AHEART December 46/6 - AJP
... before use. Warmed peptide stock solutions were diluted directly into cell suspensions. R-PIA was dissolved directly into the cell suspension buffer. Unless otherwise stated, all drugs were administered as 100 µM final concentration. This dosage was chosen because Armstrong et al. (3) required 100 µ ...
... before use. Warmed peptide stock solutions were diluted directly into cell suspensions. R-PIA was dissolved directly into the cell suspension buffer. Unless otherwise stated, all drugs were administered as 100 µM final concentration. This dosage was chosen because Armstrong et al. (3) required 100 µ ...
p-Glycoprotein-Mediated Transport of a Fluorescent Rapamycin
... similar to those shown to be effective for other MDR reversal agents, such as CSA and verapamil. These findings document an interaction between rapamycin and p-glycoprotein, but they do not address the question of whether the drug is a substrate for transport. In the present study we used confocal m ...
... similar to those shown to be effective for other MDR reversal agents, such as CSA and verapamil. These findings document an interaction between rapamycin and p-glycoprotein, but they do not address the question of whether the drug is a substrate for transport. In the present study we used confocal m ...
Control of convergent yolk syncytial layer nuclear movement in
... Rink et al., 2005). Net dorsal speed was calculated by dividing the net displacement in the x-axis by the total time. We used the emerging notochord and neural tube as anatomical landmarks to determine the embryonic midline. Movement analysis in three dimensions ...
... Rink et al., 2005). Net dorsal speed was calculated by dividing the net displacement in the x-axis by the total time. We used the emerging notochord and neural tube as anatomical landmarks to determine the embryonic midline. Movement analysis in three dimensions ...
Inhibition of Smooth Muscle Cells Proliferation Using Sirolimus
... 1% Ab-Am, for day 3 and day 7, respectively. After 24 hours, SMCs were treated with Sirolimus-loaded PgP (SPgP-50µg/ml, Sirolimus-2.6µg/ml) and Sirolimus alone (dissolved in DMSO) for 5, 15, 30 and 60 minutes. After treatment, DMEM containing Sirolimus was removed and replenished with fresh DMEM wit ...
... 1% Ab-Am, for day 3 and day 7, respectively. After 24 hours, SMCs were treated with Sirolimus-loaded PgP (SPgP-50µg/ml, Sirolimus-2.6µg/ml) and Sirolimus alone (dissolved in DMSO) for 5, 15, 30 and 60 minutes. After treatment, DMEM containing Sirolimus was removed and replenished with fresh DMEM wit ...
Autoantibody Explosion in Systemic Lupus Erythematosus: More
... antiribosomal P proteins antibodies associated with nephritis and central nervous system (CNS) involvement. In contrast, many other autoantibodies have no correlation with disease activity or any clinical manifestation of SLE. Another important issue is the specificity of the different autoantibodie ...
... antiribosomal P proteins antibodies associated with nephritis and central nervous system (CNS) involvement. In contrast, many other autoantibodies have no correlation with disease activity or any clinical manifestation of SLE. Another important issue is the specificity of the different autoantibodie ...
Force development by the contractile vacuole
... Fig. 3. (A) Schematic drawing of the microcantilever system for measuring the force generated by an in vitro CV of P. multimicronucleatum. The CV, surrounded by a small amount of the cytosol, was placed at the tip of the holding pipette by lowering the hydrostatic pressure inside the pipette. (B) Th ...
... Fig. 3. (A) Schematic drawing of the microcantilever system for measuring the force generated by an in vitro CV of P. multimicronucleatum. The CV, surrounded by a small amount of the cytosol, was placed at the tip of the holding pipette by lowering the hydrostatic pressure inside the pipette. (B) Th ...
Control of convergent yolk syncytial layer nuclear movement in
... Rink et al., 2005). Net dorsal speed was calculated by dividing the net displacement in the x-axis by the total time. We used the emerging notochord and neural tube as anatomical landmarks to determine the embryonic midline. Movement analysis in three dimensions ...
... Rink et al., 2005). Net dorsal speed was calculated by dividing the net displacement in the x-axis by the total time. We used the emerging notochord and neural tube as anatomical landmarks to determine the embryonic midline. Movement analysis in three dimensions ...
Promiscuous and specific bacterial symbiont acquisition in the
... Multicellular organisms usually are associated with more than one bacterial species. Ectosymbionts form entire assemblages which are designated as microbiota of the respective host. The microbiota of very ‘simple’ animals like the cnidarians Hydra (Fraune and Bosch 2007; Franzenburg et al. 2013) and ...
