Ghorbaniaghdam (oral)
... Monoclonal antibody producing Chinese hamster ovary (CHO) cells have been shown to undergo metabolic changes when engineered to produce high titers of recombinant proteins. In this work, we have studied the distinct metabolism of CHO cell clones harboring an efficient inducible expression system, ba ...
... Monoclonal antibody producing Chinese hamster ovary (CHO) cells have been shown to undergo metabolic changes when engineered to produce high titers of recombinant proteins. In this work, we have studied the distinct metabolism of CHO cell clones harboring an efficient inducible expression system, ba ...
May 21, 04copy.doc
... lasting disinhibition in neurons of deprived barrel columns, such as the increase in spontaneous activity, and the decrease in preference for angle of whisker deflection (Simons and Land, 1987). This respecification of receptive fields can also be mapped using markers for neuronal activity such as 2 ...
... lasting disinhibition in neurons of deprived barrel columns, such as the increase in spontaneous activity, and the decrease in preference for angle of whisker deflection (Simons and Land, 1987). This respecification of receptive fields can also be mapped using markers for neuronal activity such as 2 ...
Sequence-based predictions of membrane-protein topology, homology and insertion
... the cell is only partially shielded from the exterior, and this balance is actively regulated by membrane spanning channels, transporters and receptors. Thus, in response to external changes, channels open and close, energydriven pumps are activated and de-activated, and ligand-binding receptors giv ...
... the cell is only partially shielded from the exterior, and this balance is actively regulated by membrane spanning channels, transporters and receptors. Thus, in response to external changes, channels open and close, energydriven pumps are activated and de-activated, and ligand-binding receptors giv ...
Control of the number of cell division rounds in distinct tissues
... developmental ‘clock’ and ‘timer’ to specify mechanisms that measure embryonic time. The ‘clock’ is defined as a mechanism that can measure the period elapsed after fertilization, to which cells refer for the decision to cease dividing. When the clock measures the time elapsed after fertilization, w ...
... developmental ‘clock’ and ‘timer’ to specify mechanisms that measure embryonic time. The ‘clock’ is defined as a mechanism that can measure the period elapsed after fertilization, to which cells refer for the decision to cease dividing. When the clock measures the time elapsed after fertilization, w ...
Adaptative biochemical pathways and regulatory networks in
... pneumoniae and Salmonella typhimurium, can grow on citrate by a Na(I)-dependent pathway, forming acetic acid and CO2 as final metabolites [1, 8, 9]. Genes specific for anaerobic citrate fermentation, such as those coding for regulators (citAB), catabolic enzymes (citCDEFG and oadGAB) and citrate tra ...
... pneumoniae and Salmonella typhimurium, can grow on citrate by a Na(I)-dependent pathway, forming acetic acid and CO2 as final metabolites [1, 8, 9]. Genes specific for anaerobic citrate fermentation, such as those coding for regulators (citAB), catabolic enzymes (citCDEFG and oadGAB) and citrate tra ...
Vesicle Trafficking during Somatic Cytokinesis
... derived vesicles to the forming cell plate along the parallel-oriented microtubules of the phragmoplast by means of plus-end-directed microtubule motor proteins. Indeed, ultrastructural analysis has revealed a close association of vesicles with the phragmoplast microtubules (Kakimoto and Shibaoka, 1 ...
... derived vesicles to the forming cell plate along the parallel-oriented microtubules of the phragmoplast by means of plus-end-directed microtubule motor proteins. Indeed, ultrastructural analysis has revealed a close association of vesicles with the phragmoplast microtubules (Kakimoto and Shibaoka, 1 ...
Serotonin synchronises convergent extension of
... appears which are 5-ht2Dro-speci®c since they are pharmacologically indistinguishable from those of 5-ht2Dro transfected cells (Colas et al., 1995). As previously reported, concomitant with this expression, there is also a transient peak of 5-HT synthesis (Colas et al., 1995). The serotonin receptor ...
... appears which are 5-ht2Dro-speci®c since they are pharmacologically indistinguishable from those of 5-ht2Dro transfected cells (Colas et al., 1995). As previously reported, concomitant with this expression, there is also a transient peak of 5-HT synthesis (Colas et al., 1995). The serotonin receptor ...
Expression and Endocytosis of Lysosomal
... neither MRI nor the histopathological analysis have shown significant atrophy in the AGU brains (Haltia et al., 1975; Autti et al., 1997). Aspartylglucosaminidase is synthesized as an inactive precursor polypeptide, which is activated in the endoplasmic reticulum (ER) by dimerization followed by pro ...
... neither MRI nor the histopathological analysis have shown significant atrophy in the AGU brains (Haltia et al., 1975; Autti et al., 1997). Aspartylglucosaminidase is synthesized as an inactive precursor polypeptide, which is activated in the endoplasmic reticulum (ER) by dimerization followed by pro ...
