6.3. La supervivencia de las motoneuronas espinales de pollo mantenidas
... 37°C for 1 hr. The fluorescent signals were determined with a spectrofluorometer (Bio-Tek Instruments, Winooski, Vermont) at an excitation wavelength of 360 nm and an emission wavelength of 530 nm. Protease activity was expressed as the amount of cleaved substrate per microgram of protein per minute ( ...
... 37°C for 1 hr. The fluorescent signals were determined with a spectrofluorometer (Bio-Tek Instruments, Winooski, Vermont) at an excitation wavelength of 360 nm and an emission wavelength of 530 nm. Protease activity was expressed as the amount of cleaved substrate per microgram of protein per minute ( ...
Cyclin synthesis and degradation and the embryonic cell cycle
... experiments in which tissue culture cells in different stages of the cell cycle were fused to each other (Rao and Johnson, 1970). The dependent cycle can be viewed either as a traditional biochemical pathway where the product of each step acts as a substrate for the next, or as a series of essential ...
... experiments in which tissue culture cells in different stages of the cell cycle were fused to each other (Rao and Johnson, 1970). The dependent cycle can be viewed either as a traditional biochemical pathway where the product of each step acts as a substrate for the next, or as a series of essential ...
Note 19
... In hypotonic solution, there is a net movement of water into the cell by osmosis, rigid cell wall of microorganisms can prevent the cell from bursting or some microorganisms develop contractile vacuole to remove excess water. Most microorganisms can survive. In hypertonic solution, there is a net mo ...
... In hypotonic solution, there is a net movement of water into the cell by osmosis, rigid cell wall of microorganisms can prevent the cell from bursting or some microorganisms develop contractile vacuole to remove excess water. Most microorganisms can survive. In hypertonic solution, there is a net mo ...
CitA (citrate) and DcuS (C 4 -dicarboxylate) sensor kinases in
... efficiency similar to CitA of E. coli (Fig. 3B) indicating that citrate transport and citrate lyase ...
... efficiency similar to CitA of E. coli (Fig. 3B) indicating that citrate transport and citrate lyase ...
2008107-21541995JPR
... reconnected to another molecule in the wall network so as to allow both movement of cellulose microfibrils relative to one another and integration of new wall material into the cell wall. The archetype of this hypothesis was originally advanced by Albersheim as early as the mid1970s (Albersheim 1976 ...
... reconnected to another molecule in the wall network so as to allow both movement of cellulose microfibrils relative to one another and integration of new wall material into the cell wall. The archetype of this hypothesis was originally advanced by Albersheim as early as the mid1970s (Albersheim 1976 ...
Intrahepatic expression of the hepatic stellate cell marker ®broblast
... Other markers of human HSCs have been identi®ed that may identify phenotypic subsets more closely associated with the presence of ®brosis. Recently, we reported ®broblast activation protein (FAP) expression on a subset of activated human HSCs at the tissue-remodelling interface of cirrhotic liver (1 ...
... Other markers of human HSCs have been identi®ed that may identify phenotypic subsets more closely associated with the presence of ®brosis. Recently, we reported ®broblast activation protein (FAP) expression on a subset of activated human HSCs at the tissue-remodelling interface of cirrhotic liver (1 ...
Molecular differences between the rostral and caudal halves of the
... rostral and caudal half-sclerotome cells is not understood. However, the cells of the rostral half appear to express cytotactin (Tan et al. 1987), tenascin (Mackie et al. 1988) and butyrylcholinesterase activity (Layer et al. 1988), while the cells of the caudal half bind peanut lectin (Stern et al. ...
... rostral and caudal half-sclerotome cells is not understood. However, the cells of the rostral half appear to express cytotactin (Tan et al. 1987), tenascin (Mackie et al. 1988) and butyrylcholinesterase activity (Layer et al. 1988), while the cells of the caudal half bind peanut lectin (Stern et al. ...
Enzymes II: Regulation
... determined may be called primary isoenzymes. The different primary isoenzymes catalyze the same chemical reaction but may differ in their primary structure and kinetic properties. The tissue distribution of isoenzymes imparts distinctive properties and specific patterns of metabolism to organs of th ...
... determined may be called primary isoenzymes. The different primary isoenzymes catalyze the same chemical reaction but may differ in their primary structure and kinetic properties. The tissue distribution of isoenzymes imparts distinctive properties and specific patterns of metabolism to organs of th ...
