Chromosomal DNA demethylation specified by
... Demethylation of the lacO sites is expected to occur when IPTG is withdrawn from the cultures, while the lacO sites should stay methylated in the cells that continue to receive IPTG. Cells from each of the six cell clones, meLacO2I3, meLacO2I12, meLacO2I19, meLacO22I8, meLacO22I16 and meLacO22I17, w ...
... Demethylation of the lacO sites is expected to occur when IPTG is withdrawn from the cultures, while the lacO sites should stay methylated in the cells that continue to receive IPTG. Cells from each of the six cell clones, meLacO2I3, meLacO2I12, meLacO2I19, meLacO22I8, meLacO22I16 and meLacO22I17, w ...
Di-(2-ethylhexyl) phthalate mediates glycolysis and the TCA cycle
... and then sharply decreased from 0.203 ± 0.022 μg g-1 to 0.104 ± 0.011 μg g-1 , and from 1.689 ± 0.018 μg g-1 to 1.172 ± 0.012 μg g-1, respectively. Comparative proteomic was conducted to investigate the global protein expression changes towards this contaminant exposure. Twenty-eight proteins with s ...
... and then sharply decreased from 0.203 ± 0.022 μg g-1 to 0.104 ± 0.011 μg g-1 , and from 1.689 ± 0.018 μg g-1 to 1.172 ± 0.012 μg g-1, respectively. Comparative proteomic was conducted to investigate the global protein expression changes towards this contaminant exposure. Twenty-eight proteins with s ...
cells
... “optical sectioning” of fluorescently-stained specimens. Only a single plane of focus is illuminated; out-of-focus fluorescence above and below the plane is subtracted by a computer. A sharp image results, as seen in stained nervous tissue (top), where nerve cells are green, support cells are red, a ...
... “optical sectioning” of fluorescently-stained specimens. Only a single plane of focus is illuminated; out-of-focus fluorescence above and below the plane is subtracted by a computer. A sharp image results, as seen in stained nervous tissue (top), where nerve cells are green, support cells are red, a ...
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... induce extensive proliferation, class switch recombination and somatic hypermutation. Epstein-‐Barr virus (EBV) can also induce B cell activation by mimicking T cell help through its main oncoprotein, latent membr ...
... induce extensive proliferation, class switch recombination and somatic hypermutation. Epstein-‐Barr virus (EBV) can also induce B cell activation by mimicking T cell help through its main oncoprotein, latent membr ...
Electrochemical detection of biological reactions using a novel nano
... suspensions were placed in the electrochemical cells. The bacterial response to phenol was measured on-line by applying a potential of 220 mV. The product of the enzymatic reaction, PAP, was monitored by its oxidation current. Additional measurements in the absence of the bacteria were performed to ...
... suspensions were placed in the electrochemical cells. The bacterial response to phenol was measured on-line by applying a potential of 220 mV. The product of the enzymatic reaction, PAP, was monitored by its oxidation current. Additional measurements in the absence of the bacteria were performed to ...
Receptores Monoaminérgicos en Corteza Prefrontal: Mecanismo de Acción de Fármacos Antipsicóticos
... antagonist phencyclidine (PCP) on PFC function to understand the cellular and network elements involved in its schizomimeitc actions. PCP induces a marked disruption of the activity of the PFC in the rat, increasing and decreasing the activity of 45% and 33% of the pyramidal neurons recorded, respec ...
... antagonist phencyclidine (PCP) on PFC function to understand the cellular and network elements involved in its schizomimeitc actions. PCP induces a marked disruption of the activity of the PFC in the rat, increasing and decreasing the activity of 45% and 33% of the pyramidal neurons recorded, respec ...
Poon, Andy: Predicting Phosphorylation: A critique of the NetPhos program and potential alternatives
... The likelihood of a given S/T/Y residue to be phosphorylated is defined by other sequences in the protein. Residues close to the S/T/Y in question may define this context, such that certain acidic or basic side chains are preferentially placed next to the phosphorylated residue in order to facilitat ...
... The likelihood of a given S/T/Y residue to be phosphorylated is defined by other sequences in the protein. Residues close to the S/T/Y in question may define this context, such that certain acidic or basic side chains are preferentially placed next to the phosphorylated residue in order to facilitat ...
Makorin ortholog LEP-2 regulates LIN-28 stability to
... It is increasingly clear from studies in several systems that lin-28 usually works with two other genes, let-7 and lin-41/Trim71, in a conserved ‘axis’ of negative interactions (Thornton and Gregory, 2012; Tsialikas and Romer-Seibert, 2015). Besides being involved in the J/A transition, this axis co ...
... It is increasingly clear from studies in several systems that lin-28 usually works with two other genes, let-7 and lin-41/Trim71, in a conserved ‘axis’ of negative interactions (Thornton and Gregory, 2012; Tsialikas and Romer-Seibert, 2015). Besides being involved in the J/A transition, this axis co ...
