Role of the spindle pole body of yeast in mediating assembly of the
... A hallmark of all previously identi®ed proteins of the SPB core particle (with the exception of Nud1p) is the presence of coiled-coil motifs (Wigge et al., 1998). To identify proteins that are speci®c for SPBs of meiotic cells, we analyzed genes that are transcriptionally upregulated during meiosis ...
... A hallmark of all previously identi®ed proteins of the SPB core particle (with the exception of Nud1p) is the presence of coiled-coil motifs (Wigge et al., 1998). To identify proteins that are speci®c for SPBs of meiotic cells, we analyzed genes that are transcriptionally upregulated during meiosis ...
Thesis - KI Open Archive
... recognizes conserved microbial patterns and cannot differentiate between small variations in foreign particles and it does not have the capacity to memorize a pathogen exposure. It is activated within hours of infection and comprises phagocytes, such as macrophages, dendritic cells (DCs), and polymo ...
... recognizes conserved microbial patterns and cannot differentiate between small variations in foreign particles and it does not have the capacity to memorize a pathogen exposure. It is activated within hours of infection and comprises phagocytes, such as macrophages, dendritic cells (DCs), and polymo ...
Cellular lipidomics
... inside of the membrane (Lisanti et al, 1994; Parton, 1994) or activated receptors, a lipid, like ceramide generated by signaling sphingomyelinases (Gulbins et al, 2004; London and London, 2004), or lipid-anchored proteins (Brügger et al, 2004). In lipid transport, the forming lipid domain must be s ...
... inside of the membrane (Lisanti et al, 1994; Parton, 1994) or activated receptors, a lipid, like ceramide generated by signaling sphingomyelinases (Gulbins et al, 2004; London and London, 2004), or lipid-anchored proteins (Brügger et al, 2004). In lipid transport, the forming lipid domain must be s ...
central spindle and contractile ring for cytokinesis encodes a kinesin
... proteins, dissociate from chromosomes at the metaphase–anaphase transition to be deposited at the cell equator. The inner centromere proteins (INCENPs), for example, transfer to the central spindle and the cell cortex and are necessary for completion of cytokinesis (Eckley et al. 1997; Earnshaw and ...
... proteins, dissociate from chromosomes at the metaphase–anaphase transition to be deposited at the cell equator. The inner centromere proteins (INCENPs), for example, transfer to the central spindle and the cell cortex and are necessary for completion of cytokinesis (Eckley et al. 1997; Earnshaw and ...
siRNA back - Santa Cruz Biotechnology
... (Fluorescein Conjugates): sc-36869, sc-44239, sc-44240 or sc-44241. Each contain a scrambled sequence that will not lead to the specific degradation of any known cellular mRNA. ...
... (Fluorescein Conjugates): sc-36869, sc-44239, sc-44240 or sc-44241. Each contain a scrambled sequence that will not lead to the specific degradation of any known cellular mRNA. ...
Standard PDF - Wiley Online Library
... photon energy. The inhomogeneity inherent to most biological specimen, and in particular, to the internal structure of various cells, leads to high quality SHG images without any preconditioning such as labelling or staining that might induce undesirable effects in the living cell (Reshak, 2009). Hi ...
... photon energy. The inhomogeneity inherent to most biological specimen, and in particular, to the internal structure of various cells, leads to high quality SHG images without any preconditioning such as labelling or staining that might induce undesirable effects in the living cell (Reshak, 2009). Hi ...
CHAPTER 3: Early tissue patterning is recreated by MEFs in... Figure 5: Osteogenic and chondrogenic differentiation of MEFs cultured in
... Moreover, a dose-response inhibitory effect on the 3D-bilateral structure development can be observed when diluted concentrations of Staurosporine are added to the cultures (Figure 2 F). These results indicate that during this morphogenetic event the cell density increases not only by contraction bu ...
... Moreover, a dose-response inhibitory effect on the 3D-bilateral structure development can be observed when diluted concentrations of Staurosporine are added to the cultures (Figure 2 F). These results indicate that during this morphogenetic event the cell density increases not only by contraction bu ...
cells
... – netlike array of protein filaments (intermediate filaments; lamin polymers) embedded in inner membrane – maintains nuclear shape – mutations in lamins & lamin-binding proteins cause a wide range of human diseases, collectively termed laminopathies including muscular dystrophy & accelerated aging C ...
... – netlike array of protein filaments (intermediate filaments; lamin polymers) embedded in inner membrane – maintains nuclear shape – mutations in lamins & lamin-binding proteins cause a wide range of human diseases, collectively termed laminopathies including muscular dystrophy & accelerated aging C ...
pET System Manual
... Almost all of the pET vectors can express proteins that do not contain vector-encoded sequences. An Nde I or Nco I site is available in many vectors for cloning into the AUG start codon at the 5' end of the insert coding sequence. Similarly, proteins without vector-encoded C-terminal fusions are obt ...
