Networks Inferred from Biochemical Data Reveal Profound
... Reliability of Signaling Data and Derived Maps Two issues arise when comparing established cell lines with donorderived primary cells: (i) experiment-experiment and donor-donor variability and (ii) the purity of the primary cell preparations. To address the first issue, replicated assays of HepG2 ce ...
... Reliability of Signaling Data and Derived Maps Two issues arise when comparing established cell lines with donorderived primary cells: (i) experiment-experiment and donor-donor variability and (ii) the purity of the primary cell preparations. To address the first issue, replicated assays of HepG2 ce ...
Domain conservation in several volvocalean cell wall - UvA-DARE
... wall protein. The C-terminal rod domains of VSP-3 and WP6 are highly homologous, while the N-terminal domains are dissimilar; however, the N-terminal domain of VSP-3 is homologous to the globular domain of a cell wall protein from VoIvox carteri. Exon shuffling might be responsible for this example ...
... wall protein. The C-terminal rod domains of VSP-3 and WP6 are highly homologous, while the N-terminal domains are dissimilar; however, the N-terminal domain of VSP-3 is homologous to the globular domain of a cell wall protein from VoIvox carteri. Exon shuffling might be responsible for this example ...
Probing cytoplasmic viscosity in the confined geometry of
... provides excellent imaging capabilities. Furthermore, this model plant is particularly relevant for studies of drought tolerance [6–9], and for bio-technological applications [8, 10–12]. Nevertheless, in order to develop a mechanistic and quantitative understanding of plant cell growth, it is import ...
... provides excellent imaging capabilities. Furthermore, this model plant is particularly relevant for studies of drought tolerance [6–9], and for bio-technological applications [8, 10–12]. Nevertheless, in order to develop a mechanistic and quantitative understanding of plant cell growth, it is import ...
LIGHT MICROSCOPIC STUDY OF PORELLA PLATYPHYLLA (L
... -80°C. The reason for this is that the longer and slower freezing process could cause the formation of large ice crystals which destroy membrane systems and organelles too. Cells were unimpaired after the rapid and short frost effect at -80°C. When the cells were coloured with neutral red before fre ...
... -80°C. The reason for this is that the longer and slower freezing process could cause the formation of large ice crystals which destroy membrane systems and organelles too. Cells were unimpaired after the rapid and short frost effect at -80°C. When the cells were coloured with neutral red before fre ...
Placenta as a Source of Stem Cells and as a Key Organ for
... diseases.9-12 However, to date there is no concrete evidence to prove that fetal cells cause autoimmune disease,13 and increasing scientific evidence now suggests that these cells may actually help to combat disease. Support for this hypothesis comes from studies which show that fetal microchimerism ...
... diseases.9-12 However, to date there is no concrete evidence to prove that fetal cells cause autoimmune disease,13 and increasing scientific evidence now suggests that these cells may actually help to combat disease. Support for this hypothesis comes from studies which show that fetal microchimerism ...
Tumor-specific T cells in human Merkel cell carcinomas: a possible
... Paolini et al., 2011; Shuda et al., 2009; Shuda et al., 2008). 92% of MCC occur in immunocompetent individuals, and mortality is 30%, making MCC a more fatal cancer than malignant melanoma (Heath et al., 2008). The highly malignant nature of this virally mediated cancer suggests that MCC has potent ...
... Paolini et al., 2011; Shuda et al., 2009; Shuda et al., 2008). 92% of MCC occur in immunocompetent individuals, and mortality is 30%, making MCC a more fatal cancer than malignant melanoma (Heath et al., 2008). The highly malignant nature of this virally mediated cancer suggests that MCC has potent ...
FIP2 and Rip11 specify Rab11a-mediated cellular
... [13] and functions as an adapter protein for the direct interaction of Rab11a with the motor protein myosin-Vb [14]. A second interesting member of the class I FIPs is Rip11 which also co-localises with Rab11a in endosomal membranes [15]. To investigate the functional role of Rab11a and its effector ...
... [13] and functions as an adapter protein for the direct interaction of Rab11a with the motor protein myosin-Vb [14]. A second interesting member of the class I FIPs is Rip11 which also co-localises with Rab11a in endosomal membranes [15]. To investigate the functional role of Rab11a and its effector ...
glycolytic and associated enzymes of rainbow trout (oncorhynchus
... phosphorylase, red cell homogenates were not centrifuged or passed through a spun column; activity was measured directly in settled homogenates. Determination of enzyme maximal activities Enzyme activities in each red cell sample were determined in duplicate assays at 340 nm using a Gilford UV-VIS r ...
... phosphorylase, red cell homogenates were not centrifuged or passed through a spun column; activity was measured directly in settled homogenates. Determination of enzyme maximal activities Enzyme activities in each red cell sample were determined in duplicate assays at 340 nm using a Gilford UV-VIS r ...
