Growth Cones Are Not Required for Initial Establishment of Polarity
... believed to be free of cellular effects other than its inhibition of actin polymer assembly. This variety has also been shown to be effective for long-term treatment of cultured neurons without compromising cell survival (Morris and Hollenbeck, 1995). Doses in the range of 0.17– 0.23 g/ml CE were u ...
... believed to be free of cellular effects other than its inhibition of actin polymer assembly. This variety has also been shown to be effective for long-term treatment of cultured neurons without compromising cell survival (Morris and Hollenbeck, 1995). Doses in the range of 0.17– 0.23 g/ml CE were u ...
Meaningful relationships: the regulation of the Ras/Raf/MEK/ERK
... The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, U.K. ...
... The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, U.K. ...
Spore Formation Bacillus subtilis during Compartmentalization of
... cell, which releases the mature spore into the environment. ...
... cell, which releases the mature spore into the environment. ...
Full and Partial Agonists of Muscarinic M3 Receptors Reveal Single
... reflect integration of signaling by two or more different receptors activated coincidentally or sequentially. In addition to heterologous desensitization, there are examples in which receptor activation either reveals or potentiates signaling by a different receptor type, although this is perhaps le ...
... reflect integration of signaling by two or more different receptors activated coincidentally or sequentially. In addition to heterologous desensitization, there are examples in which receptor activation either reveals or potentiates signaling by a different receptor type, although this is perhaps le ...
Regulation of KNOLLE syntaxin - Journal of Cell Science
... benzyl aminopurine (Sigma) pH 5.7 with KOH, and 0.005% SILWET L77 (Osi Specialities). T1 seeds were bulk-harvested (each seed representing a single transformation event; Bechtold et al., 2000; Desfeux et al., 2000; Ye et al., 1999), sown on soil and selected for transformants by spraying BASTA® (183 ...
... benzyl aminopurine (Sigma) pH 5.7 with KOH, and 0.005% SILWET L77 (Osi Specialities). T1 seeds were bulk-harvested (each seed representing a single transformation event; Bechtold et al., 2000; Desfeux et al., 2000; Ye et al., 1999), sown on soil and selected for transformants by spraying BASTA® (183 ...
vilnius university
... response have been introduced into clinical practice (2). Current antitumor immunotherapy strategies are designed to activate immune response, but taking into mind that malignancy may be associated with expansion of immunosuppressive components (3), it is likely that in such cases activation of the ...
... response have been introduced into clinical practice (2). Current antitumor immunotherapy strategies are designed to activate immune response, but taking into mind that malignancy may be associated with expansion of immunosuppressive components (3), it is likely that in such cases activation of the ...
Combinatorial Gli gene function in floor plate and
... (f) cDNAs. Plasmids expressing hGli1, hGli3 and fGli1 were as described previously (Lee et al., 1997). Plasmids driving the expression of fShh were as described in Ruiz i Altaba et al. (1995). The hGli1-hGli3 chimeric construct was made by replacing the Nterminal region (bp 1-1248) of hGli3 with a 7 ...
... (f) cDNAs. Plasmids expressing hGli1, hGli3 and fGli1 were as described previously (Lee et al., 1997). Plasmids driving the expression of fShh were as described in Ruiz i Altaba et al. (1995). The hGli1-hGli3 chimeric construct was made by replacing the Nterminal region (bp 1-1248) of hGli3 with a 7 ...
Document
... of hibernating grizzly bears using high-throughput proteomics and RNA-Seq. The changes in protein expression indicate an effect on muscle metabolism, consistent with increased nonessential amino acid levels and a decrease in ATP production. Supplementing murine myotubes with non-essential amino acid ...
... of hibernating grizzly bears using high-throughput proteomics and RNA-Seq. The changes in protein expression indicate an effect on muscle metabolism, consistent with increased nonessential amino acid levels and a decrease in ATP production. Supplementing murine myotubes with non-essential amino acid ...
Review
... simply to buffer or lower Ca2+ levels, and in others to trigger cellular processes. The local nature of Ca2+ signaling is intimately tied to this large range of affinities. The calcium ion can accommodate 4–12 oxygen atoms in its primary coordination sphere, with 6–8 being most common. The chelating ...
