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Chapter 1 - Utrecht University Repository
Chapter 1 - Utrecht University Repository

... isolated, which were differentially associated with rab4, rab11 and transferrin receptor (Sheff et al., 1999). In immunoisolation experiments the recycling endosome was isolated. This compartment contains molecules involved in the regulation of transferrin recycling, as rab4, rab11 and cellubrevin a ...
Challenges to our current view on chloroplasts
Challenges to our current view on chloroplasts

... changes in their architecture and physiology. Furthermore, these changes occur alongside differentiation processes of cells and organs during plant development (reviewed, e.g., by Reski, 1994). Two major lines of modifications appear to be important: (1) during evolution of flowering plants with the ...
When cells get stressed: an integrative view of cellular senescence
When cells get stressed: an integrative view of cellular senescence

... Senescence is thus induced at a midway point of telomere shortening. This provokes questions regarding the nature of the molecular changes that occur at the telomeres at this point, and the manner by which these changes activate the senescence program. Different models have been proposed to explain ...
Linköping University Post Print Neutralizing activity and cellular immune
Linköping University Post Print Neutralizing activity and cellular immune

... C57BL/6 mice were bred and kept at the animal facility at MTC, Karolinska Institutet. Mice were immunized i.p, s.c, i.m. or i.n. with either apoptotic HIV-1/MuLV infected or MuLV infected cells as previously reported [18] . In brief, human CEM-1B cells containing the complete murine leukaemia virus ...
A cAMP Switch in the Attractab Versus Repellent Response
A cAMP Switch in the Attractab Versus Repellent Response

... Electrophysiology. Whole-cell perforated patch recordings30 from two to three hippocampal neurons were performed simultaneously, using amphotericin B (150 g/ml, Calbiochem) for perforation. The micropipettes were made from borosilicate glass capillaries (VWR), with a resistance in the range of 1.5– ...
Guard cells in albino leaf patches do not respond to
Guard cells in albino leaf patches do not respond to

... (Oelmüller & Mohr 1986; Strand et al. 2003; McCormac & Terry 2004a). In addition, Nf affects the transcription rates of a large number of other genes encoding proteins that are not transported to chloroplasts (McCormac & Terry 2004b). The latter changes in gene transcription, to some extent, may be ...
Cardiac Stem Cells and Mechanisms of Myocardial Regeneration
Cardiac Stem Cells and Mechanisms of Myocardial Regeneration

... with scar formation during wound healing (269). Moreover, the inflammatory response is attenuated; a low number of poorly differentiated inflammatory cells accumulate in the region of damage, and the growth factors present at the site of healing in embryos are different from those in the adult organ ...
BIOLOGY I - one credit - COMPETENCIES AND OBJECTIVES
BIOLOGY I - one credit - COMPETENCIES AND OBJECTIVES

... hydrogen bonding, adhesion, cohesion, and expansion upon freezing). (DOK 2) c. Classify solutions as acidic, basic, or neutral and relate the significance of the pH scale on an organism’s survival (e.g., consequences of having different concentrations of hydrogen and hydroxide ions). (DOK 2) d. Comp ...
Development of neural tube basal lamina during neurulation and
Development of neural tube basal lamina during neurulation and

... stage; Fig. 8A,B). There is no BL over the NC cells that are still in the neural tube (Fig. 8C,D), nor is there any BL material on those that are actively migrating (not shown). However, the BL is starting to be deposited under the dorsoventral NE (Fig. 8E, arrowheads). In addition to the progressiv ...
chiasma formation occurs at or following mid-prophase
chiasma formation occurs at or following mid-prophase

... (plate I, fig. B) is approximately 2 days. Labelled cells appeared in pachytene at day 3 (plate I, fig. C). It requires approximately 5 days for cells labelled during pre-meiotic S to reach diplotene (plate I, fig. D). Chiasma frequencies are presented in text-figs. 1 and 2. The normal mean chiasma ...
dextran by suspension-cultured plant cells
dextran by suspension-cultured plant cells

... protoplasts. This phenomenon was particularly apparent in the large vacuoles of elongate cells (Fig. 1G). Intense fluorescence was also observed within vesicles or small vacuoles located in the cytoplasm of both cells and protoplasts (Fig. 1F-K). Further to this, fluorescence was detected within an ...
PDF
PDF

... stage; Fig. 8A,B). There is no BL over the NC cells that are still in the neural tube (Fig. 8C,D), nor is there any BL material on those that are actively migrating (not shown). However, the BL is starting to be deposited under the dorsoventral NE (Fig. 8E, arrowheads). In addition to the progressiv ...
Heart Valve Tissue Engineering: Concepts, Approaches, Progress
Heart Valve Tissue Engineering: Concepts, Approaches, Progress

... Ross valves transplanted from the pulmonary-to-aortic position in an individual) presently are viable,120 but the Ross procedure is technically difficult, risky, only serves a small patient subset, and has controversial results, including uncertainty over whether the grafts will grow commensurate wi ...
The Regulation of S Phase Initiation by p27Kip1 in NIH3T3 Cells
The Regulation of S Phase Initiation by p27Kip1 in NIH3T3 Cells