Enzymology of Plasma Membranes of Insect Intestinal Cells`
... tion or absorption (Cioffi, 1984); this specialization is observed even in the case of midguts with nominally only one cell type site of digestion and nutrient absorption in (Ferreira et al., 1981). The midgut is of detritivores, in other insects the hindgut is endodermal origin and the apical plasm ...
... tion or absorption (Cioffi, 1984); this specialization is observed even in the case of midguts with nominally only one cell type site of digestion and nutrient absorption in (Ferreira et al., 1981). The midgut is of detritivores, in other insects the hindgut is endodermal origin and the apical plasm ...
CELL MOTILITY: Spatial and Temporal Regulation of
... regions. Yet the coordinated effort of all of these similar molecules, in the context of their specific cell type, produces very distinct morphologies during each cell’s migration. The tremendous advances in molecular biology and biochemistry that have occurred in the past 20 years have allowed rese ...
... regions. Yet the coordinated effort of all of these similar molecules, in the context of their specific cell type, produces very distinct morphologies during each cell’s migration. The tremendous advances in molecular biology and biochemistry that have occurred in the past 20 years have allowed rese ...
Overview for Electrophoresis and Western Blotting
... glycine mobility is low enough to stack SDS-bound proteins ahead of the glycine zone even when the stacking gel is set up at the same pH as the resolving gel. Proteins as large as 70 kDa will remain stacked in a 4% gel at pH 8.6–8.8 just as well as in a 4% gel at 6.8–7.2. The larger proteins will al ...
... glycine mobility is low enough to stack SDS-bound proteins ahead of the glycine zone even when the stacking gel is set up at the same pH as the resolving gel. Proteins as large as 70 kDa will remain stacked in a 4% gel at pH 8.6–8.8 just as well as in a 4% gel at 6.8–7.2. The larger proteins will al ...
THE RDOA-DEPENDENT PHOSPHOPROTEOME PROFILE OF Salmonella enterica
... Chapter 4 Results ........................................................................................................................... 33 4.1 IHF is not an RdoA target .................................................................................................... 33 4.2 Comparison of ...
... Chapter 4 Results ........................................................................................................................... 33 4.1 IHF is not an RdoA target .................................................................................................... 33 4.2 Comparison of ...
REVIEWS
... possible in the adult mammalian brain. As the hippocampus had been implicated in human memory by the work of Penfield, and of Scoville and Milner, the finding of synaptic plasticity in this area of the brain was particularly intriguing54. Second, further investigation of this form of plasticity show ...
... possible in the adult mammalian brain. As the hippocampus had been implicated in human memory by the work of Penfield, and of Scoville and Milner, the finding of synaptic plasticity in this area of the brain was particularly intriguing54. Second, further investigation of this form of plasticity show ...
"Monoclonal Antibodies to Human Cell Surface Antigens". In
... which binds multiple ligands including iC3b and several of the ICAMS. The complex forms an adhesion molecule with signaling activity. Appears to be involved in inflammation. ...
... which binds multiple ligands including iC3b and several of the ICAMS. The complex forms an adhesion molecule with signaling activity. Appears to be involved in inflammation. ...
Structural genomics of proteins from conserved biochemical
... duplications followed by mutations to impart new substrate specificity could generate the three genes necessary to convert mevalonate into isopentenyl diphosphate. It appears more than fortuitous that these genes (and, in some cases, the gene encoding IDI2) are under the control of a single bacteria ...
... duplications followed by mutations to impart new substrate specificity could generate the three genes necessary to convert mevalonate into isopentenyl diphosphate. It appears more than fortuitous that these genes (and, in some cases, the gene encoding IDI2) are under the control of a single bacteria ...
PDF
... Elucidating the connections between genes and anatomy will require model morphogenetic processes that are amenable to detailed analysis of cell/tissue behaviors and to systems-level approaches to gene regulation. The formation of the calcified endoskeleton of the sea urchin embryo is a valuable expe ...
... Elucidating the connections between genes and anatomy will require model morphogenetic processes that are amenable to detailed analysis of cell/tissue behaviors and to systems-level approaches to gene regulation. The formation of the calcified endoskeleton of the sea urchin embryo is a valuable expe ...
What is new in tympanoplasty? - Romanian Journal of Rhinology
... The scaffold can give strength and mechanical properties similar to those of real tympanic membranes, while infills of biological materials (including growth factors as basic fibroblast growth factor bFGF) can encourage growth acceptance3. To study the mechanical properties of scaffolds, researchers ...
... The scaffold can give strength and mechanical properties similar to those of real tympanic membranes, while infills of biological materials (including growth factors as basic fibroblast growth factor bFGF) can encourage growth acceptance3. To study the mechanical properties of scaffolds, researchers ...
REVIEWS
... possible in the adult mammalian brain. As the hippocampus had been implicated in human memory by the work of Penfield, and of Scoville and Milner, the finding of synaptic plasticity in this area of the brain was particularly intriguing54. Second, further investigation of this form of plasticity show ...
