... Two heads are better than one, according to the old expression. So how about tens of heads, all working together to answer scientific questions and translate biomedical discoveries for use in the clinic? A core vision of the TBSI is to encourage collaboration between scientific, engineering and medi ...
University of Zurich - Institute of Molecular Life Sciences
... Probes, Leiden, The Netherlands) was performed as described (96). Ad35-eGFP, Ad5eGFP and Ad2-ts1 were used as described (31, 74, 101). HSV-1-eGFP was a kind gift from Dr. C. Fraefel (Institute of Veterinary Virology, University of Zurich,76). ...
... Probes, Leiden, The Netherlands) was performed as described (96). Ad35-eGFP, Ad5eGFP and Ad2-ts1 were used as described (31, 74, 101). HSV-1-eGFP was a kind gift from Dr. C. Fraefel (Institute of Veterinary Virology, University of Zurich,76). ...
Molecular Plant-Microbe Interactions
... Legumes are unique in the extent of their responses to Nod factor signals. Although considerable progress has been made in understanding the molecular biology, genetics, and biochemistry of the bacterial partner, the molecular mechanisms of nodule organogenesis programmed in the host legume plant ar ...
... Legumes are unique in the extent of their responses to Nod factor signals. Although considerable progress has been made in understanding the molecular biology, genetics, and biochemistry of the bacterial partner, the molecular mechanisms of nodule organogenesis programmed in the host legume plant ar ...
What sort of Science is Glycoscience?
... • Even enveloped viruses that bud from surfaces of infected cells carry with them the glycosylation patterns of the host cell. • Most secreted molecules are glycosylated, and extracellular matrices of multicellular organisms are rich in glycans • Biofilms/ matrices secreted by unicellular organisms ...
... • Even enveloped viruses that bud from surfaces of infected cells carry with them the glycosylation patterns of the host cell. • Most secreted molecules are glycosylated, and extracellular matrices of multicellular organisms are rich in glycans • Biofilms/ matrices secreted by unicellular organisms ...
differentiation and proliferation of embryonic mast
... cell development in rat embryos. Mast cells arise ubiquitously in and are confined to the loose connective tissue in the embryo. The alcian blue--safranin reaction distinguishes between weakly sulfated and strongly sulfated mucopolysaccharides by a shift from alcian blue to safranin staining. Based ...
... cell development in rat embryos. Mast cells arise ubiquitously in and are confined to the loose connective tissue in the embryo. The alcian blue--safranin reaction distinguishes between weakly sulfated and strongly sulfated mucopolysaccharides by a shift from alcian blue to safranin staining. Based ...
Cytoplasmic calcium measurements in intact higher plant cells
... The resting potential across the plasma membrane of root hair cells was between —140 and -160mV in both species. Insertion of microelectrodes caused immediate cessation of cytoplasmic streaming; 1-4 min later streaming restarted and quickly recovered its normal velocity of 1-3/mis" 1 . Once the elec ...
... The resting potential across the plasma membrane of root hair cells was between —140 and -160mV in both species. Insertion of microelectrodes caused immediate cessation of cytoplasmic streaming; 1-4 min later streaming restarted and quickly recovered its normal velocity of 1-3/mis" 1 . Once the elec ...
Measles Virus Matrix Protein Inhibits Host Cell Transcription
... M protein has also been shown to interact with the RNP complex and regulates viral RNA synthesis via its interaction with the N protein [6]. M protein, through its varied interactions, is thus central to MeV assembly. In addition to P protein, the P gene encodes two nonstructural proteins, V and C v ...
... M protein has also been shown to interact with the RNP complex and regulates viral RNA synthesis via its interaction with the N protein [6]. M protein, through its varied interactions, is thus central to MeV assembly. In addition to P protein, the P gene encodes two nonstructural proteins, V and C v ...
A Comparison of the Effects of DNA
... following transfer to a different plant system, promoters from bean CHS, Arabidopsis PAL, and pea DRR206 genes were evaluated in tobacco. Tobacco plants used had been transformed with DNA constructs containing promoters from a representative of each of these defense genes and the 35S promoter linked ...
