Primary cultures of unilocular fat cells
... coated with Sakura NR-M2 emulsion (Konishiroku, Tokyo) and exposed for 2 weeks before being developed using Konidol X (Konishiroku). The specimens were then stained with oil red 0 and hematoxylin. To examine the specific incorporation of H-thymidine into DNA, two control procedures were followed. Fi ...
... coated with Sakura NR-M2 emulsion (Konishiroku, Tokyo) and exposed for 2 weeks before being developed using Konidol X (Konishiroku). The specimens were then stained with oil red 0 and hematoxylin. To examine the specific incorporation of H-thymidine into DNA, two control procedures were followed. Fi ...
Targeting cell surface receptors for axon regeneration in the central
... the extrinsic advocates who study the limiting factors found in the lesion environment, while the intrinsic advocates who focus on factors found within the neurons. Perhaps cell surface receptors can serve as a link between these two groups as the presence of a correct receptor on the cell surface i ...
... the extrinsic advocates who study the limiting factors found in the lesion environment, while the intrinsic advocates who focus on factors found within the neurons. Perhaps cell surface receptors can serve as a link between these two groups as the presence of a correct receptor on the cell surface i ...
BMP Induction of Id Proteins Suppresses Differentiation
... and allows self-renewal in LIF alone. Upon LIF withdrawal, Id-expressing ES cells differentiate but do not give rise to neural lineages. We conclude that blockade of lineage-specific transcription factors by Id proteins enables the self-renewal response to LIF/STAT3. Introduction Stem cells may be d ...
... and allows self-renewal in LIF alone. Upon LIF withdrawal, Id-expressing ES cells differentiate but do not give rise to neural lineages. We conclude that blockade of lineage-specific transcription factors by Id proteins enables the self-renewal response to LIF/STAT3. Introduction Stem cells may be d ...
Beginnings of a Good Apoptotic Meal: The Find-Me and
... LPC in the serum or plasma (100 mM), it is unclear how LPC can set up a gradient to attract phagocytes specifically to the proximity of apoptotic cells. Although high concentration of LPC could be present locally near a dying cell and thereby still provide the gradient, higher LPC concentrations cau ...
... LPC in the serum or plasma (100 mM), it is unclear how LPC can set up a gradient to attract phagocytes specifically to the proximity of apoptotic cells. Although high concentration of LPC could be present locally near a dying cell and thereby still provide the gradient, higher LPC concentrations cau ...
silicone rubber in a parallel plate flow chamber in the absence and
... surface. The initial colonizing organisms are of importance as they play a pivotal role in the linking of the final biofilm to the implant surface (Busscher et al., ...
... surface. The initial colonizing organisms are of importance as they play a pivotal role in the linking of the final biofilm to the implant surface (Busscher et al., ...
silicone rubber in a parallel plate flow chamber in the
... surface. The initial colonizing organisms are of importance as they play a pivotal role in the linking of the final biofilm to the implant surface (Busscher et al., ...
... surface. The initial colonizing organisms are of importance as they play a pivotal role in the linking of the final biofilm to the implant surface (Busscher et al., ...
Class I MHC Molecule Protein in Association with an Allogeneic
... containing the entire sequence of p2Ca (5). Both peptides are derived from a ubiquitous intracellular protein, oxoglutarate dehydrogenase (OGDH) (5, 6). A synthetic p2Cb peptide was digested in vitro with cellular extracts containing proteosomes and was found to produce active fragments, suggesting ...
... containing the entire sequence of p2Ca (5). Both peptides are derived from a ubiquitous intracellular protein, oxoglutarate dehydrogenase (OGDH) (5, 6). A synthetic p2Cb peptide was digested in vitro with cellular extracts containing proteosomes and was found to produce active fragments, suggesting ...
PDF
... two different phases, there is a 30min period of uncertainty regarding the actual transition from one phase to the next. (2) Whereas cells were judged to have been in G2 or M phase on the basis of criteria that reflect the state of the cell at the time of fixation, cells were judged to have been in ...
... two different phases, there is a 30min period of uncertainty regarding the actual transition from one phase to the next. (2) Whereas cells were judged to have been in G2 or M phase on the basis of criteria that reflect the state of the cell at the time of fixation, cells were judged to have been in ...
Engagement of the inhibitory receptor CD158a
... supertype alleles) was derived from tumor infiltrating lymphocytes of a renal tumor. This tumor contained 2% of CD158a+ CD8+ T cells and VB13.1+T cells corresponded to 16% of the CD158a+ T cells (data not shown). This CTL is HLA-A2 restricted and recognized an antigen expressed by tumor cells of dif ...
... supertype alleles) was derived from tumor infiltrating lymphocytes of a renal tumor. This tumor contained 2% of CD158a+ CD8+ T cells and VB13.1+T cells corresponded to 16% of the CD158a+ T cells (data not shown). This CTL is HLA-A2 restricted and recognized an antigen expressed by tumor cells of dif ...
Dermatopathology: An abridged compendium of words
... by a tad of serum and/or a hint of spongiosis. Both the systematized and palmoplantar expressions of focal acantholytic dyskeratosis are representative of a type of epidermal nevus. In contradistinction to focal acantholytic dyskeratosis is confluent acantholytic dyskeratosis, which manifests itself ...
