Changes in Cell Morphology and the Cellular Localization of Protein
... used had a temperature sensitive mutation in cdc2, a protein he had shown was required for the fission yeast to progress through the cell cycle. What he found was that despite all the differences between fission yeast and humans, there was indeed a human gene that could replace the function of mutan ...
... used had a temperature sensitive mutation in cdc2, a protein he had shown was required for the fission yeast to progress through the cell cycle. What he found was that despite all the differences between fission yeast and humans, there was indeed a human gene that could replace the function of mutan ...
Myc Requires Distinct E2F Activities to Induce S Phase
... that encode proteins involved in DNA replication, such as ornithine decarboxylase (Wagner et al., 1993; BelloFernandez et al., 1993), proteins that function in the G1 transition, such as Cul1, cdk4, and the Cdc25A phosphatase (O’Hagan et al., 2000; Hermeking et al., 2000; Galaktionov et al., 1996), ...
... that encode proteins involved in DNA replication, such as ornithine decarboxylase (Wagner et al., 1993; BelloFernandez et al., 1993), proteins that function in the G1 transition, such as Cul1, cdk4, and the Cdc25A phosphatase (O’Hagan et al., 2000; Hermeking et al., 2000; Galaktionov et al., 1996), ...
Biology of the Cell
... closer than a few hundred nanometers, the light beams reflecting from the two images start to overlap. The only way two light beams can get closer together and still be resolved is if their “wavelengths” are shorter. One way to avoid overlap is by using a beam of electrons rather than a beam of ligh ...
... closer than a few hundred nanometers, the light beams reflecting from the two images start to overlap. The only way two light beams can get closer together and still be resolved is if their “wavelengths” are shorter. One way to avoid overlap is by using a beam of electrons rather than a beam of ligh ...
Clone
... GATA-3 (GATA binding protein 3) is a member of the GATA family of transcription factors. This 50 kD anuclear protein regulates the development and subsequent maintenance of a variety of human tissues, including hematopoietic cells, skin, kidney, mammary gland, and the central nervous system. Among s ...
... GATA-3 (GATA binding protein 3) is a member of the GATA family of transcription factors. This 50 kD anuclear protein regulates the development and subsequent maintenance of a variety of human tissues, including hematopoietic cells, skin, kidney, mammary gland, and the central nervous system. Among s ...
Characterisation of dendritic cell subsets in lung cancer micro-environments A. Bergeron*
... these therapeutic approaches was generally limited [3]. Thus, questions have been raised concerning the type and activation state of human DCs that should be used to induce an effective antitumoural immunity. ...
... these therapeutic approaches was generally limited [3]. Thus, questions have been raised concerning the type and activation state of human DCs that should be used to induce an effective antitumoural immunity. ...
KOBITO1 Encodes a Novel Plasma Membrane Protein Necessary
... show increased pectin content, as shown by the higher galacturonic acid levels compared with the wild-type control. (B) and (C) Matrix-assisted laser desorption ionization–time of flight mass spectra of the fragments produced from wild-type (B) and kob1-1 (C) cell walls. No significant differences i ...
... show increased pectin content, as shown by the higher galacturonic acid levels compared with the wild-type control. (B) and (C) Matrix-assisted laser desorption ionization–time of flight mass spectra of the fragments produced from wild-type (B) and kob1-1 (C) cell walls. No significant differences i ...
co-culture of hypothalamic neurons and melanotrope cells
... France) at day 15 of gestation (day 0 of gestation corresponding to the day of fecondation) were anesthetized with pentobarbital and the embryos removed. The diencephalic area corresponding to the hypothalamus was isolated and incubated with trypsin–EDTA (0.05–0.02%; Gibco) for 7 min at 37)C under c ...
... France) at day 15 of gestation (day 0 of gestation corresponding to the day of fecondation) were anesthetized with pentobarbital and the embryos removed. The diencephalic area corresponding to the hypothalamus was isolated and incubated with trypsin–EDTA (0.05–0.02%; Gibco) for 7 min at 37)C under c ...
