... Novo) in 12 L pots filled with washed sand and nutrient solutions (Hoagland & Arnon, 1950) with 0.0 (deficient), 5.0 μmol L-1 (adequate) and 25.0 μmol L-1 (high) B. The pots were irrigated with nutrient solution twice a week. Two plants were grown per pot. When the first deficiency symptoms appeared ...
Heart valve tissue engineering: How far is the bedside from
... ageing, indicated the burden of these conditions would only likely increase in the future. The advent of organ transplantation has brought about the greatest progress in cardiac valve disease treatment so far, however, the number of patients that need heart valve replacements is rising by an average ...
... ageing, indicated the burden of these conditions would only likely increase in the future. The advent of organ transplantation has brought about the greatest progress in cardiac valve disease treatment so far, however, the number of patients that need heart valve replacements is rising by an average ...
Scalable Recombinant Adeno-Associated Virus Production
... spectrum of diseases (Aucoin et al., 2008). AAV is a dependovirus whose full life cycle is complemented by a helper virus, most often adenovirus or herpes simplex virus (Muzyczka and Berns, 2001). In addition, AAV wild-type infections lack pathological effects, conferring an added measure of safety ...
... spectrum of diseases (Aucoin et al., 2008). AAV is a dependovirus whose full life cycle is complemented by a helper virus, most often adenovirus or herpes simplex virus (Muzyczka and Berns, 2001). In addition, AAV wild-type infections lack pathological effects, conferring an added measure of safety ...
3 Adhesion and Adhesives of Fungi and Oomycetes
... complex and poorly characterized. As discussed in greater detail below (Sect. 3.4.2), fungi probably produce glycoprotein-(or proteoglycan-) based glues. Perhaps not surprisingly, the external layer of fungal cell walls is largely composed of heavily glycosylated proteins (de Nobel et al. 2001). Ind ...
... complex and poorly characterized. As discussed in greater detail below (Sect. 3.4.2), fungi probably produce glycoprotein-(or proteoglycan-) based glues. Perhaps not surprisingly, the external layer of fungal cell walls is largely composed of heavily glycosylated proteins (de Nobel et al. 2001). Ind ...
The Effect of Osmotic Shock on Release of Bacterial Proteins and on
... Leucine, isoleucine, and valine share a common transport system. Thus, the Km of entry (concentration resulting in half maximal initial rate of uptake) for both leucine and isoleucine is about 1 X 10-6 M, and the K~ for isoleucine when inhibiting the entry of leucine is also 1 X 10-e M. Further, at ...
... Leucine, isoleucine, and valine share a common transport system. Thus, the Km of entry (concentration resulting in half maximal initial rate of uptake) for both leucine and isoleucine is about 1 X 10-6 M, and the K~ for isoleucine when inhibiting the entry of leucine is also 1 X 10-e M. Further, at ...
Document
... Abstract— S erum; a clear portion of blood obtained after removing cells, platelets and clotting factors is a universal supplement commonly used in media for mammalian cell culture system. Serum contains amino acids, proteins, growth factors, hormones, vitamins, inorganic substances, nutrients and m ...
... Abstract— S erum; a clear portion of blood obtained after removing cells, platelets and clotting factors is a universal supplement commonly used in media for mammalian cell culture system. Serum contains amino acids, proteins, growth factors, hormones, vitamins, inorganic substances, nutrients and m ...
eosinophilia #1 - UCSF | Department of Medicine
... Eosinophilia AFL 7/30/02 Eosinophilia- Generally accepted as > 450 eos/microliter. Technically, eosinophilia is an accumulation of eos in the blood or tissue. Eos tightly regulated by body, usually only 1-3% of peripheral blood leukocytes. - small # in peripheral blood, and in tissues majority are i ...
... Eosinophilia AFL 7/30/02 Eosinophilia- Generally accepted as > 450 eos/microliter. Technically, eosinophilia is an accumulation of eos in the blood or tissue. Eos tightly regulated by body, usually only 1-3% of peripheral blood leukocytes. - small # in peripheral blood, and in tissues majority are i ...
