Chapter 1 The Foundations of Biochemistry
... use energy from their environment; (3) have the capacity to precisely self-replicate and self-assemble; (4) exploit a chemical interplay with their environment; (5) possess programmatically defined functions; and (6) evolve to new forms over many generations. 26. Cellular foundations Page: 3 Difficu ...
... use energy from their environment; (3) have the capacity to precisely self-replicate and self-assemble; (4) exploit a chemical interplay with their environment; (5) possess programmatically defined functions; and (6) evolve to new forms over many generations. 26. Cellular foundations Page: 3 Difficu ...
The Sad1-UNC-84 homology domain in Mps3 interacts with Mps2 to
... the half-bridge, which is important for SPB duplication as well as for microtubule nucleation during G1 (Byers and Goetsch, 1974, 1975). SPBs in fission yeast have a similar but not identical structure. Importantly, the S. pombe SPB is also embedded in the nuclear envelope, possibly by its SUN prote ...
... the half-bridge, which is important for SPB duplication as well as for microtubule nucleation during G1 (Byers and Goetsch, 1974, 1975). SPBs in fission yeast have a similar but not identical structure. Importantly, the S. pombe SPB is also embedded in the nuclear envelope, possibly by its SUN prote ...
Signals and circuits in the Purkinje neuron NEURAL CIRCUITS Ze’ev R. Abrams
... analysis. The terms “signal” and “circuit” have particular meanings in EE that are not always carried over when used in the field of neuroscience. The most elementary methodology to reverse engineer the PN, whose functions are unknown, is to treat it as a “black box,” and measure the response of the ...
... analysis. The terms “signal” and “circuit” have particular meanings in EE that are not always carried over when used in the field of neuroscience. The most elementary methodology to reverse engineer the PN, whose functions are unknown, is to treat it as a “black box,” and measure the response of the ...
Break of symmetry in regenerating tobacco protoplasts is
... Linda Brochhausen*, Jan Maisch and Peter Nick Molecular Cell Biology, Botanical Institute, Karlsruhe Institute of Technology, Kaiserstr. 2, D-76133 Karlsruhe, Germany. *Correspondence: Linda.Aimee@Brochhausen.com ...
... Linda Brochhausen*, Jan Maisch and Peter Nick Molecular Cell Biology, Botanical Institute, Karlsruhe Institute of Technology, Kaiserstr. 2, D-76133 Karlsruhe, Germany. *Correspondence: Linda.Aimee@Brochhausen.com ...
Osmoregulation in Halophilic Bacteria
... the cell (as proven for Haloferax volcanii through an energy-dependent potassium uptake system) and, together with its counterion Cl–, K+ can be found in molar concentrations in the cytoplasm. Because the K+ concentration inside the cell is 100 times higher than in the surrounding environment, a par ...
... the cell (as proven for Haloferax volcanii through an energy-dependent potassium uptake system) and, together with its counterion Cl–, K+ can be found in molar concentrations in the cytoplasm. Because the K+ concentration inside the cell is 100 times higher than in the surrounding environment, a par ...
Facultad de Ciencias Tráfico de células dendríticas a través
... until the thoracic ducts, where lymph is poured into the blood vascular system through subclavian veins. In order to accomplish its draining function, the lymphatic capillaries form an intricate network inside the tissue, in close contact with blood vessels, but without anastomose with them. The mai ...
... until the thoracic ducts, where lymph is poured into the blood vascular system through subclavian veins. In order to accomplish its draining function, the lymphatic capillaries form an intricate network inside the tissue, in close contact with blood vessels, but without anastomose with them. The mai ...
Lack of response suppression follows repeated ventral tegmental
... presynaptic terminals of hippocampal interneurons supports this hypothesis. Nevertheless, autoradiographic and immunohistochemistry studies have shown that CB1 receptor levels are sparse within midbrain structures such as the VTA, possibly indicating a neuromodulatory role of the receptors. 13,25 Th ...
... presynaptic terminals of hippocampal interneurons supports this hypothesis. Nevertheless, autoradiographic and immunohistochemistry studies have shown that CB1 receptor levels are sparse within midbrain structures such as the VTA, possibly indicating a neuromodulatory role of the receptors. 13,25 Th ...
212_spring_2005_Platelets
... which down regulate the signalling pathways that support platelet activation ...
... which down regulate the signalling pathways that support platelet activation ...
Bound by Fate: Reactive Oxygen Species in
... responses to abiotic cues (Marshall et al., 2012). Since plants are constantly exposed to multiple stimuli, the large number of RLKs and the corresponding potential ligands might mediate the integration of simultaneous signals through crosstalk and the use of similar signaling components. Despite th ...
... responses to abiotic cues (Marshall et al., 2012). Since plants are constantly exposed to multiple stimuli, the large number of RLKs and the corresponding potential ligands might mediate the integration of simultaneous signals through crosstalk and the use of similar signaling components. Despite th ...
