Review Recycling the Cell Cycle: Cyclins Revisited
... Before such a feature could appear, a tighter coupling between replication and segregation was necessary. One form of coupling would be a clock that told previously independent processes when to begin, coupled with long intervals between the striking of the hours so that one event would finish befor ...
... Before such a feature could appear, a tighter coupling between replication and segregation was necessary. One form of coupling would be a clock that told previously independent processes when to begin, coupled with long intervals between the striking of the hours so that one event would finish befor ...
Inhibition of Target of Rapamycin Signaling and
... The unicellular green alga Chlamydomonas reinhardtii has been used as a model for the study of important cellular and metabolic processes in photosynthetic organisms (Harris, 2001). More recently, Chlamydomonas has also been proposed as a useful system for the characterization of the TOR signaling p ...
... The unicellular green alga Chlamydomonas reinhardtii has been used as a model for the study of important cellular and metabolic processes in photosynthetic organisms (Harris, 2001). More recently, Chlamydomonas has also been proposed as a useful system for the characterization of the TOR signaling p ...
Presence of methyl sterol and bacteriohopanepolyol
... Dyer (1959) extraction as modified by Kates (1986). The residue was recovered by centrifugation before initiating phase separation and was used for P-hydroxy fatty acid analysis (see below). The total lipid extract was analysed for phospholipid phosphate (Dittmer & Wells, 1969), and phospholipid est ...
... Dyer (1959) extraction as modified by Kates (1986). The residue was recovered by centrifugation before initiating phase separation and was used for P-hydroxy fatty acid analysis (see below). The total lipid extract was analysed for phospholipid phosphate (Dittmer & Wells, 1969), and phospholipid est ...
The Regulation of S Phase Initiation by p27Kip1 in NIH3T3 Cells
... with 3% paraformaldehyde/0.05% Triton X100 and then with chilled methanol for 2 min (p27), with chilled methanol alone (cyclin D1 and Skp2) and stained with an indirect antibody stain against the target antigen, and finally stained with DAPI (10 µg/ml). Images of each fluorochrome were taken with a ...
... with 3% paraformaldehyde/0.05% Triton X100 and then with chilled methanol for 2 min (p27), with chilled methanol alone (cyclin D1 and Skp2) and stained with an indirect antibody stain against the target antigen, and finally stained with DAPI (10 µg/ml). Images of each fluorochrome were taken with a ...
When cells get stressed: an integrative view of cellular
... doing so within a period as short as several days. Such stresses include DNA-damaging agents, oxidative stress, “oncogenic stress” (due to oncogene overexpression), and other metabolic perturbations (5, 18–22). Typically, these forms of senescence do not involve significant telomere shortening and c ...
... doing so within a period as short as several days. Such stresses include DNA-damaging agents, oxidative stress, “oncogenic stress” (due to oncogene overexpression), and other metabolic perturbations (5, 18–22). Typically, these forms of senescence do not involve significant telomere shortening and c ...
When cells get stressed: an integrative view of cellular senescence
... doing so within a period as short as several days. Such stresses include DNA-damaging agents, oxidative stress, “oncogenic stress” (due to oncogene overexpression), and other metabolic perturbations (5, 18–22). Typically, these forms of senescence do not involve significant telomere shortening and c ...
... doing so within a period as short as several days. Such stresses include DNA-damaging agents, oxidative stress, “oncogenic stress” (due to oncogene overexpression), and other metabolic perturbations (5, 18–22). Typically, these forms of senescence do not involve significant telomere shortening and c ...
Chromosomes with Two Intact Axial Cores Are Induced by G2
... dent duplexes to form two chromatid arms, each with an axial core. Alternatively, it is possible that the information necessary to form the two chromatids with axial cores has been templated independently, and before, the decatenation event. Here we have approached the problem of the interdependence ...
... dent duplexes to form two chromatid arms, each with an axial core. Alternatively, it is possible that the information necessary to form the two chromatids with axial cores has been templated independently, and before, the decatenation event. Here we have approached the problem of the interdependence ...
