Decontamination of the BD FACSAria System
... of antibiotic-resistant bacterial contamination. The presence of these antibioticresistant bacteria might result in a depletion of essential nutrients and factors required by the cells in culture and the accumulation of bacterial metabolic waste products. These waste products might have unknown, unw ...
... of antibiotic-resistant bacterial contamination. The presence of these antibioticresistant bacteria might result in a depletion of essential nutrients and factors required by the cells in culture and the accumulation of bacterial metabolic waste products. These waste products might have unknown, unw ...
Targeting of Active Sialyltransferase to the Plant Golgi Apparatus
... Callus lines were induced from the transgenic plants and maintained in liquid culture. Transit of ST through the secretory pathway in the callus cells was investigated by endo-b-N-acetylglucosaminidase H (Endo H) digestion of proteins. This enzyme cleaves the chitobiose core of high-mannose and hybr ...
... Callus lines were induced from the transgenic plants and maintained in liquid culture. Transit of ST through the secretory pathway in the callus cells was investigated by endo-b-N-acetylglucosaminidase H (Endo H) digestion of proteins. This enzyme cleaves the chitobiose core of high-mannose and hybr ...
Early Development and Axis Formation in Amphibians
... & intermediate - mesenchyme, blood muscle, kidney ...
... & intermediate - mesenchyme, blood muscle, kidney ...
Amino acid sequence identity between the HA1 of influenza A
... HA and neuraminidase of relevant epidemic strains (Maassab et al., 1985). Such viruses are under trial as live attenuated influenza vaccines (Wright et al., 1982; Murphy & Clements, 1989). Because potential reference and vaccine strains of virus may also be initially isolated in mammalian cells of d ...
... HA and neuraminidase of relevant epidemic strains (Maassab et al., 1985). Such viruses are under trial as live attenuated influenza vaccines (Wright et al., 1982; Murphy & Clements, 1989). Because potential reference and vaccine strains of virus may also be initially isolated in mammalian cells of d ...
Runions et al - Oxford Academic
... Measurement of the rate at which activated GFP moves away from the activation spot shows that this motion is much faster than would be expected if membrane components moved simply by diffusion. Treatment with latrunculin to depolymerize the actin cytoskeleton stops ER remodelling and reduces the rat ...
... Measurement of the rate at which activated GFP moves away from the activation spot shows that this motion is much faster than would be expected if membrane components moved simply by diffusion. Treatment with latrunculin to depolymerize the actin cytoskeleton stops ER remodelling and reduces the rat ...
Vesicular stomatitis virus infects resident cells of the central nervous
... (Lundh et al., 1987). Primary murine glial cells were exposed to recombinant SeV containing the GFP gene (SeV-GFP, Fig. 1D) (Wiegand et al., 2007) at an MOIVERO of 1 (calculated based on the SeV-GFP titration on Vero cells) and viral infection and replication was again assessed by fluorescence micros ...
... (Lundh et al., 1987). Primary murine glial cells were exposed to recombinant SeV containing the GFP gene (SeV-GFP, Fig. 1D) (Wiegand et al., 2007) at an MOIVERO of 1 (calculated based on the SeV-GFP titration on Vero cells) and viral infection and replication was again assessed by fluorescence micros ...
4 | the tissue level of organization
... link cells to the extracellular matrix, for example, the basal lamina. While similar in appearance to desmosomes, they include the adhesion proteins called integrins rather than cadherins. Adherens junctions use either cadherins or integrins depending on whether they are linking to other cells or ma ...
... link cells to the extracellular matrix, for example, the basal lamina. While similar in appearance to desmosomes, they include the adhesion proteins called integrins rather than cadherins. Adherens junctions use either cadherins or integrins depending on whether they are linking to other cells or ma ...
Natural Products for the Treatment of Trachoma and Chlamydia
... group of isoquinoline alkaloids and has a long history of use in China [30]. It is found in a variety of plants [30] and it has been indicated that tinctures, powdered dried root, or fluid and solid extracts of Berberis aquifolium, Berberis vulgaris, and Hydrastis canadensis can be used orally for t ...
