Identification of Pseudomonas aeruginosa flagellin as - AJP-Lung
... ovary (CHO) cells transfected with Muc1 cDNA (CHO-Muc1) requires flagellin. CHO cells transfected with empty plasmid (CHO-X) or CHO-Muc1 cells were incubated with 35S-labeled P. aeruginosa wild-type (PAK/WT) or isogenic mutants devoid of pilin (PAK/NP), flagellin (PAK/fliC), or flagellar cap protein ...
... ovary (CHO) cells transfected with Muc1 cDNA (CHO-Muc1) requires flagellin. CHO cells transfected with empty plasmid (CHO-X) or CHO-Muc1 cells were incubated with 35S-labeled P. aeruginosa wild-type (PAK/WT) or isogenic mutants devoid of pilin (PAK/NP), flagellin (PAK/fliC), or flagellar cap protein ...
NIH Public Access - The Scripps Research Institute
... 4°C over 60 min, methionine biosynthetic inhibitory amino acids (25 mg/L each L-valine, Lisoleucine, L-leucine, L-lysine, L-threonine, L-pheynlalanine) and 90 mg/L of Lselenomethionine (Medicillin, catalog number MD045004D) were added. Gene expression was then induced by addition of isopropyl-β-D-th ...
... 4°C over 60 min, methionine biosynthetic inhibitory amino acids (25 mg/L each L-valine, Lisoleucine, L-leucine, L-lysine, L-threonine, L-pheynlalanine) and 90 mg/L of Lselenomethionine (Medicillin, catalog number MD045004D) were added. Gene expression was then induced by addition of isopropyl-β-D-th ...
Full Text - Labs / Projects - Fred Hutchinson Cancer Research Center
... DNA sequences, LUN (23) (Fig. 1D). Like human Topors, CG15104 interacted with Drosophila topoisomerase I and p53 (Dmp53) in vitro (Fig. 1A; see Fig. 3A). On the basis of this similarity and the conserved binding activities, we have named this Drosophila protein dTopors. The human and Drosophila Topo ...
... DNA sequences, LUN (23) (Fig. 1D). Like human Topors, CG15104 interacted with Drosophila topoisomerase I and p53 (Dmp53) in vitro (Fig. 1A; see Fig. 3A). On the basis of this similarity and the conserved binding activities, we have named this Drosophila protein dTopors. The human and Drosophila Topo ...
A painful TR(i)P to lysosomes
... of TRPA1-mediated Ca2+ responses: Complete removal of external Ca2+ failed to abolish AITC-induced [Ca2+]i increase and neuropeptide release in DRG neurons. Blocking TRPA1 channels with ruthenium red, a membrane-impermeable blocker, was also ineffective. However, the AITC-induced responses were enti ...
... of TRPA1-mediated Ca2+ responses: Complete removal of external Ca2+ failed to abolish AITC-induced [Ca2+]i increase and neuropeptide release in DRG neurons. Blocking TRPA1 channels with ruthenium red, a membrane-impermeable blocker, was also ineffective. However, the AITC-induced responses were enti ...
Direct Demonstration of a Physiological Role for Carbon Monoxide
... type 1 (HO-l) is induced by heme and is most abundantin the spleenand in other peripheraltissues,type 2 (HO-2) is the constitutive form which is expressedat high levels in the brain (Cruse and Maines, 1988; Maines, 1988; Sun et al., 1990). By in situ hybridization and immunohistochemistry,discrete n ...
... type 1 (HO-l) is induced by heme and is most abundantin the spleenand in other peripheraltissues,type 2 (HO-2) is the constitutive form which is expressedat high levels in the brain (Cruse and Maines, 1988; Maines, 1988; Sun et al., 1990). By in situ hybridization and immunohistochemistry,discrete n ...