... by a tad of serum and/or a hint of spongiosis. Both the systematized and palmoplantar expressions of focal acantholytic dyskeratosis are representative of a type of epidermal nevus. In contradistinction to focal acantholytic dyskeratosis is confluent acantholytic dyskeratosis, which manifests itself ...
Summer 2012
... development, Lucia™, a new secreted luciferase optimized to advance cell based reporter technology. Luciferases encompass a wide range of enzymes used for bioluminescence, the emission of light produced by a living organism. Luciferases are highly prized bioindicators for life science research and d ...
... development, Lucia™, a new secreted luciferase optimized to advance cell based reporter technology. Luciferases encompass a wide range of enzymes used for bioluminescence, the emission of light produced by a living organism. Luciferases are highly prized bioindicators for life science research and d ...
Lectures 1 and 2
... Proteins can bind at the ends of N-linked and O-linked chains, and typically have binding pockets ...
... Proteins can bind at the ends of N-linked and O-linked chains, and typically have binding pockets ...
The roles of microtubules in tropisms
... Microtubules have also been linked to organizational events associated with the plasma membrane. Although speculative, one possibility is that microtubules influence tropisms by positioning regulatory proteins and/or complexes in the plasma membrane. Two possible mechanisms by which microtubules coul ...
... Microtubules have also been linked to organizational events associated with the plasma membrane. Although speculative, one possibility is that microtubules influence tropisms by positioning regulatory proteins and/or complexes in the plasma membrane. Two possible mechanisms by which microtubules coul ...
Fc Macrophages via the ERK Pathway Receptor Cross-linking Stimulates Cell Proliferation of *
... Biosciences (San Jose, CA). The secondary horseradish peroxidase-conjugated anti-rabbit (NA 934V) and mouse (NA 931V) antibodies were obtained from Amersham Biosciences (Piscataway, NJ). The primary and secondary antibodies were used at the manufacturer-recommended dilutions for Western blot. Cultur ...
... Biosciences (San Jose, CA). The secondary horseradish peroxidase-conjugated anti-rabbit (NA 934V) and mouse (NA 931V) antibodies were obtained from Amersham Biosciences (Piscataway, NJ). The primary and secondary antibodies were used at the manufacturer-recommended dilutions for Western blot. Cultur ...
Muscular System Overview of Muscle Tissues • Types of Muscle
... loosely knit sweater) by ion pumps (i.e., an integral protein of a cell membrane that actively transports ions from one side of the membrane to the other). o The active transport (i.e., the movement of materials across a cell membrane against a concentration gradient. This process uses a carrier pro ...
... loosely knit sweater) by ion pumps (i.e., an integral protein of a cell membrane that actively transports ions from one side of the membrane to the other). o The active transport (i.e., the movement of materials across a cell membrane against a concentration gradient. This process uses a carrier pro ...
Membrane Structure and Function Chapter 7 Biology
... – Intercellular joining – Attachment to the cytoskeleton and extracellular matrix (ECM) ...
... – Intercellular joining – Attachment to the cytoskeleton and extracellular matrix (ECM) ...
Using intrinsically fluorescent proteins for plant cell
... predicted location, but the construct can alter in vivo activity (Wang et al., 2004). In establishing the fidelity of the IFP chimera, consideration must be given to the activity of the chimeric protein as well as to its localization. The fidelity of the observed localization pattern is important an ...
... predicted location, but the construct can alter in vivo activity (Wang et al., 2004). In establishing the fidelity of the IFP chimera, consideration must be given to the activity of the chimeric protein as well as to its localization. The fidelity of the observed localization pattern is important an ...
Developmental neurobiology of hydra, a model animal of cnidarians1
... Introduction to the nerve net of hydra: unique features of the diffuse nervous system The freshwater coelenterate hydra has a simple body plan. Like all coelenterates, it is a diploblastic animal: it is composed of two layers of epithelial cells, the endoderm and the ectoderm. The apical end is the ...
... Introduction to the nerve net of hydra: unique features of the diffuse nervous system The freshwater coelenterate hydra has a simple body plan. Like all coelenterates, it is a diploblastic animal: it is composed of two layers of epithelial cells, the endoderm and the ectoderm. The apical end is the ...
Extracellular matrix
In biology, the extracellular matrix (ECM) is a collection of extracellular molecules secreted by cells that provides structural and biochemical support to the surrounding cells. Because multicellularity evolved independently in different multicellular lineages, the composition of ECM varies between multicellular structures; however, cell adhesion, cell-to-cell communication and differentiation are common functions of the ECM.The animal extracellular matrix includes the interstitial matrix and the basement membrane. Interstitial matrix is present between various animal cells (i.e., in the intercellular spaces). Gels of polysaccharides and fibrous proteins fill the interstitial space and act as a compression buffer against the stress placed on the ECM. Basement membranes are sheet-like depositions of ECM on which various epithelial cells rest.The plant ECM includes cell wall components, like cellulose, in addition to more complex signaling molecules. Some single-celled organisms adopt multicelluar biofilms in which the cells are embedded in an ECM composed primarily of extracellular polymeric substances (EPS).