mexCD-oprJ aeruginosa
... chlorhexidine (CHX), which perturb the cell envelope. Induction of mexCD-oprJ by membrane-damaging agents requires the envelope stress sigma factor AlgU, an indication that MexCD-OprJ is a component of the envelope stress response in P. aeruginosa. In the absence of CHX-mediated envelope stress, the ...
... chlorhexidine (CHX), which perturb the cell envelope. Induction of mexCD-oprJ by membrane-damaging agents requires the envelope stress sigma factor AlgU, an indication that MexCD-OprJ is a component of the envelope stress response in P. aeruginosa. In the absence of CHX-mediated envelope stress, the ...
Checkpoints in the life-cycle of Cassiopea spp.: control of
... Experimental data reveal that most, if not all, major events in the metagenetic life. cycle of Cassiopea spp. at these checkpoints depend on the interaction with specific biotic and physical cues. For medusa formation within a permissive temperature range by monodisk strobi. lation of the polyp, the ...
... Experimental data reveal that most, if not all, major events in the metagenetic life. cycle of Cassiopea spp. at these checkpoints depend on the interaction with specific biotic and physical cues. For medusa formation within a permissive temperature range by monodisk strobi. lation of the polyp, the ...
Spatially and Functionally Distinct Roles of the PI3
... 13 DIV sympathetic neurons grown in biochemistry chambers were maintained in medium lacking NGF for 2 days before treatment with medium alone (minus sign) or medium containing NGF (150 ng/ml) for either 20 min or 8 hr. Cellular lysates were prepared from individual compartments containing cell bodie ...
... 13 DIV sympathetic neurons grown in biochemistry chambers were maintained in medium lacking NGF for 2 days before treatment with medium alone (minus sign) or medium containing NGF (150 ng/ml) for either 20 min or 8 hr. Cellular lysates were prepared from individual compartments containing cell bodie ...
Lactase persistence DNA variant enhances
... activation as compared to the C variant. Such an enhanced activity mediated by the T variant would be consistent with a functional role in the mechanism of lactase persistence. It is possible that a more dramatic effect was not seen due to the nature of transient transfection studies. Transfected Ca ...
... activation as compared to the C variant. Such an enhanced activity mediated by the T variant would be consistent with a functional role in the mechanism of lactase persistence. It is possible that a more dramatic effect was not seen due to the nature of transient transfection studies. Transfected Ca ...
Get PDF file - Botanik in Bonn
... environmental signals and requirements. The arrangement and properties of the cytoskeleton are known to be regulated by associated proteins that bind either to monomeric or polymerized cytoskeletal proteins. The number of well studied and characterized cytoskeletonregulating proteins in plant cells ...
... environmental signals and requirements. The arrangement and properties of the cytoskeleton are known to be regulated by associated proteins that bind either to monomeric or polymerized cytoskeletal proteins. The number of well studied and characterized cytoskeletonregulating proteins in plant cells ...
Review Role of a novel photopigment, melanopsin, in behavioral
... seasonal changes in day length. Chronic deficiency in the mechanism of entrainment is presumed to underlie several circadian-related disorders, such as shift-work syndrome, seasonal affective disorders and depression. Ocular photoreception serves as the major entraining stimulus for the SCN oscillat ...
... seasonal changes in day length. Chronic deficiency in the mechanism of entrainment is presumed to underlie several circadian-related disorders, such as shift-work syndrome, seasonal affective disorders and depression. Ocular photoreception serves as the major entraining stimulus for the SCN oscillat ...
Lactase persistence DNA variant enhances
... activation as compared to the C variant. Such an enhanced activity mediated by the T variant would be consistent with a functional role in the mechanism of lactase persistence. It is possible that a more dramatic effect was not seen due to the nature of transient transfection studies. Transfected Ca ...
... activation as compared to the C variant. Such an enhanced activity mediated by the T variant would be consistent with a functional role in the mechanism of lactase persistence. It is possible that a more dramatic effect was not seen due to the nature of transient transfection studies. Transfected Ca ...
Pyruvate : NADP+ Oxidoreductase from the Mitochondrion of
... type II protists, as well as the pivotal role of PFO in their anaerobic energy metabolism (Müller 1993, 1998; Fenchel and Finlay 1995; Biagini, Finlay, and Lloyd 1997), the evolutionary origin of this enzyme bears heavily on views concerning the metabolic lifestyle of the earliest eukaryotic cells ...
... type II protists, as well as the pivotal role of PFO in their anaerobic energy metabolism (Müller 1993, 1998; Fenchel and Finlay 1995; Biagini, Finlay, and Lloyd 1997), the evolutionary origin of this enzyme bears heavily on views concerning the metabolic lifestyle of the earliest eukaryotic cells ...
Induction of MicroRNA-1 by Myocardin in Smooth Muscle Cells
... myocardin-inducible SMCs, but not in control cells with empty vectors (Figure 1B and data not shown). Of note, after 3 days of Dox induction, the miR-1 level in cultured SMCs became comparable to that in intact carotid artery (Figure 1B). Subsequently, we used Northern blot assay to confirm the expr ...
