48 of 50 events with >2min of imaging after depolymeri ...
The Use of Mucoadhesive Polymers to Enhance the Hypotensive
... unusual to find therapies that combine two or more drugs, which can often lead to reduced patient compliance.2– 4 Allergic reactions and adverse effects associated with chronic glaucoma treatment have been described.5,6 These secondary effects are usually related to the drug or other components pres ...
... unusual to find therapies that combine two or more drugs, which can often lead to reduced patient compliance.2– 4 Allergic reactions and adverse effects associated with chronic glaucoma treatment have been described.5,6 These secondary effects are usually related to the drug or other components pres ...
Rac1 accumulates in the nucleus during the G2 phase of the cell
... the Rho family of small GTPases. Like all GTPases, Rac1 functions as a molecular switch regulated by GTP/GDP exchange. Rac1 regulates a wide variety of cellular functions including actin remodeling for cell ruffling, adherens junction formation, cell motility, and polarity. Other functions of Rac1 i ...
... the Rho family of small GTPases. Like all GTPases, Rac1 functions as a molecular switch regulated by GTP/GDP exchange. Rac1 regulates a wide variety of cellular functions including actin remodeling for cell ruffling, adherens junction formation, cell motility, and polarity. Other functions of Rac1 i ...
Phosphorylation-Dependent Regulation of Septin
... that the cdc12-6 septin allele leads to septin ring disassembly upon shift to the restrictive temperature (Barral et al., 2000). We rationalized that changes in the rate of disassembly at specific cell cycle stages would reflect changes in septin dissociation/association rates. GFPCDC3 cdc12-6 cells ...
... that the cdc12-6 septin allele leads to septin ring disassembly upon shift to the restrictive temperature (Barral et al., 2000). We rationalized that changes in the rate of disassembly at specific cell cycle stages would reflect changes in septin dissociation/association rates. GFPCDC3 cdc12-6 cells ...
Function Antigens but with Altered Cytokine Secretion Cells To
... “signal 3” cytokines (e.g., IL-12). Mature DCs efficiently present Ags linked to the activating PAMP and prime naive T cells. However, mature DCs downregulate MHC II synthesis, which prevents them from presenting newly encountered Ags. DCs can also be indirectly activated by inflammatory mediators r ...
... “signal 3” cytokines (e.g., IL-12). Mature DCs efficiently present Ags linked to the activating PAMP and prime naive T cells. However, mature DCs downregulate MHC II synthesis, which prevents them from presenting newly encountered Ags. DCs can also be indirectly activated by inflammatory mediators r ...
Analysis of the paralysed trypanosome mutant snl-1
... posterior end of the cell. The flagellum is attached throughout most of its length to a region of the cell body termed the flagellum attachment zone (Kohl and Gull, 1998). The basal body of the flagellum is tightly connected to the kinetoplast, the condensed DNA of the single mitochondrion (Robinson ...
... posterior end of the cell. The flagellum is attached throughout most of its length to a region of the cell body termed the flagellum attachment zone (Kohl and Gull, 1998). The basal body of the flagellum is tightly connected to the kinetoplast, the condensed DNA of the single mitochondrion (Robinson ...
Specific vasopressin binding to rat adrenal glomerulosa cells
... To test the stability of [3H]vasopressin during the course of the binding assay, glomerulosa cells were incubated for 15 min in the presence of [3H]vasopressin (5 nM). A small sample of the culture medium was collected at the end of the incubation period and submitted to h.p.l.c. analysis. The eluti ...
... To test the stability of [3H]vasopressin during the course of the binding assay, glomerulosa cells were incubated for 15 min in the presence of [3H]vasopressin (5 nM). A small sample of the culture medium was collected at the end of the incubation period and submitted to h.p.l.c. analysis. The eluti ...
Penetration and Establishment of Phakopsora pachyrhizi in
... effective diagnostic techniques (1,9). A detailed knowledge of how the fungus infects the host could be useful in directing research for effective controls based on disease resistance. Biotrophic fungal pathogens use several different strategies to infect a host. Fungi, such as the apple scab pathog ...
... effective diagnostic techniques (1,9). A detailed knowledge of how the fungus infects the host could be useful in directing research for effective controls based on disease resistance. Biotrophic fungal pathogens use several different strategies to infect a host. Fungi, such as the apple scab pathog ...
Metabolomics of a single vacuole reveals metabolic dynamism in an
... distribution of phosphate compounds has not yet been confirmed. Although subcellular metabolite distribution has been measured by various techniques, non-aqueous fractionation is one of the most promising approaches for studying metabolite compartmentalization (Wirtz et al., 1980; Gerhardt and Heldt ...
... distribution of phosphate compounds has not yet been confirmed. Although subcellular metabolite distribution has been measured by various techniques, non-aqueous fractionation is one of the most promising approaches for studying metabolite compartmentalization (Wirtz et al., 1980; Gerhardt and Heldt ...
Direct inhibition of CD40L expression can contribute
... Adult PBMCs contain both naive (CD45RA⫹CD45RO⫺) and memory (CD45RA⫺CD45RO⫹) CD4⫹ T cells. Memory cells are generally considered to have a lower threshold for activation, possibly diminishing or negating their need for a costimulatory signal. We thus hypothesized that CD28-independent CD40L at 6 hour ...
... Adult PBMCs contain both naive (CD45RA⫹CD45RO⫺) and memory (CD45RA⫺CD45RO⫹) CD4⫹ T cells. Memory cells are generally considered to have a lower threshold for activation, possibly diminishing or negating their need for a costimulatory signal. We thus hypothesized that CD28-independent CD40L at 6 hour ...
Effects of intestinal adaptation on insulin binding to villus cell
... which is normally induced by fasting.'9 These effects of insulin would suggest that the insulin binding sites present on villus cells are of physiological significance in that they promote or facilitate differentiation of the cell. Interestingly, insulin does not stimulate amino acid absorption by i ...
... which is normally induced by fasting.'9 These effects of insulin would suggest that the insulin binding sites present on villus cells are of physiological significance in that they promote or facilitate differentiation of the cell. Interestingly, insulin does not stimulate amino acid absorption by i ...
The Lymphoid System - Napa Valley College
... • Memory T cells (become activated if the same antigen appears in the body at a later date) © 2012 Pearson Education, Inc. ...
... • Memory T cells (become activated if the same antigen appears in the body at a later date) © 2012 Pearson Education, Inc. ...
Regular-spiking cells in the presubiculum are hyperexcitable in a rat
... capable of evoking epileptiform activity specific to this region in epileptic animals (Tolner et al. 2005, 2007). Recently, we characterized PrS neurons into distinct groups using hierarchical cluster analysis based on electrophysiological criteria, establishing baselines for intrinsic properties me ...
... capable of evoking epileptiform activity specific to this region in epileptic animals (Tolner et al. 2005, 2007). Recently, we characterized PrS neurons into distinct groups using hierarchical cluster analysis based on electrophysiological criteria, establishing baselines for intrinsic properties me ...
PLC Biology
... Cystic fibrosis Embryo screening B2.8 Speciation Fossils and their formation Causes of extinction Isolation and new species ...
... Cystic fibrosis Embryo screening B2.8 Speciation Fossils and their formation Causes of extinction Isolation and new species ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.