Sampathkumar-2011-Live Cell Imaging Re - Max
... via pharmacological agents such as latrunculinB (latB) and cytochalasinB (Collings, 2008). Here, we tested whether a more stable actin configuration had an impact on the organization of MTs using the drug jasplakinolide (Bubb et al., 1994). Because jasplakinolide has not been extensively employed in ...
... via pharmacological agents such as latrunculinB (latB) and cytochalasinB (Collings, 2008). Here, we tested whether a more stable actin configuration had an impact on the organization of MTs using the drug jasplakinolide (Bubb et al., 1994). Because jasplakinolide has not been extensively employed in ...
Mixed Lineage Kinase Domain-like Protein Mediates Necrosis
... The importance of necrotic cell death has been illustrated by several recent findings. Breeding onto a RIP3 knockout background rescues the developmental defects of caspase-8 knockout mice. This indicates that the cardiac, vascular, and hematopoietic defects that occur during the development of casp ...
... The importance of necrotic cell death has been illustrated by several recent findings. Breeding onto a RIP3 knockout background rescues the developmental defects of caspase-8 knockout mice. This indicates that the cardiac, vascular, and hematopoietic defects that occur during the development of casp ...
Drac1 and Crumbs in amnioserosa morphogenesis
... towards each other. The contraction of the amnioserosa following induction of Drac1V12 was caused by a striking change in cell shape. Cell shape change in the wild-type amnioserosa is accompanied by the accumulation of F-actin, myosin and phosphotyrosine. It is not unexpected, therefore, that the le ...
... towards each other. The contraction of the amnioserosa following induction of Drac1V12 was caused by a striking change in cell shape. Cell shape change in the wild-type amnioserosa is accompanied by the accumulation of F-actin, myosin and phosphotyrosine. It is not unexpected, therefore, that the le ...
immunodetection of arabinogalactan proteins in different types of
... apparatus (Fig. 11, 12). Both studied AGP epitopes were detected inside the micropylar canal as well, but were not present in the somatic cells surrounding the canal. Other parts of the mature ovules were devoid of AGP epitopes, except for the funiculus vessels. ...
... apparatus (Fig. 11, 12). Both studied AGP epitopes were detected inside the micropylar canal as well, but were not present in the somatic cells surrounding the canal. Other parts of the mature ovules were devoid of AGP epitopes, except for the funiculus vessels. ...
Golgi Regeneration after Brefeldin A Treatment in
... vesiculation. Beginning as clusters of vesicles that are COPI positive, mini-Golgi stacks first become recognizable 60 min after BFA washout. They continue to increase in terms of numbers and length of cisternae for a further 90 min before overshooting the size of control Golgi stacks. As a result, ...
... vesiculation. Beginning as clusters of vesicles that are COPI positive, mini-Golgi stacks first become recognizable 60 min after BFA washout. They continue to increase in terms of numbers and length of cisternae for a further 90 min before overshooting the size of control Golgi stacks. As a result, ...
Cell size trade-offs govern light exploitation strategies in
... where M is the metabolic rate, a is the size-normalized metabolic rate, which varies among taxonomic groups (Irwin et al., 2006; Raven et al., 2006), E is the activation energy for metabolism, k is the Boltzmann’s constant (8.62 eV K-1), T is temperature and b is the size scaling exponent, measured ...
... where M is the metabolic rate, a is the size-normalized metabolic rate, which varies among taxonomic groups (Irwin et al., 2006; Raven et al., 2006), E is the activation energy for metabolism, k is the Boltzmann’s constant (8.62 eV K-1), T is temperature and b is the size scaling exponent, measured ...
- The University of Liverpool Repository
... determined by the trypan blue exclusion method. After dilution to a concentration of 2 x ...
... determined by the trypan blue exclusion method. After dilution to a concentration of 2 x ...
Eph/ephrins and N-cadherin coordinate to control the
... of sympathetic ganglia Given the spatiotemporal correlation between these gene expression patterns and neural crest cell movements in the interganglionic regions, we tested the hypothesis that ephrinB1 and EphB2 play a role in neural crest cell sorting. To block signaling in ovo, we utilized ephrinB ...
... of sympathetic ganglia Given the spatiotemporal correlation between these gene expression patterns and neural crest cell movements in the interganglionic regions, we tested the hypothesis that ephrinB1 and EphB2 play a role in neural crest cell sorting. To block signaling in ovo, we utilized ephrinB ...