Page 07-LS1-1. Conduct an investigation to provide evidence that
... http://www.ngsslifescience.com/biology_lesson_plans_human_body_systems.html https://www.pinterest.com/explore/human-body-lesson/ http://www.educationworld.com/a_lesson/lesson065.shtml http://www.lessonplansinc.com/science.php?/biology/lessonplans/C133 http://www.scholastic.com/teachers/activity/huma ...
... http://www.ngsslifescience.com/biology_lesson_plans_human_body_systems.html https://www.pinterest.com/explore/human-body-lesson/ http://www.educationworld.com/a_lesson/lesson065.shtml http://www.lessonplansinc.com/science.php?/biology/lessonplans/C133 http://www.scholastic.com/teachers/activity/huma ...
Embryonic development of the human hematopoietic system
... accessed from elective pregnancy interruptions. Yet, occasional histological investigations have indicated that the human yolk sac produces mostly erythroid cells (Bloom and Bartelmez, 1940). The occasional presence of macrophages and primitive megakaryocytes has been also described (Fukuda, 1973). ...
... accessed from elective pregnancy interruptions. Yet, occasional histological investigations have indicated that the human yolk sac produces mostly erythroid cells (Bloom and Bartelmez, 1940). The occasional presence of macrophages and primitive megakaryocytes has been also described (Fukuda, 1973). ...
Triton X-100 promotes a cholesterol
... temporal and spatial compartment for selected lipids and proteins and thus introduce specificity into processes such as signal transduction, cell migration and the dynamic sorting of membrane components [3–7]. Biologically, raft-mediated membrane sorting offers the cell two unique properties to over ...
... temporal and spatial compartment for selected lipids and proteins and thus introduce specificity into processes such as signal transduction, cell migration and the dynamic sorting of membrane components [3–7]. Biologically, raft-mediated membrane sorting offers the cell two unique properties to over ...
An in vitro System to Study Interactions between Bacteria and
... segments, the epithelial cells were harvested by centrifugation at 200 g for 10 rnin at 0 "C, gently resuspended, washed carefully in PBR (0 "C), and centrifuged again. The washed cells were gently suspended in cold Eagles MEM to a density of about 106-107 cells ml-l. When needed immediately, the ce ...
... segments, the epithelial cells were harvested by centrifugation at 200 g for 10 rnin at 0 "C, gently resuspended, washed carefully in PBR (0 "C), and centrifuged again. The washed cells were gently suspended in cold Eagles MEM to a density of about 106-107 cells ml-l. When needed immediately, the ce ...
The Effects of in Ovo Insulin-Like Growth Factor
... muscle fibers had come together; nevertheless, a complete bundle formation was not seen. Additionally, the proportion of intercellular connective tissue was excessive in comparison to the numbers of cells. The structure of the nuclei in the muscle cells appeared to be oval in most; however, some wer ...
... muscle fibers had come together; nevertheless, a complete bundle formation was not seen. Additionally, the proportion of intercellular connective tissue was excessive in comparison to the numbers of cells. The structure of the nuclei in the muscle cells appeared to be oval in most; however, some wer ...
Geometrical constraints in the scaling relationships between
... GS and other cell properties, where the independent variable is unclear [44] and relationships are not always very strong [5,11]. In such cases, it is necessary to estimate the scaling exponent by more appropriate techniques, such as standard major axis regression (SMA, sometimes also called reduced ...
... GS and other cell properties, where the independent variable is unclear [44] and relationships are not always very strong [5,11]. In such cases, it is necessary to estimate the scaling exponent by more appropriate techniques, such as standard major axis regression (SMA, sometimes also called reduced ...
Retinoic acid-mediated activation of HNF-3a
... 16 and 24 hours post-differentiation, reaches its zenith at approximately 1 day, and then declines to virtually undetectable levels. F9 cells can give rise to three distinct differentiated cell types; visceral endoderm, parietal endoderm, and primitive endoderm. We have clearly shown that HNF-3a sti ...
... 16 and 24 hours post-differentiation, reaches its zenith at approximately 1 day, and then declines to virtually undetectable levels. F9 cells can give rise to three distinct differentiated cell types; visceral endoderm, parietal endoderm, and primitive endoderm. We have clearly shown that HNF-3a sti ...
Structure and Nanostructure of the Outer Tangential Epidermal Cell
... Dynamic changes in the chemical composition as well as in the tissue structure during ripening, senescence, storage, post-harvest and processing of fruits cause variation in sensory, chemical and physical properties (Kunzek et al., 1999). In plants, the epidermal cell wall reduces the uncontrolled l ...
... Dynamic changes in the chemical composition as well as in the tissue structure during ripening, senescence, storage, post-harvest and processing of fruits cause variation in sensory, chemical and physical properties (Kunzek et al., 1999). In plants, the epidermal cell wall reduces the uncontrolled l ...