... myocardin-inducible SMCs, but not in control cells with empty vectors (Figure 1B and data not shown). Of note, after 3 days of Dox induction, the miR-1 level in cultured SMCs became comparable to that in intact carotid artery (Figure 1B). Subsequently, we used Northern blot assay to confirm the expr ...
Universitat Autònoma de Barcelona
... Naunyn Schmiedeberg’s Archives of Pharmacology, Oct 2011;384(4-5):373-381 3. Bacterial inclusion bodies of Alzheimer’s disease β-amyloid peptides can be employed to study native-like aggregation intermediate states Dasari M, Espargaró A, Sabaté R, Lopez del Amo JM, Fink U, Grelle G, Bieschke J, Vent ...
... Naunyn Schmiedeberg’s Archives of Pharmacology, Oct 2011;384(4-5):373-381 3. Bacterial inclusion bodies of Alzheimer’s disease β-amyloid peptides can be employed to study native-like aggregation intermediate states Dasari M, Espargaró A, Sabaté R, Lopez del Amo JM, Fink U, Grelle G, Bieschke J, Vent ...
Pyruvate : NADP+ Oxidoreductase from the Mitochondrion of
... type II protists, as well as the pivotal role of PFO in their anaerobic energy metabolism (Müller 1993, 1998; Fenchel and Finlay 1995; Biagini, Finlay, and Lloyd 1997), the evolutionary origin of this enzyme bears heavily on views concerning the metabolic lifestyle of the earliest eukaryotic cells ...
... type II protists, as well as the pivotal role of PFO in their anaerobic energy metabolism (Müller 1993, 1998; Fenchel and Finlay 1995; Biagini, Finlay, and Lloyd 1997), the evolutionary origin of this enzyme bears heavily on views concerning the metabolic lifestyle of the earliest eukaryotic cells ...
PDF
... MDR1 gene, which functions as an ATP-dependent drug efflux pump, is N-linked glycosylated at asparagine residues 91, 94, and 99 located within the first extracellular loop. We report here the biochemical characterization of glycosylation-deficient (Gly−) P-gp using a vaccinia virus based transient e ...
... MDR1 gene, which functions as an ATP-dependent drug efflux pump, is N-linked glycosylated at asparagine residues 91, 94, and 99 located within the first extracellular loop. We report here the biochemical characterization of glycosylation-deficient (Gly−) P-gp using a vaccinia virus based transient e ...
The semi-phosphorylative Entner–Doudoroff pathway in
... 103 M−1 · cm−1 ) [29,30]. Galactonate (10 mM) was prepared from galactonate γ -lactone by incubation in 1 M NaOH for 1 h (4 M stock solution) and subsequent dilution in 50 mM Hepes/KOH (pH 7.0, 70 ◦C) as described in [31]. The activity of the T. tenax and S. solfataricus KDG kinases was determined a ...
... 103 M−1 · cm−1 ) [29,30]. Galactonate (10 mM) was prepared from galactonate γ -lactone by incubation in 1 M NaOH for 1 h (4 M stock solution) and subsequent dilution in 50 mM Hepes/KOH (pH 7.0, 70 ◦C) as described in [31]. The activity of the T. tenax and S. solfataricus KDG kinases was determined a ...
KATP channels and preconditioning - Philipps
... central mediator of preconditioning [34,50–53]. Liu et al. [54] even proposed that activation of PKC, which requires its translocation from the cytosol to anchoring proteins on the membrane cytoskeleton [55], produces the preconditioned state. This idea was supported by experiments showing that PKC ...
... central mediator of preconditioning [34,50–53]. Liu et al. [54] even proposed that activation of PKC, which requires its translocation from the cytosol to anchoring proteins on the membrane cytoskeleton [55], produces the preconditioned state. This idea was supported by experiments showing that PKC ...
Anatomy and Physiology of Smell
... interact with mucus overlying the receptor cells Produced by Bowman’s glands and adjacent respiratory mucosa (goblet cells) Partitioning of odorant’s molecules between air phase and mucus phase important in reaching olfactory epithelium Must be soluble in mucus but not too strongly captured to inter ...
... interact with mucus overlying the receptor cells Produced by Bowman’s glands and adjacent respiratory mucosa (goblet cells) Partitioning of odorant’s molecules between air phase and mucus phase important in reaching olfactory epithelium Must be soluble in mucus but not too strongly captured to inter ...
![[ H]GABA and - The University of Sydney](http://s1.studyres.com/store/data/016554642_1-7f0b2cb4433d1c3e90388924056f48a4-300x300.png)
[ H]GABA and - The University of Sydney
... free and cut (first in a sagittal direction and then in a transverse direction) using a McIlwain tissue chopper (three cerebella in 33 ml of KrebsRinger-HEPES buffer, pH 7.4; 0.1 x 0.1 µm). Smaller slices were used for the uptake experiments than for the release experiments. The smaller slices incre ...