... following transfer to a different plant system, promoters from bean CHS, Arabidopsis PAL, and pea DRR206 genes were evaluated in tobacco. Tobacco plants used had been transformed with DNA constructs containing promoters from a representative of each of these defense genes and the 35S promoter linked ...
Slide 1
... that observed between P. aeruginosa and C. albicans (3). The antagonistic effects were abrogated in the context of a mixed-species biofilm. A robust biofilm composed of all five organisms, recapitulating the original explant biofilm, was established over the course of six weeks. To test our hypothes ...
... that observed between P. aeruginosa and C. albicans (3). The antagonistic effects were abrogated in the context of a mixed-species biofilm. A robust biofilm composed of all five organisms, recapitulating the original explant biofilm, was established over the course of six weeks. To test our hypothes ...
A Geminivirus lnduces Expression of a Host DNA Synthesis Protein
... animal DNA polymerases a (Pola) and p have been identified in plant nuclei and are thought to be involved in replication and repair, respectively. There is no direct evidence for the involvement of the plant Pola-likeenzyme in nuclear DNA replication, but its activity was found to increase 100-fold ...
... animal DNA polymerases a (Pola) and p have been identified in plant nuclei and are thought to be involved in replication and repair, respectively. There is no direct evidence for the involvement of the plant Pola-likeenzyme in nuclear DNA replication, but its activity was found to increase 100-fold ...
Immunocytochemical Localization of the
... included in many therapeutic regimens for the treatment of various forms of leukemias and lymphomas (1,2). These actions may be both direct on the malignant cell and indirect, through the down-regulation of lymphokines upon which some, but not all such malignancies depend (3). The exact cause of the ...
... included in many therapeutic regimens for the treatment of various forms of leukemias and lymphomas (1,2). These actions may be both direct on the malignant cell and indirect, through the down-regulation of lymphokines upon which some, but not all such malignancies depend (3). The exact cause of the ...
lesson 1: plant cells
... 1) Plants use this source of energy for photosynthesis 2) When plant cells divide in half it is called __________. 3) Humans can’t make their own sugar; we have to ___. 4) Glucose is a type of _____. 5) This is produced by plants during photosynthesis. It is also used by plants during respiration. 6 ...
... 1) Plants use this source of energy for photosynthesis 2) When plant cells divide in half it is called __________. 3) Humans can’t make their own sugar; we have to ___. 4) Glucose is a type of _____. 5) This is produced by plants during photosynthesis. It is also used by plants during respiration. 6 ...
View PDF
... example). In the skin, the different roles of senescent cells in promoting both physiological and pathophysiological conditions are still in early phases of discovery. More experiments need to be done to determine how senescent cells can tip the balance between efficient and chronic wound healing. I ...
... example). In the skin, the different roles of senescent cells in promoting both physiological and pathophysiological conditions are still in early phases of discovery. More experiments need to be done to determine how senescent cells can tip the balance between efficient and chronic wound healing. I ...
The major salivary glands
... ducts are lined by low cuboidal epithelial cells • Striated duct, so-called because of the presence of “striations,” the infoldings of the basal plasma membrane of the columnar cells that form the duct. Striated ducts are lined by a simple cuboidal epithelium that gradually becomes columnar as it ap ...
... ducts are lined by low cuboidal epithelial cells • Striated duct, so-called because of the presence of “striations,” the infoldings of the basal plasma membrane of the columnar cells that form the duct. Striated ducts are lined by a simple cuboidal epithelium that gradually becomes columnar as it ap ...
Tumor-specific T cells in human Merkel cell carcinomas: a possible
... TCR repertoire among both CD4+ and CD8+ T cells, suggesting expansion may be antigenspecific (Figure 6a-c). The expansion of T cells and simultaneous loss of viable tumor cells suggested that viable tumor cells may be killed by expanding T cells (Figure 6d). Cytokine treated CD8+ TILs showed upregul ...
