11 May 2017 17:07 E.S.T. ASGCT Posters 2017
... Genome engineering with programmable nucleases such as CRISPR-Cas or TALEN has revolutionized biological research and has broad-based therapeutic applications. However, for clinical use, it is essential to understand the totality of genome-modifying effects of these nucleases as deleterious off-targ ...
... Genome engineering with programmable nucleases such as CRISPR-Cas or TALEN has revolutionized biological research and has broad-based therapeutic applications. However, for clinical use, it is essential to understand the totality of genome-modifying effects of these nucleases as deleterious off-targ ...
sites of protein synthesis in nucleoli of root meristematic cells of
... nature of nucleolar proteins which were being synthesized during the 5-min exposure to [3H]arginine is not known. They could be any of the four types of proteins which biochemists claim are present in both plant and animal nucleoli, since all four have been shown to contain arginine and become label ...
... nature of nucleolar proteins which were being synthesized during the 5-min exposure to [3H]arginine is not known. They could be any of the four types of proteins which biochemists claim are present in both plant and animal nucleoli, since all four have been shown to contain arginine and become label ...
Cytotoxicity and inhibition of platelet aggregation
... reported to exert a multiplicity of effects on biological organisms including the induction or inhibition of platelet aggregation [4–6]. These enzymes also exhibit antibacterial and antiviral properties, abolishing the growth of both Gram-positive and -negative prokaryotes as well as HIV [7–9]. Furt ...
... reported to exert a multiplicity of effects on biological organisms including the induction or inhibition of platelet aggregation [4–6]. These enzymes also exhibit antibacterial and antiviral properties, abolishing the growth of both Gram-positive and -negative prokaryotes as well as HIV [7–9]. Furt ...
Cell Division Activity during Apical Hook
... (Fig. 3B). The apical part contained 1.7 times more cells compared with the basal part. In addition, Cyc1B-GUS was predominantly expressed at the apical part of the hook, as determined by the number of cells expressing GUS (Fig. 2D). These results indicate that the differential growth along the apic ...
... (Fig. 3B). The apical part contained 1.7 times more cells compared with the basal part. In addition, Cyc1B-GUS was predominantly expressed at the apical part of the hook, as determined by the number of cells expressing GUS (Fig. 2D). These results indicate that the differential growth along the apic ...
as Adobe PDF - Edinburgh Research Explorer
... Dual origin of infundibular cells from anterior floor plate To address whether anterior fp cells contribute to the infundibulum, we performed fate-mapping studies in chick embryos, tracing cells from st 9 until E7 (Fig. 1A-C), when the infundibulum is structurally distinct (Fig. 1D). DiI-labelled ce ...
... Dual origin of infundibular cells from anterior floor plate To address whether anterior fp cells contribute to the infundibulum, we performed fate-mapping studies in chick embryos, tracing cells from st 9 until E7 (Fig. 1A-C), when the infundibulum is structurally distinct (Fig. 1D). DiI-labelled ce ...
Biology - trinity
... BIO.A.2.1 Describe how the unique properties of water support life on Earth. BIO.A.2.2 Describe and interpret relationships between structure and function at various levels of biochemical organization (i.e., atoms, molecules, and macromolecules). BIO.A.2.3 Explain how enzymes regulate biochemical re ...
... BIO.A.2.1 Describe how the unique properties of water support life on Earth. BIO.A.2.2 Describe and interpret relationships between structure and function at various levels of biochemical organization (i.e., atoms, molecules, and macromolecules). BIO.A.2.3 Explain how enzymes regulate biochemical re ...
Domain conservation in several volvocalean cell wall - UvA-DARE
... carry ProProXYLys repeats. However, as the number of characterized H R G P s has increased, considerable variation has been found in the canonical repeats, and there has been a growing tendency to emphasize the novelty of these variants (e.g., monocot vs. dicot extensins). Recently, Kieliszewski and ...