Vesicle-mediated Protein Transport: Regulatory
... these vps (vacuolar protein sorting defective) mutants missort and secrete vacuolar proteins as their Golgi-modified precursors. Characterization of the products of the VPS genes has provided considerable insight into the molecules and mechanisms involved in the signal-mediated delivery of proteins ...
... these vps (vacuolar protein sorting defective) mutants missort and secrete vacuolar proteins as their Golgi-modified precursors. Characterization of the products of the VPS genes has provided considerable insight into the molecules and mechanisms involved in the signal-mediated delivery of proteins ...
rEvIEW - McLoon Lab
... release were found to be more likely to be ensheathed by astrocytes22. It is equally possible, however, that synapses that are wrapped by an astrocyte are stabilized and receive nurturing signals that allow them to mature further. It is important to point out that astrocyte processes do not fully in ...
... release were found to be more likely to be ensheathed by astrocytes22. It is equally possible, however, that synapses that are wrapped by an astrocyte are stabilized and receive nurturing signals that allow them to mature further. It is important to point out that astrocyte processes do not fully in ...
Novel Riboswitch Ligand Analogs as Selective Inhibitors of Guanine
... selection of chemical scaffolds, which makes it easier for bacterial pathogens to quickly develop resistance against new drug derivatives. This recurrent problem of multiple drug resistance implies a constant need to search for novel microbial targets and to modulate their activity using artificial ...
... selection of chemical scaffolds, which makes it easier for bacterial pathogens to quickly develop resistance against new drug derivatives. This recurrent problem of multiple drug resistance implies a constant need to search for novel microbial targets and to modulate their activity using artificial ...
PDF - WordPress @ Clark U
... dorsal cells (Ericson et al., 1996; Kawakami et al., 1997). Repression in the ventral region of the neural tube is one of the hallmarks of dorsal class-I transcription factors. High levels of Shh signaling also repress Pax6, although low levels of Shh are required for the induction of Pax6 (Ericson ...
... dorsal cells (Ericson et al., 1996; Kawakami et al., 1997). Repression in the ventral region of the neural tube is one of the hallmarks of dorsal class-I transcription factors. High levels of Shh signaling also repress Pax6, although low levels of Shh are required for the induction of Pax6 (Ericson ...
Oxidative Stress Contributes to Autophagy
... methyl viologen (MV), indicating that oxidative stress triggers this process in algae (Pérez-Pérez et al., 2010, 2012a). Reactive oxygen species (ROS) are potent inducers of autophagy in C. reinhardtii and plants (Liu and Bassham, 2012; Pérez-Pérez et al., 2012b). Indeed, a link between photo-oxidat ...
... methyl viologen (MV), indicating that oxidative stress triggers this process in algae (Pérez-Pérez et al., 2010, 2012a). Reactive oxygen species (ROS) are potent inducers of autophagy in C. reinhardtii and plants (Liu and Bassham, 2012; Pérez-Pérez et al., 2012b). Indeed, a link between photo-oxidat ...
LvNotch specifies secondary mesenchyme - Development
... reported that the sea urchin homologue of the LIN-12/Notch receptor, LvNotch, displays a dynamic expression pattern within both presumptive SMCs and endoderm cells during the blastula stage (Sherwood and McClay, 1997). LvNotch is expressed uniformly in cleavage-stage embryos, but becomes specificall ...
... reported that the sea urchin homologue of the LIN-12/Notch receptor, LvNotch, displays a dynamic expression pattern within both presumptive SMCs and endoderm cells during the blastula stage (Sherwood and McClay, 1997). LvNotch is expressed uniformly in cleavage-stage embryos, but becomes specificall ...
Full-Text PDF
... adjust or reverse the polarity of peptide segments or act as an intermediary between polar segments and the bilayer (Figure 4) [44]. When the methyl groups of cPHB form hydrophobic bonds to nonpolar peptide residues, its ester carbonyl oxygens form a hydrophilic surface that may associate with polar ...
... adjust or reverse the polarity of peptide segments or act as an intermediary between polar segments and the bilayer (Figure 4) [44]. When the methyl groups of cPHB form hydrophobic bonds to nonpolar peptide residues, its ester carbonyl oxygens form a hydrophilic surface that may associate with polar ...
Eh Klp5 is a divergent member of the kinesin 5 family that regulates
... We used a twofold strategy to characterize the function of Eh Klp5 – (i) to study gain of function by over-expressing this gene and (ii) to study loss of function by inhibiting expression of Eh Klp5 in E. histolytica cells. To study gain of function, Eh Klp5 was cloned under the Eh actin promoter in ...
... We used a twofold strategy to characterize the function of Eh Klp5 – (i) to study gain of function by over-expressing this gene and (ii) to study loss of function by inhibiting expression of Eh Klp5 in E. histolytica cells. To study gain of function, Eh Klp5 was cloned under the Eh actin promoter in ...
Molecule of the Month: AgrA DNA Binding Domain AgrA is the
... infection without incurring any damage. Because of its role within the Agr system, AgrA seems to be the most appealing drug target. As with all two component signaling systems in bacteria, the Agr system contains both a histidine kinase (AgrC) and a response regulator (AgrA) (Figure 1). The other co ...