Spherical particles of halophilic archaea correlate with exposure to
... on the possibility of microbial contamination during isolation and processing of samples, as well as uncertainty about their real age (McGenity et al., 2000; Park et al., 2009; Schubert et al., 2010). Retrieval of DNA from ancient sediments (Radax et al., 2001; Fish et al., 2002), repeated isolation ...
... on the possibility of microbial contamination during isolation and processing of samples, as well as uncertainty about their real age (McGenity et al., 2000; Park et al., 2009; Schubert et al., 2010). Retrieval of DNA from ancient sediments (Radax et al., 2001; Fish et al., 2002), repeated isolation ...
The Balance between Cell Division and Endoreplication Depends
... molecular alterations are accompanied by the development of more but smaller cells in leaves and cotyledons. Furthermore, partial loss of E2FC is sufficient to highly increase the amount of cyclin B1;1 expression in dividing and differentiated tissues, whereas overexpression of E2FC reduces the numb ...
... molecular alterations are accompanied by the development of more but smaller cells in leaves and cotyledons. Furthermore, partial loss of E2FC is sufficient to highly increase the amount of cyclin B1;1 expression in dividing and differentiated tissues, whereas overexpression of E2FC reduces the numb ...
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... Drug is selectively toxic when the cell is in a specific phase of growth—schedule dependent Effective against malignancies that proliferate rapidly Cell cycle–nonspecific Active throughout the cell cycle More effective against slowly proliferating neoplastic tissue Mosby items and derived ...
... Drug is selectively toxic when the cell is in a specific phase of growth—schedule dependent Effective against malignancies that proliferate rapidly Cell cycle–nonspecific Active throughout the cell cycle More effective against slowly proliferating neoplastic tissue Mosby items and derived ...
Full version (PDF file)
... 2009, Mukherjee 2013). They can prolong the circulation time of drugs in the blood stream and greatly decrease drug clearance by increasing molecular weight. Most frequently, polymer nanocarriers are designed to liberate the drug via controlled release, which is based on pHsensitivity, reduction or ...
... 2009, Mukherjee 2013). They can prolong the circulation time of drugs in the blood stream and greatly decrease drug clearance by increasing molecular weight. Most frequently, polymer nanocarriers are designed to liberate the drug via controlled release, which is based on pHsensitivity, reduction or ...
Role of Ime4 Protein in PHO Regulon of S.cerevisiae.
... second most common form of such post transcriptional modification in eukaryotic RNA is adenosine methylation by methyltransferase proteins to produce N6-methyladenosine (m6A) (Fig ...
... second most common form of such post transcriptional modification in eukaryotic RNA is adenosine methylation by methyltransferase proteins to produce N6-methyladenosine (m6A) (Fig ...
Author`s personal copy
... cell cycle lengthening. For example, in Xenopus the nucleocytoplasmic (N/C) ratio increases with each round of cell division causing the titration of an as yet unidentified factor leading to a lengthening of S phase at the midblastula transition or MBT (Newport and Kirschner, 1982a; Vastag et al., 20 ...
... cell cycle lengthening. For example, in Xenopus the nucleocytoplasmic (N/C) ratio increases with each round of cell division causing the titration of an as yet unidentified factor leading to a lengthening of S phase at the midblastula transition or MBT (Newport and Kirschner, 1982a; Vastag et al., 20 ...
The biochemistry of differentiation of mouse
... female. After 10 days incubation, the haemorrhagic growths were removed and the cells teased apart. As in the case with mature uterine trophoblast, many large nuclei fall to the bottom of the dissecting dish during this procedure. These nuclei are highly poplyploid (Fig. 6), the largest being equiva ...
... female. After 10 days incubation, the haemorrhagic growths were removed and the cells teased apart. As in the case with mature uterine trophoblast, many large nuclei fall to the bottom of the dissecting dish during this procedure. These nuclei are highly poplyploid (Fig. 6), the largest being equiva ...
Analysis of the Juxtamembrane Dileucine Motif in the Insulin Receptor
... of HeLa cells were placed in 9 ml fresh growth medium (see above). For each 10-cm plate, 10 –15 mg DNA were mixed with 64 ml 2 m CaCl2 and brought to a final volume of 0.5 ml with water. The DNA/calcium phosphate mixture was then slowly added to 0.5 ml 2 3 HEPES-buffered saline (280 mm NaCl, 1.5 mm ...
