Xyloglucan and its Interactions with Other Components of the
... of four glucose residues in the backbone with three xylose and a smaller number of galactose and fucose substituents. These oligosaccharides are readily identified by high-performance anion exchange chromatography with pulsed amperometric detection or matrix-assisted laser-desorption/ionization time ...
... of four glucose residues in the backbone with three xylose and a smaller number of galactose and fucose substituents. These oligosaccharides are readily identified by high-performance anion exchange chromatography with pulsed amperometric detection or matrix-assisted laser-desorption/ionization time ...
Theme 1 Molecules, Cells and the Basis for Disease
... 20.1 Understanding conformational dynamics and allosteric modulation in immunoglobulin E ............ 23 22.1 Mechanotransduction in Cardiac Myocytes ...................................................................................... 24 23.1 Molecular regulation of collective cell migrati ...
... 20.1 Understanding conformational dynamics and allosteric modulation in immunoglobulin E ............ 23 22.1 Mechanotransduction in Cardiac Myocytes ...................................................................................... 24 23.1 Molecular regulation of collective cell migrati ...
hematopoietic stem cells: to be or notch to be
... The hematopoietic system has served for decades as a pioneer model for studying and deciphering the behavior of somatic Stem Cells and the mechanisms that regulate cell differentiation, and in fact, the knowledge generated on hematopoietic stem cell biology has guided the understanding of other type ...
... The hematopoietic system has served for decades as a pioneer model for studying and deciphering the behavior of somatic Stem Cells and the mechanisms that regulate cell differentiation, and in fact, the knowledge generated on hematopoietic stem cell biology has guided the understanding of other type ...
Lysosomes in iron metabolism, ageing and apoptosis
... proteins (see ‘‘Lipofuscin and postmitotic ageing’’). It was soon recognized that many previously known storage diseases, often with a serious outcome, are due to mutated and inactive lysosomal hydrolases. As a consequence, substrates cannot be degraded by these faulty enzymes, but rather accumulate ...
... proteins (see ‘‘Lipofuscin and postmitotic ageing’’). It was soon recognized that many previously known storage diseases, often with a serious outcome, are due to mutated and inactive lysosomal hydrolases. As a consequence, substrates cannot be degraded by these faulty enzymes, but rather accumulate ...
Human microcephaly ASPM protein is a spindle pole
... when the activity of kinesin-14 or a centrosomal MT nucleation factor is suppressed; normally, kinesin-14 and CDK5RAP2 are active enough to mask the function of ASPM in this cell type. This scenario may hold true for many other cell types in mammals. In a mouse model, mutations in Aspm cause a reduc ...
... when the activity of kinesin-14 or a centrosomal MT nucleation factor is suppressed; normally, kinesin-14 and CDK5RAP2 are active enough to mask the function of ASPM in this cell type. This scenario may hold true for many other cell types in mammals. In a mouse model, mutations in Aspm cause a reduc ...
Redistribution of membrane proteins between the Golgi apparatus
... and movement of the ER network (Boevink et al., 1998; Liebe and Quader, 1994; Quader, 1990). Application of ¯uorescent protein technology has started to revolutionise plant cell biology and the study of the secretory pathway is no exception. It is now possible to observe in vivo the dynamic events o ...
... and movement of the ER network (Boevink et al., 1998; Liebe and Quader, 1994; Quader, 1990). Application of ¯uorescent protein technology has started to revolutionise plant cell biology and the study of the secretory pathway is no exception. It is now possible to observe in vivo the dynamic events o ...
during Epstein-Barr Virus Reactivation Transcription by Lytic
... This work was supported by National Natural Science Foundation of China (Grant 30771981), National Basic Research Program of China (973 Program, Grant 2006CB504305), and National High-Tech Research and Development Plan (863 Program, Grant 2006AA02A245). ...
... This work was supported by National Natural Science Foundation of China (Grant 30771981), National Basic Research Program of China (973 Program, Grant 2006CB504305), and National High-Tech Research and Development Plan (863 Program, Grant 2006AA02A245). ...
Cells Double-Stranded RNA in Myeloid Dendritic DHX9 Pairs with
... gene expression through its binding to TAR RNA, indicating that DHX9 is also an RNA binding protein (17). We decided to investigate the function of DHX9 in sensing dsRNA and RNA virus. We therefore established stable D2SC cell lines expressing shRNA to knockdown expression of DHX9, MyD88, IPS-1, RIG ...
... gene expression through its binding to TAR RNA, indicating that DHX9 is also an RNA binding protein (17). We decided to investigate the function of DHX9 in sensing dsRNA and RNA virus. We therefore established stable D2SC cell lines expressing shRNA to knockdown expression of DHX9, MyD88, IPS-1, RIG ...
