Integrin cytoplasmic domain-binding proteins
... proximal ten residues of β1. Under some conditions, skelemin colocalizes with αIIbβ3 expressed in CHO cells, and microinjection of the integrin-binding domain of skelemin causes myoblasts to round up (Reddy et al., 1998), conceivably by disrupting integrin-cytoskeleton interactions. Skelemin is enco ...
... proximal ten residues of β1. Under some conditions, skelemin colocalizes with αIIbβ3 expressed in CHO cells, and microinjection of the integrin-binding domain of skelemin causes myoblasts to round up (Reddy et al., 1998), conceivably by disrupting integrin-cytoskeleton interactions. Skelemin is enco ...
Cell-surface location of Listeria-specific protein p60
... emphasize the need for more rapid and reliable detection procedures. The main interest has focused on genetic and immunoassay methods (Butman et al., 1988; McLauchlin et al., 1989; Kohler et al., 1990; Siragusa & Johnson, 1990; Wang et al., 1991). We have prepared a specific antiserum against the 60 ...
... emphasize the need for more rapid and reliable detection procedures. The main interest has focused on genetic and immunoassay methods (Butman et al., 1988; McLauchlin et al., 1989; Kohler et al., 1990; Siragusa & Johnson, 1990; Wang et al., 1991). We have prepared a specific antiserum against the 60 ...
of Limb Morphogenesis in a Model System
... properties, but has not yet begun to exhibit the cartilage tissue found in the stage 25 proximal mesoblast. Since a population of cells in this stage of the limb are not homogeneous with respect to cytodifferentiation as are chondrocytes from stage 30, or morphogenetic expression, as are stage 19 ce ...
... properties, but has not yet begun to exhibit the cartilage tissue found in the stage 25 proximal mesoblast. Since a population of cells in this stage of the limb are not homogeneous with respect to cytodifferentiation as are chondrocytes from stage 30, or morphogenetic expression, as are stage 19 ce ...
Bacterial Filament Systems: Toward Understanding Their Emergent
... the membrane. Together, these activities cause all three components to oscillate between cell poles, creating a time-averaged minimum of MinC at mid-cell (53). Reconstitutions of MinCDE and FtsZ in three-dimensional cell-shaped compartments demonstrated that MinCDE together form a time-averaged bipo ...
... the membrane. Together, these activities cause all three components to oscillate between cell poles, creating a time-averaged minimum of MinC at mid-cell (53). Reconstitutions of MinCDE and FtsZ in three-dimensional cell-shaped compartments demonstrated that MinCDE together form a time-averaged bipo ...
pdf - Penn State University
... other hemicelluloses are found tightly bound to cellulose, and they may also help anchor the microfibril to the matrix (reviewed in Cosgrove, 1997). Binding studies (Hayashi, 1989) suggest that xyloglucan does not simply bind to the microfibril surface, but much of it may also be entrapped within th ...
... other hemicelluloses are found tightly bound to cellulose, and they may also help anchor the microfibril to the matrix (reviewed in Cosgrove, 1997). Binding studies (Hayashi, 1989) suggest that xyloglucan does not simply bind to the microfibril surface, but much of it may also be entrapped within th ...
A DNA Damage Checkpoint Pathway Coordinates the
... Regardless of the manner in which the distinct nuclei are sorted, an important feature during nuclear division in dikaryotic cells is that even when nuclei are located in different cell compartments—one in the main hypha and the other in the clamp cell—the two nuclei have to divide synchronously (Ta ...
... Regardless of the manner in which the distinct nuclei are sorted, an important feature during nuclear division in dikaryotic cells is that even when nuclei are located in different cell compartments—one in the main hypha and the other in the clamp cell—the two nuclei have to divide synchronously (Ta ...
Microtubules do not promote mitotic slippage when the spindle
... At this point, we can conclude that in the absence of MTs, the duration of mitosis in RPE-1 cells varies from ⵑ20 to 30 h depending on the MT poison. We next asked if the mere presence of MTs accelerates mitotic slippage. In our first study on this question, we treated RPE-1 cells entering mitosis w ...
... At this point, we can conclude that in the absence of MTs, the duration of mitosis in RPE-1 cells varies from ⵑ20 to 30 h depending on the MT poison. We next asked if the mere presence of MTs accelerates mitotic slippage. In our first study on this question, we treated RPE-1 cells entering mitosis w ...
Regulation of translesion synthesis DNA polymerase by η
... We next attempted to identify the lysine residue(s) in polη that can be ubiquitinated. HEK293T cells were transfected with HA-tagged ubiquitin and Flag-tagged polη. After immunoprecipitation of polη with anti-Flag antibodies, the presumptive monoubiquitinated species of polη was visualized by Coomas ...
... We next attempted to identify the lysine residue(s) in polη that can be ubiquitinated. HEK293T cells were transfected with HA-tagged ubiquitin and Flag-tagged polη. After immunoprecipitation of polη with anti-Flag antibodies, the presumptive monoubiquitinated species of polη was visualized by Coomas ...