Stress-Induced Chloroplast Degradation in
... characterized. The public microarray database (Winter et al., 2007) indicated that although At2g25625 expression was barely detectable in young tissues, its expression was greatly induced by abiotic stress and senescence during extensive chloroplast degradation (Hörtensteiner, 2006; Martínez et al., ...
... characterized. The public microarray database (Winter et al., 2007) indicated that although At2g25625 expression was barely detectable in young tissues, its expression was greatly induced by abiotic stress and senescence during extensive chloroplast degradation (Hörtensteiner, 2006; Martínez et al., ...
Tilting Plant Metabolism for Improved Metabolite Biosynthesis and
... the main trait of an innate immune system to cope with the hostile environment. Their metabolic plasticity evolves and exploits a range of inherent systems to create a rich repertoire of complex metabolites that hold adaptive significance for survival in diverse ecological niches. These phytochemica ...
... the main trait of an innate immune system to cope with the hostile environment. Their metabolic plasticity evolves and exploits a range of inherent systems to create a rich repertoire of complex metabolites that hold adaptive significance for survival in diverse ecological niches. These phytochemica ...
Spinalin, a new glycine- and histidine
... presence of unusual, small, collagen-like proteins in the wall (Kurz et al., 1991; Engel, 1997) and high-resolution electron microscopy has indicated that these are probably organized in collagen-like fibrils (Holstein et al., 1994). Nothing is known about the structure of the other parts of the nem ...
... presence of unusual, small, collagen-like proteins in the wall (Kurz et al., 1991; Engel, 1997) and high-resolution electron microscopy has indicated that these are probably organized in collagen-like fibrils (Holstein et al., 1994). Nothing is known about the structure of the other parts of the nem ...
A painful TR(i)P to lysosomes
... of TRPA1-mediated Ca2+ responses: Complete removal of external Ca2+ failed to abolish AITC-induced [Ca2+]i increase and neuropeptide release in DRG neurons. Blocking TRPA1 channels with ruthenium red, a membrane-impermeable blocker, was also ineffective. However, the AITC-induced responses were enti ...
... of TRPA1-mediated Ca2+ responses: Complete removal of external Ca2+ failed to abolish AITC-induced [Ca2+]i increase and neuropeptide release in DRG neurons. Blocking TRPA1 channels with ruthenium red, a membrane-impermeable blocker, was also ineffective. However, the AITC-induced responses were enti ...
GlischSpr11
... 16S rRNA gene: confirms that isolates belong to the genus Staphylococcus. Staphylococcus 16S rRNA codes for a protein that assists in translation. ...
... 16S rRNA gene: confirms that isolates belong to the genus Staphylococcus. Staphylococcus 16S rRNA codes for a protein that assists in translation. ...
A painful TR(i)P to lysosomes
... of TRPA1-mediated Ca2+ responses: Complete removal of external Ca2+ failed to abolish AITC-induced [Ca2+]i increase and neuropeptide release in DRG neurons. Blocking TRPA1 channels with ruthenium red, a membrane-impermeable blocker, was also ineffective. However, the AITC-induced responses were enti ...
... of TRPA1-mediated Ca2+ responses: Complete removal of external Ca2+ failed to abolish AITC-induced [Ca2+]i increase and neuropeptide release in DRG neurons. Blocking TRPA1 channels with ruthenium red, a membrane-impermeable blocker, was also ineffective. However, the AITC-induced responses were enti ...
A painful TR(i)P to lysosomes
... of TRPA1-mediated Ca2+ responses: Complete removal of external Ca2+ failed to abolish AITC-induced [Ca2+]i increase and neuropeptide release in DRG neurons. Blocking TRPA1 channels with ruthenium red, a membrane-impermeable blocker, was also ineffective. However, the AITC-induced responses were enti ...
... of TRPA1-mediated Ca2+ responses: Complete removal of external Ca2+ failed to abolish AITC-induced [Ca2+]i increase and neuropeptide release in DRG neurons. Blocking TRPA1 channels with ruthenium red, a membrane-impermeable blocker, was also ineffective. However, the AITC-induced responses were enti ...