The journey of developing hematopoietic stem cells
... permanently reconstitute the hematopoietic system of the recipient have not been successful, although differentiated hematopoietic cells can be generated with relative ease (Keller, 2005; Kyba and Daley, 2003; Wang et al., 2005a). So far, robust and sustained multi-lineage reconstitution of adult he ...
... permanently reconstitute the hematopoietic system of the recipient have not been successful, although differentiated hematopoietic cells can be generated with relative ease (Keller, 2005; Kyba and Daley, 2003; Wang et al., 2005a). So far, robust and sustained multi-lineage reconstitution of adult he ...
Churchill, a Zinc Finger Transcriptional Activator, Regulates the
... does not include a nuclear localization signal, and since the protein does not localize to the nucleus until midto-late blastula, it is likely that ChCh is carried to the nucleus by another protein that appears at this time. What is the function of ChCh? As an initial approach, we injected ChCh mRNA ...
... does not include a nuclear localization signal, and since the protein does not localize to the nucleus until midto-late blastula, it is likely that ChCh is carried to the nucleus by another protein that appears at this time. What is the function of ChCh? As an initial approach, we injected ChCh mRNA ...
Cultivation of Trypanosoma brucei sspp. in
... folic acid which were dissolved in small volumes of O - ~ N - N ~ OMedia sterilized by filtration (0.2 pm cellulose nitrate filters) and were stored at - 15 . Culture vessels were washed with detergent 7 X (Linbro Chemical Co. Inc., Newhaven, Connecticut, U.S.A.) or, when necessary, with chromic or ...
... folic acid which were dissolved in small volumes of O - ~ N - N ~ OMedia sterilized by filtration (0.2 pm cellulose nitrate filters) and were stored at - 15 . Culture vessels were washed with detergent 7 X (Linbro Chemical Co. Inc., Newhaven, Connecticut, U.S.A.) or, when necessary, with chromic or ...
Differential Effects of Rapamycin on Mammalian Target of
... day until colonies were visible to the naked eye. Colonies were stained with plating 50,000 cells/ml in the presence or absence of 20 nM rapamycin and crystal violet (0.1% in 20% methanol). FL5.12 cells were maintained in RPMI measuring cell number at 24-h intervals with a Coulter Z2 particle analyz ...
... day until colonies were visible to the naked eye. Colonies were stained with plating 50,000 cells/ml in the presence or absence of 20 nM rapamycin and crystal violet (0.1% in 20% methanol). FL5.12 cells were maintained in RPMI measuring cell number at 24-h intervals with a Coulter Z2 particle analyz ...
alters cell wall construction
... binding domain (ABD) of the Arabidopsis fimbrin AtFIM1 fused to GFP (FABD2-GFP) were used. FABD2-GFP transgenic Arabidopsis seedlings appear to have no phenotypic differences from the wild type and have been widely used to analyze actin cytoskeletal organization in Arabidopsis lines (Voigt et al., 20 ...
... binding domain (ABD) of the Arabidopsis fimbrin AtFIM1 fused to GFP (FABD2-GFP) were used. FABD2-GFP transgenic Arabidopsis seedlings appear to have no phenotypic differences from the wild type and have been widely used to analyze actin cytoskeletal organization in Arabidopsis lines (Voigt et al., 20 ...
GLYCOLIPID ANCHORING OF CELL SURFACE PROTEINS Mark C
... property was lost on PI-PLC treatment suggesting that the lipophilic properties of AP were intimately associated with AP itself, rather than being some property of the lipid bilayer with which it was associated, and could be explained by the covalent linkage of a PI molecule to the protein. Similar ...
... property was lost on PI-PLC treatment suggesting that the lipophilic properties of AP were intimately associated with AP itself, rather than being some property of the lipid bilayer with which it was associated, and could be explained by the covalent linkage of a PI molecule to the protein. Similar ...
Aubé et al., J Immunol, 2014
... spectral overlap. Of note, laser power was kept to a minimum to avoid photodamage, that is, ∼5–10 mW at the sample. Imaging was performed with an Olympus Ultra 325 MPE water immersion objective (1.05 numerical aperture, working distance of 2 mm) at a 512 3 512 resolution, using a pixel dwell time of ...
... spectral overlap. Of note, laser power was kept to a minimum to avoid photodamage, that is, ∼5–10 mW at the sample. Imaging was performed with an Olympus Ultra 325 MPE water immersion objective (1.05 numerical aperture, working distance of 2 mm) at a 512 3 512 resolution, using a pixel dwell time of ...
