Regulation of the Eukaryotic Redox-State through - diss.fu
... The origin of life is thought to be based on the formation of compartmentalized autocatalytic chemical cycles. Over time and with the appearance of catalytical biopolymers (ribonucleic acid- or protein-based enzymes), these chemical cycles gained complexity resulting in the evolution of modern metab ...
... The origin of life is thought to be based on the formation of compartmentalized autocatalytic chemical cycles. Over time and with the appearance of catalytical biopolymers (ribonucleic acid- or protein-based enzymes), these chemical cycles gained complexity resulting in the evolution of modern metab ...
Lats kinase is involved in the intestinal apical
... elegans, the intestine, the pharynx and the hypodermis, are separated by the apical surface, bordered by the C. elegans apical junction (Ce_AJ), and have distinct basolateral surfaces that contact both adjacent cells and the underlying basement membranes. The proteins required for cell polarity are ...
... elegans, the intestine, the pharynx and the hypodermis, are separated by the apical surface, bordered by the C. elegans apical junction (Ce_AJ), and have distinct basolateral surfaces that contact both adjacent cells and the underlying basement membranes. The proteins required for cell polarity are ...
Shared versus Specialized Glycinergic Spinal Interneurons in Axial
... elicit in both the free-swimming and fictive preparation with age, this developmental stage was chosen to ensure that larvae would routinely exhibit struggling. All fish were reared in an in-house laboratory facility. Electrophysiology. Larvae were anesthetized in a 0.02% solution of MS222 and then ...
... elicit in both the free-swimming and fictive preparation with age, this developmental stage was chosen to ensure that larvae would routinely exhibit struggling. All fish were reared in an in-house laboratory facility. Electrophysiology. Larvae were anesthetized in a 0.02% solution of MS222 and then ...
High levels of tRNA abundance and alteration of tRNA charging by
... 50 ll 10% SDS, and vortexed at 4 °C for 30 min. After centrifugation, the aqueous layer was extracted with 1 volume of acetate-saturated phenol–chloroform. RNA was precipitated with 1 volume of isopropanol, washed with 75% EtOH/50 mM NaOAc/HOAc (pH 4.8), and resuspended in 10 mM NaOAc/HOAc/1 mM EDTA ...
... 50 ll 10% SDS, and vortexed at 4 °C for 30 min. After centrifugation, the aqueous layer was extracted with 1 volume of acetate-saturated phenol–chloroform. RNA was precipitated with 1 volume of isopropanol, washed with 75% EtOH/50 mM NaOAc/HOAc (pH 4.8), and resuspended in 10 mM NaOAc/HOAc/1 mM EDTA ...
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... However, these studies concluded that the effects of BMP were on the retinal progenitors rather than on Müller glia. We have therefore analyzed the time course of BMP signaling during a period of retinal development in which Müller glial differentiation occurs in mice, the first postnatal week. We f ...
... However, these studies concluded that the effects of BMP were on the retinal progenitors rather than on Müller glia. We have therefore analyzed the time course of BMP signaling during a period of retinal development in which Müller glial differentiation occurs in mice, the first postnatal week. We f ...
LvNotch positions the ectoderm-endoderm boundary
... may also have a role in mediating the position of the ectodermendoderm boundary. LvNotch signaling is activated within presumptive SMCs by underlying micromeres during early development, (Sherwood and McClay, 1999; Sweet et al., 1999; McClay et al., 2000), suggesting that LvNotch may be a component ...
... may also have a role in mediating the position of the ectodermendoderm boundary. LvNotch signaling is activated within presumptive SMCs by underlying micromeres during early development, (Sherwood and McClay, 1999; Sweet et al., 1999; McClay et al., 2000), suggesting that LvNotch may be a component ...
Analysis of Connexin43 phosphorylated at S325, S328 and S330 in
... Furthermore, connexin mutations have been linked to several diseases (Bergoffen et al., 1993; Gong et al., 1997; Kelsell et al., 1997) including oculodentodigital dysplasia, a disease linked to Connexin43 (Cx43) mutations that can cause atrioseptal defects and arrhythmias (Paznekas et al., 2003). Tw ...
... Furthermore, connexin mutations have been linked to several diseases (Bergoffen et al., 1993; Gong et al., 1997; Kelsell et al., 1997) including oculodentodigital dysplasia, a disease linked to Connexin43 (Cx43) mutations that can cause atrioseptal defects and arrhythmias (Paznekas et al., 2003). Tw ...