... Almost all of the pET vectors can express proteins that do not contain vector-encoded sequences. An Nde I or Nco I site is available in many vectors for cloning into the AUG start codon at the 5' end of the insert coding sequence. Similarly, proteins without vector-encoded C-terminal fusions are obt ...
Seasonal Changes of Plasma Membrane H
... The plasma membrane H⫹-ATPase (PM H⫹-ATPase), potassium ions, and endogenous ion currents might play a fundamental role in the physiology of cambial growth. Seasonal changes of these parameters were studied in twigs of Populus nigra and Populus trichocarpa. Monoclonal and polyclonal antibodies again ...
... The plasma membrane H⫹-ATPase (PM H⫹-ATPase), potassium ions, and endogenous ion currents might play a fundamental role in the physiology of cambial growth. Seasonal changes of these parameters were studied in twigs of Populus nigra and Populus trichocarpa. Monoclonal and polyclonal antibodies again ...
The Neurosecretory Neuron in Neuroendocrine
... 1963; Nalbandov, 1963; Bajusz and Jasmin, 1964; E. Scharrer, 1966; Weitzman, 19641966). Only some of these will be included in the following discussion whose central topic is the elucidation of the phenomenon of neurosecretion and its role in neuroendocrine integration. Even at that, current researc ...
... 1963; Nalbandov, 1963; Bajusz and Jasmin, 1964; E. Scharrer, 1966; Weitzman, 19641966). Only some of these will be included in the following discussion whose central topic is the elucidation of the phenomenon of neurosecretion and its role in neuroendocrine integration. Even at that, current researc ...
RCAI Annual Report 2006 (PDF 5.6M)
... rather than competing with the universities, can complement the university research system. What is most significant with RCAI is the focus on young investigators at the beginning of their career. Well over two-thirds of the research laboratories at the Center are lead by young investigators, many o ...
... rather than competing with the universities, can complement the university research system. What is most significant with RCAI is the focus on young investigators at the beginning of their career. Well over two-thirds of the research laboratories at the Center are lead by young investigators, many o ...
Electrochemical detection of biological reactions using a novel nano
... on a silicon chip. Each of the electrochemical cells can be monitored simultaneously and independently, and each cell contains three embedded electrodes, which enable performance of all types of electrochemical measurements. The integration of living organisms on an electrochemical array chip that c ...
... on a silicon chip. Each of the electrochemical cells can be monitored simultaneously and independently, and each cell contains three embedded electrodes, which enable performance of all types of electrochemical measurements. The integration of living organisms on an electrochemical array chip that c ...
Myosin-Powered Membrane Compartment Drives Cytoplasmic
... are much less understood. The motor proteins driving streaming are known for Drosophila oocytes [6,14], Caenorhabditis elegans embryos [15] and rapidly moving fish keratocytes [16], whereas the motor cargoes that entrain cytosol are yet to be identified. It has also been proposed that the Arp2/3-dep ...
... are much less understood. The motor proteins driving streaming are known for Drosophila oocytes [6,14], Caenorhabditis elegans embryos [15] and rapidly moving fish keratocytes [16], whereas the motor cargoes that entrain cytosol are yet to be identified. It has also been proposed that the Arp2/3-dep ...
gland - Sinoe Medical Association
... Major Types of Glands: The two types are based on the mechanism of their secretion. Exocrine Glands – Glands that secrete their products onto the apical (or epithelia) surface directly OR via epithelial ducts or tubes that are connected to the apical surface. These exocrine glands are composed of h ...
... Major Types of Glands: The two types are based on the mechanism of their secretion. Exocrine Glands – Glands that secrete their products onto the apical (or epithelia) surface directly OR via epithelial ducts or tubes that are connected to the apical surface. These exocrine glands are composed of h ...
... the membranes of other mature epithelial cells from the respiratory mucosa, such as the secretory cells, or in the mucus they produce [58]. The lack of the sialo longchain receptor structure in the secreted mucus seems to be an important factor favouring bacterial adhesion to ciliated cells, rather ...
Tendon development and musculoskeletal assembly: emerging
... connectivity lies, within the TPCs or within the muscles themselves? Chick-quail chimera studies suggests that, at least for cranial and limb muscles, the TPCs and tendon matrix determine the pattern of attachments (Kardon, 1998; Kieny and Chevallier, 1979; Noden, 1988). Do muscles and the myomatrix ...
... connectivity lies, within the TPCs or within the muscles themselves? Chick-quail chimera studies suggests that, at least for cranial and limb muscles, the TPCs and tendon matrix determine the pattern of attachments (Kardon, 1998; Kieny and Chevallier, 1979; Noden, 1988). Do muscles and the myomatrix ...
Dynamics of the slowing segmentation clock reveal
... her1 5′- and 3′-UTRs to facilitate rapid transcript turnover (Delaune et al., 2012). Using this reporter, we described the behavior of oscillating cells at a local level, confirming that the Notch pathway synchronizes neighboring cells and revealing that daughter cells oscillate synchronously after ...