Extracellular ATP Functions as an
... For example, extracellular ATP binds to P2X7 receptors to activate astrocyte Gln release, which modulates synaptic activity and participates in brain intercellular signaling (Duan et al., 2003). Although extracellular ATP released by intact plant tissues (Thomas et al., 2000) has no clearly defined ...
... For example, extracellular ATP binds to P2X7 receptors to activate astrocyte Gln release, which modulates synaptic activity and participates in brain intercellular signaling (Duan et al., 2003). Although extracellular ATP released by intact plant tissues (Thomas et al., 2000) has no clearly defined ...
Plant Cell
... characterized. The public microarray database (Winter et al., 2007) indicated that although At2g25625 expression was barely detectable in young tissues, its expression was greatly induced by abiotic stress and senescence during extensive chloroplast degradation (Hörtensteiner, 2006; Martínez et al., ...
... characterized. The public microarray database (Winter et al., 2007) indicated that although At2g25625 expression was barely detectable in young tissues, its expression was greatly induced by abiotic stress and senescence during extensive chloroplast degradation (Hörtensteiner, 2006; Martínez et al., ...
Polypeptide Composition of Envelopes of Spinach Chloroplasts
... and Benz 1984). The inner envelope membranes act as a substantial barrier for both low and high molecular mass compounds. The membranes are sites of ion and metabolite translocation and synthesis of galactolipids (Douce and Joyard 1990, Flugge and Heldt 1991). The envelope membranes were first prepa ...
... and Benz 1984). The inner envelope membranes act as a substantial barrier for both low and high molecular mass compounds. The membranes are sites of ion and metabolite translocation and synthesis of galactolipids (Douce and Joyard 1990, Flugge and Heldt 1991). The envelope membranes were first prepa ...
The Stress-Induced Atf3-Gelsolin Cascade Underlies
... cell-autonomous program consisting of a stress-induced Atf3-gelsolin cascade affects the change in dendritic spine morphology following mTOR hyperactivation. This previously unidentified molecular cascade could be a therapeutic target for treating mTORopathies. Key words: autism; dendiritic spines; ...
... cell-autonomous program consisting of a stress-induced Atf3-gelsolin cascade affects the change in dendritic spine morphology following mTOR hyperactivation. This previously unidentified molecular cascade could be a therapeutic target for treating mTORopathies. Key words: autism; dendiritic spines; ...
effect of ion concentration changes in t
... cells related to morphology of t-tubules, and to the particular properties of ion transfer mechanisms. To increase the accuracy, the present model was upgraded by incorporation of a description of ion diffusion within t-tubular lumen in radial direction. The simulations suggest that, depending on the ...
... cells related to morphology of t-tubules, and to the particular properties of ion transfer mechanisms. To increase the accuracy, the present model was upgraded by incorporation of a description of ion diffusion within t-tubular lumen in radial direction. The simulations suggest that, depending on the ...
Assisted morphogenesis: glial control of dendrite
... domains are also present in a-tectorin, a major component of the tectorial membrane, a highly organized proteinaceous ECM that anchors the ciliated outer hair cells of the inner ear [35]. Mutations in dyf-7 and dex-1 exhibit genetic interactions suggestive of physical binding. Furthermore, polymeriz ...
... domains are also present in a-tectorin, a major component of the tectorial membrane, a highly organized proteinaceous ECM that anchors the ciliated outer hair cells of the inner ear [35]. Mutations in dyf-7 and dex-1 exhibit genetic interactions suggestive of physical binding. Furthermore, polymeriz ...
Electrical Interactions via the Extracellular Potential Near Cell Bodies
... Figure 3. Field potentials in a plane around the simulated layer V cortical pyramidal cell. x and y-axes are in units of µm. Each trace is the potential as a function of time at a point located at the center of the trace. Note the different voltage scales indicated by the different colors of traces. ...
