by MICHAEL WING-YIN WU
... ABSTRACT Melanoma is the most deadly form of skin cancers, with an incidence increasing more rapidly than any other malignant cancer in the past 40 years. Metastatic melanoma is resistant to conventional treatments, such as chemotherapy and radiation therapy. Our lab has previously demonstrated tha ...
... ABSTRACT Melanoma is the most deadly form of skin cancers, with an incidence increasing more rapidly than any other malignant cancer in the past 40 years. Metastatic melanoma is resistant to conventional treatments, such as chemotherapy and radiation therapy. Our lab has previously demonstrated tha ...
Recent developments in the cell and molecular biology of root hairs
... Growing root hairs of Arabidopsis have been shown to have two ion transport processes (Lew 1991, using micropipette techniques). One of these is a tetra ethyl ammonium-sensitive potassium ion current, and experiments showed that at resting potential there is a net inward potassium current. This pota ...
... Growing root hairs of Arabidopsis have been shown to have two ion transport processes (Lew 1991, using micropipette techniques). One of these is a tetra ethyl ammonium-sensitive potassium ion current, and experiments showed that at resting potential there is a net inward potassium current. This pota ...
Plant physiology
... 1992; Thompson, 1996). In green microalgae (Chlorophyceae), TAGs are usually synthesized and accumulated in cytoplasmatic lipid droplets (CLDs; Murphy, 2012), although in some cases, such as in Chlamydomonas reinhardtii starchless mutants, they also accumulate in chloroplasts (Fan et al., 2011; Good ...
... 1992; Thompson, 1996). In green microalgae (Chlorophyceae), TAGs are usually synthesized and accumulated in cytoplasmatic lipid droplets (CLDs; Murphy, 2012), although in some cases, such as in Chlamydomonas reinhardtii starchless mutants, they also accumulate in chloroplasts (Fan et al., 2011; Good ...
Normal Thermoregulatory Responses to 3-iodothyronamine, Trace Amines and Amphetamine-like Psychostimulants in
... synaptosome preparations from different sources, and accordingly, data are normalized to the maximum level or the baseline and expressed as percentage values in each set of assays. Data for the drug effects on thermoregulation were normalized to the saline controls prior to statistical analyses. A t ...
... synaptosome preparations from different sources, and accordingly, data are normalized to the maximum level or the baseline and expressed as percentage values in each set of assays. Data for the drug effects on thermoregulation were normalized to the saline controls prior to statistical analyses. A t ...
A cellular backline: specialization of host membranes for defence
... flagellin and mediates the initiation of a suite of antibacterial defences including ROS burst, Ca2+ influx, and callose deposition. Flagellin binding occurs while FLS2 is resident in the plasma membrane, triggering accumulation of FLS2 in detergent-resistant membrane microdomains (Keinath et al., ...
... flagellin and mediates the initiation of a suite of antibacterial defences including ROS burst, Ca2+ influx, and callose deposition. Flagellin binding occurs while FLS2 is resident in the plasma membrane, triggering accumulation of FLS2 in detergent-resistant membrane microdomains (Keinath et al., ...
A Trojan Horse in Drug Development: Targeting of Thapsigargins
... Prostate cancer at the T1 or T2 stage is often successfully treated by radical prostatectomy or radiotherapy. In the Scandinavian countries, however, prostate cancer in these stages is not always treated, but the status of the patients is kept under close surveillance (watchful waiting). The rationa ...
... Prostate cancer at the T1 or T2 stage is often successfully treated by radical prostatectomy or radiotherapy. In the Scandinavian countries, however, prostate cancer in these stages is not always treated, but the status of the patients is kept under close surveillance (watchful waiting). The rationa ...
Failsafe Mechanisms Coordinate Cell Division and the Initiation of
... This view of the cell cycle is complicated when cells are grown in rich media. Bacteria can have doubling rates that are ~20-‐25 minutes, which is much faster than the time it takes for chromosom ...
... This view of the cell cycle is complicated when cells are grown in rich media. Bacteria can have doubling rates that are ~20-‐25 minutes, which is much faster than the time it takes for chromosom ...
Subcellular trafficking kinetics of GLUT4 mutated at the N
... intracellular retention. These results are also considered in terms of a consecutive intracellular pool model in which GLUT4 targeting domains alter the distribution between recycling endosomes and a slowly recycling compartment. In this case the more rapid apparent exocytosis of the mutated GLUT4s ...