... possible in the adult mammalian brain. As the hippocampus had been implicated in human memory by the work of Penfield, and of Scoville and Milner, the finding of synaptic plasticity in this area of the brain was particularly intriguing54. Second, further investigation of this form of plasticity show ...
Measurements of Single Molecules in Solution and Live Cells
... not just on observing single molecule signatures, but also on applying single-molecule detection to basic chemical and biological problems in applied and fundamental studies. Typically, single-molecule measurements, for example with a single enzyme molecule, are performed by absorption (immobilizati ...
... not just on observing single molecule signatures, but also on applying single-molecule detection to basic chemical and biological problems in applied and fundamental studies. Typically, single-molecule measurements, for example with a single enzyme molecule, are performed by absorption (immobilizati ...
Candidate genes and single nucleotide polymorphisms associated
... (Hubbard et al., 2009) were used as reference sequences. The SNP returned by the Maq SNPfilter command were annotated using a collection of command-line scripts (Grant et al., 2011) NGS-SNP (Grant et al., 2011) by assigning a functional class to each SNP (e.g., nonsynonymous) and then providing Ense ...
... (Hubbard et al., 2009) were used as reference sequences. The SNP returned by the Maq SNPfilter command were annotated using a collection of command-line scripts (Grant et al., 2011) NGS-SNP (Grant et al., 2011) by assigning a functional class to each SNP (e.g., nonsynonymous) and then providing Ense ...
Can`t live without them, can live with them: roles of caspases during
... while the apical caspase of the extrinsic apoptotic pathway caspase-8 is activated within the death-inducing signaling complex (DISC) [25–29]. An adaptor-independent oligomerization mechanism for activation of caspase-8 was also suggested recently [30]. On the other hand, activation of effector casp ...
... while the apical caspase of the extrinsic apoptotic pathway caspase-8 is activated within the death-inducing signaling complex (DISC) [25–29]. An adaptor-independent oligomerization mechanism for activation of caspase-8 was also suggested recently [30]. On the other hand, activation of effector casp ...
The Balance between Cell Division and Endoreplication Depends
... SCFSKP2A complex (del Pozo et al., 2002). The SCFSKP2A complex is an E3 ligase of ubiquitin that is composed of a common scaffold (CUL1, ASK, and RBX) (del Pozo and Estelle, 2000) and the F-box subunit SKP2A that specifically interacts with the targets. Furthermore, E2FC degradation was triggered du ...
... SCFSKP2A complex (del Pozo et al., 2002). The SCFSKP2A complex is an E3 ligase of ubiquitin that is composed of a common scaffold (CUL1, ASK, and RBX) (del Pozo and Estelle, 2000) and the F-box subunit SKP2A that specifically interacts with the targets. Furthermore, E2FC degradation was triggered du ...
Concerted Assembly and Cloning of Multiple DNA Segments Using
... endonuclease digestion, showed the predicted structures for each of four clones at each time point. Hence, both strategies provide high efficiency, rapid, and flexible approaches to simultaneous linking and cloning two DNA segments in an order-, orientation-, and reading frame-specific manner. We ob ...
... endonuclease digestion, showed the predicted structures for each of four clones at each time point. Hence, both strategies provide high efficiency, rapid, and flexible approaches to simultaneous linking and cloning two DNA segments in an order-, orientation-, and reading frame-specific manner. We ob ...
UVB induced cell cycle checkpoints in an early stage
... entrance into S phase imposed by DNA damage, the replication of damaged DNA can either result in cell death or an accumulation of genetic changes leading ultimately to cancer (Hartwell and kastan 1994). In mammalian cells, progression through the cell cycle is regulated by the ordered formation, act ...
... entrance into S phase imposed by DNA damage, the replication of damaged DNA can either result in cell death or an accumulation of genetic changes leading ultimately to cancer (Hartwell and kastan 1994). In mammalian cells, progression through the cell cycle is regulated by the ordered formation, act ...
Evolution of hard proteins in the sauropsid integument in relation to
... layer of flat cells that are incorporated into a variably thick corneous layer (depending on the type of scales) (Fig. 1I). In archosaurians (crocodilians and birds), the process of shedding of epidermal layers is not specifically known. However, the presence of a scission layer in avian scales (Can ...
... layer of flat cells that are incorporated into a variably thick corneous layer (depending on the type of scales) (Fig. 1I). In archosaurians (crocodilians and birds), the process of shedding of epidermal layers is not specifically known. However, the presence of a scission layer in avian scales (Can ...
Signal transduction
Signal transduction occurs when an extracellular signaling molecule activates a specific receptor located on the cell surface or inside the cell. In turn, this receptor triggers a biochemical chain of events inside the cell, creating a response. Depending on the cell, the response alters the cell's metabolism, shape, gene expression, or ability to divide. The signal can be amplified at any step. Thus, one signaling molecule can cause many responses.