Andrei Fagarasanu Department of Cell Biology Supervisor: Dr
... and George Palade, who is considered the father of modern cell biology. While a medical student, I participated as a teaching assistant in anatomy. This opportunity developed in me a strong interest for basic research and the molecular mechanisms underlying diseases. In 2002 I came to Canada to be p ...
... and George Palade, who is considered the father of modern cell biology. While a medical student, I participated as a teaching assistant in anatomy. This opportunity developed in me a strong interest for basic research and the molecular mechanisms underlying diseases. In 2002 I came to Canada to be p ...
CDK11p58 kinase activity is required to protect sister chromatid
... assessed whether CDK11 depletion could affect the Bub1/H2A-T120/Sgo1 pathway. CDK11depleted cells were labeled with anti-Bub1, anti-Sgo1 and anti-H2AT120P antibodies. Human CREST autoimmune serum was used as a centromere marker and centromere signal intensities for the different labelings were quant ...
... assessed whether CDK11 depletion could affect the Bub1/H2A-T120/Sgo1 pathway. CDK11depleted cells were labeled with anti-Bub1, anti-Sgo1 and anti-H2AT120P antibodies. Human CREST autoimmune serum was used as a centromere marker and centromere signal intensities for the different labelings were quant ...
Kainate Activation of Horizontal, Bipolar, Amacrine, and Ganglion
... are OFF-center cone bipolar cells, which exhibit strong heterogeneous responses to KA, as evidenced by their broad AGB histogram. Multiple morphologic subtypes of mammalian ON-center and OFF-center cone bipolar cells exist (Famiglietti, 1981; Wässle and Boycott, 1991; Mills and Massey, 1992; Euler ...
... are OFF-center cone bipolar cells, which exhibit strong heterogeneous responses to KA, as evidenced by their broad AGB histogram. Multiple morphologic subtypes of mammalian ON-center and OFF-center cone bipolar cells exist (Famiglietti, 1981; Wässle and Boycott, 1991; Mills and Massey, 1992; Euler ...
Progressive lineage analysis by cell sorting and culture identifies
... To examine whether this panel of mAbs can define cells at intermediate differentiation stages, we dissociated cells of 7.47.8 dpc embryos and analyzed them by flow cytometry. As shown in Fig. 1A, most E-cadherin− cells expressed either FLK-1 or PDGFRα, or both, confirming that the mesoderm different ...
... To examine whether this panel of mAbs can define cells at intermediate differentiation stages, we dissociated cells of 7.47.8 dpc embryos and analyzed them by flow cytometry. As shown in Fig. 1A, most E-cadherin− cells expressed either FLK-1 or PDGFRα, or both, confirming that the mesoderm different ...
Three-Dimensional Reconstruction of Tubular Structure of Vacuolar
... of the intracellular volume of most higher plant cells, and over 90% of the cell volume in mature tissues. The space-filling properties and solutes of vacuoles therefore play an important role in the growth and morphogenesis of higher plants. During the rapid growth of higher plants, for example, in ...
... of the intracellular volume of most higher plant cells, and over 90% of the cell volume in mature tissues. The space-filling properties and solutes of vacuoles therefore play an important role in the growth and morphogenesis of higher plants. During the rapid growth of higher plants, for example, in ...
Evolutionary tradeoffs in cellular composition across
... The ISME Journal (2016) 10, 2145–2157; doi:10.1038/ismej.2016.21; published online 5 April 2016 ...
... The ISME Journal (2016) 10, 2145–2157; doi:10.1038/ismej.2016.21; published online 5 April 2016 ...
Mesophyll structure during leaf development in Ballota acetabulosa
... cell number per unit surface (Fig. 4) as well as the cells of Ballota, the chloroplasts are arranged just increase of their diameter from L2 to L5 (Fig. 4) - beneath those parts of cell walls exposed to the by c. 14 ''o for spong>' cells and 17 "'„ for palisade cells internal leaf atmosphere and the ...
... cell number per unit surface (Fig. 4) as well as the cells of Ballota, the chloroplasts are arranged just increase of their diameter from L2 to L5 (Fig. 4) - beneath those parts of cell walls exposed to the by c. 14 ''o for spong>' cells and 17 "'„ for palisade cells internal leaf atmosphere and the ...
KS4 Microbes
... People who lead very unhealthy lifestyles sometimes become ill but it is also clear that people can become ill despite leading a healthy lifestyle. Why? ...
... People who lead very unhealthy lifestyles sometimes become ill but it is also clear that people can become ill despite leading a healthy lifestyle. Why? ...
Biology 13100 (by Ken Robinson, revised 2009 by NPelaez) The
... A variety of units are used to report pressure. As a practical matter, it is important to be able to deal with this lack of standardization. Pressure is defined as a force per unit area. The proper international unit for pressure is the pascal (Pa), which is a newton/m2. A newton is the MKS unit of ...
... A variety of units are used to report pressure. As a practical matter, it is important to be able to deal with this lack of standardization. Pressure is defined as a force per unit area. The proper international unit for pressure is the pascal (Pa), which is a newton/m2. A newton is the MKS unit of ...