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... reports of single cases or small series of patient with BCCs on sites other than the scrotum, a significant relationship between HPV and BCCs in the general population has yet to be established. ...
... reports of single cases or small series of patient with BCCs on sites other than the scrotum, a significant relationship between HPV and BCCs in the general population has yet to be established. ...
pcDNA™3.1(+) - Thermo Fisher Scientific
... To successfully generate a stable cell line expressing your protein of interest, you need to determine the minimum concentration of Geneticin® required to kill your untransfected host cell line. Test a range of concentrations (see protocol below) to ensure that you determine the minimum concentratio ...
... To successfully generate a stable cell line expressing your protein of interest, you need to determine the minimum concentration of Geneticin® required to kill your untransfected host cell line. Test a range of concentrations (see protocol below) to ensure that you determine the minimum concentratio ...
Cell Biology - New Age International
... glycolipids) and (ii) most of the proteins exposed at outer surface (forming glycoproteins). The carbohydrates so bound to membrane components constitute the glycocalyx of cell surface. 7. The sugar portions of glycolipids and glycoproteins are involved in recognition mechanisms:(a) Sugar recognitio ...
... glycolipids) and (ii) most of the proteins exposed at outer surface (forming glycoproteins). The carbohydrates so bound to membrane components constitute the glycocalyx of cell surface. 7. The sugar portions of glycolipids and glycoproteins are involved in recognition mechanisms:(a) Sugar recognitio ...
The Plant Secretory Pathway: An Essential
... cell wall materials have been generating valuable information for diverse practical applications. Nonetheless, the identity of the endomembrane components necessary for the transport of cell wall enzymes and polysaccharides is not well known. Evidence indicates that plant cells can accomplish secret ...
... cell wall materials have been generating valuable information for diverse practical applications. Nonetheless, the identity of the endomembrane components necessary for the transport of cell wall enzymes and polysaccharides is not well known. Evidence indicates that plant cells can accomplish secret ...
PDF - Oxford Academic - Oxford University Press
... explain why most algae have thin body plans with large surface areas. The existence of these two types of parameters is important because it shows that evolution is constrained by physical laws, but that the effects of these laws can be modified by biological innovation. Given the huge scope of the ...
... explain why most algae have thin body plans with large surface areas. The existence of these two types of parameters is important because it shows that evolution is constrained by physical laws, but that the effects of these laws can be modified by biological innovation. Given the huge scope of the ...
The plasma membrane recycling pathway and cell polarity in plants
... hormone auxin are the basis of differential cell behaviour during pattern formation in higher plants (Benková et al., 2003; Friml et al., 2003; Reinhardt et al., 2003). Auxin distribution throughout the whole plant is controlled by at least two families of plasma-membrane associated proteins, called ...
... hormone auxin are the basis of differential cell behaviour during pattern formation in higher plants (Benková et al., 2003; Friml et al., 2003; Reinhardt et al., 2003). Auxin distribution throughout the whole plant is controlled by at least two families of plasma-membrane associated proteins, called ...
A Sugar-Inducible Protein Kinase, VvSK1, Regulates Hexose
... Sugars provide the reduced carbon needed for the structural materials of the cell, storage compounds, and all major primary and secondary metabolites. They also influence plant growth and development by acting as regulatory signals, which affect the expression of various genes involved in many proce ...
... Sugars provide the reduced carbon needed for the structural materials of the cell, storage compounds, and all major primary and secondary metabolites. They also influence plant growth and development by acting as regulatory signals, which affect the expression of various genes involved in many proce ...
Nuclear pore interactions with the genome
... interact with the NPC, each having different molecular requirements and different outputs (Figure 1). Salt stress primes many genes for faster activation in response to H2O2 treatment. This effect persists for 3– 4 generations and requires Nup42; in nup42D mutants, the rate of activation of these ge ...
