DIFFERENT MODES OF FLUORESCENTLY LABELED
... 2Laboratory of Neuroendocrinology-Molecular Cell Physiology, Institute of Pathophysiology, Medical Faculty, University of Ljubljana, Zaloska 4, SI-1000 Ljubljana, Slovenia. Introduction. Astrocytes, the most abundant type of glial cells in the central nervous system, play an important role in synapt ...
... 2Laboratory of Neuroendocrinology-Molecular Cell Physiology, Institute of Pathophysiology, Medical Faculty, University of Ljubljana, Zaloska 4, SI-1000 Ljubljana, Slovenia. Introduction. Astrocytes, the most abundant type of glial cells in the central nervous system, play an important role in synapt ...
... The mechanisms that control protein transport between the endoplasmic reticulum (ER) and the Golgi apparatus are poorly characterized in plants. Here, we examine in tobacco leaves the structural relationship between Golgi and ER membranes using electron microscopy and demonstrate that Golgi membrane ...
The PDZ-GEF Dizzy regulates cell shape of migrating macrophages
... adhesion then provide traction during the contraction of the cell, leading to the forward movement of the cell body. At the rear of the cell, adhesion sites become disassembled, allowing the cell to detach and to efficiently migrate toward guidance cues (Friedl and Wolf, 2003; Lauffenburger and Horw ...
... adhesion then provide traction during the contraction of the cell, leading to the forward movement of the cell body. At the rear of the cell, adhesion sites become disassembled, allowing the cell to detach and to efficiently migrate toward guidance cues (Friedl and Wolf, 2003; Lauffenburger and Horw ...
LVA and HVA Ca Currents in Ventricular Muscle Cells of the
... Differential effects of the two ions on surface charge screening would normally predict a shift to the left as shown for the voltage step protocols in Fig. 5, and thus we can only assume that this is an artifact seen solely during voltage ramp protocols. The observation that the amplitude of the HVA ...
... Differential effects of the two ions on surface charge screening would normally predict a shift to the left as shown for the voltage step protocols in Fig. 5, and thus we can only assume that this is an artifact seen solely during voltage ramp protocols. The observation that the amplitude of the HVA ...
CYTOKINE-MEDIATED REGULATION OF BK VIRUS REPLICATION
... BKV occurs in immunocompromised individuals and can lead to severe disease. BKVassociated diseases include polyomavirus nephropathy (PVN), a form of acute interstitial nephritis that afflicts up to 10% of renal transplant recipients, and hemorrhagic cystitis (HC), an infection of the bladder charact ...
... BKV occurs in immunocompromised individuals and can lead to severe disease. BKVassociated diseases include polyomavirus nephropathy (PVN), a form of acute interstitial nephritis that afflicts up to 10% of renal transplant recipients, and hemorrhagic cystitis (HC), an infection of the bladder charact ...
Endoplasmic Reticulum Export Sites and Golgi Bodies Behave as
... characterized. A widely accepted model for ER-to-Golgi transport is based on the sequential action of COPII and COPI coat complexes. The COPII complex assembles by the ordered recruitment of cytosolic components on the ER membrane. Here, we have visualized two early components of the COPII machinery ...
... characterized. A widely accepted model for ER-to-Golgi transport is based on the sequential action of COPII and COPI coat complexes. The COPII complex assembles by the ordered recruitment of cytosolic components on the ER membrane. Here, we have visualized two early components of the COPII machinery ...
2. introduction
... 240msec. The 6th cell from the top of the image developed a spontaneous Ca2+ wave that cascaded to the top 5 cells in succession and to one additional cell below where it was extinguished. B: higher magnification of the image (from red line to the right of the image in A) showing Ca2+ spritzes (red ...
... 240msec. The 6th cell from the top of the image developed a spontaneous Ca2+ wave that cascaded to the top 5 cells in succession and to one additional cell below where it was extinguished. B: higher magnification of the image (from red line to the right of the image in A) showing Ca2+ spritzes (red ...
Encapsulation of Plasmid DNA in Stabilized Plasmid – Lipid
... Huang, 1997). Furthermore, these complexes often exhibit limited stability, have a tendency for aggregation and exhibit transfection potencies that can be sample and time dependent. Methods for passive encapsulation of plasmid into liposomes are highly inefficient and result in low DNA/lipid ratios ...
... Huang, 1997). Furthermore, these complexes often exhibit limited stability, have a tendency for aggregation and exhibit transfection potencies that can be sample and time dependent. Methods for passive encapsulation of plasmid into liposomes are highly inefficient and result in low DNA/lipid ratios ...
Characterization of the Nucleolar Gene Product, Treacle, in Treacher Collins Syndrome
... treacle band on SDS-PAGE, we in vitro transcribed/translated treacle and analyzed it by SDS-PAGE and fluorography. For this purpose, we used the full-length cDNA of mouse treacle, which is 61.5% identical to human treacle (Dixon et al., 1997b; Paznekas et al., 1997). Despite being 109 amino acids sh ...
