Receptor Regulation of the Volume-Sensitive Efflux of Taurine and
... involvement of a VSOAC in response to hypotonic stress comes from studies in which RVD, volume-sensitive Cl⫺ current, and organic osmolyte release can all be blocked by broad-spectrum anion channel inhibitors, such as DDF or NPPB, and by a highly selective agent, DCPIB (Decher et al., 2001; Abdullae ...
... involvement of a VSOAC in response to hypotonic stress comes from studies in which RVD, volume-sensitive Cl⫺ current, and organic osmolyte release can all be blocked by broad-spectrum anion channel inhibitors, such as DDF or NPPB, and by a highly selective agent, DCPIB (Decher et al., 2001; Abdullae ...
Receptor Regulation of the Volume-Sensitive Efflux of Taurine and
... involvement of a VSOAC in response to hypotonic stress comes from studies in which RVD, volume-sensitive Cl⫺ current, and organic osmolyte release can all be blocked by broad-spectrum anion channel inhibitors, such as DDF or NPPB, and by a highly selective agent, DCPIB (Decher et al., 2001; Abdullae ...
... involvement of a VSOAC in response to hypotonic stress comes from studies in which RVD, volume-sensitive Cl⫺ current, and organic osmolyte release can all be blocked by broad-spectrum anion channel inhibitors, such as DDF or NPPB, and by a highly selective agent, DCPIB (Decher et al., 2001; Abdullae ...
Dual Modes of Endoplasmic Reticulum-to
... or just late-stage post-Golgi vesicles contribute to dendritic secretory trafficking remains poorly understood. In this study, we have used live-cell confocal imaging experiments along with immunocytochemistry in cultured hippocampal neurons to define the secretory pathway in dendrites. We demonstra ...
... or just late-stage post-Golgi vesicles contribute to dendritic secretory trafficking remains poorly understood. In this study, we have used live-cell confocal imaging experiments along with immunocytochemistry in cultured hippocampal neurons to define the secretory pathway in dendrites. We demonstra ...
Organization of cardiac chamber progenitors in the zebrafish blastula
... contributions at 44 hpf. Lateral views of the heart, anterior towards the left. Labeled myocardial (E,F,I,J) and endocardial (G,H,K,L) progeny are marked with a blue precipitate, and myocardium is counterstained with a pink precipitate (see Materials and methods). Identities of labeled cells are det ...
... contributions at 44 hpf. Lateral views of the heart, anterior towards the left. Labeled myocardial (E,F,I,J) and endocardial (G,H,K,L) progeny are marked with a blue precipitate, and myocardium is counterstained with a pink precipitate (see Materials and methods). Identities of labeled cells are det ...
Targeted wild-type and jerker espins reveal a novel, WH2
... additional attributes with well-characterized WH2 domains (see below). Deletion of the 17-aa core of the espin WH2 domain eliminated CAB formation and resulted instead in the accumulation of GFP-espin and F-actin in filopodia-like structures at the cell periphery (Fig. 1J, Fig. 3B). The segment requ ...
... additional attributes with well-characterized WH2 domains (see below). Deletion of the 17-aa core of the espin WH2 domain eliminated CAB formation and resulted instead in the accumulation of GFP-espin and F-actin in filopodia-like structures at the cell periphery (Fig. 1J, Fig. 3B). The segment requ ...
Interaction of Antiparallel Microtubules in the
... vation. At an early stage of cytokinesis, when Golgi-derived vesicles began to accumulate at the division site, long MTs were frequently detected in the phragmoplast formed between two reforming daughter nuclei (Figure 1A). Although many MTs were terminated in regions where vesicles accumulated, oth ...
... vation. At an early stage of cytokinesis, when Golgi-derived vesicles began to accumulate at the division site, long MTs were frequently detected in the phragmoplast formed between two reforming daughter nuclei (Figure 1A). Although many MTs were terminated in regions where vesicles accumulated, oth ...
insight into production, drug loading, targeting, and
... [4], [5]. In spite of these advantages of NPs, efficient drug delivery has never been fully ...
... [4], [5]. In spite of these advantages of NPs, efficient drug delivery has never been fully ...
p23 and organization of the Golgi apparatus
... between a p24 protein and a cargo molecule has been reported. However, it is possible that p24-cargo interactions are weak and/or transient, and cannot be revealed by classical biochemical approaches. Alternatively, it is possible that p24 proteins do not directly interact with cargo molecules, and ...
... between a p24 protein and a cargo molecule has been reported. However, it is possible that p24-cargo interactions are weak and/or transient, and cannot be revealed by classical biochemical approaches. Alternatively, it is possible that p24 proteins do not directly interact with cargo molecules, and ...
Mucosal IL-10 and TGF-β play crucial roles in preventing LPS
... we believe to be novel. We identified the mucosal epithelial lining as an important source of IL-10. We also showed that mucosal IL-10 depletion by neutralizing anti–IL-10 antibodies induced a strong IFN-γ response that was responsible for the intestinal barrier disruption. Finally, we identified LP ...