Regulation of Microtubule Stability and Mitotic
... Members of the IAP4 gene family have been implicated in two cellular functions, suppression of apoptotic cell death and regulation of cell division. Whereas several mammalian IAP proteins counteract apoptosis by interfering with processing and catalytic activity of caspases (1), IAP proteins in yeas ...
... Members of the IAP4 gene family have been implicated in two cellular functions, suppression of apoptotic cell death and regulation of cell division. Whereas several mammalian IAP proteins counteract apoptosis by interfering with processing and catalytic activity of caspases (1), IAP proteins in yeas ...
Somite-derived cells replace ventral aortic
... splanchnopleura-derived ECs (Pardanaud et al., 1996). As only splanchnopleura-born ECs display hemogenic capacities, intraaortic clusters are restricted to the ventral aspect of the aorta. Blood vessels are typically formed by one layer of ECs, one of pericytes and one or several layers of smooth mu ...
... splanchnopleura-derived ECs (Pardanaud et al., 1996). As only splanchnopleura-born ECs display hemogenic capacities, intraaortic clusters are restricted to the ventral aspect of the aorta. Blood vessels are typically formed by one layer of ECs, one of pericytes and one or several layers of smooth mu ...
Achieving CNS axon regeneration by manipulating convergent
... Integrins are transmembrane heterodimeric receptors that facilitate bidirectional signaling between cells and the extracellular environment, most notably proteins found in the ECM or on other cells (e.g., laminin, fibronectin). Integrin binding activates intracellular signaling cascades that affect ...
... Integrins are transmembrane heterodimeric receptors that facilitate bidirectional signaling between cells and the extracellular environment, most notably proteins found in the ECM or on other cells (e.g., laminin, fibronectin). Integrin binding activates intracellular signaling cascades that affect ...
Inhibition of Agrobacterium tumefaciens Growth by Silver Nitrate
... lag phases followed by growth were observed. This is probably due to the adaptation of the microorganism to the chemical with the help of a selective mutation (Alsheikh et al. 2002). AgNO3 strongly influences bacterial viability under a low level of initial cell density and short-term exposure but n ...
... lag phases followed by growth were observed. This is probably due to the adaptation of the microorganism to the chemical with the help of a selective mutation (Alsheikh et al. 2002). AgNO3 strongly influences bacterial viability under a low level of initial cell density and short-term exposure but n ...
A High Throughput, Whole Cell Screen for Small
... long term activation of the spindle checkpoint, whereupon cells arrest at the preanaphase stage for many hours. Many cancers show abnormal chromosome content, often being hyperdiploid. Certain lines derived from tumors have abnormal but relatively stable chromosome content, whereas others exhibit su ...
... long term activation of the spindle checkpoint, whereupon cells arrest at the preanaphase stage for many hours. Many cancers show abnormal chromosome content, often being hyperdiploid. Certain lines derived from tumors have abnormal but relatively stable chromosome content, whereas others exhibit su ...
HAUSP, a deubiquitinating enzyme for p53, is polyubiquitinated
... degradation pathway. According to this result, the level of ubiquitinated rHAUSP was increased with the addition of MG132 (Fig. 3A, lanes 3 and 6). Our results also suggest that rHAUSP is participated in the protein degradation pathway mediated by the ubiquitin-like molecule, nedd8, due to the incre ...
... degradation pathway. According to this result, the level of ubiquitinated rHAUSP was increased with the addition of MG132 (Fig. 3A, lanes 3 and 6). Our results also suggest that rHAUSP is participated in the protein degradation pathway mediated by the ubiquitin-like molecule, nedd8, due to the incre ...
Differential roles for the low-affinity phosphate transporters Pho87
... Pho4 activity and Spl2. Furthermore, when cells are starved of other essential nutrients or are treated with rapamycin, the vacuolar targeting applies to both low-affinity Pi transporters, and this process is also independent of Pho4 and Spl2. Nonetheless, the vacuolar targeting of both Pho87 and Ph ...
... Pho4 activity and Spl2. Furthermore, when cells are starved of other essential nutrients or are treated with rapamycin, the vacuolar targeting applies to both low-affinity Pi transporters, and this process is also independent of Pho4 and Spl2. Nonetheless, the vacuolar targeting of both Pho87 and Ph ...
REPRODUCTION: THE CONTINUITY OF LIFE
... round structure at the tip of a stalk is called a spore case which contains the spores. When the spore case opens, the tiny spores are released and may be carried by wind or water. Once the spore lands on a favourable environment, it develops into a new organism. Under the microscope, a bread mold w ...
... round structure at the tip of a stalk is called a spore case which contains the spores. When the spore case opens, the tiny spores are released and may be carried by wind or water. Once the spore lands on a favourable environment, it develops into a new organism. Under the microscope, a bread mold w ...
Effects of dietary components on Tight junctions (TJ) Lauric acid
... intestinal barrier. TJ are complex protein structures comprised of transmembrane proteins, which interact with the actin cytoskeleton via plaque proteins. Signaling pathways involved in the assembly, disassembly, and maintenance of TJ are controlled by a number of signaling molecules, such as protei ...