PDF
... by centrifugation, such that some polar lobe material explore the hypothesis that localization and differential segregation of cytoplasmic factors are responsible for enters both blastomeres after the first cleavage, can the differences in fate that distinguish one cell from the produce mirror-symme ...
... by centrifugation, such that some polar lobe material explore the hypothesis that localization and differential segregation of cytoplasmic factors are responsible for enters both blastomeres after the first cleavage, can the differences in fate that distinguish one cell from the produce mirror-symme ...
Dictyostelium lysosomal proteins with different sugar modifications
... of marker enzymes for mitochondria (SIR, succinate-INT-reductase), endoplasmic reticulum (α-Glu-II, α-glucosidase-II) and protein. A Golgi marker (Ser:GlcNAc phosphotransferase) fractionated identically to α-Glu-II (not shown). All enzyme activities and antigen reactivity are expressed as percentage ...
... of marker enzymes for mitochondria (SIR, succinate-INT-reductase), endoplasmic reticulum (α-Glu-II, α-glucosidase-II) and protein. A Golgi marker (Ser:GlcNAc phosphotransferase) fractionated identically to α-Glu-II (not shown). All enzyme activities and antigen reactivity are expressed as percentage ...
PDF - Oxford Academic - Oxford University Press
... (dynein, kinesin and myosin). In addition, proteins normally found in the nucleus, like nucleolin and RNA helicase A, were also found associated with cytosolic hStaufen complexes. The co-localization of these components with hStaufen granules in the dendrites of differentiated neuroblasts, determine ...
... (dynein, kinesin and myosin). In addition, proteins normally found in the nucleus, like nucleolin and RNA helicase A, were also found associated with cytosolic hStaufen complexes. The co-localization of these components with hStaufen granules in the dendrites of differentiated neuroblasts, determine ...
GYP5 - Genetics
... shown to be a GAP for Ypt1p (DE ANTONI et al. 2002), a Rab GTPase involved in ER to Golgi trafficking (BACON et al. 1989, SEGEV, 1991; for review see LAZAR et al. 1997). Gyl1p is a protein with sequence similarity to Gyp5 and has been shown to colocalize with Gyp5p (CHESNEAU et al. 2004). We show th ...
... shown to be a GAP for Ypt1p (DE ANTONI et al. 2002), a Rab GTPase involved in ER to Golgi trafficking (BACON et al. 1989, SEGEV, 1991; for review see LAZAR et al. 1997). Gyl1p is a protein with sequence similarity to Gyp5 and has been shown to colocalize with Gyp5p (CHESNEAU et al. 2004). We show th ...
DOI: 10
... micropores immersed in the electrolyte is adjustable from 1 to 9 so that the pores can be functionalized individually or simultaneously in one step. A deposition buffer containing pyrrole (20 mM) and Py-ODN (5 µM) was introduced into the two electrolyte chambers on each side of the chip. A platinum ...
... micropores immersed in the electrolyte is adjustable from 1 to 9 so that the pores can be functionalized individually or simultaneously in one step. A deposition buffer containing pyrrole (20 mM) and Py-ODN (5 µM) was introduced into the two electrolyte chambers on each side of the chip. A platinum ...
Chapter 6 Histology of the Chorionic Villi, Fetal Membranes, and
... Throughout the first half of pregnancy, a limited number of true syncytial sprouts or trophoblastic sprouts (Figure 6.5) can be found. These are characterized by a vermiform extension from the villous surface with loosely arranged, large ovoid syncytial nuclei at the tip. Toward the villous stroma, p ...
... Throughout the first half of pregnancy, a limited number of true syncytial sprouts or trophoblastic sprouts (Figure 6.5) can be found. These are characterized by a vermiform extension from the villous surface with loosely arranged, large ovoid syncytial nuclei at the tip. Toward the villous stroma, p ...
GNOM-LIKE1/ERMO1 and SEC24a/ERMO2 Are
... and Walz, 2001). The outer nuclear envelope is continuous with the rough ER, and the rough ER is distinguished from smooth ER by the presence of ribosomes on the cytosolic side of the membrane. The ER forms a dynamic polygonal network composed of tubules, sheets, and three-way junctions. In addition ...
