Somite dorsoventral polarity - Development
... ED2 quail donor embryos were cut from the yolk in a bowl containing Tyrode’s salts (Sigma) with iridectomy scissors (Fine Science Tools) and pinned dorsal side up in a glass dish coated with black Sylgard (Fig. 2A). Under a dissecting microscope, incisions in the ectoderm were made with tungsten mic ...
... ED2 quail donor embryos were cut from the yolk in a bowl containing Tyrode’s salts (Sigma) with iridectomy scissors (Fine Science Tools) and pinned dorsal side up in a glass dish coated with black Sylgard (Fig. 2A). Under a dissecting microscope, incisions in the ectoderm were made with tungsten mic ...
Positive Regulation of IκB Kinase Signaling by Protein
... the wild type and PP2A-binding defective IKKγ constructs were efficiently integrated into endogenous IKK complexes containing the IKKα and IKKβ catalytic subunits. ...
... the wild type and PP2A-binding defective IKKγ constructs were efficiently integrated into endogenous IKK complexes containing the IKKα and IKKβ catalytic subunits. ...
Mitochondria in Lung Biology and Pathology: More than - AJP-Lung
... Mitochondrial oxygen sensing Mitochondria consume O2 at cytochrome c oxidase, the terminal complex in the ...
... Mitochondrial oxygen sensing Mitochondria consume O2 at cytochrome c oxidase, the terminal complex in the ...
the selective interruption of nucleolar rna synthesis in hela cells by
... thymidine incorporation (DNA synthesis) and amino acid incorporation (protein synthesis) are unaffected at 50 µg/ml . The insensitivity of DNA and protein synthesis shows that 3'dA does not directly interfere with general cell metabolism . The principal site of action of 3'dA is the nucleolus . Cell ...
... thymidine incorporation (DNA synthesis) and amino acid incorporation (protein synthesis) are unaffected at 50 µg/ml . The insensitivity of DNA and protein synthesis shows that 3'dA does not directly interfere with general cell metabolism . The principal site of action of 3'dA is the nucleolus . Cell ...
Distinct Protease Requirements for Antigen Presentation In Vitro and
... endopeptidase (AEP). Presentation of myoglobin (8), OVA (6), and the (161–175) epitope of the MAGE-3 tumor Ag (9) was more efficient in dendritic cells (DCs) or splenocytes treated with an aspartyl protease inhibitor or lacking cathepsin D, suggesting that destructive processing by this enzyme can o ...
... endopeptidase (AEP). Presentation of myoglobin (8), OVA (6), and the (161–175) epitope of the MAGE-3 tumor Ag (9) was more efficient in dendritic cells (DCs) or splenocytes treated with an aspartyl protease inhibitor or lacking cathepsin D, suggesting that destructive processing by this enzyme can o ...
Peptide Repertoire Class I Molecule Q10 Binds a Classical The
... The following pairs of primers were used to amplify parts of H-2 molecules: for the ␣1␣2 region of Q10, (P1) 5⬘-AAACCCGTCGACGATC CCAGATGGGGGCGATGGCG-3⬘ (signal peptide sequence in bold, SalI site underlined) and (P2) 5⬘-AAACCCAGATCTGTGCGCAG CAGCGTCT-3⬘ (C-terminal part of ␣2 domain in bold, BglII si ...
... The following pairs of primers were used to amplify parts of H-2 molecules: for the ␣1␣2 region of Q10, (P1) 5⬘-AAACCCGTCGACGATC CCAGATGGGGGCGATGGCG-3⬘ (signal peptide sequence in bold, SalI site underlined) and (P2) 5⬘-AAACCCAGATCTGTGCGCAG CAGCGTCT-3⬘ (C-terminal part of ␣2 domain in bold, BglII si ...
histo compendium team deogy fall 2012 121130
... compact and marrow showing. 3)Bone tissue. Light micrograph of a close-up of a longitudinal polished section through human bone. 4)Ground cross section of human Compact Bone stained with India ink. HC, Haversion Canal, canaliculus; dotted line, perimeter of Osteon. x700. 5) Bone lacuna. Coloured sca ...
