Bradykinin stimulates IL-6 and IL-8 production by human lung
... isolated guinea pig lung strips [12], and IL-1 from cultured human lung fibroblasts [13]. These data suggest that BK may be involved in the control of the inflammatory reaction associated with interstitial lung disorders through proinflammatory cytokines production by lung fibroblasts. It has also b ...
... isolated guinea pig lung strips [12], and IL-1 from cultured human lung fibroblasts [13]. These data suggest that BK may be involved in the control of the inflammatory reaction associated with interstitial lung disorders through proinflammatory cytokines production by lung fibroblasts. It has also b ...
Drosophila embryos close epithelial wounds using a combination of
... that Drosophila embryos may be able to repair lesions using ...
... that Drosophila embryos may be able to repair lesions using ...
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... histological sections, we never observed Lgl2 localisation at the basal cortex of the basal epidermal cells, where hemidesmosomes form (Fig. 1H). Instead, Lgl2 was predominantly localised to the lateral domain in the basal epidermal cells (Fig. 1H). The apparent apical staining in the basal epiderma ...
... histological sections, we never observed Lgl2 localisation at the basal cortex of the basal epidermal cells, where hemidesmosomes form (Fig. 1H). Instead, Lgl2 was predominantly localised to the lateral domain in the basal epidermal cells (Fig. 1H). The apparent apical staining in the basal epiderma ...
Linköping University Post Print
... Over the last decade, the lysosome has emerged as a significant component of the cellular death machinery. Lysosomes, which were first described by de Duve and colleagues in 1955 [1], are acidic, single-membrane bound organelles that are present in all eukaryotic cells [2]. The primary function of l ...
... Over the last decade, the lysosome has emerged as a significant component of the cellular death machinery. Lysosomes, which were first described by de Duve and colleagues in 1955 [1], are acidic, single-membrane bound organelles that are present in all eukaryotic cells [2]. The primary function of l ...
In vitro skin models to study epithelial regeneration from the hair
... permanent cover but, availability can be restricted due to sparse donor sites which can lead to pain and scar formation. The latter are temporary, subject to rejection, and pose a risk of viral transmission. Utilisation of keratinocyte sheets in the treatment of burns has also been documented [2]. H ...
... permanent cover but, availability can be restricted due to sparse donor sites which can lead to pain and scar formation. The latter are temporary, subject to rejection, and pose a risk of viral transmission. Utilisation of keratinocyte sheets in the treatment of burns has also been documented [2]. H ...
In tobacco leaf epidermal cells, the integrity of protein export from
... The drug BFA, which blocks the activation of Arf1 (Robineau et al., 2000), prevents cargo-induced recruitment of a fluorescent fusion of Sar1 at the ERES in tobacco leaf epidermal cells (daSilva et al., 2004). In contrast, BFA does not have an effect on the distribution of ERES labelled by Sec13-GFP ...
... The drug BFA, which blocks the activation of Arf1 (Robineau et al., 2000), prevents cargo-induced recruitment of a fluorescent fusion of Sar1 at the ERES in tobacco leaf epidermal cells (daSilva et al., 2004). In contrast, BFA does not have an effect on the distribution of ERES labelled by Sec13-GFP ...
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... 393). Because all three channels can be activated by hyposmotic stress, this indicates that they are probably activated sequentially to provide a gradual efflux conduit (147, 168). Studies of the E. coli MscS channel structure demonstrated that it folds as heptamer with three transmembrane helices ( ...
... 393). Because all three channels can be activated by hyposmotic stress, this indicates that they are probably activated sequentially to provide a gradual efflux conduit (147, 168). Studies of the E. coli MscS channel structure demonstrated that it folds as heptamer with three transmembrane helices ( ...
In tobacco leaf epidermal cells, the integrity of protein export from
... The drug BFA, which blocks the activation of Arf1 (Robineau et al., 2000), prevents cargo-induced recruitment of a fluorescent fusion of Sar1 at the ERES in tobacco leaf epidermal cells (daSilva et al., 2004). In contrast, BFA does not have an effect on the distribution of ERES labelled by Sec13-GFP ...