... Figure 3. Field potentials in a plane around the simulated layer V cortical pyramidal cell. x and y-axes are in units of µm. Each trace is the potential as a function of time at a point located at the center of the trace. Note the different voltage scales indicated by the different colors of traces. ...
Chapter 7 Body Systems
... Neurotransmitters—means by which neurons communicate with one another; there are more than 30 compounds known to be neurotransmitters, and dozens of others are suspected ...
... Neurotransmitters—means by which neurons communicate with one another; there are more than 30 compounds known to be neurotransmitters, and dozens of others are suspected ...
Structural Prediction of Membrane
... cal characteristics (e.g. hydrophobicity) for each amino acid known soluble protein structures which again are unlikely within the sequence. A plot of the additive function versus to reproduce the membrane environment given their interior sequence number results in maxima and minima indicating hydro ...
... cal characteristics (e.g. hydrophobicity) for each amino acid known soluble protein structures which again are unlikely within the sequence. A plot of the additive function versus to reproduce the membrane environment given their interior sequence number results in maxima and minima indicating hydro ...
SOMBRERO, BEARSKIN1, and BEARSKIN2 Regulate Root Cap
... altering cell wall pH (Wen et al., 1999). In Arabidopsis, two endob-1,4-D-glucanases (cellulases), which probably hydrolyze the intramolecular bonds in cellulose molecules, have also been implicated in the detachment of the root cap (del Campillo et al., 2004). Arabidopsis, along with other Brassica ...
... altering cell wall pH (Wen et al., 1999). In Arabidopsis, two endob-1,4-D-glucanases (cellulases), which probably hydrolyze the intramolecular bonds in cellulose molecules, have also been implicated in the detachment of the root cap (del Campillo et al., 2004). Arabidopsis, along with other Brassica ...
Plant Chloroplasts and Other Plastids
... amino acids are actively loaded into the sieve element/ companion cell complex of the vascular tissue by specific H 1 symporters. The TPT was the first plant membrane transport system for which the primary sequence was determined. Analyses of TPT sequences from various plants showed that all TPTs are ...
... amino acids are actively loaded into the sieve element/ companion cell complex of the vascular tissue by specific H 1 symporters. The TPT was the first plant membrane transport system for which the primary sequence was determined. Analyses of TPT sequences from various plants showed that all TPTs are ...
Autophagy and cell death in model organisms
... programmed cell death (or apoptosis) is characterized by dependence on caspases, chromatin condensation and fragmentation and overall cell shrinkage. Blebbing of the plasma membrane leads to the formation of apoptotic bodies, which are ingested by phagocytes. Type 2 (or autophagic cell death) is cha ...
... programmed cell death (or apoptosis) is characterized by dependence on caspases, chromatin condensation and fragmentation and overall cell shrinkage. Blebbing of the plasma membrane leads to the formation of apoptotic bodies, which are ingested by phagocytes. Type 2 (or autophagic cell death) is cha ...
Asymmetric Cell Division as a Route to Reduction in Cell Length
... microtubules occurs at the point at which the flagellum emerges from the cell body (Hemphill et al. 1991). Individual microtubules within the subpellicular array are stable with little turnover and the cytoskeleton is remarkably constant throughout the cell division cycle (Sherwin and Gull 1989a, 19 ...
... microtubules occurs at the point at which the flagellum emerges from the cell body (Hemphill et al. 1991). Individual microtubules within the subpellicular array are stable with little turnover and the cytoskeleton is remarkably constant throughout the cell division cycle (Sherwin and Gull 1989a, 19 ...
Signal transduction
Signal transduction occurs when an extracellular signaling molecule activates a specific receptor located on the cell surface or inside the cell. In turn, this receptor triggers a biochemical chain of events inside the cell, creating a response. Depending on the cell, the response alters the cell's metabolism, shape, gene expression, or ability to divide. The signal can be amplified at any step. Thus, one signaling molecule can cause many responses.