... of HeLa cells were placed in 9 ml fresh growth medium (see above). For each 10-cm plate, 10 –15 mg DNA were mixed with 64 ml 2 m CaCl2 and brought to a final volume of 0.5 ml with water. The DNA/calcium phosphate mixture was then slowly added to 0.5 ml 2 3 HEPES-buffered saline (280 mm NaCl, 1.5 mm ...
Divergent mechanisms underlie Smad4
... Background: Functional inactivation of the tumor suppressor Smad4 in colorectal and pancreatic carcinogenesis occurs coincident with the transition to invasive growth. Breaking the basement membrane (BM) barrier, a prerequisite for invasive growth, can be due to tumor induced proteolytic tissue remo ...
... Background: Functional inactivation of the tumor suppressor Smad4 in colorectal and pancreatic carcinogenesis occurs coincident with the transition to invasive growth. Breaking the basement membrane (BM) barrier, a prerequisite for invasive growth, can be due to tumor induced proteolytic tissue remo ...
Università degli Studi di Milano
... Recent data estimate that cannabis use starts in the period from 12 to 18 years of age, though even earlier use (9–10 years) is now anecdotally reported. Among the many consequences related to the cannabis use, it is increasingly emerging as the Δ9-THC, as well as endogenous cannabinoids, are able t ...
... Recent data estimate that cannabis use starts in the period from 12 to 18 years of age, though even earlier use (9–10 years) is now anecdotally reported. Among the many consequences related to the cannabis use, it is increasingly emerging as the Δ9-THC, as well as endogenous cannabinoids, are able t ...
Histone deacetylase inhibitors and the promise of epigenetic (and
... zinc-dependent catalytic domain with a high degree of homology. Less conserved are accessory domains, which seem to fulfill regulatory functions. In mammals, class 1 HDACs (HDAC1–3 and HDAC8) are related to the yeast RPD3 HDAC; class 2 HDACs (HDAC4–7, HDAC9 and HDAC10) are related to the yeast HDA1 ...
... zinc-dependent catalytic domain with a high degree of homology. Less conserved are accessory domains, which seem to fulfill regulatory functions. In mammals, class 1 HDACs (HDAC1–3 and HDAC8) are related to the yeast RPD3 HDAC; class 2 HDACs (HDAC4–7, HDAC9 and HDAC10) are related to the yeast HDA1 ...
Maurizio Gentile Role of mTOR in the Activation of Prof. Andrea Cerutti
... stimulating T cell-dependent (TD) antibody responses in follicular B cells. In adults, vaccination with native CPSs provides protection by simulating T cell-independent (TI) antibody responses in MZ B cells. However, this protection vanishes in individuals that undergo splenectomy as a result of tr ...
... stimulating T cell-dependent (TD) antibody responses in follicular B cells. In adults, vaccination with native CPSs provides protection by simulating T cell-independent (TI) antibody responses in MZ B cells. However, this protection vanishes in individuals that undergo splenectomy as a result of tr ...
The Human Arp2/3 Complex Is Composed of Evolutionarily
... were grown in DME-H16 medium with 10% FBS and 10% heat-inactivated chicken serum, penicillin, and streptomycin. Immunofluorescence experiments were carried out within 20 h of plating, which was shown by Cramer et al. (1997) to be optimal for observing rapidly locomoting cells. For immunofluorescence ...
... were grown in DME-H16 medium with 10% FBS and 10% heat-inactivated chicken serum, penicillin, and streptomycin. Immunofluorescence experiments were carried out within 20 h of plating, which was shown by Cramer et al. (1997) to be optimal for observing rapidly locomoting cells. For immunofluorescence ...
Inflammation Protein L13a in Macrophages Resolves An
... genotyping are shown (left panel ). rES cells were genotyped by PCR using Lox1/SDL2 primers and identified on the basis of the appearance of a doublet band of 325/263 bp (right panel ). (B) Confirmation of the presence of conditional null allele in rES cells. rES cells were infected by Ad-CMV-Cre (V ...
... genotyping are shown (left panel ). rES cells were genotyped by PCR using Lox1/SDL2 primers and identified on the basis of the appearance of a doublet band of 325/263 bp (right panel ). (B) Confirmation of the presence of conditional null allele in rES cells. rES cells were infected by Ad-CMV-Cre (V ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.