... The drug BFA, which blocks the activation of Arf1 (Robineau et al., 2000), prevents cargo-induced recruitment of a fluorescent fusion of Sar1 at the ERES in tobacco leaf epidermal cells (daSilva et al., 2004). In contrast, BFA does not have an effect on the distribution of ERES labelled by Sec13-GFP ...
Pretreatment Increases Cytokine-induced NO Production
... iNOS antibody was obtained from Transduction Laboratories (Lexington, KY). SNAP and oxidized SNAP were prepared as described previously (19, 20). Red blood cells were prepared by collecting blood from rats as previously described (21). Antibodies for GTPCHI and GFRP were generated by Dr. Kazuyuki Ha ...
... iNOS antibody was obtained from Transduction Laboratories (Lexington, KY). SNAP and oxidized SNAP were prepared as described previously (19, 20). Red blood cells were prepared by collecting blood from rats as previously described (21). Antibodies for GTPCHI and GFRP were generated by Dr. Kazuyuki Ha ...
Fcg Receptor Deficiency Attenuates Diabetic Nephropathy
... contribution of IgG Fcg receptors to diabetic renal injury in hyperglycemic, hypercholesterolemic mice. We used streptozotocin to induce diabetes in apolipoprotein E–deficient mice and in mice deficient in both apolipoprotein E and g-chain, the common subunit of activating Fcg receptors. After 15 week ...
... contribution of IgG Fcg receptors to diabetic renal injury in hyperglycemic, hypercholesterolemic mice. We used streptozotocin to induce diabetes in apolipoprotein E–deficient mice and in mice deficient in both apolipoprotein E and g-chain, the common subunit of activating Fcg receptors. After 15 week ...
Keystone Exam Study Guide
... BIO.A.2.1.1 Describe the unique properties of water and how these properties support life on Earth (e.g., freezing point, high specific heat, cohesion). ...
... BIO.A.2.1.1 Describe the unique properties of water and how these properties support life on Earth (e.g., freezing point, high specific heat, cohesion). ...
effects of light on photosynthesis, grazing, and population dynamics
... RhodPfiest1) and was then maintained for 48 h without feeding. The culture was divided into six 50-mL aliquots with P. piscicida concentrations of 7500 cellsmL1. Three of the six cultures were wrapped in aluminum foil, and R. sp. was added to the six experimental and to six R. sp. control flasks ...
... RhodPfiest1) and was then maintained for 48 h without feeding. The culture was divided into six 50-mL aliquots with P. piscicida concentrations of 7500 cellsmL1. Three of the six cultures were wrapped in aluminum foil, and R. sp. was added to the six experimental and to six R. sp. control flasks ...
Brassinosteroid Signal Transduction from Receptor Kinases to
... amino acid residues deleted, displayed higher kinase activity in vitro and more complete rescue of the bri1 mutant phenotype in transgenic plants, suggesting an autoinhibitory role of the CT (92). The CT region contains multiple phosphorylation sites, and mutation of these Ser/Thr residues to phosph ...
... amino acid residues deleted, displayed higher kinase activity in vitro and more complete rescue of the bri1 mutant phenotype in transgenic plants, suggesting an autoinhibitory role of the CT (92). The CT region contains multiple phosphorylation sites, and mutation of these Ser/Thr residues to phosph ...
Vitronectin is expressed in the ventral region of the neural tube and
... from cultured retinal neurons (Neugebauer et al., 1991) and the appearance of neurons in cultured retinal neuroepithelial cells (Martínez-Morales et al., 1995), no role in the differentiation of specific neuronal phenotypes has been established. We have cloned chick vitronectin and studied its expre ...
... from cultured retinal neurons (Neugebauer et al., 1991) and the appearance of neurons in cultured retinal neuroepithelial cells (Martínez-Morales et al., 1995), no role in the differentiation of specific neuronal phenotypes has been established. We have cloned chick vitronectin and studied its expre ...
