Methods_Mol._Biol._591_185-199
... contribute to the fluorescence signal. Confining the signal to such a subdiffraction-sized spot results in higher resolution imaging when the spot is scanned through the specimen (1, 6–8). Experiments using single molecules as test objects already demonstrated a spot size as small as 16 nm in the fo ...
... contribute to the fluorescence signal. Confining the signal to such a subdiffraction-sized spot results in higher resolution imaging when the spot is scanned through the specimen (1, 6–8). Experiments using single molecules as test objects already demonstrated a spot size as small as 16 nm in the fo ...
O-GlcNAc transferase inhibitors: current tools and
... Other compounds that have been constructed to target OGT directly can be classified into two categories: small molecule and bisubstrate inhibitors. The first group mimics and competes with the donor substrate and includes UDP, alloxan, compound 4, BZX and OSMI-1. However, most of these are likely to ...
... Other compounds that have been constructed to target OGT directly can be classified into two categories: small molecule and bisubstrate inhibitors. The first group mimics and competes with the donor substrate and includes UDP, alloxan, compound 4, BZX and OSMI-1. However, most of these are likely to ...
Autophagy and cell death in model organisms - IMBB
... summoned to serve additional cellular functions. Numerous recent studies indicate that apart from its pro-survival role under nutrient limitation, autophagy also participates in cell death. However, the precise role of this catabolic process in dying cells is not fully understood. Although in certai ...
... summoned to serve additional cellular functions. Numerous recent studies indicate that apart from its pro-survival role under nutrient limitation, autophagy also participates in cell death. However, the precise role of this catabolic process in dying cells is not fully understood. Although in certai ...
Effects of phytanic acid on the vitamin E status, lipid composition and
... function of vitamin E because of the structural similarity between these two molecules [7] (see Figure 1). In support of this hypothesis, a severe deficiency of vitamin E results in a spectrum of features similar to those observed in ARD, including peripheral and central neuropathy and retinal degen ...
... function of vitamin E because of the structural similarity between these two molecules [7] (see Figure 1). In support of this hypothesis, a severe deficiency of vitamin E results in a spectrum of features similar to those observed in ARD, including peripheral and central neuropathy and retinal degen ...
Human HL-60 Myeloid Leukemia Cells Transport
... HL-60 cells.Transport of DHA was completed by D- but notLhexoses, and was sensitive to D-hexose-dependent counter transport acceleration. These data support the concept that HL-60 myeloid leukemic cells transport DHA through the facilitative hexose transporters (glucose transporters) and accumulate ...
... HL-60 cells.Transport of DHA was completed by D- but notLhexoses, and was sensitive to D-hexose-dependent counter transport acceleration. These data support the concept that HL-60 myeloid leukemic cells transport DHA through the facilitative hexose transporters (glucose transporters) and accumulate ...
Calcium-induced calcium release supports recruitment of synaptic
... electrical properties of hair cells (Figure 3). The responses of hair cells recorded in current- ...
... electrical properties of hair cells (Figure 3). The responses of hair cells recorded in current- ...
Cell death by autophagy: facts and apparent artefacts
... cellular stress, as caused by starvation and increased metabolism (as in cancer cells), it is not surprising that cells undergoing death after stress also show features of autophagy. For example, cultured cells deprived of survival factors (such as serum) will undergo apoptosis, but as they go throu ...
... cellular stress, as caused by starvation and increased metabolism (as in cancer cells), it is not surprising that cells undergoing death after stress also show features of autophagy. For example, cultured cells deprived of survival factors (such as serum) will undergo apoptosis, but as they go throu ...
Out of the Mouths of Plants: The Molecular Basis of the Evolution
... The evolution of stomata (Greek for mouths; singular, stoma) was a crucial adaptation occurring some 400 million years ago that made it possible for plants to thrive on land, which they began to colonize some 450 million years ago. To survive in the dry atmosphere, plants must protect against desicc ...
... The evolution of stomata (Greek for mouths; singular, stoma) was a crucial adaptation occurring some 400 million years ago that made it possible for plants to thrive on land, which they began to colonize some 450 million years ago. To survive in the dry atmosphere, plants must protect against desicc ...