Fungal and oomycete effectors – strategies to subdue a host
... without the help of the pathogen (Rafiqi et al., 2010; Kale et al., 2010). Recently, Kale et al. (2010) showed that the conserved RXLR motif from oomycetes and the RXLRlike motif from other fungi bind specifically to phospholipids, in particular phosphatidylinositol-3-phosphate (PI3P) on the surface ...
... without the help of the pathogen (Rafiqi et al., 2010; Kale et al., 2010). Recently, Kale et al. (2010) showed that the conserved RXLR motif from oomycetes and the RXLRlike motif from other fungi bind specifically to phospholipids, in particular phosphatidylinositol-3-phosphate (PI3P) on the surface ...
File
... system. The concentration gradient of each of these ions across the membrane helps determine the voltage of the membrane potential. Second, the quantitative importance of each of the ions in determining the voltage is proportional to the membrane permeability for that particular ion. That is, if th ...
... system. The concentration gradient of each of these ions across the membrane helps determine the voltage of the membrane potential. Second, the quantitative importance of each of the ions in determining the voltage is proportional to the membrane permeability for that particular ion. That is, if th ...
Membrane flow through Golgi compartments
... DOG medium (Fig. 3C). After 30-45 minutes of incubation in DOG medium, the perinuclear reticular structure stained by Helix pomatia disappeared (Fig. 3D,H,I) and a haze of fluorescence was spread throughout most of the area stained with an antibody against the ER marker protein disulphide isomerase ...
... DOG medium (Fig. 3C). After 30-45 minutes of incubation in DOG medium, the perinuclear reticular structure stained by Helix pomatia disappeared (Fig. 3D,H,I) and a haze of fluorescence was spread throughout most of the area stained with an antibody against the ER marker protein disulphide isomerase ...
Circadian Regulator CLOCK Is a Histone Acetyltransferase
... elements located in their promoter regions. The mPER and mCRY proteins then negatively feedback to repress their own transcription by acting on the mCLOCK: mBMAL1 complex. This negative-feedback regulation also drives gene expression cycles of a variety of circadian output genes (Lowrey and Takahash ...
... elements located in their promoter regions. The mPER and mCRY proteins then negatively feedback to repress their own transcription by acting on the mCLOCK: mBMAL1 complex. This negative-feedback regulation also drives gene expression cycles of a variety of circadian output genes (Lowrey and Takahash ...
PrionPPSatBlack
... Examples of questions that can be explored through structural analyses Some mutations have been associated with prion diseases. By mapping these onto structures, propose a hypothesis to explain this. Most of the variation seems to be in the Nterminal region. Is there homology to this region in othe ...
... Examples of questions that can be explored through structural analyses Some mutations have been associated with prion diseases. By mapping these onto structures, propose a hypothesis to explain this. Most of the variation seems to be in the Nterminal region. Is there homology to this region in othe ...
Cleavage Furrow Establishment—A Preliminary to Cylindrical
... has rarely furnished conclusive information on any of these subjects. Observations of division do not usually permit discrimination between cause and effect and may not reveal whether a structure or phenomenon present during division is causally related to it. That cell division depends upon the ope ...
... has rarely furnished conclusive information on any of these subjects. Observations of division do not usually permit discrimination between cause and effect and may not reveal whether a structure or phenomenon present during division is causally related to it. That cell division depends upon the ope ...
Mass Spectrometry Based Proteomics
... Deterioration of insulin secretion over time is the usual course in most type-2 diabetic patients, and many patients will end with more or less severe insulin deficiency after about 10 years of diabetes Hyperglycemia itself decreases insulin secretion and is implicated in the damage of beta cells ...
... Deterioration of insulin secretion over time is the usual course in most type-2 diabetic patients, and many patients will end with more or less severe insulin deficiency after about 10 years of diabetes Hyperglycemia itself decreases insulin secretion and is implicated in the damage of beta cells ...
Precursor of human adenovirus core polypeptide Mu targets the
... that Mu helps condense DNA in the virus core by means of a charged-based interaction between the nine arginine residues in Mu and the phosphate DNA backbone (Anderson et al., 1989). Consistent with this, a recent study demonstrated that Mu protein enhanced transfection efficiency by up to 11-fold wh ...
... that Mu helps condense DNA in the virus core by means of a charged-based interaction between the nine arginine residues in Mu and the phosphate DNA backbone (Anderson et al., 1989). Consistent with this, a recent study demonstrated that Mu protein enhanced transfection efficiency by up to 11-fold wh ...