Positive and Negative Regulation of Muscle Cell
... fully differentiated as slow muscle fibers (data not shown). Presumably, these extra slow muscle cells are formed at the expense of fast muscle because they occupy the locations where fast muscle cells normally form, and because nearly all the muscle cells in the somite exhibited these slow muscle p ...
... fully differentiated as slow muscle fibers (data not shown). Presumably, these extra slow muscle cells are formed at the expense of fast muscle because they occupy the locations where fast muscle cells normally form, and because nearly all the muscle cells in the somite exhibited these slow muscle p ...
Neuromesodermal progenitors and the making of the
... then decreases during the generation of the body axis. Retrospective clonal analysis does not directly indicate the location of NMps in the embryo. However, fate-mapping studies in which small groups of cells were labelled have helped to identify regions where NMps may reside in the embryo. In the c ...
... then decreases during the generation of the body axis. Retrospective clonal analysis does not directly indicate the location of NMps in the embryo. However, fate-mapping studies in which small groups of cells were labelled have helped to identify regions where NMps may reside in the embryo. In the c ...
Transcriptionally repressed germ cells lack a
... ognized by mAb H5 is present in somatic nuclei, but not in was detected in germ-line blastomeres from the 1-cell stage to germ-line nuclei, in pregastrulation C. elegans and Drosophila the 100-cell stage (Fig. 4, second column). In contrast, mAb embryos. H5 staining was restricted to somatic blastom ...
... ognized by mAb H5 is present in somatic nuclei, but not in was detected in germ-line blastomeres from the 1-cell stage to germ-line nuclei, in pregastrulation C. elegans and Drosophila the 100-cell stage (Fig. 4, second column). In contrast, mAb embryos. H5 staining was restricted to somatic blastom ...
Transcriptionally repressed germ cells lack a subpopulation of
... ognized by mAb H5 is present in somatic nuclei, but not in was detected in germ-line blastomeres from the 1-cell stage to germ-line nuclei, in pregastrulation C. elegans and Drosophila the 100-cell stage (Fig. 4, second column). In contrast, mAb embryos. H5 staining was restricted to somatic blastom ...
... ognized by mAb H5 is present in somatic nuclei, but not in was detected in germ-line blastomeres from the 1-cell stage to germ-line nuclei, in pregastrulation C. elegans and Drosophila the 100-cell stage (Fig. 4, second column). In contrast, mAb embryos. H5 staining was restricted to somatic blastom ...
Study The Effects Of Methotrexate (MTX) On Some Parameters Of
... reproduction of the cancer cells. Methotrexate is used in much smaller doses for the treatment of rheumatoid arthritis, Crohn’s disease and psoriasis [1]. Methotrexate is known to be teratogenic in women, but few data are available on the effects of methotrexate on male reproductive capability. Stud ...
... reproduction of the cancer cells. Methotrexate is used in much smaller doses for the treatment of rheumatoid arthritis, Crohn’s disease and psoriasis [1]. Methotrexate is known to be teratogenic in women, but few data are available on the effects of methotrexate on male reproductive capability. Stud ...
Mona Sayed Ahmed Mohammed Ellithey S12000541
... with a selectivity index of SI 8.1 which was confirmed by real time cell electronic sensing (RT-CES). Compounds 2, 7 and 8 showed strong inhibitory activity against HIV-1 PR at IC50‘s of 7.2 ±0.7, 4.85±0.2 and 4.80 ±0.9 µM respectively. Docking studies gave comparable scores when comparing the bindi ...
... with a selectivity index of SI 8.1 which was confirmed by real time cell electronic sensing (RT-CES). Compounds 2, 7 and 8 showed strong inhibitory activity against HIV-1 PR at IC50‘s of 7.2 ±0.7, 4.85±0.2 and 4.80 ±0.9 µM respectively. Docking studies gave comparable scores when comparing the bindi ...
THE ROLE OF FIBROBLAST GROWTH FACTOR (FGF) AND TYPE
... target cells for its activity 9. In 1990, Joyce, et al 50 showed that the effect of TGF-(3 on the periosteum is first to stimulate the proliferation of mesenchymal cells that then differentiate into chondrocites and osteoblast. The net result of coninued TGF(3 injection was the formation of a callus ...
... target cells for its activity 9. In 1990, Joyce, et al 50 showed that the effect of TGF-(3 on the periosteum is first to stimulate the proliferation of mesenchymal cells that then differentiate into chondrocites and osteoblast. The net result of coninued TGF(3 injection was the formation of a callus ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.