... TCR repertoire among both CD4+ and CD8+ T cells, suggesting expansion may be antigenspecific (Figure 6a-c). The expansion of T cells and simultaneous loss of viable tumor cells suggested that viable tumor cells may be killed by expanding T cells (Figure 6d). Cytokine treated CD8+ TILs showed upregul ...
Antifolate Polyglutamylation and Competitive Drug Displacement at
... Previous studies from this laboratory have shown that the addition of leucovorin to tumor cells dissociates methotrexate, but not methotrexate polyglutamates, from dihydrofolate reduc Ã-ase(L. H. Matherly, D. W. Fry, and I. D. Goldman, Cancer Res., 43: 2694-2699, 1983). To further assess the importa ...
... Previous studies from this laboratory have shown that the addition of leucovorin to tumor cells dissociates methotrexate, but not methotrexate polyglutamates, from dihydrofolate reduc Ã-ase(L. H. Matherly, D. W. Fry, and I. D. Goldman, Cancer Res., 43: 2694-2699, 1983). To further assess the importa ...
The Journal of Experimental Medicine
... of siRNA molecules, cells were washed twice with serum-free RPMI 1640 medium. Cells were transfected with 600 nM control (siK3; specific for nucleotides 2610–2630 of neomycin phosphotransferase mRNA; sequence data are available from GenBank/EMBL/DDBJ under accession no. U55763) or c-FLIPS/L– specifi ...
... of siRNA molecules, cells were washed twice with serum-free RPMI 1640 medium. Cells were transfected with 600 nM control (siK3; specific for nucleotides 2610–2630 of neomycin phosphotransferase mRNA; sequence data are available from GenBank/EMBL/DDBJ under accession no. U55763) or c-FLIPS/L– specifi ...
Chapter 2 Role of the synthase domain of Ags1p in cell wall α
... Distinct plasma membrane-localised synthases are responsible for the production of structural polysaccharides in the fungal cell wall, mostly (1,3)-β-glucan, chitin, and α-glucan. (1,3)-β-Glucan and chitin synthases were identified first in the budding yeast Saccharomyces cerevisiae (Douglas et al., 1 ...
... Distinct plasma membrane-localised synthases are responsible for the production of structural polysaccharides in the fungal cell wall, mostly (1,3)-β-glucan, chitin, and α-glucan. (1,3)-β-Glucan and chitin synthases were identified first in the budding yeast Saccharomyces cerevisiae (Douglas et al., 1 ...
PDF - Eleanor Maine Research Lab
... Stem cells achieve self-renewal through both the execution of mitotic cell division and maintenance of stem cell fate. Different stem cell types display distinct patterns of self-renewal and differentiation. For example, hematopoetic stem cells divide infrequently compared with ES cells (Orford and ...
... Stem cells achieve self-renewal through both the execution of mitotic cell division and maintenance of stem cell fate. Different stem cell types display distinct patterns of self-renewal and differentiation. For example, hematopoetic stem cells divide infrequently compared with ES cells (Orford and ...
Polarization of Thyroid Cells in Culture
... and II). Iodide equilibrium between cells and medium was reached after 30-min incubation at 37°C (result not shown). Perchlorate (1 mM) abolished halogen concentration. Based on the assumption that, in the presence of perchlorate, the ratio of iodide concentration between cells and medium (C/M) is 1 ...
... and II). Iodide equilibrium between cells and medium was reached after 30-min incubation at 37°C (result not shown). Perchlorate (1 mM) abolished halogen concentration. Based on the assumption that, in the presence of perchlorate, the ratio of iodide concentration between cells and medium (C/M) is 1 ...
Agrobacterium tumefaciens- mediated Transformation of Plant Cells
... and VirE2, which interact with the endogenous nuclear localization machinery. Once in the nucleus, the DNA integrates stably into the plant genome in an as yet poorly characterized process that is likely to include the activities of both vir gene products and host enzymes. The integration site appea ...