... Multicellular organisms usually are associated with more than one bacterial species. Ectosymbionts form entire assemblages which are designated as microbiota of the respective host. The microbiota of very ‘simple’ animals like the cnidarians Hydra (Fraune and Bosch 2007; Franzenburg et al. 2013) and ...
The Arf and Rab11 effector FIP3 acts synergistically with ASAP1 to
... ASAP1 and Rab11a during its passage out of the Golgi and TGN. (A) The schematic structure of FIP3. ASAP1, Arf and Rab11 binding domains (BD) are indicated. (B) FIP3F1 (F1; amino acids 441–756) and FIP3C (C; amino acids 666–756) were expressed as GST fusion proteins and interacting retinal proteins w ...
... ASAP1 and Rab11a during its passage out of the Golgi and TGN. (A) The schematic structure of FIP3. ASAP1, Arf and Rab11 binding domains (BD) are indicated. (B) FIP3F1 (F1; amino acids 441–756) and FIP3C (C; amino acids 666–756) were expressed as GST fusion proteins and interacting retinal proteins w ...
Micronuclei Bearing Acentric Extrachromosomal Chromatin Are
... FIGURE 2. Visualization of transcription from DMs in live cells. A and B. Metaphase chromosome prepared from C4C4 cells were hybridized with digoxigenin-labeled pECMS2h DNA and biotin-labeled DHFR IR DNA. The hybridized probes were detected by FITC and Texas red, respectively, and DNA was countersta ...
... FIGURE 2. Visualization of transcription from DMs in live cells. A and B. Metaphase chromosome prepared from C4C4 cells were hybridized with digoxigenin-labeled pECMS2h DNA and biotin-labeled DHFR IR DNA. The hybridized probes were detected by FITC and Texas red, respectively, and DNA was countersta ...
Fcg Receptor Deficiency Attenuates Diabetic Nephropathy
... adaptive immunity. Most forms of modified LDL are immunogenic and induce autoantibody production; these proinflammatory properties are greatly increased by the formation of LDL-containing immune complexes.8 This adaptive immune response to modified LDL has also been reported in mice.9 Patients with typ ...
... adaptive immunity. Most forms of modified LDL are immunogenic and induce autoantibody production; these proinflammatory properties are greatly increased by the formation of LDL-containing immune complexes.8 This adaptive immune response to modified LDL has also been reported in mice.9 Patients with typ ...
Maternal Torso-Like Coordinates Tissue Folding During
... additional large field of cells posterior to the pole cells was observed (Fig. 2I). We reasoned that these cells might be mesodermal tissue remaining at the embryo surface as a consequence of invagination failure. To confirm this, we stained tslΔ embryos with anti-Twist (Twi), a marker of the presum ...
... additional large field of cells posterior to the pole cells was observed (Fig. 2I). We reasoned that these cells might be mesodermal tissue remaining at the embryo surface as a consequence of invagination failure. To confirm this, we stained tslΔ embryos with anti-Twist (Twi), a marker of the presum ...
The Cellular and Humoral Immune Response against Primary
... is still unclear what role ticks and other arthropods play in the spread of C. burnetii in the environment. History: Q fever was initially described in 1937 by Edward Holbrook Derrick as a febrile illness found in abattoir workers in Brisbane, Queensland, Australia (13). Due to its intracellular na ...
... is still unclear what role ticks and other arthropods play in the spread of C. burnetii in the environment. History: Q fever was initially described in 1937 by Edward Holbrook Derrick as a febrile illness found in abattoir workers in Brisbane, Queensland, Australia (13). Due to its intracellular na ...
A central role for the notochord in vertebral patterning
... nuclei. (C) Representative transverse section through the epiphyseal bar of the skull (arrowheads) at 20 dpf showing large numbers of osteoblasts. (D) Representative transverse section (20 µm) through a mid-trunk centrum at 20 dpf, with no osteoblast labelling in the notochord (n) and surrounding ce ...
... nuclei. (C) Representative transverse section through the epiphyseal bar of the skull (arrowheads) at 20 dpf showing large numbers of osteoblasts. (D) Representative transverse section (20 µm) through a mid-trunk centrum at 20 dpf, with no osteoblast labelling in the notochord (n) and surrounding ce ...
Liver development
... hepatogenesis have been identified. Recently application of this knowledge has enabled researchers to produce “hepatic-like” tissue from embryonic stem (ES) cells in vitro, which may ultimately lead to therapeutically useful tissue for transplantation. This review summarizes the current understandin ...
... hepatogenesis have been identified. Recently application of this knowledge has enabled researchers to produce “hepatic-like” tissue from embryonic stem (ES) cells in vitro, which may ultimately lead to therapeutically useful tissue for transplantation. This review summarizes the current understandin ...
