Construction of high-density bacterial colony arrays and
... transferred into a centrifuge tube containing 5 mL sterilized water. This formed the original print suspension of bacteria. The cell concentration in the E. coli solution was determined to be 3107 cells/mL by the standard plate count method (Benson, 1998). This solution was diluted to different con ...
... transferred into a centrifuge tube containing 5 mL sterilized water. This formed the original print suspension of bacteria. The cell concentration in the E. coli solution was determined to be 3107 cells/mL by the standard plate count method (Benson, 1998). This solution was diluted to different con ...
Asymmetric Cell Division as a Route to Reduction in Cell Length
... microtubules occurs at the point at which the flagellum emerges from the cell body (Hemphill et al. 1991). Individual microtubules within the subpellicular array are stable with little turnover and the cytoskeleton is remarkably constant throughout the cell division cycle (Sherwin and Gull 1989a, 19 ...
... microtubules occurs at the point at which the flagellum emerges from the cell body (Hemphill et al. 1991). Individual microtubules within the subpellicular array are stable with little turnover and the cytoskeleton is remarkably constant throughout the cell division cycle (Sherwin and Gull 1989a, 19 ...
Rat embryonic ectoderm as renal isograft - Development
... the primitive streak is always attained when the early mouse embryo (or the embryonic body) is allowed to develop in vitro without disrupting its extraembryonic cavities and membranes (Wiley & Pedersen, 1977; Wiley, Spindle & Pedersen, 1978; Libbus & Hsu, 1980; Uno, 1982). The transformation of epit ...
... the primitive streak is always attained when the early mouse embryo (or the embryonic body) is allowed to develop in vitro without disrupting its extraembryonic cavities and membranes (Wiley & Pedersen, 1977; Wiley, Spindle & Pedersen, 1978; Libbus & Hsu, 1980; Uno, 1982). The transformation of epit ...
Isolation of Spherosomes with Lysosome Characteristics from
... nents from the soluble material indicates the pos sibility of an interaction between hydrolases and soluble factors. In fact the high speed supernatant contains an inhibitory factor for acid protease and phosphatase as demonstrated by determ inations of sedimentable activities in the presence or ab ...
... nents from the soluble material indicates the pos sibility of an interaction between hydrolases and soluble factors. In fact the high speed supernatant contains an inhibitory factor for acid protease and phosphatase as demonstrated by determ inations of sedimentable activities in the presence or ab ...
Histone modification and replacement in chromatin activation
... deletion of Suv39h1 and Suv39h2 genes (the double mutant will be referred to as Suv39h-null) reduced net methylation of histone H3 lysine 9 (H3-K9) by approximately one-half, and in wild-type cells Suv39h1 was seen to colocalize with the Polycomb group protein HP1 at heterochromatic regions of inter ...
... deletion of Suv39h1 and Suv39h2 genes (the double mutant will be referred to as Suv39h-null) reduced net methylation of histone H3 lysine 9 (H3-K9) by approximately one-half, and in wild-type cells Suv39h1 was seen to colocalize with the Polycomb group protein HP1 at heterochromatic regions of inter ...
THE SEPARATION OF DIFFERENT CELL CLASSES FROM
... ensure that the cells were fully active immediately on contact with the glass beads) with intermittent stirring with a Vortex mixer (Scientific Industries, Inc., Queens Village, N .Y.) (to minimize aggregation and adherence to the tube) . The suspension was then run onto the bead column, followed by ...
... ensure that the cells were fully active immediately on contact with the glass beads) with intermittent stirring with a Vortex mixer (Scientific Industries, Inc., Queens Village, N .Y.) (to minimize aggregation and adherence to the tube) . The suspension was then run onto the bead column, followed by ...
Tet System Approved FBS
... The problem: Trace tetracycline contaminants in standard Fetal Bovine Serum (FBS) can alter experimental results by enabling background expression in Tet-On® Systems and suppressing maximum expression levels in Tet-Off® systems. The solution: Tet System Approved FBS from Clontech, the only functiona ...
