Molecular architecture of the pyruvate dehydrogenase complex
... a second molecule of E2-SBD with either E1 or E3 is not possible. Occupation of both binding sites would result in steric clashes in one of the E2-SBD loop regions. In contrast, it has been known for a long time that a total of 30 E1 and six E3 molecules can associate with the mammalian PDC core [22 ...
... a second molecule of E2-SBD with either E1 or E3 is not possible. Occupation of both binding sites would result in steric clashes in one of the E2-SBD loop regions. In contrast, it has been known for a long time that a total of 30 E1 and six E3 molecules can associate with the mammalian PDC core [22 ...
Systemic methods for capturing protein–lipid interactions (PDF
... • 25% of the identified lipid interaction proteome is enriched in drug targets, while 12% of total human proteome is drugged. lipid probes may preferentially interact with proteins that can bind other small molecule ligands ...
... • 25% of the identified lipid interaction proteome is enriched in drug targets, while 12% of total human proteome is drugged. lipid probes may preferentially interact with proteins that can bind other small molecule ligands ...
Traffic into silence: endomembranes and
... trans-Golgi network (TGN), endosomes, and lysosomes or vacuoles. Flows of membranes and proteins to (outward) and from (inward) the plasma membrane go through these organelles. Endosomes sit at an essential position within the endomembrane system and have a core sorting function: They are the first ...
... trans-Golgi network (TGN), endosomes, and lysosomes or vacuoles. Flows of membranes and proteins to (outward) and from (inward) the plasma membrane go through these organelles. Endosomes sit at an essential position within the endomembrane system and have a core sorting function: They are the first ...
Amyloid Deposits: Protection Against Toxic Protein
... plaques, also referred to as classic or cored plaques, contain a dense amyloid core surrounded in turn by a ring of abnormal cellular processes and a rim of diffuse amyloid11, 12. In these neuritic plaques tau accumulation can also be present in dystrophic neurites surrounding the amyloid core. It h ...
... plaques, also referred to as classic or cored plaques, contain a dense amyloid core surrounded in turn by a ring of abnormal cellular processes and a rim of diffuse amyloid11, 12. In these neuritic plaques tau accumulation can also be present in dystrophic neurites surrounding the amyloid core. It h ...
PDF - Herbert Publications
... antigen-binding region of such antibodies), although, in contrast to proteins, they have nothing to do with genetic information. 2. Sugars constitute the immunodominant regions of many natural antigens that contain a protein component as well (glycoproteins). 3. Many strong natural antigens have no ...
... antigen-binding region of such antibodies), although, in contrast to proteins, they have nothing to do with genetic information. 2. Sugars constitute the immunodominant regions of many natural antigens that contain a protein component as well (glycoproteins). 3. Many strong natural antigens have no ...
Dissection of autophagy in tobacco BY-2 cells
... cells, the pH of the central vacuoles is raised by treatment with bafilomycin and concanamycin.31,32 In tobacco BY-2 cells, concanamycin inhibits the transport of vacuolar resident proteins to the central vacuole, although it does not seem to affect the pH of central vacuoles at the concentrations u ...
... cells, the pH of the central vacuoles is raised by treatment with bafilomycin and concanamycin.31,32 In tobacco BY-2 cells, concanamycin inhibits the transport of vacuolar resident proteins to the central vacuole, although it does not seem to affect the pH of central vacuoles at the concentrations u ...
Isolation and Characterization of Piscine Osteonectin and
... Sparus auratus. It is expressed by cells forming the basement membrane of calcifying tissue indicating that, as in mammals, it may play a role in osteoblast differentiation. PTHrP induced downregulation of osteonectin mRNA in vitro in scales, a mineralizing tissue with bone-like metabolism. This ind ...
... Sparus auratus. It is expressed by cells forming the basement membrane of calcifying tissue indicating that, as in mammals, it may play a role in osteoblast differentiation. PTHrP induced downregulation of osteonectin mRNA in vitro in scales, a mineralizing tissue with bone-like metabolism. This ind ...