Echinoderm enteric nervous system
... that elongate towards the site of innervation (Cobb, 1969b; Pentreath and Cobb, 1972; Rivera-Bermúdez, 1992; DíazMiranda et al., 1995). In some cases, these processes can be found at a considerable distance from the main cell body. The presence of the visceral plexus in the digestive tract of other ...
... that elongate towards the site of innervation (Cobb, 1969b; Pentreath and Cobb, 1972; Rivera-Bermúdez, 1992; DíazMiranda et al., 1995). In some cases, these processes can be found at a considerable distance from the main cell body. The presence of the visceral plexus in the digestive tract of other ...
- Wiley Online Library
... relation to its point of origin in response to EIX, the less FYVE it contains. This seems to support the notion that different subpopulations of endosomes internalize LeEix2 in response to EIX. The amount of FYVE localized to different endosomes correlates with specific lipid content (Stenmark et al ...
... relation to its point of origin in response to EIX, the less FYVE it contains. This seems to support the notion that different subpopulations of endosomes internalize LeEix2 in response to EIX. The amount of FYVE localized to different endosomes correlates with specific lipid content (Stenmark et al ...
Extracellular matrix of the charophycean green algae
... to especially thank Dr. Michael Gretz for his advice, direction, and consistent encouragement. In fact, I would be remiss if I did not thank the entire Gretz family, including Mrs. Anna Gretz and MicheleGretz, for always treating me like a member of the family and for taking such wonderful care of m ...
... to especially thank Dr. Michael Gretz for his advice, direction, and consistent encouragement. In fact, I would be remiss if I did not thank the entire Gretz family, including Mrs. Anna Gretz and MicheleGretz, for always treating me like a member of the family and for taking such wonderful care of m ...
General microbiology
... the viral proteins only the viral genetic material and associated lipids the viral genetic material and associated proteins Polymerases are encoded on the genomes of: A. all viruses B. all DNA viruses C. *all RNA viruses D. only the largest viruses (ex. Herpesviruses, Paramyxoviruses, Rhabdoviruses, ...
... the viral proteins only the viral genetic material and associated lipids the viral genetic material and associated proteins Polymerases are encoded on the genomes of: A. all viruses B. all DNA viruses C. *all RNA viruses D. only the largest viruses (ex. Herpesviruses, Paramyxoviruses, Rhabdoviruses, ...
Notochord vacuoles are lysosome-related organelles that function in
... trafficking in vacuole formation we asked whether clathrin coats are required. Clathrin coats are important mediators of coated vesicle formation in post-Golgi trafficking as well as during plasma membrane internalization from the cell surface (Anderson et al., 1977; Deborde et al., 2008). The trans ...
... trafficking in vacuole formation we asked whether clathrin coats are required. Clathrin coats are important mediators of coated vesicle formation in post-Golgi trafficking as well as during plasma membrane internalization from the cell surface (Anderson et al., 1977; Deborde et al., 2008). The trans ...
Chloroplast anchoring: its implications for the
... completion of the photo-relocation movement of chloroplasts induced by red light, when protonemata were illuminated with far-red light to convert phytochrome from its far-red-light-absorbing form (Pfr) back to its red-lightabsorbing form (Pr), the induced specific distribution pattern of chloroplast ...
... completion of the photo-relocation movement of chloroplasts induced by red light, when protonemata were illuminated with far-red light to convert phytochrome from its far-red-light-absorbing form (Pfr) back to its red-lightabsorbing form (Pr), the induced specific distribution pattern of chloroplast ...
Notochord vacuoles are lysosome-related organelles that function in
... whereas the inner cells form large fluid-filled intracellular vacuoles. The vacuoles occupy the majority of the volume of the inner cells with diameters of up to 40 µm. Notochord vacuoles have been described in nearly every embryonic vertebrate studied from fish to amphibians, birds, and mammals (Le ...
... whereas the inner cells form large fluid-filled intracellular vacuoles. The vacuoles occupy the majority of the volume of the inner cells with diameters of up to 40 µm. Notochord vacuoles have been described in nearly every embryonic vertebrate studied from fish to amphibians, birds, and mammals (Le ...
Pharmacol Rev.2006;58(2):194-243
... and the possibility of using alternative tissue sources including xenogenic islets and stem cell derived -cell lines. Scientists have made many advances in islet transplantation in recent years. In June 2000, Shapiro et al. at the University of Alberta in Edmonton, Canada, published results of an e ...
... and the possibility of using alternative tissue sources including xenogenic islets and stem cell derived -cell lines. Scientists have made many advances in islet transplantation in recent years. In June 2000, Shapiro et al. at the University of Alberta in Edmonton, Canada, published results of an e ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.