Chapter 17. Cytoskeleton Chapter 17. Cytoskeleton Chapter 17
... Chapter 17. Cytoskeleton • Cells are not “bags of enzymes”, but rather organized in three dimensions. • This organization is carried out by the cytoskeleton, the “bones and muscles” (and more) of cells. • Cells are thousands of times larger than a typical molecule. Therefore the cytoskeleton must lo ...
... Chapter 17. Cytoskeleton • Cells are not “bags of enzymes”, but rather organized in three dimensions. • This organization is carried out by the cytoskeleton, the “bones and muscles” (and more) of cells. • Cells are thousands of times larger than a typical molecule. Therefore the cytoskeleton must lo ...
Convergent evolution of neural systems in ctenophores
... conclusion – they suggested that Ctenophora is the earliest branching animal lineage, sister to all metazoans (Fig. 1). The verdict may not yet be considered final because of the limited amount of comparative data available and the complexity of statistical analysis in phylogenomic studies. For exam ...
... conclusion – they suggested that Ctenophora is the earliest branching animal lineage, sister to all metazoans (Fig. 1). The verdict may not yet be considered final because of the limited amount of comparative data available and the complexity of statistical analysis in phylogenomic studies. For exam ...
Pax1/Pax9 and vertebral column development
... as well as ossified fusions between vertebrae and neural arches are found (Fig. 1E,F). Thus, Pax1 and Pax9 interact during vertebral column development. This result is more clearly demonstrated in mutants that lack both copies of Pax1: in Pax1−/−; Pax9−/− double homozygous mutants, no vertebral bodi ...
... as well as ossified fusions between vertebrae and neural arches are found (Fig. 1E,F). Thus, Pax1 and Pax9 interact during vertebral column development. This result is more clearly demonstrated in mutants that lack both copies of Pax1: in Pax1−/−; Pax9−/− double homozygous mutants, no vertebral bodi ...
Anti-HIV-1 activity of Trim 37
... Since we were not able to fully knockdown endogenous Trim 37 gene expression in 293T cells, we further investigated the nature of the Trim 37 gene locus. The Trim 37 gene locus encodes two transcript variants, TV1 and TV2, which lead to the production of the same protein product. We used qPCR to det ...
... Since we were not able to fully knockdown endogenous Trim 37 gene expression in 293T cells, we further investigated the nature of the Trim 37 gene locus. The Trim 37 gene locus encodes two transcript variants, TV1 and TV2, which lead to the production of the same protein product. We used qPCR to det ...
Conserved mechanisms of Ras regulation of evolutionary
... Figure 3), whereas the individual activation domains RI and RIII alone had very low activity [less than twothreefold, compare G-RI and G-RIII with GaI4 (1 ± 147) and see (Schneikert et al., 1992)]. The regulatory domain RII was inactive on its own (G-RII), whereas combining it with RI or RIII led to ...
... Figure 3), whereas the individual activation domains RI and RIII alone had very low activity [less than twothreefold, compare G-RI and G-RIII with GaI4 (1 ± 147) and see (Schneikert et al., 1992)]. The regulatory domain RII was inactive on its own (G-RII), whereas combining it with RI or RIII led to ...
Symplasmic networks in secondary vascular tissues
... time (see Carlquist, 2001a for an excellent review), but far less so in phloem where parenchyma is difficult to distinguish from neighbouring sieve element and companion or Strasburger cells, and cellular organization is lost due to sieve element collapse and ray dilatation (see discussion on longev ...
... time (see Carlquist, 2001a for an excellent review), but far less so in phloem where parenchyma is difficult to distinguish from neighbouring sieve element and companion or Strasburger cells, and cellular organization is lost due to sieve element collapse and ray dilatation (see discussion on longev ...
Hepatitis A Virus Adaptation to Cellular Shutoff Is Driven by Dynamic
... specific condition of AMD, infected cells (multiplicity of infection [MOI] of 1) from a T-175 flask were harvested by trypsin treatment, collected by centrifugation, resuspended in 500 l of NT buffer (0.1 M NaCl, 10 mM Tris-HCl, 1% NP-40, pH 7.4), and incubated for 30 min at room temperature. These ...