... and Walz, 2001). The outer nuclear envelope is continuous with the rough ER, and the rough ER is distinguished from smooth ER by the presence of ribosomes on the cytosolic side of the membrane. The ER forms a dynamic polygonal network composed of tubules, sheets, and three-way junctions. In addition ...
Inhibition of Hepatitis C Virus Genotype 1a Non
... may be useful for future clinical use as the treatment of certain infectious diseases, cancer and certain metabolic disorders [16]-[18]. Similarly, the phenomenon is highly effective at low dosage which makes it an excellent candidate for future clinical use [19]. Consequently, RNAi as a drug class ...
... may be useful for future clinical use as the treatment of certain infectious diseases, cancer and certain metabolic disorders [16]-[18]. Similarly, the phenomenon is highly effective at low dosage which makes it an excellent candidate for future clinical use [19]. Consequently, RNAi as a drug class ...
as a PDF
... among the limited number of T cell epitopes on gB that have been defined to date, there is sequence variation among H C M V strains which affects T cell responsiveness (Liu et al., 1991 b). Therefore, we wished to assess the response to gB by PBMC from additional donors expressing putative low respo ...
... among the limited number of T cell epitopes on gB that have been defined to date, there is sequence variation among H C M V strains which affects T cell responsiveness (Liu et al., 1991 b). Therefore, we wished to assess the response to gB by PBMC from additional donors expressing putative low respo ...
Should reflectance confocal microscopy be the gold standard for
... mostly contain basaloid cells and are mostly ellipsoidal in shape. They may also contain very bright, branched cells, which are consistent with melanocytes, and bright granules that correspond to melanosomes [7,8] . ‘Clefting’ is often observed using conventional histology and corresponds to darker ...
... mostly contain basaloid cells and are mostly ellipsoidal in shape. They may also contain very bright, branched cells, which are consistent with melanocytes, and bright granules that correspond to melanosomes [7,8] . ‘Clefting’ is often observed using conventional histology and corresponds to darker ...
Cytoskeletal rearrangements in gastric epithelial cells in response to
... only using AGS cells, but also in other epithelial cell types, including HEp-2 cells and T84 cells that have previously been used as models of H. pylori infection (Papini et al., 1998; Fahey et al., 2002). These findings indicate that the morphological changes induced by H. pylori are not restricted ...
... only using AGS cells, but also in other epithelial cell types, including HEp-2 cells and T84 cells that have previously been used as models of H. pylori infection (Papini et al., 1998; Fahey et al., 2002). These findings indicate that the morphological changes induced by H. pylori are not restricted ...
Symmetry, asymmetry, and the cell cycle in plants: known knowns
... intracellular components are organized in an asymmetric manner in the mother cell (Horvitz and Herskowitz, 1992; Petricka et al., 2009). The molecular mechanisms that control the asymmetry of cell divisions have been hypothesized to be tightly coupled to cell cycle timing and progression (Zhong, 200 ...
... intracellular components are organized in an asymmetric manner in the mother cell (Horvitz and Herskowitz, 1992; Petricka et al., 2009). The molecular mechanisms that control the asymmetry of cell divisions have been hypothesized to be tightly coupled to cell cycle timing and progression (Zhong, 200 ...
Extracellular matrix
In biology, the extracellular matrix (ECM) is a collection of extracellular molecules secreted by cells that provides structural and biochemical support to the surrounding cells. Because multicellularity evolved independently in different multicellular lineages, the composition of ECM varies between multicellular structures; however, cell adhesion, cell-to-cell communication and differentiation are common functions of the ECM.The animal extracellular matrix includes the interstitial matrix and the basement membrane. Interstitial matrix is present between various animal cells (i.e., in the intercellular spaces). Gels of polysaccharides and fibrous proteins fill the interstitial space and act as a compression buffer against the stress placed on the ECM. Basement membranes are sheet-like depositions of ECM on which various epithelial cells rest.The plant ECM includes cell wall components, like cellulose, in addition to more complex signaling molecules. Some single-celled organisms adopt multicelluar biofilms in which the cells are embedded in an ECM composed primarily of extracellular polymeric substances (EPS).