... was corrected with a uniformly stained specimen; and the DAPI image was utilized for thresholding with the Metamorph software (Universal Imaging) to identify nuclei of individual cells. The fluorescence in this region was then quantitated for each fluorochrome. The cell cycle related expression of e ...
Biology I Framework Competencies
Biology I Framework Competencies

... upon freezing). (DOK 2) c. Classify solutions as acidic, basic, or neutral and relate the significance of the pH scale to an organism’s survival (e.g., consequences of having different concentrations of hydrogen and hydroxide ions). (DOK 2) d. Compare and contrast the structure, properties, and prin ...
What is bad in cancer is good in the embryo: Importance of EMT in
What is bad in cancer is good in the embryo: Importance of EMT in

... components, loss of apicobasal polarity and rupture of the basement membrane, which then leads to mesenchymal motility. However, these functions overlap and the cell also needs to maintain multipotency, survival and overall adhesive integrity of the source tissue [27]. In this review we focus on wha ...
Nitrogen gas flushing can be bactericidal: the temperature
Nitrogen gas flushing can be bactericidal: the temperature

... bacteria in raw and pasteurized milk, respectively. In previous studies, nitrogen (N2 ) gas flushing treatments of raw and pasteurized milk at cold chain-temperatures inhibited bacterial spoilage and highlighted different susceptibilities to the N2 treatment with the exclusion of certain bacterial t ...
PDF - Oxford Academic
PDF - Oxford Academic

... 2156 | Ábrahám et al. were first identified from endosperm and seedling libraries (Grafi et al., 1996; Xie et al., 1996; Ach et al., 1997). The list of cloned plant RBR genes is continuously expanding, and the phylogenetic tree reveals significant differences between dicot and monocot plant speci ...
Complex Receptive Fields in Primary Visual Cortex
Complex Receptive Fields in Primary Visual Cortex

... Ringach 2002, for review). To give an example of the confusion, cell B of Figure 2 could probably be classified by different authors as complex (e.g., Hubel and Wiesel 1962), simple (e.g., Skottun and others 1991a), S1 (e.g., Schiller and others 1976; Martin and Whitteridge 1984; Jones and Palmer 19 ...
chapt01_lecture
chapt01_lecture

... body works to maintain life ...
Relating the mechanics of the primary plant cell
Relating the mechanics of the primary plant cell

... can occur in protoplasmic or newly secreted RG-IIs but not in those already existing in the cell wall (Chormova et al., 2014). Borate cross-linking is essential for successful expansive growth of cells by providing the cell wall with proper mechanical strength. In the cell wall of Arabidopsis rosett ...
Contributions by members of the TGFbeta superfamily to lens
Contributions by members of the TGFbeta superfamily to lens

... receptors is modulated by co-receptors and numerous extracellular ...
Amniotic membrane modulates innate immune response inhibiting
Amniotic membrane modulates innate immune response inhibiting

... b, and by the other hand activate the nuclear factor-kB (NFkB) favoring the production of proinflammatory cytokines (Kawai et al., 2005; Hirata et al., 2007; Kanneganti, 2010). In this context, it has been described that NFkB is an important molecule in cell activation process throughout maintaining ...
PrimeFlow™ RNA Assay Technology Validation Paper
PrimeFlow™ RNA Assay Technology Validation Paper

... PrimeFlow™ RNA Assay reveals the dynamics of RNA and protein expression within individual cells, facilitating unprecedented analysis of their correlation as the cells change over time or in response to stimulation. This novel assay uses fluorescent in situ hybridization (FISH) to enable simultaneous ...
Colloidal Gold-Mediated Delivery of Bleomycin for Improved
Colloidal Gold-Mediated Delivery of Bleomycin for Improved

... to tumors while causing minimum side effects. Many of the side effects of anticancer drugs are caused because of their nonspecific attack on all rapidly dividing cells. Hence, GNPs can be used to resolve certain limitations in chemotherapy such as side effects through targeting and effective loading ...
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Cellular differentiation



In developmental biology, cellular differentiation isa cell changes from one cell type to another. Most commonly this is a less specialized type becoming a more specialized type, such as during cell growth. Differentiation occurs numerous times during the development of a multicellular organism as it changes from a simple zygote to a complex system of tissues and cell types. Differentiation continues in adulthood as adult stem cells divide and create fully differentiated daughter cells during tissue repair and during normal cell turnover. Some differentiation occurs in response to antigen exposure. Differentiation dramatically changes a cell's size, shape, membrane potential, metabolic activity, and responsiveness to signals. These changes are largely due to highly controlled modifications in gene expression and are the study of epigenetics. With a few exceptions, cellular differentiation almost never involves a change in the DNA sequence itself. Thus, different cells can have very different physical characteristics despite having the same genome.A cell that can differentiate into all cell types of the adult organism is known as pluripotent. Such cells are called embryonic stem cells in animals and meristematic cells in higher plants. A cell that can differentiate into all cell types, including the placental tissue, is known as totipotent. In mammals, only the zygote and subsequent blastomeres are totipotent, while in plants many differentiated cells can become totipotent with simple laboratory techniques. In cytopathology, the level of cellular differentiation is used as a measure of cancer progression. ""Grade"" is a marker of how differentiated a cell in a tumor is.
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