The Sad1-UNC-84 homology domain in Mps3 interacts with Mps2 to
... three primary layers called the outer, inner, and central plaques. Cytoplasmic and nuclear microtubules are nucleated from the outer and inner plaques, respectively, whereas the central layer of the SPB plays an important role in tethering the organelle to the nuclear envelope. Associated with one s ...
... three primary layers called the outer, inner, and central plaques. Cytoplasmic and nuclear microtubules are nucleated from the outer and inner plaques, respectively, whereas the central layer of the SPB plays an important role in tethering the organelle to the nuclear envelope. Associated with one s ...
FGF1 inhibits p53-dependent apoptosis and cell cycle arrest via an
... FGF1 inhibits p53-dependent apoptosis We investigated the relevance of fgf1 gene repression in the p53 pathway by assessing the impact of this factor on p53-dependent apoptosis and cell growth arrest. We transfected REtsAF cells with a pSVL-FGF1 expression vector (p267 (Jaye et al., 1988; Renaud et ...
... FGF1 inhibits p53-dependent apoptosis We investigated the relevance of fgf1 gene repression in the p53 pathway by assessing the impact of this factor on p53-dependent apoptosis and cell growth arrest. We transfected REtsAF cells with a pSVL-FGF1 expression vector (p267 (Jaye et al., 1988; Renaud et ...
regulation of secretion of parathormone and secretory protein
... parathormone secreted was 3.5- to 4-fold greater at 0.5 mM calcium than at 3 .0 mM calcium. At both concentrations, the hormone appeared to be derived from both the previously synthesized and more recently synthesized pools in about the same proportion . The increase in previously synthesized hormon ...
... parathormone secreted was 3.5- to 4-fold greater at 0.5 mM calcium than at 3 .0 mM calcium. At both concentrations, the hormone appeared to be derived from both the previously synthesized and more recently synthesized pools in about the same proportion . The increase in previously synthesized hormon ...
PDF - The Corbo Lab
... lead to ectopic expression in muscle and other tissues. These abnormal sites of expression correspond to sister lineages of the notochord that normally lack Ci-Bra expression. Thus, it would appear that one or more repressors are required to restrict Ci-Bra expression to the developing notochord. In ...
... lead to ectopic expression in muscle and other tissues. These abnormal sites of expression correspond to sister lineages of the notochord that normally lack Ci-Bra expression. Thus, it would appear that one or more repressors are required to restrict Ci-Bra expression to the developing notochord. In ...
histology of organogenic and embryogenic responses in cotyledons
... secondary embryogenesis on medium that lacks plant growth regulators (PGRs) (El Maâtaoui et al. 1990; Fernández-Guijarro et al. 1995). This phenomenon, known as autoembryony, has been described for several species and is the basis of embryo cloning, giving this technique the potential to produce h ...
... secondary embryogenesis on medium that lacks plant growth regulators (PGRs) (El Maâtaoui et al. 1990; Fernández-Guijarro et al. 1995). This phenomenon, known as autoembryony, has been described for several species and is the basis of embryo cloning, giving this technique the potential to produce h ...
Significance of hydrolytic enzymes expressed during xylem
... importance of forests and their value for recreation, tourism and other economical uses of the forest and its products. Understanding wood development may help to increase and optimise yields. The quantitative and qualitative aspects of wood are determined by the properties of the lignocellulosic se ...
... importance of forests and their value for recreation, tourism and other economical uses of the forest and its products. Understanding wood development may help to increase and optimise yields. The quantitative and qualitative aspects of wood are determined by the properties of the lignocellulosic se ...
Lipid Characterization of an Enriched Plasma Membrane
... was insignificant. NAD(P)H-Cyt c reductase activity has been considered characteristic of ER in many plant species (15). This enzyme activity was present in the plasma membrane fraction, indicating that there may have been some ER contamination of the plasma membrane preparation. However, the majori ...
... was insignificant. NAD(P)H-Cyt c reductase activity has been considered characteristic of ER in many plant species (15). This enzyme activity was present in the plasma membrane fraction, indicating that there may have been some ER contamination of the plasma membrane preparation. However, the majori ...
