The C-terminal end of R-Ras contains a focal adhesion targeting
... R-Ras uses the endomembrane transport route To investigate the targeting of R-Ras, we constructed constitutive active (38V) and dominant-negative (43N) mutants of R-Ras and tagged them to EGFP. Western blot analysis of Hela cell extracts harbouring these constructs showed the presence of a 55 kDa pr ...
... R-Ras uses the endomembrane transport route To investigate the targeting of R-Ras, we constructed constitutive active (38V) and dominant-negative (43N) mutants of R-Ras and tagged them to EGFP. Western blot analysis of Hela cell extracts harbouring these constructs showed the presence of a 55 kDa pr ...
plant hormones lec9. ppt - An
... • Rooting morphogens (e.g., orthohydroxy phenol) some have been found, but not in all plants • Endogenous rooting inhibitors - have been shown to exist, but have not been identified • Rooting co-factors (auxin synergists) - several have been discovered (but not chemically identified) and no firm cau ...
... • Rooting morphogens (e.g., orthohydroxy phenol) some have been found, but not in all plants • Endogenous rooting inhibitors - have been shown to exist, but have not been identified • Rooting co-factors (auxin synergists) - several have been discovered (but not chemically identified) and no firm cau ...
Functional characterization of cohesin subunit SCC1 in
... structural maintenance of chromosome (SMC) complex (Losada and Hirano, 2005; Nasmyth and Haering, 2005). SMC complexes are composed of a SMC heterodimer, a kleisin and specific non-SMC subunits. In the case of cohesin, the a-kleisin is SCC1 which together with SMC1 and SMC3 forms a tripartite ring s ...
... structural maintenance of chromosome (SMC) complex (Losada and Hirano, 2005; Nasmyth and Haering, 2005). SMC complexes are composed of a SMC heterodimer, a kleisin and specific non-SMC subunits. In the case of cohesin, the a-kleisin is SCC1 which together with SMC1 and SMC3 forms a tripartite ring s ...
Probing the receptor recognition site of the FimH adhesin by f
... Expression is phase-variable with individual cells switching between a fimbriate and a non-fimbriate state. This is due to inversion of a 314 bp DNA segment located immediately upstream of the f;mA gene, encoding the major subunit protein (Fig. 1).A promoter residing in this phase switch drives the ...
... Expression is phase-variable with individual cells switching between a fimbriate and a non-fimbriate state. This is due to inversion of a 314 bp DNA segment located immediately upstream of the f;mA gene, encoding the major subunit protein (Fig. 1).A promoter residing in this phase switch drives the ...
Dominant-lethal alpha-tubulin mutants defective in microtubule depolymerization in yeast.
... Microtubules are cytoskeletal structures that function in eukaryotes to segregate chromosomes during cell division, position organelles, organize the cytoplasm, and provide form and motility to cilia and flagella. A central property of microtubules is that they are dynamic; at steady state, individu ...
... Microtubules are cytoskeletal structures that function in eukaryotes to segregate chromosomes during cell division, position organelles, organize the cytoplasm, and provide form and motility to cilia and flagella. A central property of microtubules is that they are dynamic; at steady state, individu ...
Novel, Secondary Sensory Cell Organ in Ascidians: In Search of the
... basal surface of the sensory cells. Micrographs were taken with a Hitachi H-600 electron microscope operated at 80 kV. To reveal acetylcholinesterase (AChE), tissue was treated following the method of Gautron (1982), prefixing the specimens in 1.5% glutaraldehyde buffered with 0.1 M sodium cacodylat ...
... basal surface of the sensory cells. Micrographs were taken with a Hitachi H-600 electron microscope operated at 80 kV. To reveal acetylcholinesterase (AChE), tissue was treated following the method of Gautron (1982), prefixing the specimens in 1.5% glutaraldehyde buffered with 0.1 M sodium cacodylat ...
