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... fixation, these experimental embryos were screened to ensure that they contained no cells labelled with FDA, the lineage tracer used for the ablations. Preparation of embryos with cleavage-arrested teloblasts or proteloblasts To prevent the formation of ectodermal bandlets, mesodermal bandlets or en ...
... fixation, these experimental embryos were screened to ensure that they contained no cells labelled with FDA, the lineage tracer used for the ablations. Preparation of embryos with cleavage-arrested teloblasts or proteloblasts To prevent the formation of ectodermal bandlets, mesodermal bandlets or en ...
Oriented cell motility and division underlie early limb bud
... and methods) were rarely observed in CAG::H2B-EGFP transgenic tissues during live-image data acquisition. As forelimb and hindlimb development might be subject to distinct kinetics, we chose to focus our studies on the forelimb. During Theiler stage 14 (~E9.0-9.25, 18-20 somites) the prospective mou ...
... and methods) were rarely observed in CAG::H2B-EGFP transgenic tissues during live-image data acquisition. As forelimb and hindlimb development might be subject to distinct kinetics, we chose to focus our studies on the forelimb. During Theiler stage 14 (~E9.0-9.25, 18-20 somites) the prospective mou ...
High-Performance Exosome Purification
... apoptotic bodies. Classification of membrane vesicles— and the most appropriate, and effective protocols for their isolation—continue to be intense areas of investigation. When isolating vesicles, it is crucial to use systems that are able to separate them, to avoid cross-contamination. At the same ...
... apoptotic bodies. Classification of membrane vesicles— and the most appropriate, and effective protocols for their isolation—continue to be intense areas of investigation. When isolating vesicles, it is crucial to use systems that are able to separate them, to avoid cross-contamination. At the same ...
Insights into the Role of Specific Lipids in the
... and signal transduction (Simons and Ikonen, 1997; Brown and London, 1998; Keller and Simons, 1998; Simons and Toomre, 2000; Simons and Vaz, 2004; Hancock, 2006). Similar microdomains in the PM of yeast (Saccharomyces cerevisiae) cells, enriched in ergosterol, have also been reported (Bagnat et al., ...
... and signal transduction (Simons and Ikonen, 1997; Brown and London, 1998; Keller and Simons, 1998; Simons and Toomre, 2000; Simons and Vaz, 2004; Hancock, 2006). Similar microdomains in the PM of yeast (Saccharomyces cerevisiae) cells, enriched in ergosterol, have also been reported (Bagnat et al., ...
Co-Expression of Arabidopsis thaliana Phytochelatin Synthase and Treponema denticola
... significant growth inhibition was observed in the presence of cysteine (Table I), even though the presence of cysteine desulfhydrase partially reduced the level of growth inhibition. While cells expressing cysteine desulfhydrase accumulated 25% more arsenite than the wild-type strain BY4742 even in t ...
... significant growth inhibition was observed in the presence of cysteine (Table I), even though the presence of cysteine desulfhydrase partially reduced the level of growth inhibition. While cells expressing cysteine desulfhydrase accumulated 25% more arsenite than the wild-type strain BY4742 even in t ...
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... Staphylococcus aureus. (A) M. tuberculosis initially resides in a phagosome characterized by the presence Staphylococcus aureus. (A) M. tuberculosis initially resides in a phagosome characterized by the of Rab5, Rab10, Rab22b and Rab23 that matures to a late compartment. Approximately 1 h p.i. Mtb p ...
... Staphylococcus aureus. (A) M. tuberculosis initially resides in a phagosome characterized by the presence Staphylococcus aureus. (A) M. tuberculosis initially resides in a phagosome characterized by the of Rab5, Rab10, Rab22b and Rab23 that matures to a late compartment. Approximately 1 h p.i. Mtb p ...
Chitosan Flocculation-sedimentation for Harvesting Selected
... 2015). The other prospective microalgae species for biodiesel production is mixed cultures strain, such as mixed cultures microalgae from South Coast of Yogyakarta. The identified microalgae species of mixed cultures from South Coast of Yogyakarta are Cyclotella polymorpha and Chlamydomonas sp., whi ...