... we believe to be novel. We identified the mucosal epithelial lining as an important source of IL-10. We also showed that mucosal IL-10 depletion by neutralizing anti–IL-10 antibodies induced a strong IFN-γ response that was responsible for the intestinal barrier disruption. Finally, we identified LP ...
High dose intravenous immunoglobulin treatment: Mechanisms of
... immune system is not as widely accepted, although the efficacy of IVIg treatment has been demonstrated in several autoimmune diseases.1-4 IVIg preparations are fractionated from a plasma pool of healthy donors and contain both immune antibodies and physiologic autoantibodies. As immune antibodies refl ...
... immune system is not as widely accepted, although the efficacy of IVIg treatment has been demonstrated in several autoimmune diseases.1-4 IVIg preparations are fractionated from a plasma pool of healthy donors and contain both immune antibodies and physiologic autoantibodies. As immune antibodies refl ...
Effects of Episodic Turbulence on Diatoms, with Comments on the Use of Evans Blue Stain for Live-Dead Determinations
... samples. * indicates significant difference before and after turbulence treatment (paired ttest; p<0.05)…..…………………………………………….…...……………………51-52 Figure A1: T. weissflogii cells treated with Evans Blue…………………………..….....53 Figure A2 (a): Experiment aquarium with propeller and ADV in place. The ADV and p ...
... samples. * indicates significant difference before and after turbulence treatment (paired ttest; p<0.05)…..…………………………………………….…...……………………51-52 Figure A1: T. weissflogii cells treated with Evans Blue…………………………..….....53 Figure A2 (a): Experiment aquarium with propeller and ADV in place. The ADV and p ...
IOSR Journal of Dental and Medical Sciences (IOSR-JDMS)
... Abstract: Recent studies suggest that regulatory T cells (Treg cells) and immunosuppressive cytokines such as interleukine-10 (IL-10) have a role in pathogenesis of asthma and efficacy of allergen specific immunotherapy (SIT). Since immunotherapy had limitations, several adjuvants proposed to improv ...
... Abstract: Recent studies suggest that regulatory T cells (Treg cells) and immunosuppressive cytokines such as interleukine-10 (IL-10) have a role in pathogenesis of asthma and efficacy of allergen specific immunotherapy (SIT). Since immunotherapy had limitations, several adjuvants proposed to improv ...
ABCB1 haplotypes do not influence transport Linköping University Post Print
... activity, or markers thereof, might be useful predictors of response to imatinib, and perhaps to the second-generation TKIs as well. The ABCB1 gene is highly polymorphic, with about 100 identified single-nucleotide polymorphisms (SNPs) located in the coding regions, some of which have been associate ...
... activity, or markers thereof, might be useful predictors of response to imatinib, and perhaps to the second-generation TKIs as well. The ABCB1 gene is highly polymorphic, with about 100 identified single-nucleotide polymorphisms (SNPs) located in the coding regions, some of which have been associate ...
Ceman, S, O Donnell, WT, Reed, M, Patton, S, Pohl, J and Warren, ST: Phosphorylation regulates translation state of FMRP-associated polyribosomes. Human Molecular Genetics 12:3295-3305 (2003).
... we used site-directed mutagenesis to replace this serine with either an alanine (Ala), which cannot be phosphorylated or an aspartic acid (Asp), which mimics phosphorylation (32). These stable transfectants do not over-express FMRP with each line expressing nearly equivalent amounts of modified FMRP ...
... we used site-directed mutagenesis to replace this serine with either an alanine (Ala), which cannot be phosphorylated or an aspartic acid (Asp), which mimics phosphorylation (32). These stable transfectants do not over-express FMRP with each line expressing nearly equivalent amounts of modified FMRP ...
PLEKHM1/DEF8/RAB7 complex regulates lysosome positioning and
... Osteoclasts are multinucleated cells that are capable of resorbing calcified cartilage and bone matrix during skeletal development, homeostasis, and repair (1). Increased osteoclast number or activity leads to bone loss in metabolic bone diseases, such as postmenopausal osteoporosis (2). Conversely, ...
... Osteoclasts are multinucleated cells that are capable of resorbing calcified cartilage and bone matrix during skeletal development, homeostasis, and repair (1). Increased osteoclast number or activity leads to bone loss in metabolic bone diseases, such as postmenopausal osteoporosis (2). Conversely, ...
Overcoming of Vincristine Resistance in P388
... rosea L., are commonly used as chemotherapeutic agents in the treatment of cancer (5, 25). Although the mechanism of action of the drugs has not been clearly elucidated, the major antitumor effect of these agents appears to be related to their action on tubulin and microtubules (19, 25). Microtubule ...