... group of isoquinoline alkaloids and has a long history of use in China [30]. It is found in a variety of plants [30] and it has been indicated that tinctures, powdered dried root, or fluid and solid extracts of Berberis aquifolium, Berberis vulgaris, and Hydrastis canadensis can be used orally for t ...
Sulfated polysaccharides from marine sponges
... of cellular recognition and adhesion in animals was achieved with cells from marine sponge in the beginning of the last century (Wilson 1907). This model was based on the observation that dissociated cells from marine sponge go through an aggregation process, in a species-specific way, forming small ...
... of cellular recognition and adhesion in animals was achieved with cells from marine sponge in the beginning of the last century (Wilson 1907). This model was based on the observation that dissociated cells from marine sponge go through an aggregation process, in a species-specific way, forming small ...
Abstract Panax ginseng Meyer, belonging to the genus Panax of the
... carbohydrates [45]. Animal lectins are neither immune origin nor catalyst, in contrast to antibodies or enzymes, and are able to detect or bind complex carbohydrate structures specifically through the carbohydrate-recognition domain (CRD) [46] and [47]. Each animal lectin possess its own CRD which h ...
... carbohydrates [45]. Animal lectins are neither immune origin nor catalyst, in contrast to antibodies or enzymes, and are able to detect or bind complex carbohydrate structures specifically through the carbohydrate-recognition domain (CRD) [46] and [47]. Each animal lectin possess its own CRD which h ...
Molecular Microbioiogy
... mutants and that other components that are not produced by these mutants do not seem to be directly involved in attachment. Since we were able to isolate rhicadhesin from the cell surface, lack of translocation to the cell surface is not responsible for the attachment deficiency of cfivB mutants. Th ...
... mutants and that other components that are not produced by these mutants do not seem to be directly involved in attachment. Since we were able to isolate rhicadhesin from the cell surface, lack of translocation to the cell surface is not responsible for the attachment deficiency of cfivB mutants. Th ...
The Salmonella Effector Protein SopA Modulates Innate Immune
... infection, cells were infected with S. Typhimurium prior to the analysis. We identified the melanoma differentiation-associated protein 5 (MDA5) as a TRIM65 specific interactor. This observation is intriguing since MDA5 is a member of the RNA sensor retinoic acid-inducible gene-I (RIG-I)-like recept ...
... infection, cells were infected with S. Typhimurium prior to the analysis. We identified the melanoma differentiation-associated protein 5 (MDA5) as a TRIM65 specific interactor. This observation is intriguing since MDA5 is a member of the RNA sensor retinoic acid-inducible gene-I (RIG-I)-like recept ...
Phalloidin
... Please visit www.biotium.com to view our full selection of CF™ dye and R-PE conjugates, including labeled primary and secondary antibodies, streptavidin, Annexin V, a-bungarotoxin, and Mix-n-Stain antibody labeling kits. Biotium also offers a variety of apoptosis and cell viability assays for flow c ...
... Please visit www.biotium.com to view our full selection of CF™ dye and R-PE conjugates, including labeled primary and secondary antibodies, streptavidin, Annexin V, a-bungarotoxin, and Mix-n-Stain antibody labeling kits. Biotium also offers a variety of apoptosis and cell viability assays for flow c ...
Studies on polar cell wall growth and antibiotic susceptibility of
... Understanding cellular processes in bacteria is the fundamental prerequisite for bacterial applications in biotechnology or medicine. The engineering of high efficient production strains as well as the treatment of bacterial pathogens have evolved to extremely important tasks in our society. Being f ...
... Understanding cellular processes in bacteria is the fundamental prerequisite for bacterial applications in biotechnology or medicine. The engineering of high efficient production strains as well as the treatment of bacterial pathogens have evolved to extremely important tasks in our society. Being f ...
IL-5 Receptor-mediated Tyrosine Phosphorylation of SH2
... The shc gene codes for three protein products of "~46, 52, and 66 kD (21) containing a single COOH-terminal SH2 domain. The tyrosine-phosphorylated Shc binds to Grb2 which activates Sos protein, a Ras nucleotide-exchange protein (22). Ras has been shown to work downstream of tyrosine kinases in the ...