Molecular Machinery Regulating Exocytosis
... Rabs have important interactive functions at different stages of exocytosis. Protein sorting in recycling endosomes depends upon the function of the small GTPase Rab4, a close homologue of Rab11 (Li, et al., 2008; Ward, et al., 2005). Rab3 also has a role in anterograde traffic between the trans-Gol ...
... Rabs have important interactive functions at different stages of exocytosis. Protein sorting in recycling endosomes depends upon the function of the small GTPase Rab4, a close homologue of Rab11 (Li, et al., 2008; Ward, et al., 2005). Rab3 also has a role in anterograde traffic between the trans-Gol ...
In vitro skin models to study epithelial regeneration from the hair
... freshly excised wound bed, completion of dermal regeneration takes up to 4 weeks, after which, the silicone layer is replaced by a thin split-skin graft to achieve permanent epidermal wound closure [13]. Cultured autologous keratinocytes [14], and cultured skin substitutes [15] have been used as sec ...
... freshly excised wound bed, completion of dermal regeneration takes up to 4 weeks, after which, the silicone layer is replaced by a thin split-skin graft to achieve permanent epidermal wound closure [13]. Cultured autologous keratinocytes [14], and cultured skin substitutes [15] have been used as sec ...
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... The sarcoplasmic reticulum (SR), although not arranged to form the typical transverse tubule system (the triad) with the extracellular membrane, is present at several locations and, similar to vertebrate striated muscle, might serve as calcium storage (Kier, 1985). At the fiber border the SR forms t ...
... The sarcoplasmic reticulum (SR), although not arranged to form the typical transverse tubule system (the triad) with the extracellular membrane, is present at several locations and, similar to vertebrate striated muscle, might serve as calcium storage (Kier, 1985). At the fiber border the SR forms t ...
Bacterial metapopulations in nanofabricated
... landscape). Fig. 1B shows our solution to this problem: microhabitat patches (MHPs) that allow distinct local populations to fill a given habitat patch of quality parameters determined by nanoslits linking each MHP to external feeding channels. Individual MHPs have coupling corridors. Thus, a specie ...
... landscape). Fig. 1B shows our solution to this problem: microhabitat patches (MHPs) that allow distinct local populations to fill a given habitat patch of quality parameters determined by nanoslits linking each MHP to external feeding channels. Individual MHPs have coupling corridors. Thus, a specie ...
Host cell-derived lactate functions as an effector molecule in
... The aim of this study was to investigate the process of N. meningitidis microcolony dispersal. To evaluate the necessity of direct interaction with host cells, we monitored the timing of the dispersal upon infection of pharyngeal epithelial FaDu cells at different confluences using livecell time-lap ...
... The aim of this study was to investigate the process of N. meningitidis microcolony dispersal. To evaluate the necessity of direct interaction with host cells, we monitored the timing of the dispersal upon infection of pharyngeal epithelial FaDu cells at different confluences using livecell time-lap ...
... there is a need for rapid and reliable tests that discriminate C. gattii from Cryptococcus neoformans (serotypes A, D, and AD). Seventy-two C. neoformans strains, sixty-seven C. gattii strains, and five Candida albicans strains were analyzed for their ability to grow and produce pigment on minimal D ...
Role of CD26-adenosine deaminase interaction in T cell
... expression is induced by stimuli that favour the development of Th1 responses (8). The correlation between the CD26 expression and Th1-like immune responses has been suggested to be due to an IL-12-dependent up-regulation(9). In this regard, it is hypothesized that CD26 expression on T cell is ...
... expression is induced by stimuli that favour the development of Th1 responses (8). The correlation between the CD26 expression and Th1-like immune responses has been suggested to be due to an IL-12-dependent up-regulation(9). In this regard, it is hypothesized that CD26 expression on T cell is ...
Guidelines on evaluation of monoclonal antibodies as similar
... clinical efficacy, other effects may also play a role. In some cases multiple functions of the mAb may be involved in an additive or synergistic manner to produce an overall combined clinical effect, and this may be hard to dissect experimentally to allow a clear understanding of how the mAb mediate ...
... clinical efficacy, other effects may also play a role. In some cases multiple functions of the mAb may be involved in an additive or synergistic manner to produce an overall combined clinical effect, and this may be hard to dissect experimentally to allow a clear understanding of how the mAb mediate ...
