Microtubules Contribute to Tubule Elongation and
... slower rate than the actin filament-based ER extension. Treatment with the actin-depolymerizing drug Latrunculin B made it possible to visualize the slow extension of the ER tubules in transgenic Arabidopsis (Arabidopsis thaliana) plants expressing ER-targeted green fluorescent protein. The ER tubules ...
... slower rate than the actin filament-based ER extension. Treatment with the actin-depolymerizing drug Latrunculin B made it possible to visualize the slow extension of the ER tubules in transgenic Arabidopsis (Arabidopsis thaliana) plants expressing ER-targeted green fluorescent protein. The ER tubules ...
Microbiology Focus 6.1 - The Role of - Sigma
... intracellular persistence of MRSA also occurs in human infections. This is a subject of intense debate which is also difficult to assess experimentally. In addition to this problem, antibiotic therapy for MRSA infections is becoming more difficult in hospitals because of strong biofilm-forming prope ...
... intracellular persistence of MRSA also occurs in human infections. This is a subject of intense debate which is also difficult to assess experimentally. In addition to this problem, antibiotic therapy for MRSA infections is becoming more difficult in hospitals because of strong biofilm-forming prope ...
as a PDF
... considerable variability between patients in clinical response to glucocorticoid treatment. The pharmacokinetics and pharmacodynamics of glucocorticoids have therefore been evaluated in many studies. Generally, the indications for glucocorticoids can be divided into two categories. The first categor ...
... considerable variability between patients in clinical response to glucocorticoid treatment. The pharmacokinetics and pharmacodynamics of glucocorticoids have therefore been evaluated in many studies. Generally, the indications for glucocorticoids can be divided into two categories. The first categor ...
Aprotinin Preserves Cellular Junctions and Reduces
... cellular junctions. Aprotinin has been demonstrated to prevent activation of the thrombin protease-activated receptor, and we hypothesized that aprotinin preserves myocardial cellular junctions and prevents myocardial edema in a porcine model of regional ischemia and cardioplegic arrest. Methods and ...
... cellular junctions. Aprotinin has been demonstrated to prevent activation of the thrombin protease-activated receptor, and we hypothesized that aprotinin preserves myocardial cellular junctions and prevents myocardial edema in a porcine model of regional ischemia and cardioplegic arrest. Methods and ...
Gain and Loss of Photosynthetic Membranes during
... null nor uniform with respect to thylakoid maturation. We also found that, depending on their position within the SAM, plastids undergo different developmental processes that can lead to either acquisition or loss of thylakoid membranes. These processes are not restricted to the SAM, but continue al ...
... null nor uniform with respect to thylakoid maturation. We also found that, depending on their position within the SAM, plastids undergo different developmental processes that can lead to either acquisition or loss of thylakoid membranes. These processes are not restricted to the SAM, but continue al ...
Space to grow: interplay between growth and patterning in plant
... Figure 1. Pattern emergence from auxin maxima in plants. (A) Schematic representation of the plant model organism Arabidopsis thaliana showing zones (highlighted red and whose names are written next to them) where auxin accumulation provides positional information for organ patterning. High auxin co ...
... Figure 1. Pattern emergence from auxin maxima in plants. (A) Schematic representation of the plant model organism Arabidopsis thaliana showing zones (highlighted red and whose names are written next to them) where auxin accumulation provides positional information for organ patterning. High auxin co ...
Increased Agonist Affinity at the
... (derm A594), to MORs was measured in live cells. The affinity of derm A594 binding increased after prolonged treatment of cells with multiple agonists that are known to cause receptor desensitization. In contrast, binding of a fluorescent antagonist, naltrexamine Alexa594, was unaffected by similar ...
... (derm A594), to MORs was measured in live cells. The affinity of derm A594 binding increased after prolonged treatment of cells with multiple agonists that are known to cause receptor desensitization. In contrast, binding of a fluorescent antagonist, naltrexamine Alexa594, was unaffected by similar ...
Chediak-Higashi–Like Granules in Acute
... powerful tool for better understanding of happenings inside cells, and showing different processes affecting blood and bone marrow cells. Two years ago Blood honored my devotion to this morphology by publishing one picture of a cytoplasmic bridge between basophilic normoblasts. (Delayed Cytokinesis ...
... powerful tool for better understanding of happenings inside cells, and showing different processes affecting blood and bone marrow cells. Two years ago Blood honored my devotion to this morphology by publishing one picture of a cytoplasmic bridge between basophilic normoblasts. (Delayed Cytokinesis ...