... intestinal barrier. TJ are complex protein structures comprised of transmembrane proteins, which interact with the actin cytoskeleton via plaque proteins. Signaling pathways involved in the assembly, disassembly, and maintenance of TJ are controlled by a number of signaling molecules, such as protei ...
Comparative immunolocalization of the plasma membrane calcium
... Na+/(Ca2+ + K+) exchanger and, at the same time, maintains intra-conal Ca2+ at non-toxic concentrations. In calbindin-null mutant mouse, calbindin immunoreactivity was completely absent but those cells that express the protein in wildtype retinas, such as horizontal cells, were still present and app ...
... Na+/(Ca2+ + K+) exchanger and, at the same time, maintains intra-conal Ca2+ at non-toxic concentrations. In calbindin-null mutant mouse, calbindin immunoreactivity was completely absent but those cells that express the protein in wildtype retinas, such as horizontal cells, were still present and app ...
Retinal pigment epithelial cells phagocytosis of T lymphocytes
... also to phagocyte latex beads, red blood cells, algae, yeasts, and bacteria.18 However, to our knowledge, nothing was known about their potential to phagocytose T cells. Here we demonstrated, by three different methods, that RPE cells phagocytose T lymphocytes, in a time dependent manner. In additio ...
... also to phagocyte latex beads, red blood cells, algae, yeasts, and bacteria.18 However, to our knowledge, nothing was known about their potential to phagocytose T cells. Here we demonstrated, by three different methods, that RPE cells phagocytose T lymphocytes, in a time dependent manner. In additio ...
Tsao, Doris, Face Processing Mechanisms, Caltech, Apr
... slide: Anatomical tracer summary (diagram that includes all the above connections shown by their tracer studies) ...
... slide: Anatomical tracer summary (diagram that includes all the above connections shown by their tracer studies) ...
Activation of the p75 Neurotrophin Receptor through Conformational
... Receptor oligomerization has been recognized as a crucial step in the activation of many plasma membrane receptors. In receptors carrying intrinsic kinase activity, ligand-mediated dimerization stabilizes the active conformation of the kinase domain by receptor trans-phosphorylation (Schlessinger, 2 ...
... Receptor oligomerization has been recognized as a crucial step in the activation of many plasma membrane receptors. In receptors carrying intrinsic kinase activity, ligand-mediated dimerization stabilizes the active conformation of the kinase domain by receptor trans-phosphorylation (Schlessinger, 2 ...
Epithelial Integrin O/6~4: Complete Primary Structure of and Variant
... located in the 5'-untranslated region, and may encode regulatory sequences. Another specifies a segment of 70 amino acids in the cytoplasmic tail. Amplification by reverse transcription-polymerase chain reaction of mRNA indicated that multiple forms of ~4 may exist, possibly due to cell-type specifi ...
... located in the 5'-untranslated region, and may encode regulatory sequences. Another specifies a segment of 70 amino acids in the cytoplasmic tail. Amplification by reverse transcription-polymerase chain reaction of mRNA indicated that multiple forms of ~4 may exist, possibly due to cell-type specifi ...
Invest. Ophthalmol. Vis. Sci. 44: 1927-1932, 2003.
... them was 3% and 2.1% in the orbital and global layers, respectively. The satellite cells positive for each of these markers were randomly located within the total muscle cross-sectional area. Some areas were devoid of satellite cells positive for these markers, whereas other regions had many positiv ...
... them was 3% and 2.1% in the orbital and global layers, respectively. The satellite cells positive for each of these markers were randomly located within the total muscle cross-sectional area. Some areas were devoid of satellite cells positive for these markers, whereas other regions had many positiv ...
Cellular differentiation
In developmental biology, cellular differentiation isa cell changes from one cell type to another. Most commonly this is a less specialized type becoming a more specialized type, such as during cell growth. Differentiation occurs numerous times during the development of a multicellular organism as it changes from a simple zygote to a complex system of tissues and cell types. Differentiation continues in adulthood as adult stem cells divide and create fully differentiated daughter cells during tissue repair and during normal cell turnover. Some differentiation occurs in response to antigen exposure. Differentiation dramatically changes a cell's size, shape, membrane potential, metabolic activity, and responsiveness to signals. These changes are largely due to highly controlled modifications in gene expression and are the study of epigenetics. With a few exceptions, cellular differentiation almost never involves a change in the DNA sequence itself. Thus, different cells can have very different physical characteristics despite having the same genome.A cell that can differentiate into all cell types of the adult organism is known as pluripotent. Such cells are called embryonic stem cells in animals and meristematic cells in higher plants. A cell that can differentiate into all cell types, including the placental tissue, is known as totipotent. In mammals, only the zygote and subsequent blastomeres are totipotent, while in plants many differentiated cells can become totipotent with simple laboratory techniques. In cytopathology, the level of cellular differentiation is used as a measure of cancer progression. ""Grade"" is a marker of how differentiated a cell in a tumor is.