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... The genetic control of mammalian epithelial polarity and dynamics can be studied in vivo at cellular resolution during morphogenesis of the mouse neural tube. The mouse neural plate is a simple epithelium that is transformed into a columnar pseudostratified tube over the course of ∼24 h. Apical F-ac ...
... The genetic control of mammalian epithelial polarity and dynamics can be studied in vivo at cellular resolution during morphogenesis of the mouse neural tube. The mouse neural plate is a simple epithelium that is transformed into a columnar pseudostratified tube over the course of ∼24 h. Apical F-ac ...
Engineering microfluidic concentration gradient generators for
... ( ⃗⃗ ), is obtained from solving the previously introduced N-S convective term, equation for convective transport of solute species ( see Table I). The net rate of species formation, Rg, is usually present when there is a chemical reaction producing or consuming the solute species. This factor is om ...
... ( ⃗⃗ ), is obtained from solving the previously introduced N-S convective term, equation for convective transport of solute species ( see Table I). The net rate of species formation, Rg, is usually present when there is a chemical reaction producing or consuming the solute species. This factor is om ...
A Novel Form of DAP5 Protein Accumulates in Apoptotic Cells as a
... the other hand suggest that further studies of this gene should highlight novel mechanisms linking translational control to restriction of cellular outgrowth by PCD (21). While DAP5 was reported to be ubiquitously and abundantly expressed in normal tissues and growing cell lines (15, 21, 33, 40), th ...
... the other hand suggest that further studies of this gene should highlight novel mechanisms linking translational control to restriction of cellular outgrowth by PCD (21). While DAP5 was reported to be ubiquitously and abundantly expressed in normal tissues and growing cell lines (15, 21, 33, 40), th ...
Flamingo controls the planar polarity of sensory
... apterous–Gal4 > UAS–fmi pupae (n = 26), the Numb crescent was also mispositioned and the mitotic spindle misoriented within the epithelial plane, but they remained aligned with each other (Figure 3g,h). Therefore, loss of function and overexpression of fmi both disrupted the cellular process that re ...
... apterous–Gal4 > UAS–fmi pupae (n = 26), the Numb crescent was also mispositioned and the mitotic spindle misoriented within the epithelial plane, but they remained aligned with each other (Figure 3g,h). Therefore, loss of function and overexpression of fmi both disrupted the cellular process that re ...
Structural Sterols Are Involved in Both the Initiation and Tip Growth
... In general, the production rate of sterols varies in different cell types. Thus, it is high in proliferating and actively growing tissues, while it declines in mature tissues (Hartmann, 2004). All steps of the biosynthetic pathway of free phytosterols (Benveniste, 1986) as well as the genes responsi ...
... In general, the production rate of sterols varies in different cell types. Thus, it is high in proliferating and actively growing tissues, while it declines in mature tissues (Hartmann, 2004). All steps of the biosynthetic pathway of free phytosterols (Benveniste, 1986) as well as the genes responsi ...
Immunoglobulin free light chains in inflammatory diseases
... found to be remarkably antigen (Ag) specific compared to another similar acting heterologous specific factor from immune cells of oxazolone sensitized mice (OX-F) F had Ag-specificity in ear swelling ...
... found to be remarkably antigen (Ag) specific compared to another similar acting heterologous specific factor from immune cells of oxazolone sensitized mice (OX-F) F had Ag-specificity in ear swelling ...
Treatment of acute promyelocytic leukaemia with all
... anthracyclines which improved the complete remission (CR) rate, though the 5-year overall survival could only be attained in a small proportion of patients. A rational and intriguing hypothesis, to induce differentiation of APL cells rather than killing them, was raised in the 1970s. Laudably, the u ...
... anthracyclines which improved the complete remission (CR) rate, though the 5-year overall survival could only be attained in a small proportion of patients. A rational and intriguing hypothesis, to induce differentiation of APL cells rather than killing them, was raised in the 1970s. Laudably, the u ...
Spatial organization of the epithelium and the role of neural crest
... the dental papilla cells derived from the frog (Fig. 2). Although it is not expressed during normal larval development, the anuran neural crest has the capability both of inducing enamel organ formation and of being induced to form dentine. In a complementary experiment, urodele neural crest was gra ...
... the dental papilla cells derived from the frog (Fig. 2). Although it is not expressed during normal larval development, the anuran neural crest has the capability both of inducing enamel organ formation and of being induced to form dentine. In a complementary experiment, urodele neural crest was gra ...