Escherichia coli Karl Skoog
... the replication of the DNA, followed by an elongation step in which the cells become twice as long. In the last step the elongated cell constricts in the middle and the two daughter cells are separated. The cell division process in E. coli has been extensively studied for at least 50 years and a lot ...
... the replication of the DNA, followed by an elongation step in which the cells become twice as long. In the last step the elongated cell constricts in the middle and the two daughter cells are separated. The cell division process in E. coli has been extensively studied for at least 50 years and a lot ...
Female Gametophyte Development in Flowering Plants
... the megasporocyte is manifested by the polar distribution of organelles, the dynamic deposition of callose, and the microtubule cytoskeleton. So far, the role of this polarity on megaspore development is unknown. The disruption of this polarity in megasporocytes was observed in switch1 (swi1)/dyad o ...
... the megasporocyte is manifested by the polar distribution of organelles, the dynamic deposition of callose, and the microtubule cytoskeleton. So far, the role of this polarity on megaspore development is unknown. The disruption of this polarity in megasporocytes was observed in switch1 (swi1)/dyad o ...
Induction of a Secondary Body Axis in Xenopus by Antibodies to
... ot-Catenin may fulfill the role of a cytoskeletal linker protein. It shares overall structural similarity and primary amino acid sequence homology with vinculin (14, 27), a protein known to be involved in actin filament attachment to the plasma membrane at sites of adhesive junctions (2, 9, 33). Als ...
... ot-Catenin may fulfill the role of a cytoskeletal linker protein. It shares overall structural similarity and primary amino acid sequence homology with vinculin (14, 27), a protein known to be involved in actin filament attachment to the plasma membrane at sites of adhesive junctions (2, 9, 33). Als ...
Recombinant DNA procedures for producing small antimicrobial
... chemical methods (Table I). Three different fusion protein expression systems were tried in preliminary studies, involving fusions to GST on plasmid pGEX-KP [a derivative of pGEX-3X (Pharmacia) in which the BumHISmuI-EcoRI multiple cloning site was changed to SphIHindIII-EcoRI by PCR], to the N-term ...
... chemical methods (Table I). Three different fusion protein expression systems were tried in preliminary studies, involving fusions to GST on plasmid pGEX-KP [a derivative of pGEX-3X (Pharmacia) in which the BumHISmuI-EcoRI multiple cloning site was changed to SphIHindIII-EcoRI by PCR], to the N-term ...
TIBS Review - Structural Genomics Consortium
... BRDT has a central role in chromatin remodelling during spermatogenesis as indicated by the importance of its first bromodomain in maintaining chromatin architecture of the sperm head [50]. A recent study delineated BRDT’s role in re-organizing chromatin, facilitating histone eviction and replacemen ...
... BRDT has a central role in chromatin remodelling during spermatogenesis as indicated by the importance of its first bromodomain in maintaining chromatin architecture of the sperm head [50]. A recent study delineated BRDT’s role in re-organizing chromatin, facilitating histone eviction and replacemen ...
Microtubule-Associated Protein 1B
... generated previously as described in Magiera et al. (2004). GSTfusions of the CAT domain (amino acids 619–881), CAMP domain (amino acids 199–316), Dishevelled, Egl-10, and pleckstrin (DEP) domain (amino acids 74–140), and Ras exchange motif (REM) domain (amino acids 345–410) were then purified using ...
... generated previously as described in Magiera et al. (2004). GSTfusions of the CAT domain (amino acids 619–881), CAMP domain (amino acids 199–316), Dishevelled, Egl-10, and pleckstrin (DEP) domain (amino acids 74–140), and Ras exchange motif (REM) domain (amino acids 345–410) were then purified using ...
Insulin mRNA to Protein Kit Student Handout
... near the 5’-end of the mRNA and begins translating the protein, starting with methionine. • The ribosome synthesizes a precursor form of insulin, known as preproinsulin. • Preproinsulin is processed to become mature, functional insulin as it proceeds through the endoplasmic reticulum and Golgi app ...
... near the 5’-end of the mRNA and begins translating the protein, starting with methionine. • The ribosome synthesizes a precursor form of insulin, known as preproinsulin. • Preproinsulin is processed to become mature, functional insulin as it proceeds through the endoplasmic reticulum and Golgi app ...
Genome-wide analysis of the sox family in the calcareous
... Methods: Calcaronean Sox related sequences were retrieved by searching recently generated genomic and transcriptome sequence resources and analyzed using variety of phylogenetic methods and identification of conserved motifs. Expression was studied by whole mount in situ hybridization. Results: We h ...
... Methods: Calcaronean Sox related sequences were retrieved by searching recently generated genomic and transcriptome sequence resources and analyzed using variety of phylogenetic methods and identification of conserved motifs. Expression was studied by whole mount in situ hybridization. Results: We h ...
aud
... that of a sugar cube (see Figure 4-3). The middle ear communicates with the nasopharynx via the Eustachian tube. This tube is approximately 35 mm in length in adults and angles downward and forward to connect the anterior wall of the tympanic cavity with the nasopharynx. The tube is normally closed, ...