... rosea L., are commonly used as chemotherapeutic agents in the treatment of cancer (5, 25). Although the mechanism of action of the drugs has not been clearly elucidated, the major antitumor effect of these agents appears to be related to their action on tubulin and microtubules (19, 25). Microtubule ...
A Review of Centriole Activity, and Wrongful Activity, during Cell
... discusses the nucleation of these microtubules. The paper concludes with a description of centriole malfunction and overduplication (supernumerary centrioles), leading to clusters of centrioles —a hallmark of cancer cells. These centriole clusters thus form “biomarkers” for tumor imaging and treatme ...
... discusses the nucleation of these microtubules. The paper concludes with a description of centriole malfunction and overduplication (supernumerary centrioles), leading to clusters of centrioles —a hallmark of cancer cells. These centriole clusters thus form “biomarkers” for tumor imaging and treatme ...
Cornell University, 1989 M.S. Environmental Engineering Stanford University, 1990
... periods, low-level determinations of these nutrients were made to compliment the analyses of community structure. Size structure varied systematically, although not necessarily as a function of nutrient availability. Two parameters were explored: 1) spectral slope, which indicates the relative contr ...
... periods, low-level determinations of these nutrients were made to compliment the analyses of community structure. Size structure varied systematically, although not necessarily as a function of nutrient availability. Two parameters were explored: 1) spectral slope, which indicates the relative contr ...
PDF
... its cytoplasmic relocalization within 6 h (Warmflash et al., 2012). Thus, we searched for genes that could either prevent GFP-Smad4 nuclear localization at 1 h or prevent its return to the cytoplasm by 6 h. To perform the screen, C2C12 GFP-Smad4 reporter cells were transfected with pools of three sm ...
... its cytoplasmic relocalization within 6 h (Warmflash et al., 2012). Thus, we searched for genes that could either prevent GFP-Smad4 nuclear localization at 1 h or prevent its return to the cytoplasm by 6 h. To perform the screen, C2C12 GFP-Smad4 reporter cells were transfected with pools of three sm ...
Four-cell stage mouse blastomeres have different developmental
... two- to four-cell stage division. Embryos were examined at the fourcell stage and those with tetrahedral morphology (three blastomeres gathered around the attached polar body and the fourth one more distal) were scored from the position of labelled and unlabeled blastomeres as being either ME or EM ...
... two- to four-cell stage division. Embryos were examined at the fourcell stage and those with tetrahedral morphology (three blastomeres gathered around the attached polar body and the fourth one more distal) were scored from the position of labelled and unlabeled blastomeres as being either ME or EM ...
Evolutionarily conserved recognition and innate immunity to fungal
... of peritoneal macrophages with anti-SCARF1 antibodies confirmed colocalization of the receptor with areas of C. neoformans attachment (Fig. 3 a, bottom). To further investigate the role of SCARF1 in the recognition of C. neoformans, we examined the ability of several soluble fungal-derived carbohydr ...
... of peritoneal macrophages with anti-SCARF1 antibodies confirmed colocalization of the receptor with areas of C. neoformans attachment (Fig. 3 a, bottom). To further investigate the role of SCARF1 in the recognition of C. neoformans, we examined the ability of several soluble fungal-derived carbohydr ...
- the University of Huddersfield Repository
... glutathione peroxidase; this has been well demonstrated in models of mouse-based cutaneous injury [31]. In fact, the overall evidence for H2O2 having an integral role in stress/inflammatory responses and the subsequent tissue/neuron repair process is striking. The functional role of H2O2 is due to s ...
... glutathione peroxidase; this has been well demonstrated in models of mouse-based cutaneous injury [31]. In fact, the overall evidence for H2O2 having an integral role in stress/inflammatory responses and the subsequent tissue/neuron repair process is striking. The functional role of H2O2 is due to s ...
Peptides that mimic Candida albicans–derived β
... also a common fungal pathogen that can cause life-threatening infections in immunocompromised individuals (Odds, 1994). Chemical studies of C. albicans PPM have led to the identification of unusually linked mannose residues that are absent from S. cerevisiae PPM. These β-1,2-linked oligomannosides a ...
... also a common fungal pathogen that can cause life-threatening infections in immunocompromised individuals (Odds, 1994). Chemical studies of C. albicans PPM have led to the identification of unusually linked mannose residues that are absent from S. cerevisiae PPM. These β-1,2-linked oligomannosides a ...
CELL WALLS OF GROWING PLANT CELLS
... microfibrils together by forming molecular tethers, which is referred to as the ‘sticky network’ model. Plant growth occurs when these tethers are peeled from the microfibrils by expansins or broken by glycosidases or transglycosylases. A number of researchers have presented theoretical difficulties ...
... microfibrils together by forming molecular tethers, which is referred to as the ‘sticky network’ model. Plant growth occurs when these tethers are peeled from the microfibrils by expansins or broken by glycosidases or transglycosylases. A number of researchers have presented theoretical difficulties ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.