R on Dendritic Cells - The Journal of Immunology
... plates were inoculated into Todd-Hewitt broth with 0.5% yeast extract and grown to mid-log phase. The concentration of bacteria was estimated by absorbance at 600 nm and verified by plate counts. R36a were washed into PBS and heated at 60°C for 60 min to kill the bacteria and destroy pneumolysin act ...
... plates were inoculated into Todd-Hewitt broth with 0.5% yeast extract and grown to mid-log phase. The concentration of bacteria was estimated by absorbance at 600 nm and verified by plate counts. R36a were washed into PBS and heated at 60°C for 60 min to kill the bacteria and destroy pneumolysin act ...
Micronuclei Bearing Acentric Extrachromosomal Chromatin Are
... cell cycle (13). We also observed this when we tracked micronuclei in H2B-green fluorescent protein (GFP) fusion protein-expressing HeLa cells.1 Thus, micronuclei elimination may occur infrequently and/or require a specific stage of the cell cycle. These observations indicate that micronuclei can pe ...
... cell cycle (13). We also observed this when we tracked micronuclei in H2B-green fluorescent protein (GFP) fusion protein-expressing HeLa cells.1 Thus, micronuclei elimination may occur infrequently and/or require a specific stage of the cell cycle. These observations indicate that micronuclei can pe ...
Full Text - The International Journal of Developmental Biology
... to the cellular and subcellular levels and, from the 1950’s on, to the molecular level, transformed experimental embryology to developmental biology”. The term “decline” of experimental embryology was falsely interpreted by some authors as a decline of the Freiburg school. That this opinion is wrong ...
... to the cellular and subcellular levels and, from the 1950’s on, to the molecular level, transformed experimental embryology to developmental biology”. The term “decline” of experimental embryology was falsely interpreted by some authors as a decline of the Freiburg school. That this opinion is wrong ...
Defect in neutrophil killing and increased
... (pKOS/PGRP-S/45, pKOS/PGRP-S/40, and pKOS/PGRP-S/29), which contained all 3 PGRP-S exons (Figure 1A). The contigs containing exon 1 and exons 2 and 3 were sequenced (GenBank accession nos. AY144360 and AY144361, respectively). The targeting vector was constructed from pKOS/PGRP-S/40 using a yeast-me ...
... (pKOS/PGRP-S/45, pKOS/PGRP-S/40, and pKOS/PGRP-S/29), which contained all 3 PGRP-S exons (Figure 1A). The contigs containing exon 1 and exons 2 and 3 were sequenced (GenBank accession nos. AY144360 and AY144361, respectively). The targeting vector was constructed from pKOS/PGRP-S/40 using a yeast-me ...
Studies on the Contribution of c-fos/AP-1 to Arthritic Joint
... and tumor necrosis factor a, which are important in RA and as a major constituent of invasive granulation tissue called pannus, play important roles especially in the perpetuation of arthritis and subsequent joint destruction (1, 4, 5). We have studied the role of c-fos protooncogene in arthritis be ...
... and tumor necrosis factor a, which are important in RA and as a major constituent of invasive granulation tissue called pannus, play important roles especially in the perpetuation of arthritis and subsequent joint destruction (1, 4, 5). We have studied the role of c-fos protooncogene in arthritis be ...
Polyunsaturated fatty acids modulate NOX 4 anion superoxide
... integral membrane proteins making up flavocytochrome b558 and the catalytic unit of the protein complex responsible for the oxidative burst in polymorphonuclear cells, have been identified in mammals [1–4]. The identification of new members of this family of enzymes has been an important step in und ...
... integral membrane proteins making up flavocytochrome b558 and the catalytic unit of the protein complex responsible for the oxidative burst in polymorphonuclear cells, have been identified in mammals [1–4]. The identification of new members of this family of enzymes has been an important step in und ...
Suppressor of Hairless-independent events in
... effector of Notch since the expression of bHLH genes encoded by E(spl) depends on both Notch signaling and the presence of Su(H)-binding sites in the promoters (Jennings et al., 1994; Lecourtois and Schweisguth, 1995; Bailey and Posakony, 1995). Truncation of the extracellular domain of Notch result ...
... effector of Notch since the expression of bHLH genes encoded by E(spl) depends on both Notch signaling and the presence of Su(H)-binding sites in the promoters (Jennings et al., 1994; Lecourtois and Schweisguth, 1995; Bailey and Posakony, 1995). Truncation of the extracellular domain of Notch result ...
Cell Research
... Xenopus oocytes, whereas PIP1 proteins are often inactive or have low activity [7]. Some PIP1 members may not be related to water movement across the membrane but they might act as transporters for small solutes or gases. To investigate genes involved in the control of Medicago truncatula germinatio ...
... Xenopus oocytes, whereas PIP1 proteins are often inactive or have low activity [7]. Some PIP1 members may not be related to water movement across the membrane but they might act as transporters for small solutes or gases. To investigate genes involved in the control of Medicago truncatula germinatio ...
PDF - Journal of Neuroscience
... The RE1 Silencing Transcription Factor (REST) acts as a governor of the mature neuronal phenotype by repressing a large consortium of neuronal genes in non-neuronal cells. In the developing nervous system, REST is present in progenitors and downregulated at terminal differentiation to promote acquis ...
... The RE1 Silencing Transcription Factor (REST) acts as a governor of the mature neuronal phenotype by repressing a large consortium of neuronal genes in non-neuronal cells. In the developing nervous system, REST is present in progenitors and downregulated at terminal differentiation to promote acquis ...