Induced pluripotent stem cells: methods - Tubitak Journals
... and Yamanaka’s (2006) ground-breaking research findings involved the initial screening of 24 TFs for their reprogramming potential using a combination of reporter gene technology and a neomycin resistance marker. The resistance marker enabled the identification of cells with the embryonic stem cell- ...
... and Yamanaka’s (2006) ground-breaking research findings involved the initial screening of 24 TFs for their reprogramming potential using a combination of reporter gene technology and a neomycin resistance marker. The resistance marker enabled the identification of cells with the embryonic stem cell- ...
Exine dehiscing induces rape microspore polarity
... Although not essential for plant growth, in terrestrial seagrass Posidonia oceanica (L.) Delile plays a relevant role plants, Cd is readily absorbed by roots and translocated into by ensuring primary production, water oxygenation and aerial organs while, in acquatic plants, it is directly taken up I ...
... Although not essential for plant growth, in terrestrial seagrass Posidonia oceanica (L.) Delile plays a relevant role plants, Cd is readily absorbed by roots and translocated into by ensuring primary production, water oxygenation and aerial organs while, in acquatic plants, it is directly taken up I ...
Blimp1 regulates development of the posterior forelimb, caudal
... inactivation strategy. A Blimp1-Cre transgenic strain was also exploited to generate a fate map of Blimp1-expressing cells. Blimp1 plays essential roles in multipotent progenitor cell populations in the posterior forelimb, caudal pharyngeal arches, secondary heart field and sensory vibrissae and mai ...
... inactivation strategy. A Blimp1-Cre transgenic strain was also exploited to generate a fate map of Blimp1-expressing cells. Blimp1 plays essential roles in multipotent progenitor cell populations in the posterior forelimb, caudal pharyngeal arches, secondary heart field and sensory vibrissae and mai ...
Primitive streak mesoderm-like cell lines expressing
... factor (LIF) blocks differentiation of the mesodermal lineages formed following treatment of P19 EC cells with DMSO (Pruitt and Natoli, 1992). Additionally, when LIF is present during either DMSO or RA treatment of P19 cell cultures, these cultures fail to express Pax-3-inducing activity consistent ...
... factor (LIF) blocks differentiation of the mesodermal lineages formed following treatment of P19 EC cells with DMSO (Pruitt and Natoli, 1992). Additionally, when LIF is present during either DMSO or RA treatment of P19 cell cultures, these cultures fail to express Pax-3-inducing activity consistent ...
BioWire_Progress_Report_Week_One_Rev_1
... [BioBrick and AHL should arrive by Tuesday] Manually add AHL to the system in varying concentrations Control: Add water instead of AHL BioBrick comes with YFP as a reporter Expected Result: YFP is expressed, but not in control plates. Note: replicate experiment using GFP/mCherry as decided ...
... [BioBrick and AHL should arrive by Tuesday] Manually add AHL to the system in varying concentrations Control: Add water instead of AHL BioBrick comes with YFP as a reporter Expected Result: YFP is expressed, but not in control plates. Note: replicate experiment using GFP/mCherry as decided ...
Asymmetries in Cell Division, Cell Size, and Furrowing in the
... J.-P. Tassan, J.Z. Kubiak (eds.), Asymmetric Cell Division in Development, Differentiation and Cancer, Results and Problems in Cell Differentiation 61, DOI 10.1007/978-3-319-53150-2_11 ...
... J.-P. Tassan, J.Z. Kubiak (eds.), Asymmetric Cell Division in Development, Differentiation and Cancer, Results and Problems in Cell Differentiation 61, DOI 10.1007/978-3-319-53150-2_11 ...
FoxF is essential for FGF-induced migration of heart
... knock-down showed that Mesp activity is required for both TVC migration in the embryo and cardiomyocyte differentiation during metamorphosis (Satou et al., 2004). In addition, ascidian orthologs of the conserved heart specification genes NK-4 (tinman/Nkx2.5), GATA-a (pannier/GATA4/5/6), Hand and Han ...
... knock-down showed that Mesp activity is required for both TVC migration in the embryo and cardiomyocyte differentiation during metamorphosis (Satou et al., 2004). In addition, ascidian orthologs of the conserved heart specification genes NK-4 (tinman/Nkx2.5), GATA-a (pannier/GATA4/5/6), Hand and Han ...
Diefenbach, A., and D.H. Raulet. 2002. The innate immune response to tumors and its role in the induction of T cell immunity. Immunological Reviews 188:9-21.
... rapidly disappeared after irradiation. It was shown that the rapid disappearance of these cells in normal mice is mediated by NK cells (56). In analogous cohorts of humans, such as survivors of atomic bombs or the Chernobyl nuclear plant accident, increased frequencies of mutations were documented i ...
... rapidly disappeared after irradiation. It was shown that the rapid disappearance of these cells in normal mice is mediated by NK cells (56). In analogous cohorts of humans, such as survivors of atomic bombs or the Chernobyl nuclear plant accident, increased frequencies of mutations were documented i ...
