Electrical Properties of the Pacemaker Neurons in the Heart
... since on stimulation of them acceleratory or inhibitory effects are observed in the heartbeat. Irisawa and Hama (1965) found, with the aid of the electron microscope, that the nerve fibers in this ganglion were often tied close together, separated only by a very thin double plasma membrane (apparent ...
... since on stimulation of them acceleratory or inhibitory effects are observed in the heartbeat. Irisawa and Hama (1965) found, with the aid of the electron microscope, that the nerve fibers in this ganglion were often tied close together, separated only by a very thin double plasma membrane (apparent ...
guideline on development, production, characterisation
... As for any medicinal product, the manufacturing process of a product that has been developed using a platform manufacturing approach should be appropriately validated at the time of marketing authorisation application. Validation studies should include data derived from the final manufacturing proce ...
... As for any medicinal product, the manufacturing process of a product that has been developed using a platform manufacturing approach should be appropriately validated at the time of marketing authorisation application. Validation studies should include data derived from the final manufacturing proce ...
NIH Public Access
... calculated CI scores using CompuSyn (38), and found that these drugs were synergistic in both cell lines (average CI scores were 0.34±0.19 for DDLS8817 and 0.63±0.17 for LPS141) (Fig. 2). In addition, the IGF1R small molecule inhibitor NVP-AEW541 appeared to be synergistic in combination with PD0332 ...
... calculated CI scores using CompuSyn (38), and found that these drugs were synergistic in both cell lines (average CI scores were 0.34±0.19 for DDLS8817 and 0.63±0.17 for LPS141) (Fig. 2). In addition, the IGF1R small molecule inhibitor NVP-AEW541 appeared to be synergistic in combination with PD0332 ...
Treadmilling by FtsZ filaments drives peptidoglycan
... synthases and other proteins involved in cytokinesis (4, 5). Once the division machinery is mature, the cytosolic Z ring constricts, while the associated enzymes on the other side of the membrane build a PG-containing septum that partitions the cell in two. Whereas a detailed catalog of cell divisio ...
... synthases and other proteins involved in cytokinesis (4, 5). Once the division machinery is mature, the cytosolic Z ring constricts, while the associated enzymes on the other side of the membrane build a PG-containing septum that partitions the cell in two. Whereas a detailed catalog of cell divisio ...
Fingolimod attenuates experimental autoimmune neuritis and
... (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. ...
... (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. ...
by Bone Marrow Mesenchymal Stem Cell Secretions 2011
... Bone marrow mesenchymal stem cells (MSCs) may soon become the first FDA-approved stem cell therapy for autoimmune and inflammatory disease. Our lab originally hypothesized that much of the therapeutic activity of MSCs may be attributed to molecules secreted by these cells. This thesis will test this ...
... Bone marrow mesenchymal stem cells (MSCs) may soon become the first FDA-approved stem cell therapy for autoimmune and inflammatory disease. Our lab originally hypothesized that much of the therapeutic activity of MSCs may be attributed to molecules secreted by these cells. This thesis will test this ...
Biofilm exopolysaccharides
... immobilization of cells and enzymes. This is not seen in bacterial alginates from Azotobacter vinelandii, even though these EPS closely resemble the algal alginates in possessing sequences of polyguluronic acid blocks producing the characteristic egg-box structure (Ertesvag & Valla, 1998). The bacte ...
... immobilization of cells and enzymes. This is not seen in bacterial alginates from Azotobacter vinelandii, even though these EPS closely resemble the algal alginates in possessing sequences of polyguluronic acid blocks producing the characteristic egg-box structure (Ertesvag & Valla, 1998). The bacte ...
Inorganic pyrophosphate is located primarily in the mitochondria of
... (i.e. about 5 gM) if the enzyme UTP-glucose-1-phosphate uridylyltransferase (EC 2.7.7.9) is assumed to be in equilibrium in the cytosol (Guynn et al., 1974; Veech et al., 1980). This low value would also imply a mitochondrial location for most of the PP1. In this paper we have extended our previous ...
... (i.e. about 5 gM) if the enzyme UTP-glucose-1-phosphate uridylyltransferase (EC 2.7.7.9) is assumed to be in equilibrium in the cytosol (Guynn et al., 1974; Veech et al., 1980). This low value would also imply a mitochondrial location for most of the PP1. In this paper we have extended our previous ...