... interact with the NPC, each having different molecular requirements and different outputs (Figure 1). Salt stress primes many genes for faster activation in response to H2O2 treatment. This effect persists for 3– 4 generations and requires Nup42; in nup42D mutants, the rate of activation of these ge ...
Lec-2 Cell Structure
... 1. Transport. (left) A protein that spans the membrane may provide a hydrophilic channel across the membrane that is selective for a particular solute. (right) Other transport proteins shuttle a substance from one side to the other by changing shape. Some of these proteins hydrolyze ATP as an energy ...
... 1. Transport. (left) A protein that spans the membrane may provide a hydrophilic channel across the membrane that is selective for a particular solute. (right) Other transport proteins shuttle a substance from one side to the other by changing shape. Some of these proteins hydrolyze ATP as an energy ...
2.6 Isolation of trout head kidney and spleen leukocytes
... lymphoid tissue), heart, brain, muscle, spleen, liver and head kidney, were removed aseptically and placed into 1 ml of TRI Reagent® (Sigma–Aldrich) for RNA preparation and cDNA synthesis. The cDNA samples (20 µl after cDNA production) were diluted with 80 µl of TE buffer (10 mM Tris, 1 mM EDTA, pH ...
... lymphoid tissue), heart, brain, muscle, spleen, liver and head kidney, were removed aseptically and placed into 1 ml of TRI Reagent® (Sigma–Aldrich) for RNA preparation and cDNA synthesis. The cDNA samples (20 µl after cDNA production) were diluted with 80 µl of TE buffer (10 mM Tris, 1 mM EDTA, pH ...
2. Summary Final Thesis
... The main goal of this thesis was to study the changes in the proteome of Molm-13 cells from Acute Myeloid Leukemia when treated with a proliferation and cell differentiation inductor (FLT3 ligand) and with an apoptosis inductor (Okadaic Acid, OA, FLT3 ligand binds to FLT3 receptor which auto phospho ...
... The main goal of this thesis was to study the changes in the proteome of Molm-13 cells from Acute Myeloid Leukemia when treated with a proliferation and cell differentiation inductor (FLT3 ligand) and with an apoptosis inductor (Okadaic Acid, OA, FLT3 ligand binds to FLT3 receptor which auto phospho ...
IP3R-mediated Ca2+ release regulates protein metabolism in
... (Fig. S2A), its pupariation was sensitive to reduction of yeast, the major source of dietary protein in ‘normal food’ (Fig. 2A). Pupariation was also reduced on sucrose (Fig. S2A), and rescued by supplementation with amino acids (Jayakumar et al., 2016) or amino acids and vitamins, but not lipids or ...
... (Fig. S2A), its pupariation was sensitive to reduction of yeast, the major source of dietary protein in ‘normal food’ (Fig. 2A). Pupariation was also reduced on sucrose (Fig. S2A), and rescued by supplementation with amino acids (Jayakumar et al., 2016) or amino acids and vitamins, but not lipids or ...
Dentistry_-_Test_questions_2016
... a. most antigens induce a response from more than one clone of lymphocytes b. a large protein antigen generally can combine with many different antibody molecules c. a hapten can stimulate antibody production but cannot combine with antibody molecules. d. MHC genes play a major role in determining t ...
... a. most antigens induce a response from more than one clone of lymphocytes b. a large protein antigen generally can combine with many different antibody molecules c. a hapten can stimulate antibody production but cannot combine with antibody molecules. d. MHC genes play a major role in determining t ...
Chapter 4 Notes
... • The structures and organelles of eukaryotic cells perform four basic functions. 1. The nucleus and ribosomes are involved in the genetic control of the cell. 2. The endoplasmic reticulum, Golgi apparatus, lysosomes, vacuoles, and peroxisomes are involved in the manufacture, distribution, and break ...