... carry ProProXYLys repeats. However, as the number of characterized H R G P s has increased, considerable variation has been found in the canonical repeats, and there has been a growing tendency to emphasize the novelty of these variants (e.g., monocot vs. dicot extensins). Recently, Kieliszewski and ...
mRNA translation during oocyte maturation plays a key role in
... (such as maternal proteins and coding RNAs). As indicated in the results, we found unexpectedly that the number of PGCs in gonadotropin stimulated embryos was almost twice as many as that in the unstimulated ones, suggesting that gonadotropin increases PGC numbers. As there are evidence (Wasserman e ...
... (such as maternal proteins and coding RNAs). As indicated in the results, we found unexpectedly that the number of PGCs in gonadotropin stimulated embryos was almost twice as many as that in the unstimulated ones, suggesting that gonadotropin increases PGC numbers. As there are evidence (Wasserman e ...
AN ELECTRON-MICROSCOPE STUDY OF NATURALLY
... usually bent and segmented as if composed of shorter units. Thickness was variable, but in general was about 30% less than that of pili a and some micrographs (fig. 2b) showed these fibrils as a continuation of normal pili. Because it is not yet clear whether they are structurally related to pili a ...
... usually bent and segmented as if composed of shorter units. Thickness was variable, but in general was about 30% less than that of pili a and some micrographs (fig. 2b) showed these fibrils as a continuation of normal pili. Because it is not yet clear whether they are structurally related to pili a ...
Primary Mediastinal Seminomas: Evidence of Single KIT
... gonads. Alternatively, this effective lack of KIT expression may in turn lead to abnormal growth regulation and possible malignant transformation, thereby producing a more aggressive tumor (Strohmeyer et al, 1995). Finally, the source of antibodies used in the previously identified studies was diffe ...
... gonads. Alternatively, this effective lack of KIT expression may in turn lead to abnormal growth regulation and possible malignant transformation, thereby producing a more aggressive tumor (Strohmeyer et al, 1995). Finally, the source of antibodies used in the previously identified studies was diffe ...
Plk4/SAK/ZYG-1 in the regulation of centriole
... Active Plk4/SAK kinase is present at duplicating mother centrioles during G1/S and the protein levels increase at both centrioles into mitosis [29]. In addition to centriole localization, Plk4/SAK protein levels are regulated and, when aberrant, centriole assembly is either amplified or decreased co ...
... Active Plk4/SAK kinase is present at duplicating mother centrioles during G1/S and the protein levels increase at both centrioles into mitosis [29]. In addition to centriole localization, Plk4/SAK protein levels are regulated and, when aberrant, centriole assembly is either amplified or decreased co ...
arc6, an extreme chloroplast division mutant of Arabidopsis also
... Fig. 2E sequential sections are shown through a representative cell from the arc6 apical meristem (Fig. 2C) and clearly show how small individual arc6 proplastid profiles contribute to a single large proplastid with a complex three-dimensional morphology. arc6 proplastids have many surface protubera ...
... Fig. 2E sequential sections are shown through a representative cell from the arc6 apical meristem (Fig. 2C) and clearly show how small individual arc6 proplastid profiles contribute to a single large proplastid with a complex three-dimensional morphology. arc6 proplastids have many surface protubera ...
Craniofacial Development and the Evolution of the Vertebrates: the
... eye muscles around the eye (e). Some of the somitederived myotomal cells express the Lbx1 gene, which is necessary for the ventral migration of these cells to form the hypobranchial and limb (fin) muscles. (C) Cephalic neural crest cells. The crest cells (hatched red) in the head migrate along the d ...
... eye muscles around the eye (e). Some of the somitederived myotomal cells express the Lbx1 gene, which is necessary for the ventral migration of these cells to form the hypobranchial and limb (fin) muscles. (C) Cephalic neural crest cells. The crest cells (hatched red) in the head migrate along the d ...
The Putative Arabidopsis Arp2/3 Complex
... eukaryotes, but its biological functions are not well understood in multicellular organisms. The model plant Arabidopsis provides many advantages for genetic dissection of the function of this conserved actin-nucleating machinery, yet the existence of this complex in plants has not been determined. ...