The RNA-binding protein repertoire of embryonic
... in recent years been intensively characterized and used for genetic reprogramming3–7. The regulation and establishment of the stem cell at the post-transcriptional level is starting to be elucidated 8–11, paving the way for understanding combined regulatory networks at the DNA and RNA levels. RNAs i ...
... in recent years been intensively characterized and used for genetic reprogramming3–7. The regulation and establishment of the stem cell at the post-transcriptional level is starting to be elucidated 8–11, paving the way for understanding combined regulatory networks at the DNA and RNA levels. RNAs i ...
Listeria In Vivo Dynamic Imaging of the Effector Immune Response to Infection
... Funding: This work was supported by the National Institute of Health (Grant P01AI-071195). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing Interests: The authors have declared that no competing interests exist. * ...
... Funding: This work was supported by the National Institute of Health (Grant P01AI-071195). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing Interests: The authors have declared that no competing interests exist. * ...
Growing wrist mass
... On computed tomography (CT) the presence of haemosiderin deposits within the lesion may produce areas of high attenuation. As the lesion is hypervascular, contrast enhancement is often present. CT is also useful in detecting small erosions not visible or not clearly defined on radiographs.15–17 Gene ...
... On computed tomography (CT) the presence of haemosiderin deposits within the lesion may produce areas of high attenuation. As the lesion is hypervascular, contrast enhancement is often present. CT is also useful in detecting small erosions not visible or not clearly defined on radiographs.15–17 Gene ...
A Systems Survey of Progressive Host
... unbiased systems-level map of the host cellular processes involved, including RNA processing and translation, mTOR and MAPK signaling, organelle and membrane organization, and lipid metabolism. We then developed a complementary approach that harnesses cell-to-cell variability in infection progressio ...
... unbiased systems-level map of the host cellular processes involved, including RNA processing and translation, mTOR and MAPK signaling, organelle and membrane organization, and lipid metabolism. We then developed a complementary approach that harnesses cell-to-cell variability in infection progressio ...
The metabolism and functions of inositol pentakisphosphate and
... InsP, and InsP,, but not Ins/-’,, into the nucleus tractus solitarius (a discrete brain stem nucleus implicated in cardiovascular regulation) results in dose-dependent, reversible changes in heart rate and blood pressure. Furthermore, they showed that [3H]lnsl’, and [‘HIInsP, are synthesized in inta ...
... InsP, and InsP,, but not Ins/-’,, into the nucleus tractus solitarius (a discrete brain stem nucleus implicated in cardiovascular regulation) results in dose-dependent, reversible changes in heart rate and blood pressure. Furthermore, they showed that [3H]lnsl’, and [‘HIInsP, are synthesized in inta ...
The sea urchin immune system
... below), suggesting that pigment cells may have protective functions within the larval ectoderm. The blastocoelar cells develop from the secondary mesenchyme cells that migrate from the tip of the extending archenteron at late gastrulation (Tamboline and Burke, 1992). Approximately 20 of these cells ...
... below), suggesting that pigment cells may have protective functions within the larval ectoderm. The blastocoelar cells develop from the secondary mesenchyme cells that migrate from the tip of the extending archenteron at late gastrulation (Tamboline and Burke, 1992). Approximately 20 of these cells ...
Comparison of Autotransporter and Ice Nucleation Protein as Carrier
... Abstract Antibody surface display technology is critical for novel antibody screening and antibody affinity maturation. Currently most frequently used display methods are phage display and yeast display. Although Escherichia coli (E. coli) is easily cultured and genetically manipulated, thus is pote ...
... Abstract Antibody surface display technology is critical for novel antibody screening and antibody affinity maturation. Currently most frequently used display methods are phage display and yeast display. Although Escherichia coli (E. coli) is easily cultured and genetically manipulated, thus is pote ...
Remapping by hippocampal place cells
... Taking a slightly different view, in their elegant discussion of spatial context, Nadel and Willner (1980) argue that context is paradoxical: it both “is made up of” and “contains” the same stimuli. In other words, a given stimulus could be either a discrete cue or part of the context, depending on ...