Text - Enlighten: Publications
... the inclusion of a detergent (0.85 mM Triton-X100) in the labeling protocol and noted no discernable difference in labeling results. Control experiments also showed that the labeling was specific. Table 1 provides a summary of antibodies used and labeling results that were obtained for the following ...
... the inclusion of a detergent (0.85 mM Triton-X100) in the labeling protocol and noted no discernable difference in labeling results. Control experiments also showed that the labeling was specific. Table 1 provides a summary of antibodies used and labeling results that were obtained for the following ...
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... 1. One of the seven emergent properties of life is that organisms exhibit homeostasis. Explain ...
... 1. One of the seven emergent properties of life is that organisms exhibit homeostasis. Explain ...
Spatiotemporal Patterning of Reactive Oxygen Production and Ca2+
... (A) Injection of Br2BAPTA (4 mM final intracellular concentration) into the rhizoid cell inhibited mitochondrial ROS production (monitored at 140 s after hyperosmotic treatment) in cells loaded for 20 min with CM-DCFH2-DA but not in the adjacent noninjected thallus cell. The dotted line indicates th ...
... (A) Injection of Br2BAPTA (4 mM final intracellular concentration) into the rhizoid cell inhibited mitochondrial ROS production (monitored at 140 s after hyperosmotic treatment) in cells loaded for 20 min with CM-DCFH2-DA but not in the adjacent noninjected thallus cell. The dotted line indicates th ...
Calcium regulates ricin transport to the Golgi apparatus
... was immunoprecipitated with rabbit anti-Shiga toxin antibodies immobilized on protein A-Sepharose beads (Pharmacia, Piscataway, NJ) overnight at 4°C. The beads were then washed twice with PBS containing 0.35% Triton X-100 before the adsorbed material was analyzed by SDS-PAGE under reducing condition ...
... was immunoprecipitated with rabbit anti-Shiga toxin antibodies immobilized on protein A-Sepharose beads (Pharmacia, Piscataway, NJ) overnight at 4°C. The beads were then washed twice with PBS containing 0.35% Triton X-100 before the adsorbed material was analyzed by SDS-PAGE under reducing condition ...
figure 1 - Open Biology
... binding of GTP– tubulin heterodimers to the microtubule end. Subsequent GTP hydrolysis evokes a conformational change in the heterodimer, resulting in energy being stored as strain within the MT lattice. MT polymerization can do work by overcoming Brownian fluctuations, resulting in the generation o ...
... binding of GTP– tubulin heterodimers to the microtubule end. Subsequent GTP hydrolysis evokes a conformational change in the heterodimer, resulting in energy being stored as strain within the MT lattice. MT polymerization can do work by overcoming Brownian fluctuations, resulting in the generation o ...
The association of sore throat and psoriasis might be explained by
... hyperproliferation driven by an inflammatory infiltrate rich in CD4+, CD8+ and γδ-T cells [2–4]. Psoriasis affects approximately 2% of people of both sexes [5], with diminished quality of life [6] and significant co-morbidities [7]. While psoriasis has been established to be a complex genetic diseas ...
... hyperproliferation driven by an inflammatory infiltrate rich in CD4+, CD8+ and γδ-T cells [2–4]. Psoriasis affects approximately 2% of people of both sexes [5], with diminished quality of life [6] and significant co-morbidities [7]. While psoriasis has been established to be a complex genetic diseas ...
Robust mechanisms of ventral furrow invagination require the
... the types of cell and tissue movements that are possible within a developmental context and/or the types of mechanical regimen that can be used to bring about large-scale changes in tissue organization. To address this question, we have taken a mechanical modelling approach; our central argument bei ...
... the types of cell and tissue movements that are possible within a developmental context and/or the types of mechanical regimen that can be used to bring about large-scale changes in tissue organization. To address this question, we have taken a mechanical modelling approach; our central argument bei ...
- Nottingham ePrints
... to the TE and the inner cell mass (ICM). Derivatives of these two lineages contribute primarily to extraembryonic and embryonic tissues, respectively, leading to the formation of the conceptus. In domestic ungulates, where implantation begins after the second week of embryo development (from day 14 ...