... The shc gene codes for three protein products of "~46, 52, and 66 kD (21) containing a single COOH-terminal SH2 domain. The tyrosine-phosphorylated Shc binds to Grb2 which activates Sos protein, a Ras nucleotide-exchange protein (22). Ras has been shown to work downstream of tyrosine kinases in the ...
Cytotoxicity of 1,2-epoxynaphthalene Is Correlated with
... monooxygenases to 1,2-epoxynaphthalene. However, the subsequent interactions of the epoxide with macromolecules in the cells, and the significance of these interactions to cellular injury, are not well characterized. Additionally, CYP1A1, which can metabolize naphthalene to 1,2-epoxynaphthalene, may ...
... monooxygenases to 1,2-epoxynaphthalene. However, the subsequent interactions of the epoxide with macromolecules in the cells, and the significance of these interactions to cellular injury, are not well characterized. Additionally, CYP1A1, which can metabolize naphthalene to 1,2-epoxynaphthalene, may ...
Chapter 3 PowerPoint
... The Cytoplasm Membranous Organelles • Mitochondria • 95% of cellular ATP supply • Double membrane structure • Outer membrane very permeable • Inner membrane very impermeable Folded into cristae Filled with matrix Studded with ETS complexes ...
... The Cytoplasm Membranous Organelles • Mitochondria • 95% of cellular ATP supply • Double membrane structure • Outer membrane very permeable • Inner membrane very impermeable Folded into cristae Filled with matrix Studded with ETS complexes ...
TESIS DOCTORAL
... bioactive implant that could maintain the cells in the implanted site, afford the mechanical heart behavior and, at the same time, provide to the implanted cells a proper microenvironment. Therefore, we have prepared a composite obtained from the combination of RAD16-I included within microporous of ...
... bioactive implant that could maintain the cells in the implanted site, afford the mechanical heart behavior and, at the same time, provide to the implanted cells a proper microenvironment. Therefore, we have prepared a composite obtained from the combination of RAD16-I included within microporous of ...
Inter-specific scaling of phytoplankton production and cell size in the
... The high values of the size scaling exponent for production rate confirm recent measurements which indicated that the size scaling of phytoplankton photosynthesis in the ocean is approximately isometrical (Marañón et al., 2007). A likely explanation for this departure from the 34-power rule lies i ...
... The high values of the size scaling exponent for production rate confirm recent measurements which indicated that the size scaling of phytoplankton photosynthesis in the ocean is approximately isometrical (Marañón et al., 2007). A likely explanation for this departure from the 34-power rule lies i ...
Antisickling Activity and Membrane Stabilizing Effect of
... NaCl by increasing the volume of the RBCs, reverting the sickling to produce biconcave cells, and thereby, maintaining membrane integrity. The sickling modifies the membrane flexibility, which would make it more fragile and would increase the precocious risk of hemolysis. But it is as possible as th ...
... NaCl by increasing the volume of the RBCs, reverting the sickling to produce biconcave cells, and thereby, maintaining membrane integrity. The sickling modifies the membrane flexibility, which would make it more fragile and would increase the precocious risk of hemolysis. But it is as possible as th ...
NuMA assembles into an extensive filamentous structure when
... nucleus has been mapped to a short segment in its carboxylterminal globular tail domain (Tang et al., 1994). To test the fate of NuMA in the cell cytoplasm we have used site-directed mutagenesis to delete the nuclear localization sequence from the NuMA protein and then transiently expressed this mut ...
... nucleus has been mapped to a short segment in its carboxylterminal globular tail domain (Tang et al., 1994). To test the fate of NuMA in the cell cytoplasm we have used site-directed mutagenesis to delete the nuclear localization sequence from the NuMA protein and then transiently expressed this mut ...
immunoglobulins antigen-antibody reactions and selected tests
... chains into a number of distinct globular domains. The area where the top of the "Y" joins the bottom is called the hinge. This area is flexible to enable the antibody to bind to pairs of epitopes various distances apart on an antigen. The two tips of the "Y" monomer are referred to as the Fab por ...
... chains into a number of distinct globular domains. The area where the top of the "Y" joins the bottom is called the hinge. This area is flexible to enable the antibody to bind to pairs of epitopes various distances apart on an antigen. The two tips of the "Y" monomer are referred to as the Fab por ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.