... simply to buffer or lower Ca2+ levels, and in others to trigger cellular processes. The local nature of Ca2+ signaling is intimately tied to this large range of affinities. The calcium ion can accommodate 4–12 oxygen atoms in its primary coordination sphere, with 6–8 being most common. The chelating ...
PDF
... of Gb’ft mRNA at 2 dpa was reduced (0.40±0.06), whereas that of Gb’ds was slightly increased (1.38±0.31). Similarly, in regenerating Gb’ds RNAi tibiae (n=10), the ratio of Gb’ds mRNA was reduced (0.37±0.12), whereas that of Gb’ft was slightly increased (1.28±0.36). In regenerating Gb’fj RNAi tibiae ...
... of Gb’ft mRNA at 2 dpa was reduced (0.40±0.06), whereas that of Gb’ds was slightly increased (1.38±0.31). Similarly, in regenerating Gb’ds RNAi tibiae (n=10), the ratio of Gb’ds mRNA was reduced (0.37±0.12), whereas that of Gb’ft was slightly increased (1.28±0.36). In regenerating Gb’fj RNAi tibiae ...
Regulation of leg size and shape by the Dachsous
... of Gb’ft mRNA at 2 dpa was reduced (0.40±0.06), whereas that of Gb’ds was slightly increased (1.38±0.31). Similarly, in regenerating Gb’ds RNAi tibiae (n=10), the ratio of Gb’ds mRNA was reduced (0.37±0.12), whereas that of Gb’ft was slightly increased (1.28±0.36). In regenerating Gb’fj RNAi tibiae ...
... of Gb’ft mRNA at 2 dpa was reduced (0.40±0.06), whereas that of Gb’ds was slightly increased (1.38±0.31). Similarly, in regenerating Gb’ds RNAi tibiae (n=10), the ratio of Gb’ds mRNA was reduced (0.37±0.12), whereas that of Gb’ft was slightly increased (1.28±0.36). In regenerating Gb’fj RNAi tibiae ...
Electron Tomographic Analysis of Somatic Cell
... spatial relationships between ER cisternae and the different cell plate membrane networks. For technical reasons, the phragmoplast-associated actin filaments, which also play a critical role in cell plate formation (Molchan et al., 2002), have not been reconstructed and are therefore not shown in ou ...
... spatial relationships between ER cisternae and the different cell plate membrane networks. For technical reasons, the phragmoplast-associated actin filaments, which also play a critical role in cell plate formation (Molchan et al., 2002), have not been reconstructed and are therefore not shown in ou ...
... protein-protein interaction, but two LIM domains in CRP1 have been shown to be independent units [26,29,30]. The CRP family is unique in that a glycine-rich region is located adjacent to each LIM domain [23,24]. Also, CRP members have two distinct locations in a cell: nucleus and actin cytoskeleton. ...
Electron Tomographic Analysis of Somatic Cell Plate Formation in
... spatial relationships between ER cisternae and the different cell plate membrane networks. For technical reasons, the phragmoplast-associated actin filaments, which also play a critical role in cell plate formation (Molchan et al., 2002), have not been reconstructed and are therefore not shown in ou ...
... spatial relationships between ER cisternae and the different cell plate membrane networks. For technical reasons, the phragmoplast-associated actin filaments, which also play a critical role in cell plate formation (Molchan et al., 2002), have not been reconstructed and are therefore not shown in ou ...
In Vitro Reconstitution of SCF Substrate Ubiquitination with Purified
... approximately 2 g is shown. (C) SCFCdc4 was expressed in Hi5 insect cells by coinfection of baculoviruses encoding Py3HA2‐tagged Cdc4, Cdc53, HA‐tagged Skp1, and Hrt1. Forty‐eight hours postinfection, lysates were prepared and SCFCdc4 was purified on protein A‐Sepharose beads that had been coupled ...
... approximately 2 g is shown. (C) SCFCdc4 was expressed in Hi5 insect cells by coinfection of baculoviruses encoding Py3HA2‐tagged Cdc4, Cdc53, HA‐tagged Skp1, and Hrt1. Forty‐eight hours postinfection, lysates were prepared and SCFCdc4 was purified on protein A‐Sepharose beads that had been coupled ...
Out of the Mouths of Plants: The Molecular Basis of the Evolution
... cell–cell signals in turn appear to activate a mitogen-activated protein (MAP) kinase signaling cascade including the MAPKKK YODA (Bergmann et al., 2004), MPKK4/5, and MAPK MPK3/6 (Wang et al., 2007), to suppress stomatal development in neighboring cells. In general, MAP kinase cascades target trans ...