M6PRs are found in a subset of PC12 cell ISGs
... Carrier-free [35S]sulfate and 125I-Protein A were from AmershamPharmacia Biotech, UK. Nucleotides, creatine phosphate, and creatine phosphokinase were from Boehringer Mannheim, Germany. Casein kinase II was prepared according to the method of Litchfield et al. (1990). Heparin and horseradish peroxid ...
... Carrier-free [35S]sulfate and 125I-Protein A were from AmershamPharmacia Biotech, UK. Nucleotides, creatine phosphate, and creatine phosphokinase were from Boehringer Mannheim, Germany. Casein kinase II was prepared according to the method of Litchfield et al. (1990). Heparin and horseradish peroxid ...
Control of the Cell Cycle in Early Embryos
... for the duration of this experiment (20 min), and disappeared at anaphase of meiosis I (about 30 rain), never reappearing during subsequent meiotic or mitotic divisions (not shown). The speed of this response and the fact that many cell-surface receptors contain tyrosine kinase activities or can act ...
... for the duration of this experiment (20 min), and disappeared at anaphase of meiosis I (about 30 rain), never reappearing during subsequent meiotic or mitotic divisions (not shown). The speed of this response and the fact that many cell-surface receptors contain tyrosine kinase activities or can act ...
Document
... • Most mutations confer sensitivity, not resistance to a specific condition • Screening for resistance is easy, simply selecting will do ...
... • Most mutations confer sensitivity, not resistance to a specific condition • Screening for resistance is easy, simply selecting will do ...
Chapter 14 PowerPoint Slides PDF - CM
... • Effects on cells of adrenal medulla: adrenal medulla sits on top of each kidney; in direct contact with preganglionic sympathetic neurons; medulla is composed of modified sympathetic postganglionic neurons with following functions (Figure 14.8): ...
... • Effects on cells of adrenal medulla: adrenal medulla sits on top of each kidney; in direct contact with preganglionic sympathetic neurons; medulla is composed of modified sympathetic postganglionic neurons with following functions (Figure 14.8): ...
Origin and evolution of peptide-modifying
... collagen-like proteins in bacteria. Jumonji-related domains diversified into three distinct lineages in bacterial secondary metabolism systems and these were precursors of the three major clades of ...
... collagen-like proteins in bacteria. Jumonji-related domains diversified into three distinct lineages in bacterial secondary metabolism systems and these were precursors of the three major clades of ...
Bound by Fate: The Role of Reactive Oxygen
... RBOHs are synergistically regulated by phosphorylation and calcium (Ca2+) binding to EF-hand domains in the N-terminal cytosolic region (Kärkönen and Kuchitsu, 2015). While RLKs might stimulate extracellular ROS production via several mechanisms, genetic and biochemical analyses have highlighted two ...
... RBOHs are synergistically regulated by phosphorylation and calcium (Ca2+) binding to EF-hand domains in the N-terminal cytosolic region (Kärkönen and Kuchitsu, 2015). While RLKs might stimulate extracellular ROS production via several mechanisms, genetic and biochemical analyses have highlighted two ...
Mechanism of high-mobility group protein B enhancement of
... mutagenesis to be essential for binding of PR and ER to half-site HREs (24,25). It has also been reported that acetylation of two lysine residues in the CTE of ER-a enhanced DNA binding and transcriptional activity (26). Thus the CTE of ER and PR participate directly in DNA binding and appear to be ...
... mutagenesis to be essential for binding of PR and ER to half-site HREs (24,25). It has also been reported that acetylation of two lysine residues in the CTE of ER-a enhanced DNA binding and transcriptional activity (26). Thus the CTE of ER and PR participate directly in DNA binding and appear to be ...
Terminal Cytokinesis Events Uncovered after an RNAi Screen
... separation of daughter cells. Rather, as first described by Flemming in 1891 (see [7]), a persistent intercellular bridge forms around the spindle remnant; this bridge is marked at its center by a darkly staining structure, or midbody (e.g., Figure 1A, insert). This bridge remains long after furrowi ...
... separation of daughter cells. Rather, as first described by Flemming in 1891 (see [7]), a persistent intercellular bridge forms around the spindle remnant; this bridge is marked at its center by a darkly staining structure, or midbody (e.g., Figure 1A, insert). This bridge remains long after furrowi ...