... intracellular retention. These results are also considered in terms of a consecutive intracellular pool model in which GLUT4 targeting domains alter the distribution between recycling endosomes and a slowly recycling compartment. In this case the more rapid apparent exocytosis of the mutated GLUT4s ...
Avian erythrocytes have functional mitochondria, opening
... on glycolysis to fuel their own energy demanding processes [6,7]. Second, the loss of mitochondria might lessen the exposure of mammalian erythrocytes to the potentially deleterious production of mitochondrial ROS [6], with the theoretical benefit of maximizing their lifespan. The potential implicat ...
... on glycolysis to fuel their own energy demanding processes [6,7]. Second, the loss of mitochondria might lessen the exposure of mammalian erythrocytes to the potentially deleterious production of mitochondrial ROS [6], with the theoretical benefit of maximizing their lifespan. The potential implicat ...
Identification and localization of the multiple bacterial
... probe, up to two unlabelled helper probes were designed to increase the accessibility of the labelled probe to the SSU rRNA (Fuchs et al. 2000; Table 1). Hindguts were opened in PBS (137 mM NaCl, 4.3 mM Na2HPO4, 2.7 mM KCl, 1.47 mM KH2PO4; pH 7.4). The sample was fixed with 4 % (v/v) paraformaldehyd ...
... probe, up to two unlabelled helper probes were designed to increase the accessibility of the labelled probe to the SSU rRNA (Fuchs et al. 2000; Table 1). Hindguts were opened in PBS (137 mM NaCl, 4.3 mM Na2HPO4, 2.7 mM KCl, 1.47 mM KH2PO4; pH 7.4). The sample was fixed with 4 % (v/v) paraformaldehyd ...
Specific Innervation of Neurons in the Paravertebral
... was removed and immersed in fresh fixative for an additional 2 hr. Thereafter, it was rinsed in phosphate buffer, equilibrated in buffered 30% sucrose, and embedded in gelatin-albumin (Rubin, 1985a). Serial transverse or horizontal sections, 30 pm, were made using a cryostat; these were then mounted ...
... was removed and immersed in fresh fixative for an additional 2 hr. Thereafter, it was rinsed in phosphate buffer, equilibrated in buffered 30% sucrose, and embedded in gelatin-albumin (Rubin, 1985a). Serial transverse or horizontal sections, 30 pm, were made using a cryostat; these were then mounted ...
... PDE inhibitors were compared. Corresponding to isoenzyme analysis, it was demonstrated that both PDE III and PDE IV have to be inhibited for complete suppression of either tumour necrosis factor-α (TNF-α) release from macrophages, or lymphocyte proliferation (PDE III/IV cells). In eosinophils (PDE I ...
The role of the NKG2D receptor for tumor immunity
... The NKG2D receptor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.1. The NKG2D receptor complex . . . . . . . . . . . . . . . . . . . . . . . . . ...
... The NKG2D receptor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.1. The NKG2D receptor complex . . . . . . . . . . . . . . . . . . . . . . . . . ...
9. Fibrous proteins and collagen
... Each fibrous protein exhibits special mechanical properties, resulting from its unique structure, which are obtained by combining specific amino acids into regular, secondary structural elements. This is in contrast to globular proteins, whose shapes are the result of complex interactions between se ...
... Each fibrous protein exhibits special mechanical properties, resulting from its unique structure, which are obtained by combining specific amino acids into regular, secondary structural elements. This is in contrast to globular proteins, whose shapes are the result of complex interactions between se ...
Novel Mechanism of PTEN Regulation by Its
... PTEN have been shown to be required for PI(3,4,5)P3 production and chemotaxis toward cAMP (11, 12). In wild type Dictyostelium cells, the level of PI(3,4,5)P3 transiently increases within 5 s upon cAMP stimulation and returns to basal levels within 60 s. However, in mutants lacking two of three PI3K ...
... PTEN have been shown to be required for PI(3,4,5)P3 production and chemotaxis toward cAMP (11, 12). In wild type Dictyostelium cells, the level of PI(3,4,5)P3 transiently increases within 5 s upon cAMP stimulation and returns to basal levels within 60 s. However, in mutants lacking two of three PI3K ...