The essential role of anionic transport in plant cells
... endoplasmic reticulum, mitochondria, and chloroplasts (reviewed in Barbier-Brygoo et al., 2000; Roberts, 2006; de Angeli et al., 2007). The presence of the wall has hindered electrophysiological studies, which require free access to the plasma membrane. The use of cell protoplasts, obtained by eithe ...
... endoplasmic reticulum, mitochondria, and chloroplasts (reviewed in Barbier-Brygoo et al., 2000; Roberts, 2006; de Angeli et al., 2007). The presence of the wall has hindered electrophysiological studies, which require free access to the plasma membrane. The use of cell protoplasts, obtained by eithe ...
From signal to form: aspects of the cytoskeleton
... made with molecular genetics that will allow the unravelling of the signal transduction pathways in root hair morphogenesis leading to growth. This paper first discusses cytological phenomena involved in the process of polar growth, such as cytoplasmic polarity, cytoplasmic streaming and the organiz ...
... made with molecular genetics that will allow the unravelling of the signal transduction pathways in root hair morphogenesis leading to growth. This paper first discusses cytological phenomena involved in the process of polar growth, such as cytoplasmic polarity, cytoplasmic streaming and the organiz ...
Coca Cola
... A polarized cell: stabilized MTs serve as rail tracks that transport membrane vesicles and actin regulatory proteins to MT (+) ends ...
... A polarized cell: stabilized MTs serve as rail tracks that transport membrane vesicles and actin regulatory proteins to MT (+) ends ...
Characterization of Human Corneal Epithelial Cell Model As a
... Transcorneal Permeability in Excised Rabbits Corneas and cHCE. The permeability of eight ophthalmic drugs and three -blockers (atenolol, metoprolol, and nadolol) was assessed using either excised rabbit corneas or cHCE. The Papp values for the 11 compounds track well between values for the two mode ...
... Transcorneal Permeability in Excised Rabbits Corneas and cHCE. The permeability of eight ophthalmic drugs and three -blockers (atenolol, metoprolol, and nadolol) was assessed using either excised rabbit corneas or cHCE. The Papp values for the 11 compounds track well between values for the two mode ...
Emerging regulatory mechanisms in ubiquitin
... Accurate cell division is no trivial task: cells need to duplicate their genomic material, correct mistakes made by sloppy DNA polymerases, repair damage caused by harsh environments and yet still distribute their chromosomes into identical daughter cells. Errors in this program can be deadly for th ...
... Accurate cell division is no trivial task: cells need to duplicate their genomic material, correct mistakes made by sloppy DNA polymerases, repair damage caused by harsh environments and yet still distribute their chromosomes into identical daughter cells. Errors in this program can be deadly for th ...
- RichardWheeler.net
... Three-dimensional electron microscopy tools have revolutionized our understanding of cell structure and molecular complexes in biology. Here, we describe methods for studying flagellar ultrastructure and biogenesis in two unicellular parasitesdTrypanosoma brucei and Leishmania mexicana. We describe ...
... Three-dimensional electron microscopy tools have revolutionized our understanding of cell structure and molecular complexes in biology. Here, we describe methods for studying flagellar ultrastructure and biogenesis in two unicellular parasitesdTrypanosoma brucei and Leishmania mexicana. We describe ...
siRNA back - Santa Cruz Biotechnology
... (Fluorescein Conjugates): sc-36869, sc-44239, sc-44240 or sc-44241. Each contain a scrambled sequence that will not lead to the specific degradation of any known cellular mRNA. ...
... (Fluorescein Conjugates): sc-36869, sc-44239, sc-44240 or sc-44241. Each contain a scrambled sequence that will not lead to the specific degradation of any known cellular mRNA. ...
TMBP200, a Microtubule Bundling Polypeptide Isolated from
... and in glycerinated tobacco BY-2 cells (Asada et al. 1991). In glycerinated tobacco BY-2 cells, microtubules polymerized at the equatorial region were translocated away from the equatorial region in an ATP-dependent manner and the motor molecule responsible for such microtubule translocation has bee ...
... and in glycerinated tobacco BY-2 cells (Asada et al. 1991). In glycerinated tobacco BY-2 cells, microtubules polymerized at the equatorial region were translocated away from the equatorial region in an ATP-dependent manner and the motor molecule responsible for such microtubule translocation has bee ...