Mob4 plays a role in spindle focusing in Drosophila S2 cells
... (G) Mob4-RNAi-treated cell with two centrosomes forming a bipolar spindle that becomes unorganized at both poles owing to detachment of K fibers from the centrosome and splaying during prometaphase/metaphase. (Full time-lapse movie available in supplementary material Movie 3.) Anaphase onset in Mob4 ...
... (G) Mob4-RNAi-treated cell with two centrosomes forming a bipolar spindle that becomes unorganized at both poles owing to detachment of K fibers from the centrosome and splaying during prometaphase/metaphase. (Full time-lapse movie available in supplementary material Movie 3.) Anaphase onset in Mob4 ...
2013 Action Potential Modeling in PYTHON
... excitable cells because the underlying mechanisms of ion transport do not change[8]. The use of this programming language enhances the accessibility of this model for researchers in different fields of science due to good code readability and the simplicity in modifying the code[8]. Some future res ...
... excitable cells because the underlying mechanisms of ion transport do not change[8]. The use of this programming language enhances the accessibility of this model for researchers in different fields of science due to good code readability and the simplicity in modifying the code[8]. Some future res ...
BE WHEN THEY GROW UP? Lessons from Epidermal Patterning in
... suppress the mutant defects of ttg when constitutively expressed in Arabidopsis, as well as cause ectopic production of trichomes, especially when coexpressed with GL1, but until the identification of GL3, no Arabidopsis gene encoding a bHLH protein had been implicated in trichome development (45, 5 ...
... suppress the mutant defects of ttg when constitutively expressed in Arabidopsis, as well as cause ectopic production of trichomes, especially when coexpressed with GL1, but until the identification of GL3, no Arabidopsis gene encoding a bHLH protein had been implicated in trichome development (45, 5 ...
Corresponding author: Dr. A. Lacey Samuels
... breakdown products were consistently shown to be radioactive, indicating incorporation of 3H-phenylalanine into the lignin polymer (Supplemental Figure 1). To investigate the nature of the soluble metabolites that were labeled during the four hour incubation with 3H-phenylalanine, methanol soluble p ...
... breakdown products were consistently shown to be radioactive, indicating incorporation of 3H-phenylalanine into the lignin polymer (Supplemental Figure 1). To investigate the nature of the soluble metabolites that were labeled during the four hour incubation with 3H-phenylalanine, methanol soluble p ...
PDF Document
... activated ASIC2a channels from ~25% at 0.1 mM MOPS to ~2% at 5 mM MOPS (Fig. 2d). In contrast, the extracellular bulk volume exhibited lesser importance; consecutive exchange of the bulk medium during illumination by continuous perfusion only slightly decreased the light activated ASIC2a current com ...
... activated ASIC2a channels from ~25% at 0.1 mM MOPS to ~2% at 5 mM MOPS (Fig. 2d). In contrast, the extracellular bulk volume exhibited lesser importance; consecutive exchange of the bulk medium during illumination by continuous perfusion only slightly decreased the light activated ASIC2a current com ...
Inhibition of Phorbol Ester-Induced Monocytic
... gene shares homology with other tyrosine kinase-encoding protooncogenes.” Moreover, the c-fms product is identical to the CSF-1 receptor.’ These findings have suggested that CSF-1 is capable of regulating monocyte functions by an autocrine mechanism. The c-fms transcripts become detectable a t 12 to ...
... gene shares homology with other tyrosine kinase-encoding protooncogenes.” Moreover, the c-fms product is identical to the CSF-1 receptor.’ These findings have suggested that CSF-1 is capable of regulating monocyte functions by an autocrine mechanism. The c-fms transcripts become detectable a t 12 to ...
Intro to Cell Biology - Mrs. Florio's Science Class
... ACIDS used by cells ____________ to ________________ make proteins ...
... ACIDS used by cells ____________ to ________________ make proteins ...
Signal transduction
Signal transduction occurs when an extracellular signaling molecule activates a specific receptor located on the cell surface or inside the cell. In turn, this receptor triggers a biochemical chain of events inside the cell, creating a response. Depending on the cell, the response alters the cell's metabolism, shape, gene expression, or ability to divide. The signal can be amplified at any step. Thus, one signaling molecule can cause many responses.