... 2015). The other prospective microalgae species for biodiesel production is mixed cultures strain, such as mixed cultures microalgae from South Coast of Yogyakarta. The identified microalgae species of mixed cultures from South Coast of Yogyakarta are Cyclotella polymorpha and Chlamydomonas sp., whi ...
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... dissection of specific somites or other tissues which in most cases were transferred to a Ca2+/Mg2+-free Steinberg's solution containing 0-4mM-EDTA (Sigma) for 20-30 min. The dissociated tissues then were drawn up through a fine, flame-drawn Pasteur pipette and dispersed into localized areas of cult ...
... dissection of specific somites or other tissues which in most cases were transferred to a Ca2+/Mg2+-free Steinberg's solution containing 0-4mM-EDTA (Sigma) for 20-30 min. The dissociated tissues then were drawn up through a fine, flame-drawn Pasteur pipette and dispersed into localized areas of cult ...
The Role of the Arabidopsis ELD1 Gene in Cell
... processes: division, growth, and differentiation. Changes in cell division and cell growth processes or their control affect the growth and differentiation of the entire plant. Plants with altered cell division and growth often display abnormal morphology and alterations in organogenesis and interna ...
... processes: division, growth, and differentiation. Changes in cell division and cell growth processes or their control affect the growth and differentiation of the entire plant. Plants with altered cell division and growth often display abnormal morphology and alterations in organogenesis and interna ...
Receptor Activation and Homer Differentially Control the Lateral
... (Mary et al., 1998). cDNAs corresponding to Homer1b were synthesized from mouse brain total RNAs and reverse-transcribed with avian myeloblastosis virus RNA polymerase using the reverse primer 5⬘-GGATCC GCGC TGCATTCC -AGTAGC TTGGC -3⬘ containing a BamHI site and corresponding to the end of rat Homer ...
... (Mary et al., 1998). cDNAs corresponding to Homer1b were synthesized from mouse brain total RNAs and reverse-transcribed with avian myeloblastosis virus RNA polymerase using the reverse primer 5⬘-GGATCC GCGC TGCATTCC -AGTAGC TTGGC -3⬘ containing a BamHI site and corresponding to the end of rat Homer ...
Pre-operative HBO treatment led to enhanced expression of
... nuclei may induce the apoptosis of hepatocytes in vivo, enhanced GM3 expression around the nuclei could have important implications. Namely, if GM3 molecules are more intensively expressed, their precursors ceramide and sphingosine would be present in a smaller amount. Therefore, the apoptosis durin ...
... nuclei may induce the apoptosis of hepatocytes in vivo, enhanced GM3 expression around the nuclei could have important implications. Namely, if GM3 molecules are more intensively expressed, their precursors ceramide and sphingosine would be present in a smaller amount. Therefore, the apoptosis durin ...
The effects of bleomycin, mitomycin C, and in vitro in vivo
... supervision the work on haemangioma biopsies was conducted. I also thank him for his input through-out the study. Prof Nola Dippenaar for all her input and support during the HPLC work. I also thank her for all the helpful suggestions and the role she played during the editing of this thesis. I am i ...
... supervision the work on haemangioma biopsies was conducted. I also thank him for his input through-out the study. Prof Nola Dippenaar for all her input and support during the HPLC work. I also thank her for all the helpful suggestions and the role she played during the editing of this thesis. I am i ...
Endosomes/Lysosomes in Macrophages Fusion between
... proposed (reviewed in Refs. 4, 12, and 13). Immunity is central to the self-defense mechanism, which can be divided into the innate and adaptive immune responses. Innate immunity is defined as a mechanism by which self and non-self are discriminated without involvement of proteins encoded by genes t ...
... proposed (reviewed in Refs. 4, 12, and 13). Immunity is central to the self-defense mechanism, which can be divided into the innate and adaptive immune responses. Innate immunity is defined as a mechanism by which self and non-self are discriminated without involvement of proteins encoded by genes t ...
