Lack of correlation between trehalose accumulation, cell viability
... disaccharide synthesis could be attributed t o a twofold higher trehalose-6phosphate synthase activity, together with a fourfold higher content of intracellular UDP-Glc. In addition, this mutant showed 1.5-fold higher levels of ATP compared to the wild-type. The various stress treatments studied sho ...
... disaccharide synthesis could be attributed t o a twofold higher trehalose-6phosphate synthase activity, together with a fourfold higher content of intracellular UDP-Glc. In addition, this mutant showed 1.5-fold higher levels of ATP compared to the wild-type. The various stress treatments studied sho ...
A novel genetic strategy reveals unexpected roles of the Swi
... (Fig. 2 A, column 1). The immature thymocytes are then resolved into two major subsets, namely DN3 (CD25+CD44⫺) and post-DN3 (CD25⫺CD44⫺; Fig. 2 A, column 2) before each population is analyzed for CD4/CD8 expression (columns 3–5). In WT mice, the DN3 cells are, by definition, CD4⫺CD8⫺ (Fig. 2 A, row ...
... (Fig. 2 A, column 1). The immature thymocytes are then resolved into two major subsets, namely DN3 (CD25+CD44⫺) and post-DN3 (CD25⫺CD44⫺; Fig. 2 A, column 2) before each population is analyzed for CD4/CD8 expression (columns 3–5). In WT mice, the DN3 cells are, by definition, CD4⫺CD8⫺ (Fig. 2 A, row ...
Update on amniotic membrane transplantation
... AM has been used as an ideal substrate to cultivate epithelial progenitor cells of the conjunctival epithelium [42–46] , corneal epithelium [47–50] , limbus [45,51–59] , oral mucosa [49,60–62] and corneal endothelium [63,64] . The techniques used in these studies include using either explants or sus ...
... AM has been used as an ideal substrate to cultivate epithelial progenitor cells of the conjunctival epithelium [42–46] , corneal epithelium [47–50] , limbus [45,51–59] , oral mucosa [49,60–62] and corneal endothelium [63,64] . The techniques used in these studies include using either explants or sus ...
PDF - The Gould Lab
... eyeblink conditioning (200 trials/d for 4 d) using a trace (n ⫽ 8), delay (n ⫽ 7), or very long delay (n ⫽ 11) procedure (Fig. 1). An additional group of naive rats (n ⫽ 10) was injected with BrdU but did not receive stimulus exposure. All rats were perfused 24 h after the last block of training tri ...
... eyeblink conditioning (200 trials/d for 4 d) using a trace (n ⫽ 8), delay (n ⫽ 7), or very long delay (n ⫽ 11) procedure (Fig. 1). An additional group of naive rats (n ⫽ 10) was injected with BrdU but did not receive stimulus exposure. All rats were perfused 24 h after the last block of training tri ...
histology cytology embryology
... A variant of electron microscopy, scanning electron microscopy, and permits pseudo-three-dimensional remembered that the observed product is the end result of a series of processes that considerably distort the image observable in living tissue, mainly through shrinkage. This shrinkage is produced m ...
... A variant of electron microscopy, scanning electron microscopy, and permits pseudo-three-dimensional remembered that the observed product is the end result of a series of processes that considerably distort the image observable in living tissue, mainly through shrinkage. This shrinkage is produced m ...
Staphylococcus aureus , and Streptococcus pneumoniae , coli
... chamber. Interestingly, the effects of cycloheximide varied substantially according to the stimulus. Migration to intact S. pneumoniae did not require new protein synthesis with migration after cycloheximide at 98.9% of nontreated monolayers. Migration to E. coli was substantially inhibited (69.3% o ...
... chamber. Interestingly, the effects of cycloheximide varied substantially according to the stimulus. Migration to intact S. pneumoniae did not require new protein synthesis with migration after cycloheximide at 98.9% of nontreated monolayers. Migration to E. coli was substantially inhibited (69.3% o ...
the physiology of mechanoelectrical transduction channels in hearing
... at the tops of the stereocilia. These channels are cation-permeable with a high selectivity for Ca2⫹; two channels are thought to be localized at the lower end of the tip link, each with a large single-channel conductance that increases from the low- to high-frequency end of the cochlea. Ca2⫹ influx ...
... at the tops of the stereocilia. These channels are cation-permeable with a high selectivity for Ca2⫹; two channels are thought to be localized at the lower end of the tip link, each with a large single-channel conductance that increases from the low- to high-frequency end of the cochlea. Ca2⫹ influx ...
Ca2+ signalling and control of guard-cell volume in stomatal
... plasma membrane because mature guard cells lack functional plasmodesmata [1,2]. K+ transport is dominated by two classes of K+ channels. The first of these permits K+ movement into, but not out of, the cell and thus gives rise to an inward-rectifying current (IK,in); the second facilitates K+ flux o ...