Computational optogenetics: A novel continuum framework for the
... pericardial effusion, cardiac tamponade, pneumothorax, and death (Mahapatra et al., 2005). However, to date, there is no conceptual alternative to electrical pacing that avoids direct contact stimulus delivery (Khan et al., 2005). For more than 40 years, biologists have studied microorganisms that p ...
... pericardial effusion, cardiac tamponade, pneumothorax, and death (Mahapatra et al., 2005). However, to date, there is no conceptual alternative to electrical pacing that avoids direct contact stimulus delivery (Khan et al., 2005). For more than 40 years, biologists have studied microorganisms that p ...
development through auxin-induced cytokinin oxidase activity
... treatment (Fig. 2A, insets). To assess the effects of low R/FR on auxin signaling in leaf primordia at a later developmental stage, DR5⬋GUS seedlings were grown for 6 d in high R/FR, and then maintained in high R/FR or transferred to low R/FR for 4 h. At this stage, the initiation of the leaf lamina ...
... treatment (Fig. 2A, insets). To assess the effects of low R/FR on auxin signaling in leaf primordia at a later developmental stage, DR5⬋GUS seedlings were grown for 6 d in high R/FR, and then maintained in high R/FR or transferred to low R/FR for 4 h. At this stage, the initiation of the leaf lamina ...
Toll-like receptor 4–dependent contribution of the immune system to
... Toll-like receptors (TLRs) recognize molecules derived from pathogens as well as endogenous danger signals possessing similar chemical structures9,10. Upon recognition of their ligands, TLRs transduce signals through two pathways involving distinct adaptors, Toll/IL-1R domain–containing adaptor indu ...
... Toll-like receptors (TLRs) recognize molecules derived from pathogens as well as endogenous danger signals possessing similar chemical structures9,10. Upon recognition of their ligands, TLRs transduce signals through two pathways involving distinct adaptors, Toll/IL-1R domain–containing adaptor indu ...
Circulating Tumor Cells: A Window Into Tumor Development and
... Most human solid tumors contain an heterogeneous population of cancer cells that originate from epithelial cell types; many of these are shed on a daily basis into the blood, where they survive a couple of hours.54 Some are apoptotic,55,56 whereas others are destroyed by the immune system,57 even th ...
... Most human solid tumors contain an heterogeneous population of cancer cells that originate from epithelial cell types; many of these are shed on a daily basis into the blood, where they survive a couple of hours.54 Some are apoptotic,55,56 whereas others are destroyed by the immune system,57 even th ...
Podosome-type adhesions and focal adhesions, so alike yet so
... sites so close in their composition can be structurally and functionally so different. The underlying question is how their respective physiological or pathological roles are related to their distinct organization. ...
... sites so close in their composition can be structurally and functionally so different. The underlying question is how their respective physiological or pathological roles are related to their distinct organization. ...
MEMBRANE PROTEINS SYNTHESIZED BY
... The proteins present in partially purified membrane preparations from human erythrocytes, rabbit reticulocytes, and rabbit erythrocytes were resolved by electrophoresis in polyacrylamide gels containing urea and SDS, and visualized by staining with Coomassie blue (Fig. 3 a, b, e). The numbering of t ...
... The proteins present in partially purified membrane preparations from human erythrocytes, rabbit reticulocytes, and rabbit erythrocytes were resolved by electrophoresis in polyacrylamide gels containing urea and SDS, and visualized by staining with Coomassie blue (Fig. 3 a, b, e). The numbering of t ...
Title Soybean extracts increase cell surface ZIP4 abundance and
... would enable the increase of both cell surface ZIP4 expression and cellular zinc levels. This technique identified soybean extracts as containing such components, which included soyasaponin Bb. Soyasaponin Bb increased the cell surface expression of both mouse Zip4 (mZip4) and human ZIP4 (hZIP4), as ...
... would enable the increase of both cell surface ZIP4 expression and cellular zinc levels. This technique identified soybean extracts as containing such components, which included soyasaponin Bb. Soyasaponin Bb increased the cell surface expression of both mouse Zip4 (mZip4) and human ZIP4 (hZIP4), as ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.