... that of a sugar cube (see Figure 4-3). The middle ear communicates with the nasopharynx via the Eustachian tube. This tube is approximately 35 mm in length in adults and angles downward and forward to connect the anterior wall of the tympanic cavity with the nasopharynx. The tube is normally closed, ...
Magnificent molecules
... But these diamines are not just about corpses. They are produced in normal living tissue as well, where they help the process of cell division. The cadaverine and putrescine that our own, living bodies produce contribute a little to the smell of urine. ...
... But these diamines are not just about corpses. They are produced in normal living tissue as well, where they help the process of cell division. The cadaverine and putrescine that our own, living bodies produce contribute a little to the smell of urine. ...
In cellulo Evaluation of Phototransformation Quantum - HAL
... occurrence of long-lasting dark states has been noticed [26,27,28], but no quantum yields for the corresponding on-off transitions have been reported. Long-lasting dark states of PTFPs deserve special attention because they are detrimental to PALM microscopy and single-particle tracking experiments. ...
... occurrence of long-lasting dark states has been noticed [26,27,28], but no quantum yields for the corresponding on-off transitions have been reported. Long-lasting dark states of PTFPs deserve special attention because they are detrimental to PALM microscopy and single-particle tracking experiments. ...
SUPPLEMENTAL DATA FOR DUPLICATED SACCHAROMYCES
... - G121D: Asp side chain is possibly too far to form a hydrogen bond to FMN. S3.3. Cellular localization There are conflicting reports for the location of PST2 and RFS1. In earlier studies, the green fluorescent protein (GFP)-fusion protein of PST2 localized to the cytoplasm in a punctuate pattern [5 ...
... - G121D: Asp side chain is possibly too far to form a hydrogen bond to FMN. S3.3. Cellular localization There are conflicting reports for the location of PST2 and RFS1. In earlier studies, the green fluorescent protein (GFP)-fusion protein of PST2 localized to the cytoplasm in a punctuate pattern [5 ...
Synaptonemal complex proteins - Journal of Cell Science
... To determine whether the Syn1 protein might display DNA binding activity, a western blot suitable for Southwestern analysis was prepared from an SDS-PAGE gel on which insoluble protein from E. coli expressing SYN1c, SYN1d and GST had been electrophoresed. The transfer was incubated with a 0.4 kb [32 ...
... To determine whether the Syn1 protein might display DNA binding activity, a western blot suitable for Southwestern analysis was prepared from an SDS-PAGE gel on which insoluble protein from E. coli expressing SYN1c, SYN1d and GST had been electrophoresed. The transfer was incubated with a 0.4 kb [32 ...
the role of csf1 and zfpm1 in the preimplantation mouse
... remain as pluripotent epiblast (EPI) cells. These two important events are triggered by several factors including an individual cell´s relative position within the embryo, the development of embryonic and cellular polarity, differential gene expression, as well as cell signaling and epigenetic modif ...
... remain as pluripotent epiblast (EPI) cells. These two important events are triggered by several factors including an individual cell´s relative position within the embryo, the development of embryonic and cellular polarity, differential gene expression, as well as cell signaling and epigenetic modif ...
Two Genes with Similarity to Bacterial Response Regulators Are
... present in IBC6. IBC7 also contains two potential myristylation sites near the N terminus. A cDNA clone (RIC2168A) was identified in the rice EST database whose sequence is 69% identical and 95% similar to the IBC6 amino acid sequence (Figure 1C). This rice gene also possesses a C-terminal extension ...
... present in IBC6. IBC7 also contains two potential myristylation sites near the N terminus. A cDNA clone (RIC2168A) was identified in the rice EST database whose sequence is 69% identical and 95% similar to the IBC6 amino acid sequence (Figure 1C). This rice gene also possesses a C-terminal extension ...
Experimental and genetic analysis of root development in
... ablated cells and resulted in cytoplasmic staining (Figure 3B). The induced permeability was not transient. If propidium iodine was again added after growing the roots for a prolonged time without the dye, the ablated cells could be re-stained (Figure 3C). Without new addition of dye no CLSM image w ...
... ablated cells and resulted in cytoplasmic staining (Figure 3B). The induced permeability was not transient. If propidium iodine was again added after growing the roots for a prolonged time without the dye, the ablated cells could be re-stained (Figure 3C). Without new addition of dye no CLSM image w ...
Signal transduction
Signal transduction occurs when an extracellular signaling molecule activates a specific receptor located on the cell surface or inside the cell. In turn, this receptor triggers a biochemical chain of events inside the cell, creating a response. Depending on the cell, the response alters the cell's metabolism, shape, gene expression, or ability to divide. The signal can be amplified at any step. Thus, one signaling molecule can cause many responses.