... cell–cell signals in turn appear to activate a mitogen-activated protein (MAP) kinase signaling cascade including the MAPKKK YODA (Bergmann et al., 2004), MPKK4/5, and MAPK MPK3/6 (Wang et al., 2007), to suppress stomatal development in neighboring cells. In general, MAP kinase cascades target trans ...
Free ISG15 as a dimer generates IL-1β-producing CD8α+
... Similar to ubiquitin, ISG15 can be conjugated to intracellular host and viral proteinsemploying a cascade of E1 (UBE1L), E2 (UbcH8), and E3 conjugating enzymes. This ISGylation can modify protein function (LIT) and extensive studies have elucidated the role conjugated ISG15, mostly in the context of ...
... Similar to ubiquitin, ISG15 can be conjugated to intracellular host and viral proteinsemploying a cascade of E1 (UBE1L), E2 (UbcH8), and E3 conjugating enzymes. This ISGylation can modify protein function (LIT) and extensive studies have elucidated the role conjugated ISG15, mostly in the context of ...
PLEKHM1/DEF8/RAB7 complex regulates lysosome positioning and
... Osteoclasts are multinucleated cells that are capable of resorbing calcified cartilage and bone matrix during skeletal development, homeostasis, and repair (1). Increased osteoclast number or activity leads to bone loss in metabolic bone diseases, such as postmenopausal osteoporosis (2). Conversely, ...
... Osteoclasts are multinucleated cells that are capable of resorbing calcified cartilage and bone matrix during skeletal development, homeostasis, and repair (1). Increased osteoclast number or activity leads to bone loss in metabolic bone diseases, such as postmenopausal osteoporosis (2). Conversely, ...
Here - Events
... pauses cell cycle progression to partition the nuclei into individual cells and to assign those cells distinct developmental fates. The pause in cell cycle is analogous to the midblastula transition observed in many organisms and is associated with the degradation of a subset of maternally supplied ...
... pauses cell cycle progression to partition the nuclei into individual cells and to assign those cells distinct developmental fates. The pause in cell cycle is analogous to the midblastula transition observed in many organisms and is associated with the degradation of a subset of maternally supplied ...
Biochemical characterization of the vanilloid receptor 1 expressed in
... transfection efficiency was about 70% for the HEK293 cells and about 50% for the F-11 cells, as assessed by indirect immunofluorescence (see below). Both in lysates from transiently transfected HEK293 and F-11 cells, the anti-VR1 serum specifically detected a diffuse protein band at < 115 kDa and a ...
... transfection efficiency was about 70% for the HEK293 cells and about 50% for the F-11 cells, as assessed by indirect immunofluorescence (see below). Both in lysates from transiently transfected HEK293 and F-11 cells, the anti-VR1 serum specifically detected a diffuse protein band at < 115 kDa and a ...
The Molecular Basis of the Evolution and Diversity of Stomatal
... cell–cell signals in turn appear to activate a mitogen-activated protein (MAP) kinase signaling cascade including the MAPKKK YODA (Bergmann et al., 2004), MPKK4/5, and MAPK MPK3/6 (Wang et al., 2007), to suppress stomatal development in neighboring cells. In general, MAP kinase cascades target trans ...
... cell–cell signals in turn appear to activate a mitogen-activated protein (MAP) kinase signaling cascade including the MAPKKK YODA (Bergmann et al., 2004), MPKK4/5, and MAPK MPK3/6 (Wang et al., 2007), to suppress stomatal development in neighboring cells. In general, MAP kinase cascades target trans ...
Golgi clusters and vesicles mediate mitotic inheritance
... Distinction between Golgi apparatus and ER in mitotic normal rat kidney (NRK) cells Our earlier studies using a GFP-tagged Golgi resident showed that clusters could be visualized at all stages of mitosis. At no stage were they observed to disappear. These experiments were carried out using a HeLa ce ...
... Distinction between Golgi apparatus and ER in mitotic normal rat kidney (NRK) cells Our earlier studies using a GFP-tagged Golgi resident showed that clusters could be visualized at all stages of mitosis. At no stage were they observed to disappear. These experiments were carried out using a HeLa ce ...