... treacle band on SDS-PAGE, we in vitro transcribed/translated treacle and analyzed it by SDS-PAGE and fluorography. For this purpose, we used the full-length cDNA of mouse treacle, which is 61.5% identical to human treacle (Dixon et al., 1997b; Paznekas et al., 1997). Despite being 109 amino acids sh ...
Spirulina – Immune System Booster
... immunostimulating effect by enhancing – the resistance to infections – the capacity of influencing hemopoieses – stimulating the production of antibodies and cytokines ...
... immunostimulating effect by enhancing – the resistance to infections – the capacity of influencing hemopoieses – stimulating the production of antibodies and cytokines ...
Separation of Bacteria, Protozoa and Carbon Nanotubes by Density
... microscopy (ESEM) images (Figure S1). MWCNTs appeared less tangled after probe-sonication which is expected based on the known effects of high-energy probe-sonication processes on MWCNTs [27]. Based on XPS performed previously [26], 6-h probe-sonication also increased the surface oxygen content of M ...
... microscopy (ESEM) images (Figure S1). MWCNTs appeared less tangled after probe-sonication which is expected based on the known effects of high-energy probe-sonication processes on MWCNTs [27]. Based on XPS performed previously [26], 6-h probe-sonication also increased the surface oxygen content of M ...
Altered & Disordered Physiology - CH 056
... Unconjugated Bilirubin • Bilirubin, bound onto albumin, prior to processing in the liver is often referred to as ‘unconjugated’. • Unconjugated bilirubin, bound onto albumin, is non-toxic and remains in solution for its transportation, in the blood, to the liver for processing. • A small amount, 20 ...
... Unconjugated Bilirubin • Bilirubin, bound onto albumin, prior to processing in the liver is often referred to as ‘unconjugated’. • Unconjugated bilirubin, bound onto albumin, is non-toxic and remains in solution for its transportation, in the blood, to the liver for processing. • A small amount, 20 ...
The ultrastructure of a doliolid and a copepod
... analyses, pellets of gonozooids of Dolioletta gegenbauri and females of the copepod Eucalanus pileatus were produced in feeding experiments at close to environmental food concentrations. Thin sections of a representative doliolid pellet revealed that these pellets were mainly composed of intact diat ...
... analyses, pellets of gonozooids of Dolioletta gegenbauri and females of the copepod Eucalanus pileatus were produced in feeding experiments at close to environmental food concentrations. Thin sections of a representative doliolid pellet revealed that these pellets were mainly composed of intact diat ...
Physiological assembly and activity of human
... been found to harbor active telomerase (Shay and Wright, 2005). In the pioneering characterization of human telomerase activity in vitro, DNA synthesis was assayed by monitoring the extension of oligonucleotide primers with deoxynucleotide triphosphates required to generate TTAGGG repeats (Morin, 19 ...
... been found to harbor active telomerase (Shay and Wright, 2005). In the pioneering characterization of human telomerase activity in vitro, DNA synthesis was assayed by monitoring the extension of oligonucleotide primers with deoxynucleotide triphosphates required to generate TTAGGG repeats (Morin, 19 ...
Detection, Characterization, and Bioavailability of Membrane
... It is hypothesized that membrane-associated iron in the sickle red cell is of pathophysiologic importance, but the actual existence of such iron in the intact cell has been questioned. Using a strategy whereby membrane iron can be detected through its bioavailability for catalyzing peroxidation, we ...
... It is hypothesized that membrane-associated iron in the sickle red cell is of pathophysiologic importance, but the actual existence of such iron in the intact cell has been questioned. Using a strategy whereby membrane iron can be detected through its bioavailability for catalyzing peroxidation, we ...
Polycystin-1 maturation requires polycystin-2 in a dose
... II that blocks mature (post-ER) glycosylation (52), affected only PC1-NTR, not PC1-FL, PC1-NTS, or PC2 (Figure 1, C and D, and Supplemental Figure 1F). Combined with the deglycosylation analyses, these data demonstrate that in the ER, PC2 complexes with PC1-FL and PC1-CTS, as well as PC1-NTS via PC1 ...
... II that blocks mature (post-ER) glycosylation (52), affected only PC1-NTR, not PC1-FL, PC1-NTS, or PC2 (Figure 1, C and D, and Supplemental Figure 1F). Combined with the deglycosylation analyses, these data demonstrate that in the ER, PC2 complexes with PC1-FL and PC1-CTS, as well as PC1-NTS via PC1 ...
Antiangiogenic Activity of N-substituted and
... 250 l of Matrigel (Becton-Dickinson, Bedford, MA) and allowed to gel for 30 min at 37°C and 5% CO2. Thoracic aortas were excised from 8- to 10-week-old male Sprague Dawley rats. After careful removal of fibroadipose tissues, the aortas were cut into 1-mm-long cross-sections, placed on Matrigel-coat ...