... The problem: Trace tetracycline contaminants in standard Fetal Bovine Serum (FBS) can alter experimental results by enabling background expression in Tet-On® Systems and suppressing maximum expression levels in Tet-Off® systems. The solution: Tet System Approved FBS from Clontech, the only functiona ...
Carr_Flagellum.pps
... A flagellum is on the end of every sperm cell Without a flagellum sperm would not be able to fertilize an egg ...
... A flagellum is on the end of every sperm cell Without a flagellum sperm would not be able to fertilize an egg ...
Histochemical Polymorphism of Keratin Pearls in Squamous Cell
... In compliance with the objectives of the study a complex of histological and histochemical methods has been applied during the investigation. The analysis has been carried out on post operational material, taken from 25 patients with keratinizing squamous cell carcinoma of lung. The obtained materia ...
... In compliance with the objectives of the study a complex of histological and histochemical methods has been applied during the investigation. The analysis has been carried out on post operational material, taken from 25 patients with keratinizing squamous cell carcinoma of lung. The obtained materia ...
Derivation of naïve human embryonic stem cells
... as a stable in vitro state. There are practical advantages that come with a human naïve state. Among them is ease of trypsin passage and developmental capacity. Whole animals can be generated from good naïve mESCs through tetraploid complementation (4), and mEpiSCs cannot contribute to chimerism. Be ...
... as a stable in vitro state. There are practical advantages that come with a human naïve state. Among them is ease of trypsin passage and developmental capacity. Whole animals can be generated from good naïve mESCs through tetraploid complementation (4), and mEpiSCs cannot contribute to chimerism. Be ...
Challenges in Endometrial biopsy
... arrangement or affiliation with one or more organizations that could be perceived as a direct or indirect conflict of interest in the content of this presentation. ...
... arrangement or affiliation with one or more organizations that could be perceived as a direct or indirect conflict of interest in the content of this presentation. ...
Characterization of a Negative Thyroid Hormone Response Element
... iological and therapeutic significance. l-T3 is the major active form of thyroid hormone and stimulates Na,K-ATPase activity and isoform messenger RNA (mRNA) content in a variety of mammalian tissues (7–9). Both transcriptional and posttranscriptional mechanisms have been implicated in the regulatio ...
... iological and therapeutic significance. l-T3 is the major active form of thyroid hormone and stimulates Na,K-ATPase activity and isoform messenger RNA (mRNA) content in a variety of mammalian tissues (7–9). Both transcriptional and posttranscriptional mechanisms have been implicated in the regulatio ...
Protoplast Culture: definition
... Once the protoplasts have regenerated a cell wall, they undergo cell division and form a callus.This callus can be subcultured. The callus may undergo embryogenesis or organogenesis after about 3-4 weeks, in the correct culture conditions. The ...
... Once the protoplasts have regenerated a cell wall, they undergo cell division and form a callus.This callus can be subcultured. The callus may undergo embryogenesis or organogenesis after about 3-4 weeks, in the correct culture conditions. The ...
Beginnings of a Good Apoptotic Meal: The Find-Me and
... that S1P kinase 2 (SphK2) itself is a target of caspase 1 and that the cleaved fragment of SphK2 could be ‘‘released’’ from dying cells into the extracellular space where it would generate S1P (Weigert et al., 2010). Given the well-known role of S1P as a migratory signal and the pharmacological inte ...
... that S1P kinase 2 (SphK2) itself is a target of caspase 1 and that the cleaved fragment of SphK2 could be ‘‘released’’ from dying cells into the extracellular space where it would generate S1P (Weigert et al., 2010). Given the well-known role of S1P as a migratory signal and the pharmacological inte ...