... to the TE and the inner cell mass (ICM). Derivatives of these two lineages contribute primarily to extraembryonic and embryonic tissues, respectively, leading to the formation of the conceptus. In domestic ungulates, where implantation begins after the second week of embryo development (from day 14 ...
1 Introduction of autophagy
... 3. Autophagy is conservative in Eukarya and different types of cells including cancer cells, which is beneficial to cell survival. 4. Dual actions of autophagy in the genesis and development of cancer. a) in the different tumor cells b) impacted by different external factors in the same type of cell ...
... 3. Autophagy is conservative in Eukarya and different types of cells including cancer cells, which is beneficial to cell survival. 4. Dual actions of autophagy in the genesis and development of cancer. a) in the different tumor cells b) impacted by different external factors in the same type of cell ...
TNF-induced endothelial barrier disruption: beyond actin and Rho
... response TNF, also known as TNFα, is a cytokine with pleiotropic effects in many cell types that is expressed as a type II transmembrane protein mainly by macrophages, dendritic cells and T-cells (3, 4). Upon arrival at the plasma membrane, some of the TNF molecules are shed by the metalloproteinase ...
... response TNF, also known as TNFα, is a cytokine with pleiotropic effects in many cell types that is expressed as a type II transmembrane protein mainly by macrophages, dendritic cells and T-cells (3, 4). Upon arrival at the plasma membrane, some of the TNF molecules are shed by the metalloproteinase ...
Using monoclonal antibodies to label living root hairs: a novel tool
... the inclusion of a detergent (0.85 mM Triton-X100) in the labeling protocol and noted no discernable difference in labeling results. Control experiments also showed that the labeling was specific. Table 1 provides a summary of antibodies used and labeling results that were obtained for the following ...
... the inclusion of a detergent (0.85 mM Triton-X100) in the labeling protocol and noted no discernable difference in labeling results. Control experiments also showed that the labeling was specific. Table 1 provides a summary of antibodies used and labeling results that were obtained for the following ...
A cell wall reference profile for Miscanthus bioenergy crops
... understanding of plant cell walls. Identification of desirable cell wall characteristics, and the development of crops containing such features, are crucial steps for lignocellulosic biorefining optimization. However, as all cell walls are not identically composed, it is often difficult to systemati ...
... understanding of plant cell walls. Identification of desirable cell wall characteristics, and the development of crops containing such features, are crucial steps for lignocellulosic biorefining optimization. However, as all cell walls are not identically composed, it is often difficult to systemati ...
The sliding theory of cytoplasmic streaming: fifty years of
... phylogeny and their expression pattern. ACT2, ACT7, and ACT8 are strongly expressed in vegetative tissues, whereas ACT1, ACT3, ACT4, ACT11, and ACT12 are preferentially expressed in reproductive tissues (Meagher et al. 1999). ...
... phylogeny and their expression pattern. ACT2, ACT7, and ACT8 are strongly expressed in vegetative tissues, whereas ACT1, ACT3, ACT4, ACT11, and ACT12 are preferentially expressed in reproductive tissues (Meagher et al. 1999). ...
Cytokinesis defines a spatial landmark for hepatocyte polarization
... division is associated with bile canaliculi formation in vivo. Our study, together with existing evidence, suggests that cytokinesislinked polarization is broadly conserved in diverse cell types. RESULTS Hepatocyte polarization and apical lumen formation are spatially linked to cytokinesis ...
... division is associated with bile canaliculi formation in vivo. Our study, together with existing evidence, suggests that cytokinesislinked polarization is broadly conserved in diverse cell types. RESULTS Hepatocyte polarization and apical lumen formation are spatially linked to cytokinesis ...
Impact of membrane transporters polymorphisms on nucleoside-derived drug bioavailability and action
... Currently used therapeutic nucleoside and nucleotide analogues use the same metabolic pathways as endogenous nucleosides. They enter cells through specific transporters. Once inside the cells, the drug is phosphorylated by a nucleoside kinase and a nucleoside monophosphate kinase. Then a nucleoside ...
... Currently used therapeutic nucleoside and nucleotide analogues use the same metabolic pathways as endogenous nucleosides. They enter cells through specific transporters. Once inside the cells, the drug is phosphorylated by a nucleoside kinase and a nucleoside monophosphate kinase. Then a nucleoside ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.