Debeer S et al, 2013
... human skin Background. Porcine skin is increasingly being employed as a model of human skin in various research fields, including pharmacology, toxicology and immunology, with particular interest in percutaneous permeation and organ transplantation. Porcine skin shows several anatomical and physiolo ...
... human skin Background. Porcine skin is increasingly being employed as a model of human skin in various research fields, including pharmacology, toxicology and immunology, with particular interest in percutaneous permeation and organ transplantation. Porcine skin shows several anatomical and physiolo ...
Identification of GZD824 as an Orally Bioavailable Inhibitor That
... of 10a, compounds 23a, 23b, and 24 were designed and synthesized. Compound 23a lacks a hydrogen bond acceptor in the hinge-binding region. The IC50 values of this compound were 13.9, 82.2, 34.8, 48.6, 29.1, 12.7, 20.1, and 52.6 nM against AblWT, AblT315I, AblE255K, AblG250E, AblQ252H, AblH396P, AblM ...
... of 10a, compounds 23a, 23b, and 24 were designed and synthesized. Compound 23a lacks a hydrogen bond acceptor in the hinge-binding region. The IC50 values of this compound were 13.9, 82.2, 34.8, 48.6, 29.1, 12.7, 20.1, and 52.6 nM against AblWT, AblT315I, AblE255K, AblG250E, AblQ252H, AblH396P, AblM ...
HL-1 Myocytes Exhibit PKC and K Channel-Dependent Delta Opioid Preconditioning
... Streptomycin (Life Technologies), and 10% fetal bovine serum (JRH Biosciences). Norepinephrine supplementation of Claycomb Media TM allows HL-1s to display a beating phenotype and to maintain their differentiated state [28]. HL-1s were plated at a concentration of 2 ⫻ 10 4 cells/cm 2 into 48-well pl ...
... Streptomycin (Life Technologies), and 10% fetal bovine serum (JRH Biosciences). Norepinephrine supplementation of Claycomb Media TM allows HL-1s to display a beating phenotype and to maintain their differentiated state [28]. HL-1s were plated at a concentration of 2 ⫻ 10 4 cells/cm 2 into 48-well pl ...
How and why does β-actin mRNA target?
... mRNA for the assembly of β-actin-specific actin compartments In situ hybridization has shown that β-actin mRNA is localized at the front of cells near the leading edge of lamellipods of crawling cells (Hill and Gunning, 1993; Sundell and Singer, 1990). Furthermore, the localization of β-actin mRNA i ...
... mRNA for the assembly of β-actin-specific actin compartments In situ hybridization has shown that β-actin mRNA is localized at the front of cells near the leading edge of lamellipods of crawling cells (Hill and Gunning, 1993; Sundell and Singer, 1990). Furthermore, the localization of β-actin mRNA i ...
The O-antigen mediates differential survival of
... (Table 1) and in most cases differed from the WT only in carrying a plasmid expressing a fluorescent protein, and/or having the rfb operon replaced so that a different O-antigen was made. A deletion of the rfb operon – D(rfbP-rfbB)3302 – was created by replacing the region between the gnd and galF g ...
... (Table 1) and in most cases differed from the WT only in carrying a plasmid expressing a fluorescent protein, and/or having the rfb operon replaced so that a different O-antigen was made. A deletion of the rfb operon – D(rfbP-rfbB)3302 – was created by replacing the region between the gnd and galF g ...
Epigenetic and transcriptional regulation of early mouse development
... Structure of the Elongator complex ..................................................................................................... 18 Diverse functions of the Elongator complex .................................................................................... 19 Transcriptional regulation of ...
... Structure of the Elongator complex ..................................................................................................... 18 Diverse functions of the Elongator complex .................................................................................... 19 Transcriptional regulation of ...
Two-Piece Hard Capsules for Pharmaceutical
... While two-piece capsules may be comprised of various materials, the vast majority are still made using high-quality gelatin prepared from collagen. Collagen is a fibrillary protein that contains 18 different amino acids. The collagen of bones and hides is subject to a maceration and purification pro ...
... While two-piece capsules may be comprised of various materials, the vast majority are still made using high-quality gelatin prepared from collagen. Collagen is a fibrillary protein that contains 18 different amino acids. The collagen of bones and hides is subject to a maceration and purification pro ...
Mast Cell-Derived Exosomes Induce Phenotypic and Functional
... DCs of human tonsil tissue in vivo (8), which does not express these molecules. Tolerosomes assembled in and released from the small intestinal epithelial cells are another example of exosomelike structures (9). Tolerosomes isolated from serum shortly after Ag feeding or from in vitro-pulsed intesti ...