Animal Biology 56(4)
... been used as a criterion for homology, but this becomes problematic when skull elements such as the parietal bone are now shown, by modern fate-mapping studies, to have different developmental origins in different groups of tetrapods. This indicates a flexibility of developmental programmes and regu ...
... been used as a criterion for homology, but this becomes problematic when skull elements such as the parietal bone are now shown, by modern fate-mapping studies, to have different developmental origins in different groups of tetrapods. This indicates a flexibility of developmental programmes and regu ...
The Molecular Basis of the Evolution and Diversity of Stomatal
... The evolution of stomata (Greek for mouths; singular, stoma) was a crucial adaptation occurring some 400 million years ago that made it possible for plants to thrive on land, which they began to colonize some 450 million years ago. To survive in the dry atmosphere, plants must protect against desicc ...
... The evolution of stomata (Greek for mouths; singular, stoma) was a crucial adaptation occurring some 400 million years ago that made it possible for plants to thrive on land, which they began to colonize some 450 million years ago. To survive in the dry atmosphere, plants must protect against desicc ...
Discovery and Characterization of a Novel Microtubule
... Cell walls are a distinct feature of plants and their chemical constituents, cellulose, hemicelluloses and lignin, are economically valuable. Plant fibres rich in cellulose, which mainly resides in their cell wall, are traditionally used in making paper and textiles. The changing global economic sit ...
... Cell walls are a distinct feature of plants and their chemical constituents, cellulose, hemicelluloses and lignin, are economically valuable. Plant fibres rich in cellulose, which mainly resides in their cell wall, are traditionally used in making paper and textiles. The changing global economic sit ...
Local contrast in natural images: normalisation
... on the second-order statistics, specifically on the correlation between pairs of luminance values at different points in an image (Burton and Moorhead 1987; Field 1987; Tolhurst et al 1992). Neighbouring points in natural scenes are highly correlated, with correlation falling exponentially as the di ...
... on the second-order statistics, specifically on the correlation between pairs of luminance values at different points in an image (Burton and Moorhead 1987; Field 1987; Tolhurst et al 1992). Neighbouring points in natural scenes are highly correlated, with correlation falling exponentially as the di ...
Rat Retinal Pigment Epithelial Cells Show Specificity of
... swelling of ROS (29), while low temperature (4°C) and low pH (6.0) were used to minimize actin microfilament destruction by osmium tetroxide (39). Fixed cell colonies were stored overnight in 0.2 M cacodylate buffer conraining 7% sucrose. Coverslips were rinsed 2 x 5 min with water, dehydrated rapid ...
... swelling of ROS (29), while low temperature (4°C) and low pH (6.0) were used to minimize actin microfilament destruction by osmium tetroxide (39). Fixed cell colonies were stored overnight in 0.2 M cacodylate buffer conraining 7% sucrose. Coverslips were rinsed 2 x 5 min with water, dehydrated rapid ...
Genetic and molecular analysis of Sanfilippo C
... Several therapies have been tested for different LSDs including selective treatments as enzyme replacement therapy (ERT), substrate reduction therapy (SRT), use of chemical chaperones and cell therapy using bone marrow transplantation (BMT), mesenchymal stem cells (MSC) or neural st ...
... Several therapies have been tested for different LSDs including selective treatments as enzyme replacement therapy (ERT), substrate reduction therapy (SRT), use of chemical chaperones and cell therapy using bone marrow transplantation (BMT), mesenchymal stem cells (MSC) or neural st ...
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... The cDNA corresponding to amino acids 994-1094 of Lgl2 was cloned into the pQE-30 UA vector in M15(pREP4) cells. The expressed His-tagged protein was isolated and affinity purified under native conditions (The Qiaexpressionist, Qiagen) and used for immunisation. A peptide polyclonal antibody against ...
... The cDNA corresponding to amino acids 994-1094 of Lgl2 was cloned into the pQE-30 UA vector in M15(pREP4) cells. The expressed His-tagged protein was isolated and affinity purified under native conditions (The Qiaexpressionist, Qiagen) and used for immunisation. A peptide polyclonal antibody against ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.