... 250 l of Matrigel (Becton-Dickinson, Bedford, MA) and allowed to gel for 30 min at 37°C and 5% CO2. Thoracic aortas were excised from 8- to 10-week-old male Sprague Dawley rats. After careful removal of fibroadipose tissues, the aortas were cut into 1-mm-long cross-sections, placed on Matrigel-coat ...
Neomycin - Australian Pesticides and Veterinary Medicines Authority
... either parenteral or oral formulations increases if the individual has compromised renal function, gastrointestinal inflammation or is receiving other potentially nephrotoxic drugs concomitantly. A close examination of the published and unpublished information for food-producing animals suggests tha ...
... either parenteral or oral formulations increases if the individual has compromised renal function, gastrointestinal inflammation or is receiving other potentially nephrotoxic drugs concomitantly. A close examination of the published and unpublished information for food-producing animals suggests tha ...
Dramatic increase in lymph node dendritic cell
... A DC-enriched cell fraction was then obtained after depletion of T cells, B cells, and granulocytes by treating the 1.061-density fraction for 50 minutes at 4°C with a monoclonal antibody (mAb) mixture including anti-CD3 (clone KT3-1.1), anti-B220 (clone RA3-6B2), and antigranulocyte antigen Gr1 (cl ...
... A DC-enriched cell fraction was then obtained after depletion of T cells, B cells, and granulocytes by treating the 1.061-density fraction for 50 minutes at 4°C with a monoclonal antibody (mAb) mixture including anti-CD3 (clone KT3-1.1), anti-B220 (clone RA3-6B2), and antigranulocyte antigen Gr1 (cl ...
p62/SQSTM1 Binds Directly to Atg8/LC3 to Facilitate Degradation of
... protein aggregates (15). These results suggest that it is the basal constitutive autophagy that is needed in order to avoid accumulation of ubiquitinated protein aggregates (13, 14, 16). Recently, the term ALIS (aggresome-like induced structures) was used to describe ubiquitin-containing bodies indu ...
... protein aggregates (15). These results suggest that it is the basal constitutive autophagy that is needed in order to avoid accumulation of ubiquitinated protein aggregates (13, 14, 16). Recently, the term ALIS (aggresome-like induced structures) was used to describe ubiquitin-containing bodies indu ...
Development of the lymphatic system: new questions and paradigms
... various knockout (KO) and knockdown (KD) studies carried out in mice and zebrafish (Table 1) have identified some of the key transcription factors that play a role in specifying cells towards a lymphatic fate. Prox1 ( prospero-related homeobox gene 1), which encodes a homeodomain transcription facto ...
... various knockout (KO) and knockdown (KD) studies carried out in mice and zebrafish (Table 1) have identified some of the key transcription factors that play a role in specifying cells towards a lymphatic fate. Prox1 ( prospero-related homeobox gene 1), which encodes a homeodomain transcription facto ...
Cell regulation by the Apc protein Apc as master regulator of epithelia
... proliferative compartment. It also contributes to apoptosis, and can enhance or inhibit cell death depending on the cellular context (position, tissue type etc.). (b) In the absence of Apc, or when only mutated Apc is present, cells do not differentiate normally; they remain proliferative and retain ...
... proliferative compartment. It also contributes to apoptosis, and can enhance or inhibit cell death depending on the cellular context (position, tissue type etc.). (b) In the absence of Apc, or when only mutated Apc is present, cells do not differentiate normally; they remain proliferative and retain ...
Mitochondria Buffer Physiological Calcium
... in HEPES-buffered Hank’s balanced salt solution (HHSS), pH 7.45, containing 0.5% bovine serum albumin. HHSS was composed of the following (in mM): HEPES, 20; NaCl, 137; CaCI,, 1.3; MgSO,, 0.4; MgCl, 0.5; KCI, 5.4; KH?PO,, 0.4; NaHPO,, 0.3; NaHCO,, 3.0; and glucose, 5.6. Loaded cells were mounted in ...
... in HEPES-buffered Hank’s balanced salt solution (HHSS), pH 7.45, containing 0.5% bovine serum albumin. HHSS was composed of the following (in mM): HEPES, 20; NaCl, 137; CaCI,, 1.3; MgSO,, 0.4; MgCl, 0.5; KCI, 5.4; KH?PO,, 0.4; NaHPO,, 0.3; NaHCO,, 3.0; and glucose, 5.6. Loaded cells were mounted in ...
Receptor Regulation of the Volume-Sensitive Efflux of Taurine and
... involvement of a VSOAC in response to hypotonic stress comes from studies in which RVD, volume-sensitive Cl⫺ current, and organic osmolyte release can all be blocked by broad-spectrum anion channel inhibitors, such as DDF or NPPB, and by a highly selective agent, DCPIB (Decher et al., 2001; Abdullae ...
... involvement of a VSOAC in response to hypotonic stress comes from studies in which RVD, volume-sensitive Cl⫺ current, and organic osmolyte release can all be blocked by broad-spectrum anion channel inhibitors, such as DDF or NPPB, and by a highly selective agent, DCPIB (Decher et al., 2001; Abdullae ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.