... and VirE2, which interact with the endogenous nuclear localization machinery. Once in the nucleus, the DNA integrates stably into the plant genome in an as yet poorly characterized process that is likely to include the activities of both vir gene products and host enzymes. The integration site appea ...
Nature - Andreas Jenny`s Laboratory
... be to counteract the morphogenetic consequences of mitosis, which results in loss of polarity and the exclusion of apical daughter cells from the neuroepithelium. The strictest inference of this model would be that MZtri neurulation defects may be suppressed by blocking cell division, thus precludin ...
... be to counteract the morphogenetic consequences of mitosis, which results in loss of polarity and the exclusion of apical daughter cells from the neuroepithelium. The strictest inference of this model would be that MZtri neurulation defects may be suppressed by blocking cell division, thus precludin ...
AMP-activated protein kinase induces apoptosis in LX2 cell
... Recent evidence has indicated that AMPactivated protein kinase (AMPK) can induce apoptosis of several kinds of cells, such as rat liver cells, MIN6 cells and human neuroblastoma cells, but little is known regarding this matter in HSCs. ...
... Recent evidence has indicated that AMPactivated protein kinase (AMPK) can induce apoptosis of several kinds of cells, such as rat liver cells, MIN6 cells and human neuroblastoma cells, but little is known regarding this matter in HSCs. ...
Myc Requires Distinct E2F Activities to Induce S Phase
... that encode proteins involved in DNA replication, such as ornithine decarboxylase (Wagner et al., 1993; BelloFernandez et al., 1993), proteins that function in the G1 transition, such as Cul1, cdk4, and the Cdc25A phosphatase (O’Hagan et al., 2000; Hermeking et al., 2000; Galaktionov et al., 1996), ...
... that encode proteins involved in DNA replication, such as ornithine decarboxylase (Wagner et al., 1993; BelloFernandez et al., 1993), proteins that function in the G1 transition, such as Cul1, cdk4, and the Cdc25A phosphatase (O’Hagan et al., 2000; Hermeking et al., 2000; Galaktionov et al., 1996), ...
Developmental control of blood cell migration by the Drosophila VEGF pathway. Cell 108, 865-876. pdf
... Blood cells (hemocytes) in Drosophila also migrate extensively during development (Tepass et al., 1994). They originate in the head mesoderm, and over a 7 hr period in midembryogenesis they migrate along specific pathways to disperse throughout the body, where they function as immune and interstitia ...
... Blood cells (hemocytes) in Drosophila also migrate extensively during development (Tepass et al., 1994). They originate in the head mesoderm, and over a 7 hr period in midembryogenesis they migrate along specific pathways to disperse throughout the body, where they function as immune and interstitia ...
Cellular differentiation
In developmental biology, cellular differentiation isa cell changes from one cell type to another. Most commonly this is a less specialized type becoming a more specialized type, such as during cell growth. Differentiation occurs numerous times during the development of a multicellular organism as it changes from a simple zygote to a complex system of tissues and cell types. Differentiation continues in adulthood as adult stem cells divide and create fully differentiated daughter cells during tissue repair and during normal cell turnover. Some differentiation occurs in response to antigen exposure. Differentiation dramatically changes a cell's size, shape, membrane potential, metabolic activity, and responsiveness to signals. These changes are largely due to highly controlled modifications in gene expression and are the study of epigenetics. With a few exceptions, cellular differentiation almost never involves a change in the DNA sequence itself. Thus, different cells can have very different physical characteristics despite having the same genome.A cell that can differentiate into all cell types of the adult organism is known as pluripotent. Such cells are called embryonic stem cells in animals and meristematic cells in higher plants. A cell that can differentiate into all cell types, including the placental tissue, is known as totipotent. In mammals, only the zygote and subsequent blastomeres are totipotent, while in plants many differentiated cells can become totipotent with simple laboratory techniques. In cytopathology, the level of cellular differentiation is used as a measure of cancer progression. ""Grade"" is a marker of how differentiated a cell in a tumor is.