A myosin inhibitor impairs auxin
... day 8. During the second phase of the culture cycle, the frequency of unicellular files gradually recovers to the initial situation. At day 8, the majority of files consists of four or more cells. These files disintegrate subsequently, causing the recovery in the frequency of singular cells. In the ...
... day 8. During the second phase of the culture cycle, the frequency of unicellular files gradually recovers to the initial situation. At day 8, the majority of files consists of four or more cells. These files disintegrate subsequently, causing the recovery in the frequency of singular cells. In the ...
Fig. - Journal of Cell Science
... fingers (Stogios et al., 2005). No such domains have been described for KCTD proteins, yet the region comprising residues 114–237, which is located directly after its BTB domain, is sufficient for interaction with USP21 (Fig. 3A). The combined results of Y2H and pull-down analyses narrow down the US ...
... fingers (Stogios et al., 2005). No such domains have been described for KCTD proteins, yet the region comprising residues 114–237, which is located directly after its BTB domain, is sufficient for interaction with USP21 (Fig. 3A). The combined results of Y2H and pull-down analyses narrow down the US ...
University of Zurich - Institute of Molecular Life Sciences
... Macropinocytosis has been associated with human immunodeficiency virus 1 ...
... Macropinocytosis has been associated with human immunodeficiency virus 1 ...
chaudhari_project _2
... called as the metabolic pathways. Biotechnologists employ various methods to enhance the yields of metabolites that have practical significance. The main aim of the metabolic engineering is to optimize the metabolic network for maximizing the yields of necessary metabolites. It is important to know ...
... called as the metabolic pathways. Biotechnologists employ various methods to enhance the yields of metabolites that have practical significance. The main aim of the metabolic engineering is to optimize the metabolic network for maximizing the yields of necessary metabolites. It is important to know ...
Skb5, an SH3 adaptor protein, regulates Pmk1 MAPK signaling by
... Pck2 in wild-type (WT) cells results in severe growth defects, whereas the inhibition or deletion of the components of the Pmk1 MAPK pathway can reverse the growth defects. Consistent with this, the overproduction of protein from the pmp1+ gene, which we previously identified as a dual-specificity p ...
... Pck2 in wild-type (WT) cells results in severe growth defects, whereas the inhibition or deletion of the components of the Pmk1 MAPK pathway can reverse the growth defects. Consistent with this, the overproduction of protein from the pmp1+ gene, which we previously identified as a dual-specificity p ...
Intracellular catalysis of disulfide bond formation by the human
... Many secreted proteins contain disulfide bonds, which are required for their proper folding, function and stability. In eukaryotic cells disulfide bond formation usually occurs while the protein folds in the lumen of the ER (endoplasmic reticulum). While disulfide bonds can be formed spontaneously i ...
... Many secreted proteins contain disulfide bonds, which are required for their proper folding, function and stability. In eukaryotic cells disulfide bond formation usually occurs while the protein folds in the lumen of the ER (endoplasmic reticulum). While disulfide bonds can be formed spontaneously i ...
Electron Microscopic Observations on the Excretion of Cell
... virtue of the plasticity of the cellular membrane that substances which cannot diffuse through it may be incorporated by, for example, pinocytosis (Lewis, 1931; Palade, 1953) or may be excreted from the cells. Although there may exist several mechanisms for the excretion or secretion of cellular pro ...
... virtue of the plasticity of the cellular membrane that substances which cannot diffuse through it may be incorporated by, for example, pinocytosis (Lewis, 1931; Palade, 1953) or may be excreted from the cells. Although there may exist several mechanisms for the excretion or secretion of cellular pro ...
Differences between Edexcel `A` and `B`
... Be able to distinguish between Gram positive and Gram negative bacterial cell walls and understand why each type reacts differently to some antibiotics. Know how magnification and resolution can be achieved using light and electron microscopy Understand the importance of staining specimens in micros ...
... Be able to distinguish between Gram positive and Gram negative bacterial cell walls and understand why each type reacts differently to some antibiotics. Know how magnification and resolution can be achieved using light and electron microscopy Understand the importance of staining specimens in micros ...
Extracellular matrix
In biology, the extracellular matrix (ECM) is a collection of extracellular molecules secreted by cells that provides structural and biochemical support to the surrounding cells. Because multicellularity evolved independently in different multicellular lineages, the composition of ECM varies between multicellular structures; however, cell adhesion, cell-to-cell communication and differentiation are common functions of the ECM.The animal extracellular matrix includes the interstitial matrix and the basement membrane. Interstitial matrix is present between various animal cells (i.e., in the intercellular spaces). Gels of polysaccharides and fibrous proteins fill the interstitial space and act as a compression buffer against the stress placed on the ECM. Basement membranes are sheet-like depositions of ECM on which various epithelial cells rest.The plant ECM includes cell wall components, like cellulose, in addition to more complex signaling molecules. Some single-celled organisms adopt multicelluar biofilms in which the cells are embedded in an ECM composed primarily of extracellular polymeric substances (EPS).