... eukaryotes, but its biological functions are not well understood in multicellular organisms. The model plant Arabidopsis provides many advantages for genetic dissection of the function of this conserved actin-nucleating machinery, yet the existence of this complex in plants has not been determined. ...
FGF-dependent midline-derived progenitor cells in hypothalamic
... Dual origin of infundibular cells from anterior floor plate To address whether anterior fp cells contribute to the infundibulum, we performed fate-mapping studies in chick embryos, tracing cells from st 9 until E7 (Fig. 1A-C), when the infundibulum is structurally distinct (Fig. 1D). DiI-labelled ce ...
... Dual origin of infundibular cells from anterior floor plate To address whether anterior fp cells contribute to the infundibulum, we performed fate-mapping studies in chick embryos, tracing cells from st 9 until E7 (Fig. 1A-C), when the infundibulum is structurally distinct (Fig. 1D). DiI-labelled ce ...
Churchill, a Zinc Finger Transcriptional Activator, Regulates the
... within 2 hr, but unless cells are exposed to other (unknown) signals, this expression is lost and cells revert to an epidermal fate. We have also found that 5 hr exposure to either organizer-derived (Hensen’s node) signals or to FGF8 are required to sensitize cells to BMP antagonists, which then sta ...
... within 2 hr, but unless cells are exposed to other (unknown) signals, this expression is lost and cells revert to an epidermal fate. We have also found that 5 hr exposure to either organizer-derived (Hensen’s node) signals or to FGF8 are required to sensitize cells to BMP antagonists, which then sta ...
Involvement of retinoic acid-inducible gene- BEAS-2B cells
... expression of RIG-I mRNA induced by IFN-c. IFN-c-induced RIG-I expression was also demonstrated in NCI-H292 cells by Western blot analysis (fig. 6d and e). DISCUSSION T-lymphocytes play a central role in the regulation of immune responses through the secretion of different sets of cytokines. T-helpe ...
... expression of RIG-I mRNA induced by IFN-c. IFN-c-induced RIG-I expression was also demonstrated in NCI-H292 cells by Western blot analysis (fig. 6d and e). DISCUSSION T-lymphocytes play a central role in the regulation of immune responses through the secretion of different sets of cytokines. T-helpe ...
Polysialic acid controls NCAM signals at cell–cell contacts to
... (red), actin staining with FITC–phalloidin (green) and nuclear counterstain with DAPI (blue) in LS cell transfectants expressing polysialylated NCAM-140 (LSAM1PST). In B, cells were incubated for 30 minutes with 200 ng/ml endoneuramindase (endo). (C) Evaluation of peripheral focal adhesions per cell ...
... (red), actin staining with FITC–phalloidin (green) and nuclear counterstain with DAPI (blue) in LS cell transfectants expressing polysialylated NCAM-140 (LSAM1PST). In B, cells were incubated for 30 minutes with 200 ng/ml endoneuramindase (endo). (C) Evaluation of peripheral focal adhesions per cell ...
Wingless mutation in Drosophila melanogaster
... again recently become a favoured organism for the study of the genetic basis of development. In order to understand the genetic programming of development, we could classify genes of Drosophila into those with a controlling role and those with a subsidiary function. The likely candidates for control ...
... again recently become a favoured organism for the study of the genetic basis of development. In order to understand the genetic programming of development, we could classify genes of Drosophila into those with a controlling role and those with a subsidiary function. The likely candidates for control ...
Stat3 and MMP7 Contribute to Pancreatic Ductal Adenocarcinoma
... expression level of inflammation-associated cytokine is also noted to be lower Kras-driven ductal metaplasia of Stat3 depleted mice. In addition, Stat3 supports MMP7 expression during Kras-driven PanIN development following caerulin-induced pancreatitis. Using MMP7 knockout mice, the investigator fu ...