Synaptic-Type a1b2g2L GABA A Receptors Produce Large
... 50 mM GABA. Sample currents are shown in Fig. 1, A and B. The anesthetic compound propofol potentiates currents from a1b2g2L receptors activated by submaximal concentrations of GABA (Ruesch et al., 2012). We found that coapplication of 1 mM propofol with 0.5 mM GABA potentiated the peak response by ...
... 50 mM GABA. Sample currents are shown in Fig. 1, A and B. The anesthetic compound propofol potentiates currents from a1b2g2L receptors activated by submaximal concentrations of GABA (Ruesch et al., 2012). We found that coapplication of 1 mM propofol with 0.5 mM GABA potentiated the peak response by ...
PDF - UNT Digital Library
... concentration. Each point is the mean F S.E.M. for five to six cells. The data were fitted to a Hill equation to give the following IC50 and nH: 103 AM, 0.77 at 0 mV; 69 AM, 0.82 at + 40 mV; 45 AM, 0.85 at + 70 mV, respectively. (B) Voltage dependence of current inhibition by fluoxetine. The smoothe ...
... concentration. Each point is the mean F S.E.M. for five to six cells. The data were fitted to a Hill equation to give the following IC50 and nH: 103 AM, 0.77 at 0 mV; 69 AM, 0.82 at + 40 mV; 45 AM, 0.85 at + 70 mV, respectively. (B) Voltage dependence of current inhibition by fluoxetine. The smoothe ...
Live cell imaging reveals actin-cytoskeleton
... which each consist of a double zinc finger motif, display intrinsic actin-binding activity in vitro (Thomas et al., 2007). It is therefore possible that plant LIMs crosslink actin filaments in a monomeric form, although dimerization or oligomerization cannot be ruled out. Indeed, a recent study focu ...
... which each consist of a double zinc finger motif, display intrinsic actin-binding activity in vitro (Thomas et al., 2007). It is therefore possible that plant LIMs crosslink actin filaments in a monomeric form, although dimerization or oligomerization cannot be ruled out. Indeed, a recent study focu ...
Frequently Asked Questions
... 8. How does the drug release mechanism from Carbopol® polymer matrix tablets compare with that of linear, hydrophilic polymers (hypromellose)? The drug release mechanism from Carbopol® polymers and linear, (soluble) hydrophilic polymers is similar in that both are forming hydrophilic matrices. In t ...
... 8. How does the drug release mechanism from Carbopol® polymer matrix tablets compare with that of linear, hydrophilic polymers (hypromellose)? The drug release mechanism from Carbopol® polymers and linear, (soluble) hydrophilic polymers is similar in that both are forming hydrophilic matrices. In t ...
poly(lactic-co-glycolic acid) based drug delivery devices
... of biodegradable polymeric materials have been already used in this field. A wide variety of natural and synthetic biodegradable polymers have been investigated in this field. Naturally derived polymers have the potential advantage of biological recognition that may positively support cell adhesion ...
... of biodegradable polymeric materials have been already used in this field. A wide variety of natural and synthetic biodegradable polymers have been investigated in this field. Naturally derived polymers have the potential advantage of biological recognition that may positively support cell adhesion ...
The Wnt signaling pathway mechanisms
... tube as a result of local differentiation and proliferation of cardiomyocytes (de Jong et al., 1997; Christoffels et al., 2000; Christoffels et al., 2004). With development the heart tube increases in length by the addition of cardiomyocytes at both the arterial and venous poles of the heart tube ra ...
... tube as a result of local differentiation and proliferation of cardiomyocytes (de Jong et al., 1997; Christoffels et al., 2000; Christoffels et al., 2004). With development the heart tube increases in length by the addition of cardiomyocytes at both the arterial and venous poles of the heart tube ra ...
Checking Drug Sensitivity of Cell Lines Used in Signatures
... The fact that daunorubicin and epirubicin exhibit similar response patterns to doxorubicin but vincristine does not is not surprising, as the first (Dauno, Epi, D) share a common mechanism of action and the last (Vinc) does not. The lack of agreement between doxorubicin and vincristine is troubling ...
... The fact that daunorubicin and epirubicin exhibit similar response patterns to doxorubicin but vincristine does not is not surprising, as the first (Dauno, Epi, D) share a common mechanism of action and the last (Vinc) does not. The lack of agreement between doxorubicin and vincristine is troubling ...
Full-Text PDF
... a substrate “by spinning a mat of polysaccharide fibers that withstands enormous shear forces . . . on the surface of sedimentary rocks in mountain streams, the “glycocalyx” prevents washing out of the attached bacteria” and provides a conveyor band-similar situation “in which the water flow supplie ...
... a substrate “by spinning a mat of polysaccharide fibers that withstands enormous shear forces . . . on the surface of sedimentary rocks in mountain streams, the “glycocalyx” prevents washing out of the attached bacteria” and provides a conveyor band-similar situation “in which the water flow supplie ...