Materials and Methods
... upwards into the teat canal. Milk samples were collected daily, (up to day 7), from which the SCC and viable cell numbers of S. aureus were determined. The number of replications depended on the number of available mastitis-free cows at a specific stage of lactation. Since the microbial challenge wi ...
... upwards into the teat canal. Milk samples were collected daily, (up to day 7), from which the SCC and viable cell numbers of S. aureus were determined. The number of replications depended on the number of available mastitis-free cows at a specific stage of lactation. Since the microbial challenge wi ...
OPTIMISATION OF CHO CELL GROWTH AND
... A statistical approach was used to identify important culture components for cell growth and LFN-y production. When the concentration of the resulting positive variables was initially increased in culture, improvements of approximately 40% in both of these parameters were achieved; the glycosylation ...
... A statistical approach was used to identify important culture components for cell growth and LFN-y production. When the concentration of the resulting positive variables was initially increased in culture, improvements of approximately 40% in both of these parameters were achieved; the glycosylation ...
Single Exposure of Human Oral Mucosa Fibroblasts to
... are exposed to ultraviolet (UV) sunlight. Fibroblasts are abundant resident cells of the connective tissue which are key regulators of extracellular matrix composition, as well as, epithelial and endothelial cell function. UVB light, an inherent component of sunlight, causes several alterations in s ...
... are exposed to ultraviolet (UV) sunlight. Fibroblasts are abundant resident cells of the connective tissue which are key regulators of extracellular matrix composition, as well as, epithelial and endothelial cell function. UVB light, an inherent component of sunlight, causes several alterations in s ...
Probing for Binding Regions of the FtsZ Protein Surface through Site
... We cloned each FtsZ-FP fusion into the pJSB2 plasmid, and we used the E. coli JKD7-1/pKD3 system, originally developed by Dai and Lutkenhaus (28), to test for complementation. JKD7-1 cells, which are genomic ftsZ null cells, are supported by the expression of wtFtsZ from a rescue plasmid, pKD3. pKD3 ...
... We cloned each FtsZ-FP fusion into the pJSB2 plasmid, and we used the E. coli JKD7-1/pKD3 system, originally developed by Dai and Lutkenhaus (28), to test for complementation. JKD7-1 cells, which are genomic ftsZ null cells, are supported by the expression of wtFtsZ from a rescue plasmid, pKD3. pKD3 ...
Direct binding of Nur77/NAK-1 to the plasminogen activator inhibitor
... growth factors,13,14 as well as inflammatory cytokines. Atherosclerosis is indeed regarded as a disease with a significant inflammatory component (reviewed in Libby et al15), and PAI-1 expression is up-regulated by inflammatory mediators such as tumor necrosis factor ␣ (TNF␣),16 interleukin-1  (IL- ...
... growth factors,13,14 as well as inflammatory cytokines. Atherosclerosis is indeed regarded as a disease with a significant inflammatory component (reviewed in Libby et al15), and PAI-1 expression is up-regulated by inflammatory mediators such as tumor necrosis factor ␣ (TNF␣),16 interleukin-1  (IL- ...
the article
... to serve as an ‘architectural’ protein22. As it is now appreciated that HMGB1 can be released from cells, we hypothesized that in a way analogous to its binding of nuclear DNA, HMGB1 could bind to extracellular DNA and, by forming HMGB1-DNA complexes, modify the immune-regulatory effects of specific ...
... to serve as an ‘architectural’ protein22. As it is now appreciated that HMGB1 can be released from cells, we hypothesized that in a way analogous to its binding of nuclear DNA, HMGB1 could bind to extracellular DNA and, by forming HMGB1-DNA complexes, modify the immune-regulatory effects of specific ...
Cooperation between the RING+B1-B2 and coiled-coil
... from the NBs (Le et al., 1996). However, whether the ability of PML to suppress transformation in certain experimental systems re¯ects a more general function of growth suppression and whether and how this function is lost in APL remain to be established. We report here that PML suppresses cell grow ...
... from the NBs (Le et al., 1996). However, whether the ability of PML to suppress transformation in certain experimental systems re¯ects a more general function of growth suppression and whether and how this function is lost in APL remain to be established. We report here that PML suppresses cell grow ...
Effects of nitric oxide on red blood cell deformability
... Furthermore, Mesquita et al. (29) have shown that both acetylcholine (ACh) and the NO donor spermineNONOate improve RBC deformability and suggest that the effect of ACh may be due to induced NO synthesis mediated by M1-type ACh receptors on the RBC membrane. Chronic inhibition of NOS by Nnitro-L-ar ...