... • The structures and organelles of eukaryotic cells perform four basic functions. 1. The nucleus and ribosomes are involved in the genetic control of the cell. 2. The endoplasmic reticulum, Golgi apparatus, lysosomes, vacuoles, and peroxisomes are involved in the manufacture, distribution, and break ...
Cytoskeletal rearrangements in gastric epithelial cells in response to
... Herein, we observed similar effects with both type I and type II H. pylori strains, thereby confirming the work of Palovuori et al. (2000). Cell morphology changes were observed not only using AGS cells, but also in other epithelial cell types, including HEp-2 cells and T84 cells that have previousl ...
... Herein, we observed similar effects with both type I and type II H. pylori strains, thereby confirming the work of Palovuori et al. (2000). Cell morphology changes were observed not only using AGS cells, but also in other epithelial cell types, including HEp-2 cells and T84 cells that have previousl ...
The Role of Endocytosis in the Creation of the Cortical Division Zone
... when the cells enter prometaphase. However, some positional information, or positional memory, is retained in the cell cortex/plasma membrane where the PPB of MTs was located, and the cell plate edge grows towards and fuses with this predetermined division site. Thus, how MTs demarcate the future di ...
... when the cells enter prometaphase. However, some positional information, or positional memory, is retained in the cell cortex/plasma membrane where the PPB of MTs was located, and the cell plate edge grows towards and fuses with this predetermined division site. Thus, how MTs demarcate the future di ...
Direct conversion of root primordium into shoot meristem relies on
... callus-inducing medium (CIM) characterized by a high auxin/ cytokinin ratio. After a few days, the resulting calli can be transferred on a root-inducing medium (RIM) that only contains auxin or on a shoot-inducing medium (SIM) with a high cytokinin/ auxin ratio, with founder cells then initiating th ...
... callus-inducing medium (CIM) characterized by a high auxin/ cytokinin ratio. After a few days, the resulting calli can be transferred on a root-inducing medium (RIM) that only contains auxin or on a shoot-inducing medium (SIM) with a high cytokinin/ auxin ratio, with founder cells then initiating th ...
Embryonic Stem Cell Culture Conditions Support Distinct States
... participate in development and generate all cell types of the embryo including the germ cells (Beddington and Robertson, 1989; Gossler et al., 1986; Lallemand and Brulet, 1990; Robertson et al., 1986; Suemori et al., 1990). They are therefore said to be pluripotent. Although the first ESCs were deri ...
... participate in development and generate all cell types of the embryo including the germ cells (Beddington and Robertson, 1989; Gossler et al., 1986; Lallemand and Brulet, 1990; Robertson et al., 1986; Suemori et al., 1990). They are therefore said to be pluripotent. Although the first ESCs were deri ...
Cellular differentiation
In developmental biology, cellular differentiation isa cell changes from one cell type to another. Most commonly this is a less specialized type becoming a more specialized type, such as during cell growth. Differentiation occurs numerous times during the development of a multicellular organism as it changes from a simple zygote to a complex system of tissues and cell types. Differentiation continues in adulthood as adult stem cells divide and create fully differentiated daughter cells during tissue repair and during normal cell turnover. Some differentiation occurs in response to antigen exposure. Differentiation dramatically changes a cell's size, shape, membrane potential, metabolic activity, and responsiveness to signals. These changes are largely due to highly controlled modifications in gene expression and are the study of epigenetics. With a few exceptions, cellular differentiation almost never involves a change in the DNA sequence itself. Thus, different cells can have very different physical characteristics despite having the same genome.A cell that can differentiate into all cell types of the adult organism is known as pluripotent. Such cells are called embryonic stem cells in animals and meristematic cells in higher plants. A cell that can differentiate into all cell types, including the placental tissue, is known as totipotent. In mammals, only the zygote and subsequent blastomeres are totipotent, while in plants many differentiated cells can become totipotent with simple laboratory techniques. In cytopathology, the level of cellular differentiation is used as a measure of cancer progression. ""Grade"" is a marker of how differentiated a cell in a tumor is.