... expression level of inflammation-associated cytokine is also noted to be lower Kras-driven ductal metaplasia of Stat3 depleted mice. In addition, Stat3 supports MMP7 expression during Kras-driven PanIN development following caerulin-induced pancreatitis. Using MMP7 knockout mice, the investigator fu ...
Linker histone H1 in early mouse embryogenesis
... increases during terminal cell differentiation (Rousseau et al., 1992), and its overproduction results in a decrease of transcriptional activity (Brown et al., 1997). However, the immediate future of the differentiated oocyte is to become a totipotent cell following fertilization or parthenogenetic ...
... increases during terminal cell differentiation (Rousseau et al., 1992), and its overproduction results in a decrease of transcriptional activity (Brown et al., 1997). However, the immediate future of the differentiated oocyte is to become a totipotent cell following fertilization or parthenogenetic ...
Microreviews in Cell and Molecular Biology
... Dynamin A is a representative of the family of dynamin related proteins. With the results we can come to the conclusion that D. discoideum protein shares features, like the tripartite GTP- binding motif, the consensus pattern LPRGSGIVTR, and a high degree of sequence identity. Dynamin B is a nuclear ...
... Dynamin A is a representative of the family of dynamin related proteins. With the results we can come to the conclusion that D. discoideum protein shares features, like the tripartite GTP- binding motif, the consensus pattern LPRGSGIVTR, and a high degree of sequence identity. Dynamin B is a nuclear ...
Patterns of Collective Bacterial Motion in Microfluidic Devices
... trient availability or faster biochemical signal propagation due to enhanced diffusion). Interestingly, pattern formation has also been observed for suspensions of non-active agents.8 Settling of colloid particles under gravitation is an actively studied field of statistical mechanics, and computat ...
... trient availability or faster biochemical signal propagation due to enhanced diffusion). Interestingly, pattern formation has also been observed for suspensions of non-active agents.8 Settling of colloid particles under gravitation is an actively studied field of statistical mechanics, and computat ...
Ubiquitination and sumoylation of the HTLV-2 κB activity: a
... encompassing the four C-terminal amino acids responsible for the binding to several PDZ domain-containing proteins [16-18]. In addition, some HTLV-2 subtypes express shorter versions of Tax-2 (namely Tax-2A and Tax-2CG) which, contrary to Tax-2B, do not functionally inactivate p53 [10,19]. Recent st ...
... encompassing the four C-terminal amino acids responsible for the binding to several PDZ domain-containing proteins [16-18]. In addition, some HTLV-2 subtypes express shorter versions of Tax-2 (namely Tax-2A and Tax-2CG) which, contrary to Tax-2B, do not functionally inactivate p53 [10,19]. Recent st ...
Cellular differentiation
In developmental biology, cellular differentiation isa cell changes from one cell type to another. Most commonly this is a less specialized type becoming a more specialized type, such as during cell growth. Differentiation occurs numerous times during the development of a multicellular organism as it changes from a simple zygote to a complex system of tissues and cell types. Differentiation continues in adulthood as adult stem cells divide and create fully differentiated daughter cells during tissue repair and during normal cell turnover. Some differentiation occurs in response to antigen exposure. Differentiation dramatically changes a cell's size, shape, membrane potential, metabolic activity, and responsiveness to signals. These changes are largely due to highly controlled modifications in gene expression and are the study of epigenetics. With a few exceptions, cellular differentiation almost never involves a change in the DNA sequence itself. Thus, different cells can have very different physical characteristics despite having the same genome.A cell that can differentiate into all cell types of the adult organism is known as pluripotent. Such cells are called embryonic stem cells in animals and meristematic cells in higher plants. A cell that can differentiate into all cell types, including the placental tissue, is known as totipotent. In mammals, only the zygote and subsequent blastomeres are totipotent, while in plants many differentiated cells can become totipotent with simple laboratory techniques. In cytopathology, the level of cellular differentiation is used as a measure of cancer progression. ""Grade"" is a marker of how differentiated a cell in a tumor is.