Aluminum-Induced 133-ß-D-Glucan Inhibits Cell
... Symplastic intercellular transport in plants is achieved by plasmodesmata (PD). These cytoplasmic channels are well known to interconnect plant cells to facilitate intercellular movement of water, nutrients, and signaling molecules including hormones. However, it is not known whether Al may affect t ...
... Symplastic intercellular transport in plants is achieved by plasmodesmata (PD). These cytoplasmic channels are well known to interconnect plant cells to facilitate intercellular movement of water, nutrients, and signaling molecules including hormones. However, it is not known whether Al may affect t ...
Mitochondria and mammalian reproduction
... and Singh, 2007; Newmeyer and Ferguson-Miller, 2003; Scheffler, 2001). A few components of this machinery are encoded by resident mitochondrial DNA (mtDNA) a prokaryotic-like genome that is inherited maternally (Jansen and de Boer, 1998; St John et al., 2010). However, recent mitochondrial research f ...
... and Singh, 2007; Newmeyer and Ferguson-Miller, 2003; Scheffler, 2001). A few components of this machinery are encoded by resident mitochondrial DNA (mtDNA) a prokaryotic-like genome that is inherited maternally (Jansen and de Boer, 1998; St John et al., 2010). However, recent mitochondrial research f ...
Microscopy Microanalysis
... 14 days by optical microscopy for the presence of crystals within or around the colonies ~Bouquet et al., 1973!. Morphology and size characteristics of both crystals and microorganisms were analyzed by scanning electron microscopy ~SEM! and energy dispersive X-ray spectroscopy ~EDS!. Alternatively, ...
... 14 days by optical microscopy for the presence of crystals within or around the colonies ~Bouquet et al., 1973!. Morphology and size characteristics of both crystals and microorganisms were analyzed by scanning electron microscopy ~SEM! and energy dispersive X-ray spectroscopy ~EDS!. Alternatively, ...
Heading forwards: anterior visceral endoderm migration in
... region of three columnar Epi-VE cells. One AVE cell is outlined in green which relates to panel (c). The apical–basal polarity of the Epi-VE cells is shown via the coloured lines: purple, the basolateral domain; blue, the apical junctional domain; orange, the apicolateral domain. (b) En face surface ...
... region of three columnar Epi-VE cells. One AVE cell is outlined in green which relates to panel (c). The apical–basal polarity of the Epi-VE cells is shown via the coloured lines: purple, the basolateral domain; blue, the apical junctional domain; orange, the apicolateral domain. (b) En face surface ...
Document
... A: Something in cells can stimulate the GTPase activity B: Something in intro inhibits the GTPase activity The student and PI were mammalian biochemists. What system will they likely chose to identify the “something”? ...
... A: Something in cells can stimulate the GTPase activity B: Something in intro inhibits the GTPase activity The student and PI were mammalian biochemists. What system will they likely chose to identify the “something”? ...
Localization of lipids in freeze-dried mouse brain sections by
... The fact that the TOF-SIMS analysis is made in vacuum and that classical fixation schemes are often inappropriate to use since they alter the chemistry of the sample, means that sample preparation for TOF-SIMS analysis requires careful consideration. The two main strategies that have been used so f ...
... The fact that the TOF-SIMS analysis is made in vacuum and that classical fixation schemes are often inappropriate to use since they alter the chemistry of the sample, means that sample preparation for TOF-SIMS analysis requires careful consideration. The two main strategies that have been used so f ...
Structural and Functional Comparisons of Retroviral Envelope
... interesting model system by which to study the virological mechanisms of cancer, with potential applications for understanding human pulmonary carcinomas. Tumorigenesis of JSRV results from the transformation of differentiated lung epithelial cells in the alveoli and the bronchioli following infecti ...
... interesting model system by which to study the virological mechanisms of cancer, with potential applications for understanding human pulmonary carcinomas. Tumorigenesis of JSRV results from the transformation of differentiated lung epithelial cells in the alveoli and the bronchioli following infecti ...
Signal transduction
Signal transduction occurs when an extracellular signaling molecule activates a specific receptor located on the cell surface or inside the cell. In turn, this receptor triggers a biochemical chain of events inside the cell, creating a response. Depending on the cell, the response alters the cell's metabolism, shape, gene expression, or ability to divide. The signal can be amplified at any step. Thus, one signaling molecule can cause many responses.