... plasma membrane because mature guard cells lack functional plasmodesmata [1,2]. K+ transport is dominated by two classes of K+ channels. The first of these permits K+ movement into, but not out of, the cell and thus gives rise to an inward-rectifying current (IK,in); the second facilitates K+ flux o ...
TEAD4 establishes the energy homeostasis essential for blastocoel
... The earliest cell differentiation event in mammalian development occurs during preimplantation development when the outer blastomeres of the embryo form a monolayer of epithelial cells [trophectoderm (TE)] that envelops the remaining blastomeres [inner cell mass (ICM)]. In mice, TE specification occ ...
... The earliest cell differentiation event in mammalian development occurs during preimplantation development when the outer blastomeres of the embryo form a monolayer of epithelial cells [trophectoderm (TE)] that envelops the remaining blastomeres [inner cell mass (ICM)]. In mice, TE specification occ ...
The tumor suppressor Scrib interacts with the zyxin
... shown that the three LIM domains of LPP have transcriptional activation capacity [11], this bait, although well expressed, did not activate the reporter genes in yeast cells (results not shown). This is similar to what has been found for zyxin's LIM domains [22], but in contrast to what has been fou ...
... shown that the three LIM domains of LPP have transcriptional activation capacity [11], this bait, although well expressed, did not activate the reporter genes in yeast cells (results not shown). This is similar to what has been found for zyxin's LIM domains [22], but in contrast to what has been fou ...
Complement-dependent immune complex-induced - AJP-Lung
... complement-depleted animals after immune complex deposition (Fig. 2C), whereas in complement-intact mice, a marked neutrophil infiltration was detected in the bronchial wall (Fig. 2, B and D). The data presented in Fig. 3 clearly demonstrate that when complement was inhibited by pretreatment with CV ...
... complement-depleted animals after immune complex deposition (Fig. 2C), whereas in complement-intact mice, a marked neutrophil infiltration was detected in the bronchial wall (Fig. 2, B and D). The data presented in Fig. 3 clearly demonstrate that when complement was inhibited by pretreatment with CV ...
Electron Tomographic Analysis of Somatic Cell Plate Formation in
... of plant cytokinesis: cytokinesis starts in late anaphase with the formation of the phragmoplast from anaphase spindle microtubules. The phragmoplast consists of two opposing arrays of actin filaments and microtubules with their (1) ends facing the plane of division. Its primary function is to trans ...
... of plant cytokinesis: cytokinesis starts in late anaphase with the formation of the phragmoplast from anaphase spindle microtubules. The phragmoplast consists of two opposing arrays of actin filaments and microtubules with their (1) ends facing the plane of division. Its primary function is to trans ...
Electron Tomographic Analysis of Somatic Cell Plate Formation in
... of plant cytokinesis: cytokinesis starts in late anaphase with the formation of the phragmoplast from anaphase spindle microtubules. The phragmoplast consists of two opposing arrays of actin filaments and microtubules with their (1) ends facing the plane of division. Its primary function is to trans ...
... of plant cytokinesis: cytokinesis starts in late anaphase with the formation of the phragmoplast from anaphase spindle microtubules. The phragmoplast consists of two opposing arrays of actin filaments and microtubules with their (1) ends facing the plane of division. Its primary function is to trans ...
Electron Tomographic Analysis of Somatic Cell
... of plant cytokinesis: cytokinesis starts in late anaphase with the formation of the phragmoplast from anaphase spindle microtubules. The phragmoplast consists of two opposing arrays of actin filaments and microtubules with their (1) ends facing the plane of division. Its primary function is to trans ...
... of plant cytokinesis: cytokinesis starts in late anaphase with the formation of the phragmoplast from anaphase spindle microtubules. The phragmoplast consists of two opposing arrays of actin filaments and microtubules with their (1) ends facing the plane of division. Its primary function is to trans ...
pitx3 defines an equivalence domain for lens and anterior pituitary
... transplanted 10 to 20 cells from the animal pole to wild-type host animal poles. Donor and host embryos were maintained as pairs in 24-well dishes in penicillin and streptomycin (1% each) in Danieau’s solution. At prim-5 stage, donors were analyzed for loss of lens phenotype that resulted from dnPKA ...
... transplanted 10 to 20 cells from the animal pole to wild-type host animal poles. Donor and host embryos were maintained as pairs in 24-well dishes in penicillin and streptomycin (1% each) in Danieau’s solution. At prim-5 stage, donors were analyzed for loss of lens phenotype that resulted from dnPKA ...