Nucleotide sequences and further characterization of human
... cell lines represent once-circulating or still-circulating genomes ; (2) potentially pathogenic changes in HPV genomic physical state or phenotype identified in these cell lines are not an artifact of medical intervention or cell culture. Before assessing whether the particular sequences or physical ...
... cell lines represent once-circulating or still-circulating genomes ; (2) potentially pathogenic changes in HPV genomic physical state or phenotype identified in these cell lines are not an artifact of medical intervention or cell culture. Before assessing whether the particular sequences or physical ...
Nuclear centering in Spirogyra
... scaold as well as in the connected peripheral cytoplasm (pc). B Focus set slightly above the chloroplast bands (alignment of plastid long axes indicated by arrowheads) in the peripheral cytoplasm, with subcortical bundles of F-actin and short bundles of micro®laments (small arrows) normal to the ch ...
... scaold as well as in the connected peripheral cytoplasm (pc). B Focus set slightly above the chloroplast bands (alignment of plastid long axes indicated by arrowheads) in the peripheral cytoplasm, with subcortical bundles of F-actin and short bundles of micro®laments (small arrows) normal to the ch ...
![[Frontiers in Bioscience, 5, d50-57, January 1, 2000] THE CONTROL](http://s1.studyres.com/store/data/017171575_1-70635603f98612e3c5ac310e8691f919-300x300.png)
[Frontiers in Bioscience, 5, d50-57, January 1, 2000] THE CONTROL
... to localize to kinetochores that are not yet attached to the mitotic spindle (78,90,97,107-109). Similar to its human counterpart, the murine homolog of yeast Bub1p localizes to unattached kinetochores during mitosis (99). Additional genetic and biochemical studies have also shown that Bub1p, Bub3p, ...
... to localize to kinetochores that are not yet attached to the mitotic spindle (78,90,97,107-109). Similar to its human counterpart, the murine homolog of yeast Bub1p localizes to unattached kinetochores during mitosis (99). Additional genetic and biochemical studies have also shown that Bub1p, Bub3p, ...
Associative learning increases adult neurogenesis during a critical
... Fig. 2. Effects of trace eyeblink conditioning on survival of newly generated neurons. (A) Diagram illustrating the stage of development when BrdU-labeled cells were exposed to training. All animals were injected once with BrdU. They were then trained or not 30 min, 1 week or 3 weeks later. To asses ...
... Fig. 2. Effects of trace eyeblink conditioning on survival of newly generated neurons. (A) Diagram illustrating the stage of development when BrdU-labeled cells were exposed to training. All animals were injected once with BrdU. They were then trained or not 30 min, 1 week or 3 weeks later. To asses ...
Chapter 3 PDF
... The variety of cell types found in living things is staggering. Your body alone is made of trillions of cells of many different shapes, sizes, and functions. They include long, thin nerve cells that transmit sensory information, as well as short, blocky skin cells that cover and protect the body. De ...
... The variety of cell types found in living things is staggering. Your body alone is made of trillions of cells of many different shapes, sizes, and functions. They include long, thin nerve cells that transmit sensory information, as well as short, blocky skin cells that cover and protect the body. De ...
Chapter 3 PDF
... The variety of cell types found in living things is staggering. Your body alone is made of trillions of cells of many different shapes, sizes, and functions. They include long, thin nerve cells that transmit sensory information, as well as short, blocky skin cells that cover and protect the body. De ...
... The variety of cell types found in living things is staggering. Your body alone is made of trillions of cells of many different shapes, sizes, and functions. They include long, thin nerve cells that transmit sensory information, as well as short, blocky skin cells that cover and protect the body. De ...
BD Pharmingen™ Transcription Factor Buffer Set
... time.” It is the developer’s experience that this does not affect the protocol and might even improve S/N by allowing diffusion of unbound antibody. This step is convenient for multitasking. Rather than doing the 2X perm/wash step at the end of the protocol, an alternative is to allow stained cells ...