... Taking a slightly different view, in their elegant discussion of spatial context, Nadel and Willner (1980) argue that context is paradoxical: it both “is made up of” and “contains” the same stimuli. In other words, a given stimulus could be either a discrete cue or part of the context, depending on ...
The more and smaller cells mutants of Arabidopsis
... development. In eudicot species, cell proliferation first occurs throughout the leaf primordium and is gradually restricted to the proximal part (Donnelly et al., 1999). Arrest of cell cycle and subsequent post-mitotic cell expansion occur from the distal to the proximal part of a young leaf. The re ...
... development. In eudicot species, cell proliferation first occurs throughout the leaf primordium and is gradually restricted to the proximal part (Donnelly et al., 1999). Arrest of cell cycle and subsequent post-mitotic cell expansion occur from the distal to the proximal part of a young leaf. The re ...
Catalases and PhoB/PhoR system independently contribute to
... cells electrophoretic profile- Fig. 1(c)) were excised, treated with trypsin and the resulting peptides ...
... cells electrophoretic profile- Fig. 1(c)) were excised, treated with trypsin and the resulting peptides ...
Microarray Analysis of Gene Expression in Saccharomyces
... reviews, see Wente and Rout 2010; Bauer et al. 2015). Importins function by associating with a nuclear localization signal (NLS) on the cargo protein to be imported, accompanying the NLS-containing cargo to the NPCs, and mediating translocation of the Kap/NLS-cargo complex through the nuclear pore. ...
... reviews, see Wente and Rout 2010; Bauer et al. 2015). Importins function by associating with a nuclear localization signal (NLS) on the cargo protein to be imported, accompanying the NLS-containing cargo to the NPCs, and mediating translocation of the Kap/NLS-cargo complex through the nuclear pore. ...
Chapter 13 - Fission Yeast TOR and Rapamycin
... Like nitrogen-starved cells, cells depleted of Tor2 divide a few times, accumulate at the G1 phase of the cell cycle and arrest their growth as small and round cells [11–15]. Since yeast cells normally grow in nature in the presence of abundant carbon but under nitrogen-limiting conditions, nitrogen ...
... Like nitrogen-starved cells, cells depleted of Tor2 divide a few times, accumulate at the G1 phase of the cell cycle and arrest their growth as small and round cells [11–15]. Since yeast cells normally grow in nature in the presence of abundant carbon but under nitrogen-limiting conditions, nitrogen ...
Mechanisms of cellular communication through intercellular protein
... immunologists [3–6]. Recently, the concept of synapse has been extended further to describe contacts between immune and nonimmune cells. Interestingly, a novel type of synapse termed as ‘stromal synapse’ identified between interstitial cells of Cajal (ICC)/ICC-like cells and immunoreactive cells, ap ...
... immunologists [3–6]. Recently, the concept of synapse has been extended further to describe contacts between immune and nonimmune cells. Interestingly, a novel type of synapse termed as ‘stromal synapse’ identified between interstitial cells of Cajal (ICC)/ICC-like cells and immunoreactive cells, ap ...
Document
... cell line (fro m Chinese Hamster Ovary (CHO) parent cell line) which expressed recombinant IGF-I that renders the cells to grow indifinitely in protein and/or serum-free media. Although this looks promising, the absence of serum generally will impose other issues such as induction of phenotypic chan ...
... cell line (fro m Chinese Hamster Ovary (CHO) parent cell line) which expressed recombinant IGF-I that renders the cells to grow indifinitely in protein and/or serum-free media. Although this looks promising, the absence of serum generally will impose other issues such as induction of phenotypic chan ...
Midbodies and phragmoplasts: analogous structures
... that has been compressed by the ingressing cleavage furrow (Figure 1a) and which will eventually reside within the intercellular canal (Glossary) that connects the two cells [9]. The midbody consists of a compact, dense matrix of proteins embedded in the region of microtubule overlap. In the early 1 ...