... her1 5′- and 3′-UTRs to facilitate rapid transcript turnover (Delaune et al., 2012). Using this reporter, we described the behavior of oscillating cells at a local level, confirming that the Notch pathway synchronizes neighboring cells and revealing that daughter cells oscillate synchronously after ...
GLABRA2 Directly Suppresses Basic Helix-Loop
... VP16-GL2ΔN gene detected 864 genes the steady-state transcript levels of which were consistently increased by more than two fold after dexamethasone (DEX) treatment (Supplemental Table 1). These candidate genes were categorized by gene product functions as defined using the agriGO web-based software ...
... VP16-GL2ΔN gene detected 864 genes the steady-state transcript levels of which were consistently increased by more than two fold after dexamethasone (DEX) treatment (Supplemental Table 1). These candidate genes were categorized by gene product functions as defined using the agriGO web-based software ...
The skull is more than head and face
... - The Skull is more than Head and Face 1. The Complexity of Growth Patterns Unfolded 2. The Pattern of Ascensus (Cerebralization) 3. The Pattern of Descensus ...
... - The Skull is more than Head and Face 1. The Complexity of Growth Patterns Unfolded 2. The Pattern of Ascensus (Cerebralization) 3. The Pattern of Descensus ...
HIV-1 Evolves into a Nonsyncytium-Inducing Virus upon Prolonged
... loss of infectivity on the other cell lines. Although speculative, these differences may be relevant for discerning the mechanism(s) behind each phenotype. Env mutations do not change the coreceptor usage The T-cell tropic LAI virus uses the CXCR4 protein as coreceptor for infection. Because the Env ...
... loss of infectivity on the other cell lines. Although speculative, these differences may be relevant for discerning the mechanism(s) behind each phenotype. Env mutations do not change the coreceptor usage The T-cell tropic LAI virus uses the CXCR4 protein as coreceptor for infection. Because the Env ...
DISCUSSION The actin cytoskeleton mediates a variety of essential processes in... including cell motility, cell shape, phagocytosis, and cytokinesis. Three distinct...
... DISCUSSION The actin cytoskeleton mediates a variety of essential processes in all eukaryotic cells, including cell motility, cell shape, phagocytosis, and cytokinesis. Three distinct kinds of actin-based structures have been identified, which are regulated by the Rho family of GTPases: Cdc42 induce ...
... DISCUSSION The actin cytoskeleton mediates a variety of essential processes in all eukaryotic cells, including cell motility, cell shape, phagocytosis, and cytokinesis. Three distinct kinds of actin-based structures have been identified, which are regulated by the Rho family of GTPases: Cdc42 induce ...
5 | structure and function of plasma membranes
... interaction with its environment (see Table 5.1 for a summary). Cells exclude some substances, take in others, and excrete still others, all in controlled quantities. The plasma membrane must be very flexible to allow certain cells, such as red blood cells and white blood cells, to change shape as t ...
... interaction with its environment (see Table 5.1 for a summary). Cells exclude some substances, take in others, and excrete still others, all in controlled quantities. The plasma membrane must be very flexible to allow certain cells, such as red blood cells and white blood cells, to change shape as t ...
Identification of Bacterial Species
... The laboratory exercise is designed to introduce first-year cell biology students to several standard microbiological tests that can be used in an initial identification of an unknown bacterial species. In the first week, students are provided with a mixed bacterial culture containing three species, ...
... The laboratory exercise is designed to introduce first-year cell biology students to several standard microbiological tests that can be used in an initial identification of an unknown bacterial species. In the first week, students are provided with a mixed bacterial culture containing three species, ...
Cellular differentiation
In developmental biology, cellular differentiation isa cell changes from one cell type to another. Most commonly this is a less specialized type becoming a more specialized type, such as during cell growth. Differentiation occurs numerous times during the development of a multicellular organism as it changes from a simple zygote to a complex system of tissues and cell types. Differentiation continues in adulthood as adult stem cells divide and create fully differentiated daughter cells during tissue repair and during normal cell turnover. Some differentiation occurs in response to antigen exposure. Differentiation dramatically changes a cell's size, shape, membrane potential, metabolic activity, and responsiveness to signals. These changes are largely due to highly controlled modifications in gene expression and are the study of epigenetics. With a few exceptions, cellular differentiation almost never involves a change in the DNA sequence itself. Thus, different cells can have very different physical characteristics despite having the same genome.A cell that can differentiate into all cell types of the adult organism is known as pluripotent. Such cells are called embryonic stem cells in animals and meristematic cells in higher plants. A cell that can differentiate into all cell types, including the placental tissue, is known as totipotent. In mammals, only the zygote and subsequent blastomeres are totipotent, while in plants many differentiated cells can become totipotent with simple laboratory techniques. In cytopathology, the level of cellular differentiation is used as a measure of cancer progression. ""Grade"" is a marker of how differentiated a cell in a tumor is.