BH3 mimetics: Their action and efficacy in
... of ABT-737, has been shown to be significantly effective in most CLL patients in clinical trials, and ABT-199 (venetoclax), also has shown to be effective in patients with relapsed or refractory CLL [13,30]. Other BH3 mimetics, GX15-070 (obatoclax), Bl-97C1 (sabutoclax), AT-101 (gossypol) and deriva ...
... of ABT-737, has been shown to be significantly effective in most CLL patients in clinical trials, and ABT-199 (venetoclax), also has shown to be effective in patients with relapsed or refractory CLL [13,30]. Other BH3 mimetics, GX15-070 (obatoclax), Bl-97C1 (sabutoclax), AT-101 (gossypol) and deriva ...
On the Morphology of the Drosophila Heart
... provided new insights into general aspects of cardiogenesis, heart physiology and cardiac aging, to name a few examples. In recent years, the Drosophila heart has also attracted the attention of researchers in the field of biomedicine. This development is mainly due to the fact that several genes ca ...
... provided new insights into general aspects of cardiogenesis, heart physiology and cardiac aging, to name a few examples. In recent years, the Drosophila heart has also attracted the attention of researchers in the field of biomedicine. This development is mainly due to the fact that several genes ca ...
Sucrose Cleavage Pathways in Aspen Wood
... et al., 2006). In another example, co-immuno-detection of Arabidopsis SUS2 and E37 which is a polypeptide of the inner plastid envelope showed SUS association with plastids of Arabidopsis thaliana embryo, endosperm and seed coat (Nunez et al., 2008). In addition, immuno-localization studies in which ...
... et al., 2006). In another example, co-immuno-detection of Arabidopsis SUS2 and E37 which is a polypeptide of the inner plastid envelope showed SUS association with plastids of Arabidopsis thaliana embryo, endosperm and seed coat (Nunez et al., 2008). In addition, immuno-localization studies in which ...
machinery pre-mRNA in sensing defects in the spliceosomal Mdm4
... (ER) mice, which allowed the triggering of a recombination event in both live animals and, ex vivo, primary cells by using 4-hydroxytamoxifen (OHT). We switched to the Prmt5F/FER system for three main reasons: First, it allowed us to look at cell-autonomous defects. Second, we could derive a much la ...
... (ER) mice, which allowed the triggering of a recombination event in both live animals and, ex vivo, primary cells by using 4-hydroxytamoxifen (OHT). We switched to the Prmt5F/FER system for three main reasons: First, it allowed us to look at cell-autonomous defects. Second, we could derive a much la ...
Scrapie infected tissues
... In tissues obtained from ME7 infected mice at 70 dpi and at terminal disease, TBM’s showed marked immunogold labelling for PrPd within lysosomes. In some of these lysosomes, immunogold deposits were concentrated at one particular pole, associated with a more electron dense floccular material. A mino ...
... In tissues obtained from ME7 infected mice at 70 dpi and at terminal disease, TBM’s showed marked immunogold labelling for PrPd within lysosomes. In some of these lysosomes, immunogold deposits were concentrated at one particular pole, associated with a more electron dense floccular material. A mino ...
Cholinergic Responses and Intrinsic Membrane Properties of
... Off-line analyses were performed using Clampfit 10 software (Molecular Devices). Only cells with action potential amplitude higher than 50 mV and stable resting membrane potential (if not spontaneously firing, more negative than ⫺45 mV) were included in the statistical analysis. The following intrin ...
... Off-line analyses were performed using Clampfit 10 software (Molecular Devices). Only cells with action potential amplitude higher than 50 mV and stable resting membrane potential (if not spontaneously firing, more negative than ⫺45 mV) were included in the statistical analysis. The following intrin ...
Standard PDF - Wiley Online Library
... photon energy. The inhomogeneity inherent to most biological specimen, and in particular, to the internal structure of various cells, leads to high quality SHG images without any preconditioning such as labelling or staining that might induce undesirable effects in the living cell (Reshak, 2009). Hi ...
... photon energy. The inhomogeneity inherent to most biological specimen, and in particular, to the internal structure of various cells, leads to high quality SHG images without any preconditioning such as labelling or staining that might induce undesirable effects in the living cell (Reshak, 2009). Hi ...