Carbon partitioning in sugarcane (Saccharum species)
... compartmentation of sugars between the apoplast, cytosol, and vacuole is an important feature of storage in sugarcane stem’s parenchyma cells (Rae et al., 2009). Sucrose unloaded from phloem into the apoplast can follow two paths to vacuoles of parenchyma cells (Figure 1). In one path, sucrose is tr ...
... compartmentation of sugars between the apoplast, cytosol, and vacuole is an important feature of storage in sugarcane stem’s parenchyma cells (Rae et al., 2009). Sucrose unloaded from phloem into the apoplast can follow two paths to vacuoles of parenchyma cells (Figure 1). In one path, sucrose is tr ...
Mitochondrial Antiviral Signaling Protein Innate Immunity by
... postinfection and were then transfected with poly(dAT:dAT) together with IFN-b–Luc, NF-kB–Luc, or IRF-3–Luc. Serum from uninfected individuals was used as a control. The Luc activity was measured 24 h later and normalized for transfection efficiency. C, HepG2-1117 cells were infected with VSV with o ...
... postinfection and were then transfected with poly(dAT:dAT) together with IFN-b–Luc, NF-kB–Luc, or IRF-3–Luc. Serum from uninfected individuals was used as a control. The Luc activity was measured 24 h later and normalized for transfection efficiency. C, HepG2-1117 cells were infected with VSV with o ...
Auxin and other signals on the move in plants
... root and epidermis patterning, respectively. These proteins are cytosolic and inactive in the cells in which they are produced, but after they have moved to neighboring cells through plasmodesmata, they are targeted to the nucleus and become active in promoting transcription of patterning factors44, ...
... root and epidermis patterning, respectively. These proteins are cytosolic and inactive in the cells in which they are produced, but after they have moved to neighboring cells through plasmodesmata, they are targeted to the nucleus and become active in promoting transcription of patterning factors44, ...
The GDP-bound form of Arf6 is located at the plasma membrane
... conserved residue in the phosphate-binding P-loop is broadly used to investigate the cellular function of the small G proteins. This mutation generally, but not always, yields a protein with a dominant-negative cellular activity that can be explained biochemically by the formation of a nucleotide-fr ...
... conserved residue in the phosphate-binding P-loop is broadly used to investigate the cellular function of the small G proteins. This mutation generally, but not always, yields a protein with a dominant-negative cellular activity that can be explained biochemically by the formation of a nucleotide-fr ...
Polyethyleneimine is an effective permeabilizer of Gram
... Briefly, the bacteria were cultivated to OD,,, 0-5,then the cells were deposited by centrifugation (1000 g, 10 min at room temperature) and suspended in a similar volume of 5 mM HEPES, pH 7-2. Samples (1.35ml) of this suspension were pipetted directly into the fluorometer cuvette, and fluorescence w ...
... Briefly, the bacteria were cultivated to OD,,, 0-5,then the cells were deposited by centrifugation (1000 g, 10 min at room temperature) and suspended in a similar volume of 5 mM HEPES, pH 7-2. Samples (1.35ml) of this suspension were pipetted directly into the fluorometer cuvette, and fluorescence w ...
Immune Escape of Human Papillomavirus Type 16 in A Major Role
... formation of the virion. Furthermore, L2 facilitates HPV infection through an interaction between the N terminus region of the L2 protein and an unknown cell surface receptor (11, 12). Further functions of L2 include binding of the virion to the cytoskeleton, transport within the cytoplasm (13), and ...
... formation of the virion. Furthermore, L2 facilitates HPV infection through an interaction between the N terminus region of the L2 protein and an unknown cell surface receptor (11, 12). Further functions of L2 include binding of the virion to the cytoskeleton, transport within the cytoplasm (13), and ...
Dev Biol 364(2), 138-48. PDF
... 2008; Zallen, 2007). The signaling pathway that controls PCP consists of Celsr/Flamingo, Frizzled (Fzd), Disheveled (Dvl/Dsh), Van Gogh/strabisumus (Vang/stbm), Diego, and Prickle (Pk). The PCP pathway is proposed to modulate the cytoskeleton and influence cell morphology rather than cell fates in ma ...
... 2008; Zallen, 2007). The signaling pathway that controls PCP consists of Celsr/Flamingo, Frizzled (Fzd), Disheveled (Dvl/Dsh), Van Gogh/strabisumus (Vang/stbm), Diego, and Prickle (Pk). The PCP pathway is proposed to modulate the cytoskeleton and influence cell morphology rather than cell fates in ma ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.