Dev Biol 364(2), 138-48. PDF
... Background-dependent embryonic lethality in Pk2 −/− mouse embryos To study the in vivo function of Pk2, we generated knock-out mice lacking PET/LIM, one of the functional domains of Pk2 (Tao et al., 2011). In the Pk2 −/− embryos, no Pk2 protein was observed at the 8-cell stage (n = 8/8) (Fig. S3B), ...
... Background-dependent embryonic lethality in Pk2 −/− mouse embryos To study the in vivo function of Pk2, we generated knock-out mice lacking PET/LIM, one of the functional domains of Pk2 (Tao et al., 2011). In the Pk2 −/− embryos, no Pk2 protein was observed at the 8-cell stage (n = 8/8) (Fig. S3B), ...
Mutations in the Arabidopsis RPK1 gene uncouple cotyledon
... mutants manifest in a spatially and timely stochastic fashion probably due to redundancy of RPK1-like functions. ...
... mutants manifest in a spatially and timely stochastic fashion probably due to redundancy of RPK1-like functions. ...
Cell Alloimmunization Hemin Controls T Cell Polarization in Sickle
... For Treg subset analysis at day 7 of CD4+ T cell/monocyte cocultures, cells were harvested and intracellular expression of FOXP3 and Helios in CD4+ cells was analyzed using anti-FOXP3 (clone PCH101; eBioscience, San Jose, CA) and anti-Helios (clone 22F6; BioLegend, San Diego, CA), respectively, as p ...
... For Treg subset analysis at day 7 of CD4+ T cell/monocyte cocultures, cells were harvested and intracellular expression of FOXP3 and Helios in CD4+ cells was analyzed using anti-FOXP3 (clone PCH101; eBioscience, San Jose, CA) and anti-Helios (clone 22F6; BioLegend, San Diego, CA), respectively, as p ...
Cells To Tolerate Higher Expression of Bim T+ Bcl
... of Bcl-2 in the survival of memory T cells is somewhat controversial. For example, Y449 in the cytoplasmic domain of IL-7Ra was shown to be critical for survival of memory T cells but not for IL-7–driven Bcl-2 upregulation (17), suggesting that antiapoptotic signals other than Bcl-2 control memory T ...
... of Bcl-2 in the survival of memory T cells is somewhat controversial. For example, Y449 in the cytoplasmic domain of IL-7Ra was shown to be critical for survival of memory T cells but not for IL-7–driven Bcl-2 upregulation (17), suggesting that antiapoptotic signals other than Bcl-2 control memory T ...
Looking at Kaposi`s sarcoma-associated herpesvirus–host
... Since the discovery of miRNA, we have known that it modulates gene expression post-transcriptionally through targeting the 3 UTRs of target genes or through inducing degradation of target gene transcripts (Lee et al., 1993). The expression of miRNAs themselves is also strictly regulated through dif ...
... Since the discovery of miRNA, we have known that it modulates gene expression post-transcriptionally through targeting the 3 UTRs of target genes or through inducing degradation of target gene transcripts (Lee et al., 1993). The expression of miRNAs themselves is also strictly regulated through dif ...
Regeneration and Phenotype That Promote Muscle Growth IL
... We examined the function of IL-10 in regulating changes in macrophage phenotype during muscle growth and regeneration following injury. Our findings showed that the Th1 cytokine response in inflamed muscle is characterized by high levels of expression of CD68, CCL-2, TNF-a, and IL-6 at 1 d postinjur ...
... We examined the function of IL-10 in regulating changes in macrophage phenotype during muscle growth and regeneration following injury. Our findings showed that the Th1 cytokine response in inflamed muscle is characterized by high levels of expression of CD68, CCL-2, TNF-a, and IL-6 at 1 d postinjur ...
Auxins in defense strategies
... the endogenous IAA level significantly increases and expression of some auxin-regulated genes is induced. Auxin transiently causes transcription of three primary gene families: SMALL AUXIN UP RNA (SAUR), AUX/IAA and GRETCHEN HAGEN 3 (GH3) (Guilfoyle 1999; Hagen & Guilfoyle 2002). SAUR genes were orig ...
... the endogenous IAA level significantly increases and expression of some auxin-regulated genes is induced. Auxin transiently causes transcription of three primary gene families: SMALL AUXIN UP RNA (SAUR), AUX/IAA and GRETCHEN HAGEN 3 (GH3) (Guilfoyle 1999; Hagen & Guilfoyle 2002). SAUR genes were orig ...
Enhancement of Hypothalamic STAT3 Acetylation by
... 1E). Detection of a series of relevant gene products supports the role of Nur77 on lipid metabolism. In WAT, the gene expressions for lipid synthesis were decreased in leptin-treated WT mice (such as SREBP1c, FASN, ACC, and SCD1); in contrast, there was no obvious change in KO mice. Conversely, the ...
... 1E). Detection of a series of relevant gene products supports the role of Nur77 on lipid metabolism. In WAT, the gene expressions for lipid synthesis were decreased in leptin-treated WT mice (such as SREBP1c, FASN, ACC, and SCD1); in contrast, there was no obvious change in KO mice. Conversely, the ...
Cell death by autophagy: facts and apparent artefacts
... and metazoans, and involves the action of the canonical Atg genes as described above. The process of autophagic/ vacuolar cell death in plants resembles metazoan autophagy, and examples of autophagic/vacuolar cell deaths during plant development and under stress conditions are beginning to be reveal ...
... and metazoans, and involves the action of the canonical Atg genes as described above. The process of autophagic/ vacuolar cell death in plants resembles metazoan autophagy, and examples of autophagic/vacuolar cell deaths during plant development and under stress conditions are beginning to be reveal ...