LPS- or Pseudomonas aeruginosa- mediated activation of
... line RAW264.7) with polyunsaturated fatty acids (PUFA) for 72 h in a concentration of 15 µM resulted in an incorporation of these fatty acids into both non-raft and lipid raft membrane domains (Schumann et al., 2011). This PUFA integration altered the physicalchemical properties of the membrane micr ...
... line RAW264.7) with polyunsaturated fatty acids (PUFA) for 72 h in a concentration of 15 µM resulted in an incorporation of these fatty acids into both non-raft and lipid raft membrane domains (Schumann et al., 2011). This PUFA integration altered the physicalchemical properties of the membrane micr ...
Induction of a Secondary Body Axis in Xenopus by Antibodies to
... embryos causes the formation of a secondary body axis, while their injection into embryos ventralized by UV irradiation partially rescues the primary dorsoanterior axis. The evidence indicates that this effect of the Fabs is specific. Neither the process of microinjection nor the introduction of thi ...
... embryos causes the formation of a secondary body axis, while their injection into embryos ventralized by UV irradiation partially rescues the primary dorsoanterior axis. The evidence indicates that this effect of the Fabs is specific. Neither the process of microinjection nor the introduction of thi ...
Lateral relocation of auxin efflux regulator PIN3 mediates tropism in
... letters to nature positioning (Fig. 5f). A similar pattern of PIN3 localization was observed when the actin cytoskeleton was disrupted by latrunculin B (Fig. 5g) or cytochalasin D (data not shown) treatment, suggesting an actin-dependent cycling of PIN3. A similar cycling pattern has been demonstra ...
... letters to nature positioning (Fig. 5f). A similar pattern of PIN3 localization was observed when the actin cytoskeleton was disrupted by latrunculin B (Fig. 5g) or cytochalasin D (data not shown) treatment, suggesting an actin-dependent cycling of PIN3. A similar cycling pattern has been demonstra ...
Development of an enzyme-linked immunosorbent assay and a beta
... 1M MES, pH 3.5, and 200 ml ovalbumin (OV) in DDW (2 mg in DDW; molar ratio of 10:1 ATL-acid:OV), which were added by slow pipetting. The reaction was allowed to proceed for 2 h at room temperature, and the unbound hapten and other small-molecular-weight components were separated from the protein-hap ...
... 1M MES, pH 3.5, and 200 ml ovalbumin (OV) in DDW (2 mg in DDW; molar ratio of 10:1 ATL-acid:OV), which were added by slow pipetting. The reaction was allowed to proceed for 2 h at room temperature, and the unbound hapten and other small-molecular-weight components were separated from the protein-hap ...
Intracellular calcium: friend or foe?
... outside, the cell is able to accumulate up to millimolar concentrations of the indicator inside. Though these fluors have now been used widely in many cells types their precise intracellular location has rarely been fully documented. Other complications can be ester hydrolysis, occurring extracellul ...
... outside, the cell is able to accumulate up to millimolar concentrations of the indicator inside. Though these fluors have now been used widely in many cells types their precise intracellular location has rarely been fully documented. Other complications can be ester hydrolysis, occurring extracellul ...
FasL gene knock-down therapy enhances the
... brain cancer in the central nervous system. Currently, the mean survival of patients affected by glioblastoma multiforme (GBM; the most aggressive form of malignant glioma) after conventional therapy consisting of surgical resection followed by radiotherapy and chemotherapy, remains just over 14 mon ...
... brain cancer in the central nervous system. Currently, the mean survival of patients affected by glioblastoma multiforme (GBM; the most aggressive form of malignant glioma) after conventional therapy consisting of surgical resection followed by radiotherapy and chemotherapy, remains just over 14 mon ...
Genetic Control of Fusion Pore Expansion in the Epidermis of
... encoding type I membrane proteins required for diverse epithelial cell fusion reactions. Mutations in eff-1 result in failure of epithelial cell fusion and developmental defects in organs where cell fusion normally occurs (Mohler et al., 2002; Shemer, 2002; Shemer and Podbilewicz, 2002). The activit ...
... encoding type I membrane proteins required for diverse epithelial cell fusion reactions. Mutations in eff-1 result in failure of epithelial cell fusion and developmental defects in organs where cell fusion normally occurs (Mohler et al., 2002; Shemer, 2002; Shemer and Podbilewicz, 2002). The activit ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.