Dynamics of the Cell Cycle: Checkpoints, Sizers, and Timers
... insight into this most fundamental biological process. A number of mathematical models (Aguda, 1999; Aguda and Tang, 1999; Chen et al., 2000; Gardner et al., 1998; Goldbeter, 1991; Hatzimanikatis et al., 1999; Novak and Tyson, 1997; Obeyesekere et al., 1997; Qu et al., 2003; Sveiczer et al., 2000; T ...
... insight into this most fundamental biological process. A number of mathematical models (Aguda, 1999; Aguda and Tang, 1999; Chen et al., 2000; Gardner et al., 1998; Goldbeter, 1991; Hatzimanikatis et al., 1999; Novak and Tyson, 1997; Obeyesekere et al., 1997; Qu et al., 2003; Sveiczer et al., 2000; T ...
Leaf development - The Virtual Plant
... The shoot apical meristem has an intrinsic capacity for self regulation and the positional specification of its cells implies the existence of an elaborate and versatile communication network. Van der Schoot and Rinne2 proposed a model that pictures this network as a system of overlapping signal cir ...
... The shoot apical meristem has an intrinsic capacity for self regulation and the positional specification of its cells implies the existence of an elaborate and versatile communication network. Van der Schoot and Rinne2 proposed a model that pictures this network as a system of overlapping signal cir ...
Signalling and neural crest migration pathways - Development
... the generation of crest cells from odd rhombomeres. In contrast, lineage and time-lapse analyses in avians clearly show that r3 and r5 do generate neural crest cells, but they move anteriorly and posteriorly, joining the even rhombomere streams (Kulesa, 1998; Kulesa et al., 2000; Kulesa and Fraser, ...
... the generation of crest cells from odd rhombomeres. In contrast, lineage and time-lapse analyses in avians clearly show that r3 and r5 do generate neural crest cells, but they move anteriorly and posteriorly, joining the even rhombomere streams (Kulesa, 1998; Kulesa et al., 2000; Kulesa and Fraser, ...
Abstract book 2014 - Arbeitsgemeinschaft Dermatologische Forschung
... Dresden, Dresden, Germany; 3Department of Dermatology and Allergy, Hanover Medical School, Hanover, Germany; 4Institute of Pathology, University of Leipzig, Leipzig, Germany Mastocytosis is characterized by pathologic accumulation of mast cells in different tissues, particularly in skin and bone mar ...
... Dresden, Dresden, Germany; 3Department of Dermatology and Allergy, Hanover Medical School, Hanover, Germany; 4Institute of Pathology, University of Leipzig, Leipzig, Germany Mastocytosis is characterized by pathologic accumulation of mast cells in different tissues, particularly in skin and bone mar ...
brachyenteron function in the mesoderm
... of different taxa share high structural similarites in functionally relevant domains, such as DNA-binding or protein-protein interaction domains. These similarities allow factors to be grouped into families such as homeodomain proteins or Wnt proteins (for reviews see e.g. McGinnis and Krumlauf, 199 ...
... of different taxa share high structural similarites in functionally relevant domains, such as DNA-binding or protein-protein interaction domains. These similarities allow factors to be grouped into families such as homeodomain proteins or Wnt proteins (for reviews see e.g. McGinnis and Krumlauf, 199 ...
Salinity Effects on the Activity of Plasma Membrane H+ and Ca2+
... external NaCl aect plasma membrane Ca2 and H transporters. Some authors have reported rapid elevation of cytosolic free calcium, [Ca2 ]cyt , in response to NaCl treatment (Bittisnich et al., 1989; Lynch et al., 1989; Okazaki et al., 1996), although others attribute these data to methodological ...
... external NaCl aect plasma membrane Ca2 and H transporters. Some authors have reported rapid elevation of cytosolic free calcium, [Ca2 ]cyt , in response to NaCl treatment (Bittisnich et al., 1989; Lynch et al., 1989; Okazaki et al., 1996), although others attribute these data to methodological ...
reproduction and bacterial symbiosis in
... and ultrastructural analysis of morphological characteristics occurring in the sequence of events during sexual reproductive processes. ...
... and ultrastructural analysis of morphological characteristics occurring in the sequence of events during sexual reproductive processes. ...
The regulation and plasticity of root hair patterning
... the absorptive area, particularly in the Pi-rich topsoil layer. The coordination of shoot and root responses involves trafficking of systemic signals, such as sugar, microRNAs and hormones, through the vasculature to tune the responses in roots (Lin et al., 2014). The morphological responses of root ...
... the absorptive area, particularly in the Pi-rich topsoil layer. The coordination of shoot and root responses involves trafficking of systemic signals, such as sugar, microRNAs and hormones, through the vasculature to tune the responses in roots (Lin et al., 2014). The morphological responses of root ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.