... compact and marrow showing. 3)Bone tissue. Light micrograph of a close-up of a longitudinal polished section through human bone. 4)Ground cross section of human Compact Bone stained with India ink. HC, Haversion Canal, canaliculus; dotted line, perimeter of Osteon. x700. 5) Bone lacuna. Coloured sca ...
Accepted version - QMRO Home - Queen Mary University of London
... Although these data are based on comparisons of quite narrow ranges of donor cell survival (only <5% at the initial points studied), these may suggest that allogeneic MSCs can survive for a certain early period after transplantation, but are eventually rejected in a long term. We have recently repor ...
... Although these data are based on comparisons of quite narrow ranges of donor cell survival (only <5% at the initial points studied), these may suggest that allogeneic MSCs can survive for a certain early period after transplantation, but are eventually rejected in a long term. We have recently repor ...
Plasma Membrane Ghosts Form Differently When Produced from
... (Giddings and Staehelin 1991). That these cortical microtubules closely associate with the plasma membrane has been demonstrated by numerous microtubule ghosting studies in which isolated patches of plasma membrane retain cytoskeletal elements after the cortical cytoplasm has been washed away (March ...
... (Giddings and Staehelin 1991). That these cortical microtubules closely associate with the plasma membrane has been demonstrated by numerous microtubule ghosting studies in which isolated patches of plasma membrane retain cytoskeletal elements after the cortical cytoplasm has been washed away (March ...
S. cerevisiae Mitotic Exit Activator
... 2001). This then promotes Dbf2-Mobl localization to the SPB (Komarnitsky et al., 1998; Luca and Winey, 1998; Mah et al., 2001). The localization of CdclS5and Dbf2-Mobl to the SPB is thought to be important for the kinase activity of these proteins (Visintin and Amon, 2001). It is Cdc14 that ultimate ...
... 2001). This then promotes Dbf2-Mobl localization to the SPB (Komarnitsky et al., 1998; Luca and Winey, 1998; Mah et al., 2001). The localization of CdclS5and Dbf2-Mobl to the SPB is thought to be important for the kinase activity of these proteins (Visintin and Amon, 2001). It is Cdc14 that ultimate ...
The physical basis of active mechanosensitivity by the hair
... currents show no sign of fast adaptation or Ca2þ sensitivity [42]. Alternatively, a protein associated with the channel may change conformation [31] or reduce its stiffness [43] to relax tip-link tension and in turn allow channel reclosure. The involvement of myosin 1c in fast adaptation points to t ...
... currents show no sign of fast adaptation or Ca2þ sensitivity [42]. Alternatively, a protein associated with the channel may change conformation [31] or reduce its stiffness [43] to relax tip-link tension and in turn allow channel reclosure. The involvement of myosin 1c in fast adaptation points to t ...
dravhandling-hafte - DUO
... epithelial cell line in culture is the Madin-Darby canine kidney (MDCK) cell line, isolated from a dog kidney cortex in 1958. In the textbook view, proteins destined for the apical and basolateral membrane domains travel a common route through the secretory pathway to the trans-Golgi network, the ex ...
... epithelial cell line in culture is the Madin-Darby canine kidney (MDCK) cell line, isolated from a dog kidney cortex in 1958. In the textbook view, proteins destined for the apical and basolateral membrane domains travel a common route through the secretory pathway to the trans-Golgi network, the ex ...
Figures and figure supplements
... numbers (see Table 2) are indicated in red next to the pathway that was mutated. Known bacterial enzymes appear in italic. Trp - tryptophan, IAM indole-3-acetamide, Ipy - indole-3-pyruvate, IAAId - indole-3-acetaldehyde. Image modified from Spaepen et al. (2007). (b) Mutants in various biosynthetic ...
... numbers (see Table 2) are indicated in red next to the pathway that was mutated. Known bacterial enzymes appear in italic. Trp - tryptophan, IAM indole-3-acetamide, Ipy - indole-3-pyruvate, IAAId - indole-3-acetaldehyde. Image modified from Spaepen et al. (2007). (b) Mutants in various biosynthetic ...
The Crabtree Effect: A Review
... error because of the lack of control of pH change (50). Methylene blue also releases the inhibition (87, 56, 80, 95). It has been shown by Racker (80) and Wenner et al. (96) that the methylene blue effect may be due to the extramitochondrial stimulation of glucose oxidation. An important point about ...