The maxillary recess of the sphenoid sinus
... lateral nasal wall, axial and sagittal views can provide important additional information [6]. Earlier, Arslan H et al., also by the use of CT, evaluated that infraorbital ethmoid cells (Haller’ cells) are present in 6% only [7]. Due to this important difference between the frequency of the Haller c ...
... lateral nasal wall, axial and sagittal views can provide important additional information [6]. Earlier, Arslan H et al., also by the use of CT, evaluated that infraorbital ethmoid cells (Haller’ cells) are present in 6% only [7]. Due to this important difference between the frequency of the Haller c ...
Functions of mammalian microRNA in innate immunity to microbial
... induction of miR-155, while bypassing the negative control by miR-146. This becomes relevant during the infection of macrophages with facultative intracellular bacteria such ...
... induction of miR-155, while bypassing the negative control by miR-146. This becomes relevant during the infection of macrophages with facultative intracellular bacteria such ...
Electron Microscopy of Intermediate Filaments: Teaming up with
... 1. Glycerol Spraying/Low-Angle Rotary Metal Shadowing of Vimentin Tetramers Vimentin, recombinantly expressed in bacteria, is easily purified from inclusion bodies after dissolution with 8 M urea and column chromatography (Herrmann et al., 2004; Strelkov et al., 2004). The protein can then be stored ...
... 1. Glycerol Spraying/Low-Angle Rotary Metal Shadowing of Vimentin Tetramers Vimentin, recombinantly expressed in bacteria, is easily purified from inclusion bodies after dissolution with 8 M urea and column chromatography (Herrmann et al., 2004; Strelkov et al., 2004). The protein can then be stored ...
Supplementary Information
... The C47B161 paratope is composed of residues from all CDRs except CDR-L3 (Supplemental Figure 4). In comparison to the other anti-CD47 Fabs under evaluation, C47B161 has a five-residue-longer CDR-L1. Residues Y35 and Y37 are near the tip of the CDR-L1 loop and enhance the affinity of the antibody fo ...
... The C47B161 paratope is composed of residues from all CDRs except CDR-L3 (Supplemental Figure 4). In comparison to the other anti-CD47 Fabs under evaluation, C47B161 has a five-residue-longer CDR-L1. Residues Y35 and Y37 are near the tip of the CDR-L1 loop and enhance the affinity of the antibody fo ...
Hox genes, neural crest cells and branchial arch patterning Paul A
... The cranial neural crest is a pluripotent, mesenchymal population that plays a critical role in construction of the vertebrate head. Arising at the junction between the neural plate and surface ectoderm, cranial neural crest cells form nerve, ganglia, cartilage, bone and connective tissue. Many cran ...
... The cranial neural crest is a pluripotent, mesenchymal population that plays a critical role in construction of the vertebrate head. Arising at the junction between the neural plate and surface ectoderm, cranial neural crest cells form nerve, ganglia, cartilage, bone and connective tissue. Many cran ...
THE MOLECULAR PROFILE OF ORAL PLASMABLASTIC
... differentiation defined by the presence of more mature plasmacytic cells intermingled with the blasts in the background. ...
... differentiation defined by the presence of more mature plasmacytic cells intermingled with the blasts in the background. ...
A Phosphorylation State-specific Antibody Recognizes Hsp27, a
... PKD,1 originally cloned and termed PKC! and identified as a PKC (protein kinase C) family member, comprises a family of three closely related isoforms, PKD1, PKD2, and PKD3/PKC". Based on sequence similarities, PKDs are now grouped into the CAMK (calcium and calmodulin-dependent kinases) family of k ...
... PKD,1 originally cloned and termed PKC! and identified as a PKC (protein kinase C) family member, comprises a family of three closely related isoforms, PKD1, PKD2, and PKD3/PKC". Based on sequence similarities, PKDs are now grouped into the CAMK (calcium and calmodulin-dependent kinases) family of k ...
Lack of correlation between trehalose accumulation, cell viability
... disaccharide synthesis could be attributed t o a twofold higher trehalose-6phosphate synthase activity, together with a fourfold higher content of intracellular UDP-Glc. In addition, this mutant showed 1.5-fold higher levels of ATP compared to the wild-type. The various stress treatments studied sho ...
... disaccharide synthesis could be attributed t o a twofold higher trehalose-6phosphate synthase activity, together with a fourfold higher content of intracellular UDP-Glc. In addition, this mutant showed 1.5-fold higher levels of ATP compared to the wild-type. The various stress treatments studied sho ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.