... Furthermore, Mesquita et al. (29) have shown that both acetylcholine (ACh) and the NO donor spermineNONOate improve RBC deformability and suggest that the effect of ACh may be due to induced NO synthesis mediated by M1-type ACh receptors on the RBC membrane. Chronic inhibition of NOS by Nnitro-L-ar ...
Potential New Therapeutic Agents: Effects on HIV Replication and Viral Escape
... Developing new anti-HIV compounds targeting different steps of the HIV cycle is a continuous need due to the emergence of drug-resistant strains and also to the long-term toxicity of current antiretrovirals used for treatment. HIV entry requires the binding of the viral particles to the CD4 receptor ...
... Developing new anti-HIV compounds targeting different steps of the HIV cycle is a continuous need due to the emergence of drug-resistant strains and also to the long-term toxicity of current antiretrovirals used for treatment. HIV entry requires the binding of the viral particles to the CD4 receptor ...
Saccharomyces cerevisiae
... inhibitors (phenylmethane sulphonyl fluoride, pepstatin, leupeptin) either alone or in mixtures to the breakage medium did not enhance glucan synthase stability, suggesting that the enzyme was not inactivated through a proteolytic mechanism. By adding 0.2-2.0M-sucrose to the breakage medium as an os ...
... inhibitors (phenylmethane sulphonyl fluoride, pepstatin, leupeptin) either alone or in mixtures to the breakage medium did not enhance glucan synthase stability, suggesting that the enzyme was not inactivated through a proteolytic mechanism. By adding 0.2-2.0M-sucrose to the breakage medium as an os ...
catecholamine-depleted rat ventricular myocytes
... glycol brought to 20 ml with distilled water such that, to obtain the desired final concentration, it was necessary to administer 1 ml of reserpine solution per kilogram to each animal. Fresh reserpine solution was prepared daily. Control animals were either untreated or injected with vehicle only. ...
... glycol brought to 20 ml with distilled water such that, to obtain the desired final concentration, it was necessary to administer 1 ml of reserpine solution per kilogram to each animal. Fresh reserpine solution was prepared daily. Control animals were either untreated or injected with vehicle only. ...
Comparative Cell Biology and Evolution of Annexins in Diplomonads
... alpha-giardins, are the best-studied annexins assigned to group E (4). The alphagiardins were originally discovered as prominent proteins of the structurally complex Giardia intestinalis cytoskeleton and found to be immunodominant during human G. intestinalis infection (5, 6). Annexins in parasites ...
... alpha-giardins, are the best-studied annexins assigned to group E (4). The alphagiardins were originally discovered as prominent proteins of the structurally complex Giardia intestinalis cytoskeleton and found to be immunodominant during human G. intestinalis infection (5, 6). Annexins in parasites ...
Tendon development and musculoskeletal assembly: emerging
... cranial and limb muscles, the TPCs and tendon matrix determine the pattern of attachments (Kardon, 1998; Kieny and Chevallier, 1979; Noden, 1988). Do muscles and the myomatrix play any role in TPC formation or maintenance? In the limbs of chick embryos in which muscle progenitors have been surgicall ...
... cranial and limb muscles, the TPCs and tendon matrix determine the pattern of attachments (Kardon, 1998; Kieny and Chevallier, 1979; Noden, 1988). Do muscles and the myomatrix play any role in TPC formation or maintenance? In the limbs of chick embryos in which muscle progenitors have been surgicall ...
Functional Analysis of Class 1 RSL Genes in Caulonema and
... 1993). Chloronema cells are short and contain numerous chloroplasts and perpendicular cell walls, compared to caulonema. The numerous well-developed chloroplasts are very similar to those of leaf cells in ultrastructure, suggesting that the main function of chloronema cells in protonema development ...
... 1993). Chloronema cells are short and contain numerous chloroplasts and perpendicular cell walls, compared to caulonema. The numerous well-developed chloroplasts are very similar to those of leaf cells in ultrastructure, suggesting that the main function of chloronema cells in protonema development ...
Exocytosis Precedes and Predicts the Increase in
... 50 s (Pierson et al., 1996; Cárdenas et al., 2008). Additionally, many of the underlying processes also oscillate with the same period, but usually with a different phase than the growth rate (Holdaway-Clarke and Hepler, 2003; Chebli and Geitmann, 2007; Moreno et al., 2007). Thus, growth emerges as ...
... 50 s (Pierson et al., 1996; Cárdenas et al., 2008). Additionally, many of the underlying processes also oscillate with the same period, but usually with a different phase than the growth rate (Holdaway-Clarke and Hepler, 2003; Chebli and Geitmann, 2007; Moreno et al., 2007). Thus, growth emerges as ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.