... DCs of human tonsil tissue in vivo (8), which does not express these molecules. Tolerosomes assembled in and released from the small intestinal epithelial cells are another example of exosomelike structures (9). Tolerosomes isolated from serum shortly after Ag feeding or from in vitro-pulsed intesti ...
Lessons on longevity from budding yeast
... The past decade has seen fundamental advances in our understanding of the ageing process and raised optimism that interventions to slow ageing may be on the horizon. Studies of budding yeast have made immense contributions to this progress. Yeast longevity factors have now been shown to modulate age ...
... The past decade has seen fundamental advances in our understanding of the ageing process and raised optimism that interventions to slow ageing may be on the horizon. Studies of budding yeast have made immense contributions to this progress. Yeast longevity factors have now been shown to modulate age ...
EGF receptor signaling pathway Drosophila activation
... Figure 2. ave is required for EGFR signaling during eye development. ave mutant clones generated in third instar eye discs are marked by loss of GFP (B,E,H,K,N,Q,T; green in C,F,I,L,O,R,U). (A–C) Cone cells were stained with anti-Cut (A, red in C) and photoreceptors with anti-Elav (blue in C). Very ...
... Figure 2. ave is required for EGFR signaling during eye development. ave mutant clones generated in third instar eye discs are marked by loss of GFP (B,E,H,K,N,Q,T; green in C,F,I,L,O,R,U). (A–C) Cone cells were stained with anti-Cut (A, red in C) and photoreceptors with anti-Elav (blue in C). Very ...
1. (a) cells if more than one box is ticked, award no mark 1 (b) tail 1
... it prevents bacteria or viruses from entering accept ‘microbes’ or ‘germs’ for bacteria these less precise terms are accepted if the answer indicates that the scab acts as a barrier ‘to keep it clean’ is insufficient accept ‘it stops dirt getting in’ accept ‘it stops infection’ accept ‘it stops di ...
... it prevents bacteria or viruses from entering accept ‘microbes’ or ‘germs’ for bacteria these less precise terms are accepted if the answer indicates that the scab acts as a barrier ‘to keep it clean’ is insufficient accept ‘it stops dirt getting in’ accept ‘it stops infection’ accept ‘it stops di ...
The Role of Lysosomes in Molluscan Inflammation1
... these animals. At this point, a seldom responses are characterized by an infiltraremembered fact relative to inflammation tion of lymphocytes and cells of the monoin molluscs bears recalling. Specifically, cyte-macrophage lineage, which are Hurst and Walker (1933, 1935) have actively phagocytic. It ...
... these animals. At this point, a seldom responses are characterized by an infiltraremembered fact relative to inflammation tion of lymphocytes and cells of the monoin molluscs bears recalling. Specifically, cyte-macrophage lineage, which are Hurst and Walker (1933, 1935) have actively phagocytic. It ...
Aquatic Microbial Ecology 58:303
... 23 February, the concentration of chl a showed an obvious diel change, basically similar to that of total bacterial abundance (Fig. 2c). The highest concentrations of chl a were measured at 18:00 to 21:00 h, and the lowest values were at midnight or just before dawn. Also, during the day there was a ...
... 23 February, the concentration of chl a showed an obvious diel change, basically similar to that of total bacterial abundance (Fig. 2c). The highest concentrations of chl a were measured at 18:00 to 21:00 h, and the lowest values were at midnight or just before dawn. Also, during the day there was a ...
here - The Nebenführ Lab
... the Golgi to the ER and intra-Golgi transport is mediated by COPI-coated vesicles. The formation of these vesicles is basically a two-step process involving the sequential recruitment of a GTPase and a preformed coat protein complex, both of which are located mainly in the cytosol (Harter, 1999; Nic ...
... the Golgi to the ER and intra-Golgi transport is mediated by COPI-coated vesicles. The formation of these vesicles is basically a two-step process involving the sequential recruitment of a GTPase and a preformed coat protein complex, both of which are located mainly in the cytosol (Harter, 1999; Nic ...
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... expression increased in control cells; this increment was lost in Hif1␣depleted cells. Furthermore, under normoxic conditions Hif1␣ overexpression induced an increase in Sox9 expression. Chromatin immunoprecipitation (ChIP) assay provided evidence for direct interaction of HIF1␣ with the Sox9 promot ...
... expression increased in control cells; this increment was lost in Hif1␣depleted cells. Furthermore, under normoxic conditions Hif1␣ overexpression induced an increase in Sox9 expression. Chromatin immunoprecipitation (ChIP) assay provided evidence for direct interaction of HIF1␣ with the Sox9 promot ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.