... that has been compressed by the ingressing cleavage furrow (Figure 1a) and which will eventually reside within the intercellular canal (Glossary) that connects the two cells [9]. The midbody consists of a compact, dense matrix of proteins embedded in the region of microtubule overlap. In the early 1 ...
Lineage-specific stem cells, signals and asymmetries
... (Iwafuchi-Doi and Zaret, 2014). For instance, ectopic expression of MyoD in fibroblasts induces muscle cell differentiation (Davis et al., 1987). The results of genome-wide MyoD binding studies indicate that MyoD binding requires specific E-boxes and is dependent on both interacting factors (coopera ...
... (Iwafuchi-Doi and Zaret, 2014). For instance, ectopic expression of MyoD in fibroblasts induces muscle cell differentiation (Davis et al., 1987). The results of genome-wide MyoD binding studies indicate that MyoD binding requires specific E-boxes and is dependent on both interacting factors (coopera ...
Cell Size Distributions of Soil Bacterial and Archaeal
... harbored distinct bacterial and archaeal communities in each of the three soils, and many of the taxa exhibited distinct size distribution patterns, with the smaller size fractions having higher relative abundances of taxa that are rare or poorly characterized (including Acidobacteria, Gemmatimonade ...
... harbored distinct bacterial and archaeal communities in each of the three soils, and many of the taxa exhibited distinct size distribution patterns, with the smaller size fractions having higher relative abundances of taxa that are rare or poorly characterized (including Acidobacteria, Gemmatimonade ...
Mediation of Clathrin-Dependent Trafficking during
... In plants, cytokinesis is mediated by the de novo assembly of the cell plate (CP) that forms a new cell wall (CW) and plasma membrane (PM). Beginning in late anaphase, secretory vesicles deliver lipids, proteins, and CW materials to the division plane and then fuse to initiate CP construction. Conti ...
... In plants, cytokinesis is mediated by the de novo assembly of the cell plate (CP) that forms a new cell wall (CW) and plasma membrane (PM). Beginning in late anaphase, secretory vesicles deliver lipids, proteins, and CW materials to the division plane and then fuse to initiate CP construction. Conti ...
Membrane-bound and extracellular P4actamase
... extracellular forms. Biochemical characterization of some of the properties of the enzyme showed that it belongs to the class A group of penicillinases. Comparison of the membrane-bound and extracellular forms of the & lactamases suggests that they seem to be differently processed forms of the same ...
... extracellular forms. Biochemical characterization of some of the properties of the enzyme showed that it belongs to the class A group of penicillinases. Comparison of the membrane-bound and extracellular forms of the & lactamases suggests that they seem to be differently processed forms of the same ...
Cellular differentiation
In developmental biology, cellular differentiation isa cell changes from one cell type to another. Most commonly this is a less specialized type becoming a more specialized type, such as during cell growth. Differentiation occurs numerous times during the development of a multicellular organism as it changes from a simple zygote to a complex system of tissues and cell types. Differentiation continues in adulthood as adult stem cells divide and create fully differentiated daughter cells during tissue repair and during normal cell turnover. Some differentiation occurs in response to antigen exposure. Differentiation dramatically changes a cell's size, shape, membrane potential, metabolic activity, and responsiveness to signals. These changes are largely due to highly controlled modifications in gene expression and are the study of epigenetics. With a few exceptions, cellular differentiation almost never involves a change in the DNA sequence itself. Thus, different cells can have very different physical characteristics despite having the same genome.A cell that can differentiate into all cell types of the adult organism is known as pluripotent. Such cells are called embryonic stem cells in animals and meristematic cells in higher plants. A cell that can differentiate into all cell types, including the placental tissue, is known as totipotent. In mammals, only the zygote and subsequent blastomeres are totipotent, while in plants many differentiated cells can become totipotent with simple laboratory techniques. In cytopathology, the level of cellular differentiation is used as a measure of cancer progression. ""Grade"" is a marker of how differentiated a cell in a tumor is.