SUMO Pathway Modulation of Regulatory Protein Binding
... reduction in rDNA binding in ulp2D cells, which is also rescued by elimination of Slx5. Additionally, overexpression of Sir2, another RFBassociated factor, suppresses the growth defect of ulp2D cells. Our data suggest that regulation of rDNA regulatory proteins by Ulp2 and the Slx5/Slx8 STUbL may be ...
... reduction in rDNA binding in ulp2D cells, which is also rescued by elimination of Slx5. Additionally, overexpression of Sir2, another RFBassociated factor, suppresses the growth defect of ulp2D cells. Our data suggest that regulation of rDNA regulatory proteins by Ulp2 and the Slx5/Slx8 STUbL may be ...
Localization of the Microtubule End Binding Protein
... Otegui and Staehelin, 2000). This, the phragmoplast, then grows out centrifugally, opening up as a ring that deposits the cell plate in its center. Microtubule hook decoration studies have shown that phragmoplast microtubules share the same polarity as those of the spindle, with the plus ends overla ...
... Otegui and Staehelin, 2000). This, the phragmoplast, then grows out centrifugally, opening up as a ring that deposits the cell plate in its center. Microtubule hook decoration studies have shown that phragmoplast microtubules share the same polarity as those of the spindle, with the plus ends overla ...
Fatty acids as gatekeepers of immune cell regulation - Direct-MS
... prolonged inflammatory responses [35], suggesting that PPARs could be anti-inflammatory. PPARa is the predominant isoform expressed in murine T and B lymphocytes, whereas PPARg dominates in myeloid cells [36]. Following activation of T cells, PPARa expression is decreased, whereas PPARg expression i ...
... prolonged inflammatory responses [35], suggesting that PPARs could be anti-inflammatory. PPARa is the predominant isoform expressed in murine T and B lymphocytes, whereas PPARg dominates in myeloid cells [36]. Following activation of T cells, PPARa expression is decreased, whereas PPARg expression i ...
University of Groningen How to get (a)round Pinho, Mariana
... currently unknown. The complete septum is composed of a low-density central layer separating two high-density layers, corresponding to adjacent cross walls, which will each become one-half of the new cell wall in daughter cells. S. aureus encodes multiple autolysins, but thus far only Atl is known t ...
... currently unknown. The complete septum is composed of a low-density central layer separating two high-density layers, corresponding to adjacent cross walls, which will each become one-half of the new cell wall in daughter cells. S. aureus encodes multiple autolysins, but thus far only Atl is known t ...
Accessory Organs of the Small Intestine
... sinusoid system for the secretion of substances into the blood. This association with the sinusoids also allows for the recovery of nutrients arriving from the digestive tract through the hepatic portal system. The hepatocytes store these nutrients and will release them into the blood as needed. Ex; ...
... sinusoid system for the secretion of substances into the blood. This association with the sinusoids also allows for the recovery of nutrients arriving from the digestive tract through the hepatic portal system. The hepatocytes store these nutrients and will release them into the blood as needed. Ex; ...
Lymphoma Vaccines for Active Immunotherapy of T Cell Are More
... The ability to target multiple tumor Ags may increase the magnitude and diversity of anti-tumor responses, thus preventing tumor from escaping responses limited in repertoire (30). Loading DC with proteins from tumor cell lysates may result in the presentation of a broader array of tumor-specific Ag ...
... The ability to target multiple tumor Ags may increase the magnitude and diversity of anti-tumor responses, thus preventing tumor from escaping responses limited in repertoire (30). Loading DC with proteins from tumor cell lysates may result in the presentation of a broader array of tumor-specific Ag ...
human embryonic stem cells and their clinical relevance
... Human embryonic stem cells should offer insights into development events that cannot be studied directly in the intact human embryo but that have important consequences in clinical areas, including birth defects, infertility and pregnancy loss [31]. Recently, human embryonic stem cells have also bee ...
... Human embryonic stem cells should offer insights into development events that cannot be studied directly in the intact human embryo but that have important consequences in clinical areas, including birth defects, infertility and pregnancy loss [31]. Recently, human embryonic stem cells have also bee ...
Extracellular matrix
In biology, the extracellular matrix (ECM) is a collection of extracellular molecules secreted by cells that provides structural and biochemical support to the surrounding cells. Because multicellularity evolved independently in different multicellular lineages, the composition of ECM varies between multicellular structures; however, cell adhesion, cell-to-cell communication and differentiation are common functions of the ECM.The animal extracellular matrix includes the interstitial matrix and the basement membrane. Interstitial matrix is present between various animal cells (i.e., in the intercellular spaces). Gels of polysaccharides and fibrous proteins fill the interstitial space and act as a compression buffer against the stress placed on the ECM. Basement membranes are sheet-like depositions of ECM on which various epithelial cells rest.The plant ECM includes cell wall components, like cellulose, in addition to more complex signaling molecules. Some single-celled organisms adopt multicelluar biofilms in which the cells are embedded in an ECM composed primarily of extracellular polymeric substances (EPS).