... infection without incurring any damage. Because of its role within the Agr system, AgrA seems to be the most appealing drug target. As with all two component signaling systems in bacteria, the Agr system contains both a histidine kinase (AgrC) and a response regulator (AgrA) (Figure 1). The other co ...
V U Z (vzw)
... transmission may be blocked at the level of ion channels, specific receptors, G-proteins and enzymes. Interestin the action mechanism and potential therapeutic use of CoHMS toxins is only recent, but it is rapidly growing.The family of Conidae consists of more than 300 members of marine gastropods. ...
... transmission may be blocked at the level of ion channels, specific receptors, G-proteins and enzymes. Interestin the action mechanism and potential therapeutic use of CoHMS toxins is only recent, but it is rapidly growing.The family of Conidae consists of more than 300 members of marine gastropods. ...
Enzymatic lysis of microbial cells
... are functionally related to autolysins except they are phage-encoded enzymes. They digest bacterial peptidoglycan at the terminal stage of the phage reproduction cycle, allowing the release of the viral progeny out of the cell. Recent research has not only revealed the diversity of these hydrolases, ...
... are functionally related to autolysins except they are phage-encoded enzymes. They digest bacterial peptidoglycan at the terminal stage of the phage reproduction cycle, allowing the release of the viral progeny out of the cell. Recent research has not only revealed the diversity of these hydrolases, ...
Sample
... 5. What is the primary factor governing penetration of alcohols (and similar compounds) through biological membranes? Their lipid solubility. 6. Why are different concentrations of alcohols used in this experiment? To shorten the penetration times so that the experiment can be completed during the l ...
... 5. What is the primary factor governing penetration of alcohols (and similar compounds) through biological membranes? Their lipid solubility. 6. Why are different concentrations of alcohols used in this experiment? To shorten the penetration times so that the experiment can be completed during the l ...
magnetosome formation in prokaryotes
... These results indicate that magnetotaxis as a trait might have evolved several times and, moreover, could indicate that there is more than one biochemical/chemical pathway for the biomineralization of magnetic minerals by magnetotactic bacteria. Alternatively, these findings might also be explained ...
... These results indicate that magnetotaxis as a trait might have evolved several times and, moreover, could indicate that there is more than one biochemical/chemical pathway for the biomineralization of magnetic minerals by magnetotactic bacteria. Alternatively, these findings might also be explained ...
Protein thermal stability: insights from atomic displacement
... importance for industries where biocatalysts are used in extreme conditions to achieve higher solubility of substrates. It is, therefore, of primary importance to understand the factors that contribute to thermal stability. Knowledge regarding these factors has been accumulated from both experimenta ...
... importance for industries where biocatalysts are used in extreme conditions to achieve higher solubility of substrates. It is, therefore, of primary importance to understand the factors that contribute to thermal stability. Knowledge regarding these factors has been accumulated from both experimenta ...
Traffic into silence: endomembranes and
... trans-Golgi network (TGN), endosomes, and lysosomes or vacuoles. Flows of membranes and proteins to (outward) and from (inward) the plasma membrane go through these organelles. Endosomes sit at an essential position within the endomembrane system and have a core sorting function: They are the first ...
... trans-Golgi network (TGN), endosomes, and lysosomes or vacuoles. Flows of membranes and proteins to (outward) and from (inward) the plasma membrane go through these organelles. Endosomes sit at an essential position within the endomembrane system and have a core sorting function: They are the first ...
Towards Controlling the Glycoform: A Model Framework Linking
... major influence over the in vivo therapeutic mechanisms of the protein and have attracted much industrial interest. For example, with monoclonal antibodies, the largest class of biotherapeutic glycoproteins [2], the absence of the core fucose on the conserved Asn297 in the Fc region can increase ant ...
... major influence over the in vivo therapeutic mechanisms of the protein and have attracted much industrial interest. For example, with monoclonal antibodies, the largest class of biotherapeutic glycoproteins [2], the absence of the core fucose on the conserved Asn297 in the Fc region can increase ant ...
Cell division and leaf morphogenesis - Development
... al., 2000), the morphology of such plants is surprisingly normal. In addition, mutants have been identified that show altered planes of cell division throughout the plant, yet organ morphology is unaffected (Smith et al., 1996). Even mutants in which the cell division plane is severely disrupted can ...
... al., 2000), the morphology of such plants is surprisingly normal. In addition, mutants have been identified that show altered planes of cell division throughout the plant, yet organ morphology is unaffected (Smith et al., 1996). Even mutants in which the cell division plane is severely disrupted can ...
Signal transduction
Signal transduction occurs when an extracellular signaling molecule activates a specific receptor located on the cell surface or inside the cell. In turn, this receptor triggers a biochemical chain of events inside the cell, creating a response. Depending on the cell, the response alters the cell's metabolism, shape, gene expression, or ability to divide. The signal can be amplified at any step. Thus, one signaling molecule can cause many responses.