... time.” It is the developer’s experience that this does not affect the protocol and might even improve S/N by allowing diffusion of unbound antibody. This step is convenient for multitasking. Rather than doing the 2X perm/wash step at the end of the protocol, an alternative is to allow stained cells ...
BASIC TECHNIQUES Preparation of histological sections In order to
... Total preparations In some cases the tissue to be examined is a very thin membrane. In such cases the tissue does not need cutting on a microtome, but can be stained, mounted and examined directly. This is known as a total preparation. Total preparations are not as 2-dimensional as histological sect ...
... Total preparations In some cases the tissue to be examined is a very thin membrane. In such cases the tissue does not need cutting on a microtome, but can be stained, mounted and examined directly. This is known as a total preparation. Total preparations are not as 2-dimensional as histological sect ...
Isolation and Characterization of Cell Wall
... Chemical composition of the cell wall and outer membrane As evidenced by the chemical composition (Table 1) of unfractionated preparations, cell walls of Synechococcus sp. PCC 6307 contained proteins, lipopolysaccharide, polysaccharide, lipids and carotenoids as well as the peptidoglycan-specific co ...
... Chemical composition of the cell wall and outer membrane As evidenced by the chemical composition (Table 1) of unfractionated preparations, cell walls of Synechococcus sp. PCC 6307 contained proteins, lipopolysaccharide, polysaccharide, lipids and carotenoids as well as the peptidoglycan-specific co ...
Licensed to: iChapters User
... as trees and humans, are structural and functional aggregates of trillions of cells (multi means “many”). In the simpler multicellular forms of life—for example, a sponge—the cells of the organism are all similar. However, more complex organisms, such as humans, have many different kinds of cells, s ...
... as trees and humans, are structural and functional aggregates of trillions of cells (multi means “many”). In the simpler multicellular forms of life—for example, a sponge—the cells of the organism are all similar. However, more complex organisms, such as humans, have many different kinds of cells, s ...
Mechanistic investigation into the actions of taurine on beta cells
... Taurine (2-aminoethanesulphonic acid) is a semi-essential amino acid, which is not incorporated into proteins. In mammalian tissues, taurine is ubiquitous and is the most abundant free amino acid in the heart, retina, skeletal muscle, brain, and leukocytes, exhibiting multiple functions and tissue-p ...
... Taurine (2-aminoethanesulphonic acid) is a semi-essential amino acid, which is not incorporated into proteins. In mammalian tissues, taurine is ubiquitous and is the most abundant free amino acid in the heart, retina, skeletal muscle, brain, and leukocytes, exhibiting multiple functions and tissue-p ...
Cellular differentiation
In developmental biology, cellular differentiation isa cell changes from one cell type to another. Most commonly this is a less specialized type becoming a more specialized type, such as during cell growth. Differentiation occurs numerous times during the development of a multicellular organism as it changes from a simple zygote to a complex system of tissues and cell types. Differentiation continues in adulthood as adult stem cells divide and create fully differentiated daughter cells during tissue repair and during normal cell turnover. Some differentiation occurs in response to antigen exposure. Differentiation dramatically changes a cell's size, shape, membrane potential, metabolic activity, and responsiveness to signals. These changes are largely due to highly controlled modifications in gene expression and are the study of epigenetics. With a few exceptions, cellular differentiation almost never involves a change in the DNA sequence itself. Thus, different cells can have very different physical characteristics despite having the same genome.A cell that can differentiate into all cell types of the adult organism is known as pluripotent. Such cells are called embryonic stem cells in animals and meristematic cells in higher plants. A cell that can differentiate into all cell types, including the placental tissue, is known as totipotent. In mammals, only the zygote and subsequent blastomeres are totipotent, while in plants many differentiated cells can become totipotent with simple laboratory techniques. In cytopathology, the level of cellular differentiation is used as a measure of cancer progression. ""Grade"" is a marker of how differentiated a cell in a tumor is.