... specific condition of AMD, infected cells (multiplicity of infection [MOI] of 1) from a T-175 flask were harvested by trypsin treatment, collected by centrifugation, resuspended in 500 l of NT buffer (0.1 M NaCl, 10 mM Tris-HCl, 1% NP-40, pH 7.4), and incubated for 30 min at room temperature. These ...
The association of sore throat and psoriasis might be explained by
... approximately 2% of people of both sexes [5], with diminished quality of life [6] and significant co-morbidities [7]. While psoriasis has been established to be a complex genetic disease [8], environmental factors such as trauma and stress can play a role in its elicitation [1]. Throat infections by ...
... approximately 2% of people of both sexes [5], with diminished quality of life [6] and significant co-morbidities [7]. While psoriasis has been established to be a complex genetic disease [8], environmental factors such as trauma and stress can play a role in its elicitation [1]. Throat infections by ...
ppt
... Our goal: To analyze the retention time behavior of DRAM cells in modern DRAM devices to aid the collection of accurate profile information Our experiments: We characterize 248 modern commodity DDR3 DRAM chips from 5 manufacturers using an FPGA based testing platform Two Key Issues: 1. Data Pattern ...
... Our goal: To analyze the retention time behavior of DRAM cells in modern DRAM devices to aid the collection of accurate profile information Our experiments: We characterize 248 modern commodity DDR3 DRAM chips from 5 manufacturers using an FPGA based testing platform Two Key Issues: 1. Data Pattern ...
hydrogen peroxide, nitric oxide and hydrogen sulfide as part of the
... Small reactive compounds such as reactive oxygen species (ROS) and reactive nitrogen species (RNS) have been studied as components of cell signaling pathways for nearly thirty years1. Such compounds include hydrogen peroxide, singlet oxygen (O21) and superoxide anions (all considered as ROS), and ni ...
... Small reactive compounds such as reactive oxygen species (ROS) and reactive nitrogen species (RNS) have been studied as components of cell signaling pathways for nearly thirty years1. Such compounds include hydrogen peroxide, singlet oxygen (O21) and superoxide anions (all considered as ROS), and ni ...
multiple novel factors regulate the initiation of dna
... Duplication of the genome during S phase of the mitotic cell cycle begins at thousands of sites along chromosomes termed origins of replication. Although many of the essential protein components catalyzing events at these sites are known and are conserved throughout eukaryotes, the likelihood or eff ...
... Duplication of the genome during S phase of the mitotic cell cycle begins at thousands of sites along chromosomes termed origins of replication. Although many of the essential protein components catalyzing events at these sites are known and are conserved throughout eukaryotes, the likelihood or eff ...
Cellular differentiation
In developmental biology, cellular differentiation isa cell changes from one cell type to another. Most commonly this is a less specialized type becoming a more specialized type, such as during cell growth. Differentiation occurs numerous times during the development of a multicellular organism as it changes from a simple zygote to a complex system of tissues and cell types. Differentiation continues in adulthood as adult stem cells divide and create fully differentiated daughter cells during tissue repair and during normal cell turnover. Some differentiation occurs in response to antigen exposure. Differentiation dramatically changes a cell's size, shape, membrane potential, metabolic activity, and responsiveness to signals. These changes are largely due to highly controlled modifications in gene expression and are the study of epigenetics. With a few exceptions, cellular differentiation almost never involves a change in the DNA sequence itself. Thus, different cells can have very different physical characteristics despite having the same genome.A cell that can differentiate into all cell types of the adult organism is known as pluripotent. Such cells are called embryonic stem cells in animals and meristematic cells in higher plants. A cell that can differentiate into all cell types, including the placental tissue, is known as totipotent. In mammals, only the zygote and subsequent blastomeres are totipotent, while in plants many differentiated cells can become totipotent with simple laboratory techniques. In cytopathology, the level of cellular differentiation is used as a measure of cancer progression. ""Grade"" is a marker of how differentiated a cell in a tumor is.