... error because of the lack of control of pH change (50). Methylene blue also releases the inhibition (87, 56, 80, 95). It has been shown by Racker (80) and Wenner et al. (96) that the methylene blue effect may be due to the extramitochondrial stimulation of glucose oxidation. An important point about ...
Influence of Bacillus subtilis Cell Walls and EDTA on Calcite
... This study investigates the influence of EDTA and the Gram-positive cell walls of Bacillus subtilis on the dissolution rates and development of morphological features on the calcite {101h4} surface. The calcite dissolution rates are compared at equivalent saturation indicies (SI) and relative to its ...
... This study investigates the influence of EDTA and the Gram-positive cell walls of Bacillus subtilis on the dissolution rates and development of morphological features on the calcite {101h4} surface. The calcite dissolution rates are compared at equivalent saturation indicies (SI) and relative to its ...
O B RGANIC
... Generic variables, like x, y and z are used in different contexts throughout the text. ...
... Generic variables, like x, y and z are used in different contexts throughout the text. ...
View Full Page PDF
... Holthuis, Joost C. M., Thomas Pomorski, René J. Raggers, Hein Sprong, and Gerrit van Meer. The Organizing Potential of Sphingolipids in Intracellular Membrane Transport. Physiol Rev 81: 1689 –1723, 2001.— Eukaryotes are characterized by endomembranes that are connected by vesicular transport along ...
... Holthuis, Joost C. M., Thomas Pomorski, René J. Raggers, Hein Sprong, and Gerrit van Meer. The Organizing Potential of Sphingolipids in Intracellular Membrane Transport. Physiol Rev 81: 1689 –1723, 2001.— Eukaryotes are characterized by endomembranes that are connected by vesicular transport along ...
Emergent Synchronous Bursting of Oxytocin Neuronal Network
... When young suckle, they are rewarded intermittently with a let-down of milk that results from reflex secretion of the hormone oxytocin; without oxytocin, newly born young will die unless they are fostered. Oxytocin is made by magnocellular hypothalamic neurons, and is secreted from their nerve endin ...
... When young suckle, they are rewarded intermittently with a let-down of milk that results from reflex secretion of the hormone oxytocin; without oxytocin, newly born young will die unless they are fostered. Oxytocin is made by magnocellular hypothalamic neurons, and is secreted from their nerve endin ...
PDF
... In section 1 of the Results, it has been shown that there is excess embryonic mortality between days 6 and 8, amounting to 56-1-43-2 = 12-9% (Table 6). In 12-9 % of the day 8 implants (8.4 of Table 6), embryonic structures were seen. In 8-8 % of the implants at day 6 there were embryonic cell clumps ...
... In section 1 of the Results, it has been shown that there is excess embryonic mortality between days 6 and 8, amounting to 56-1-43-2 = 12-9% (Table 6). In 12-9 % of the day 8 implants (8.4 of Table 6), embryonic structures were seen. In 8-8 % of the implants at day 6 there were embryonic cell clumps ...
Macrophage-pathogen interactions in infectious diseases: new
... Fig. 1. Evasion of macrophage defense mechanisms by intracellular pathogens. Upon phagocytosis (1), the pathogens generally reside within phagosomal compartments where a plethora of microbicidal components cooperate in a multidirectional assault to the microbes (2). By transferring virulence factors ...
... Fig. 1. Evasion of macrophage defense mechanisms by intracellular pathogens. Upon phagocytosis (1), the pathogens generally reside within phagosomal compartments where a plethora of microbicidal components cooperate in a multidirectional assault to the microbes (2). By transferring virulence factors ...
PDF
... fixation, these experimental embryos were screened to ensure that they contained no cells labelled with FDA, the lineage tracer used for the ablations. Preparation of embryos with cleavage-arrested teloblasts or proteloblasts To prevent the formation of ectodermal bandlets, mesodermal bandlets or en ...
... fixation, these experimental embryos were screened to ensure that they contained no cells labelled with FDA, the lineage tracer used for the ablations. Preparation of embryos with cleavage-arrested teloblasts or proteloblasts To prevent the formation of ectodermal bandlets, mesodermal bandlets or en ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.