Syntaxin of Plant Proteins SYP123 and SYP132 Mediate Root Hair
... focal accumulation of GFP–SYP123 in the root hair tip. As shown in Fig. 3A and B, LatB treatment gradually reduced the fluorescence intensity of GFP–SYP123 in the root hair region, but no difference was found in the root hair cell region. After 30 min of treatment, the focal accumulation of GFP–SYP1 ...
... focal accumulation of GFP–SYP123 in the root hair tip. As shown in Fig. 3A and B, LatB treatment gradually reduced the fluorescence intensity of GFP–SYP123 in the root hair region, but no difference was found in the root hair cell region. After 30 min of treatment, the focal accumulation of GFP–SYP1 ...
Characterization and Biological Activities of Ocellatin Peptides from
... pro-hormone processing signal40 (Table 2). The mature peptides are at the C-terminus and consist of 25 or 32 amino acids that differ little compared to other peptides found in skin secretions of other members of the Leptodactylus genus. Sequence Analysis. The primary structures of the new ocellatin p ...
... pro-hormone processing signal40 (Table 2). The mature peptides are at the C-terminus and consist of 25 or 32 amino acids that differ little compared to other peptides found in skin secretions of other members of the Leptodactylus genus. Sequence Analysis. The primary structures of the new ocellatin p ...
LIPOSOMES FOR THE DELIVERY OF NUCLEIC ACID DRUGS A
... liposomes have received attention not only for their utility as model membrane systems, but also for use in drug delivery. Typically, liposomes are used as drug carriers, with the solubilized drug encapsulated in the internal aqueous space formed by the liposomal lamellae. Liposomal drug formulation ...
... liposomes have received attention not only for their utility as model membrane systems, but also for use in drug delivery. Typically, liposomes are used as drug carriers, with the solubilized drug encapsulated in the internal aqueous space formed by the liposomal lamellae. Liposomal drug formulation ...
Hydrogels for protein delivery in tissue engineering ⁎ Roberta Censi
... growth factors and their applications in tissue engineering are listed in Table 1. Unlike other proteins (i.e. hormones), growth factors act locally and possess short diffusion distances through the ECMs, owing to their short half-lives. Typically, growth factors are enzymatically or chemically degr ...
... growth factors and their applications in tissue engineering are listed in Table 1. Unlike other proteins (i.e. hormones), growth factors act locally and possess short diffusion distances through the ECMs, owing to their short half-lives. Typically, growth factors are enzymatically or chemically degr ...
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... able to produce new gametes that can start the cycle again. In this circularly repetitive process, the zygote stands out as a unique cell in an essential aspect: it has the potential to generate all cells and tissues necessaries (embryonic and extraembryonic) to form the new organism when implanted ...
... able to produce new gametes that can start the cycle again. In this circularly repetitive process, the zygote stands out as a unique cell in an essential aspect: it has the potential to generate all cells and tissues necessaries (embryonic and extraembryonic) to form the new organism when implanted ...
Doehrty-McMahon 2009
... to the clarity beginning to emerge from studies of endocytic events, we urge abandonment of the term RME because methods are readily available to easily dissect the method(s) of endocytosis used for the cargo of interest; a scheme for endocytic classification can be found in Reference 6 (see also Fig ...
... to the clarity beginning to emerge from studies of endocytic events, we urge abandonment of the term RME because methods are readily available to easily dissect the method(s) of endocytosis used for the cargo of interest; a scheme for endocytic classification can be found in Reference 6 (see also Fig ...
NO 3
... the soil medium, the contact often being enhanced by numerous root hairs. Water free space(水分自由空间） and Donnan free space（ 杜南自由空间） Cations interact with the fixed anions in the cell wall (pectin and proteins) and hence their motion is much restricted. The part of the apoplast is also called apparent ...
... the soil medium, the contact often being enhanced by numerous root hairs. Water free space(水分自由空间） and Donnan free space（ 杜南自由空间） Cations interact with the fixed anions in the cell wall (pectin and proteins) and hence their motion is much restricted. The part of the apoplast is also called apparent ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.