... free and cut (first in a sagittal direction and then in a transverse direction) using a McIlwain tissue chopper (three cerebella in 33 ml of KrebsRinger-HEPES buffer, pH 7.4; 0.1 x 0.1 µm). Smaller slices were used for the uptake experiments than for the release experiments. The smaller slices incre ...
Structure-Function Analysis of SH3 Domains: SH3
... family protein tyrosine kinases (13, 14). This domain mediates protein-protein interactions that are important for coupling of intracellular signaling pathways, regulation of catalytic activity of proteins, recruitment of substrates to enzymes, and localization of proteins to a specific subcellular ...
... family protein tyrosine kinases (13, 14). This domain mediates protein-protein interactions that are important for coupling of intracellular signaling pathways, regulation of catalytic activity of proteins, recruitment of substrates to enzymes, and localization of proteins to a specific subcellular ...
Solution Structure of the Tandem Acyl Carrier Protein Domains from
... (ACP) domains in tandem. This conserved tandem arrangement has been implicated in both amplification of fatty acid production (additive effect) and in structural stabilization of the multidomain protein (synergistic effect). While the more accepted model is one in which domains act independently, re ...
... (ACP) domains in tandem. This conserved tandem arrangement has been implicated in both amplification of fatty acid production (additive effect) and in structural stabilization of the multidomain protein (synergistic effect). While the more accepted model is one in which domains act independently, re ...
E. Coli MurG: A Paradigm for a Superfamily of
... Crystals of E. coli MurG containing a C-terminal LEHHHHHH sequence were grown at room temperature using the hanging-drop vapor diffusion method in a NaMES buffer (pH 6.5) containing 0.5 M (NH4)2SO4 and 0.2% Triton X-100. Triclinic crystals belonging to the P1 space group and having two molecules per ...
... Crystals of E. coli MurG containing a C-terminal LEHHHHHH sequence were grown at room temperature using the hanging-drop vapor diffusion method in a NaMES buffer (pH 6.5) containing 0.5 M (NH4)2SO4 and 0.2% Triton X-100. Triclinic crystals belonging to the P1 space group and having two molecules per ...
Cardiac Ionic Currents and Acute Ischemia
... electrons to O2 through the electron transport chain. This provides the energy to build up the chemiosmotic gradient that drives the synthesis of ATP. Oxidative phosphorylation is coupled to the demands of the cell. A feedback is generated by the breakdown products of ATP (772) and the rise in mitoc ...
... electrons to O2 through the electron transport chain. This provides the energy to build up the chemiosmotic gradient that drives the synthesis of ATP. Oxidative phosphorylation is coupled to the demands of the cell. A feedback is generated by the breakdown products of ATP (772) and the rise in mitoc ...
Identification of core functional region of murine IL-4 using
... analysis of peptide phage display. (A) The affinity of 11B.11 binding to IL-4 or phage-displayed peptide was detected by competition ELISA. 11B.11 was used to coat a multi-well plate, C6 clone in three dilution (106 for diamonds, 107 for squares and 108 for triangles) and control phage clone of f1 ( ...
... analysis of peptide phage display. (A) The affinity of 11B.11 binding to IL-4 or phage-displayed peptide was detected by competition ELISA. 11B.11 was used to coat a multi-well plate, C6 clone in three dilution (106 for diamonds, 107 for squares and 108 for triangles) and control phage clone of f1 ( ...
PapD-like chaperones and pilus biogenesis
... The crystal structure of PapD and the solution structure of FimC, and more recently, the crystal structures of the FimC᎐FimH chaperone᎐adhesin complex and the PapD᎐PapK chaperone᎐pilin complex have all been solved.25 ᎐ 28 The chaperone consists of two immunoglobulin-like ŽIg. domains oriented in an ...
... The crystal structure of PapD and the solution structure of FimC, and more recently, the crystal structures of the FimC᎐FimH chaperone᎐adhesin complex and the PapD᎐PapK chaperone᎐pilin complex have all been solved.25 ᎐ 28 The chaperone consists of two immunoglobulin-like ŽIg. domains oriented in an ...
Identification of genes specifically required for the
... hydrocarbon constituents in petroleum reservoirs have been elucidated, the mechanisms for anaerobic activation of benzene, a very stable molecule, are not known. Previous studies have demonstrated that Geobacter metallireducens can anaerobically oxidize benzene to carbon dioxide with Fe(III) as the ...
... hydrocarbon constituents in petroleum reservoirs have been elucidated, the mechanisms for anaerobic activation of benzene, a very stable molecule, are not known. Previous studies have demonstrated that Geobacter metallireducens can anaerobically oxidize benzene to carbon dioxide with Fe(III) as the ...
Direct redox modulation of p53 protein: potential sources of redox
... the cell. The three common types of intracellular ROI postulated to be important for modulating protein redox levels are hydrogen peroxide, hydroxyl radicals and superoxide. Specific enzymes have evolved to rid the cell of hydrogen peroxide and superoxide and these enzymes are, in fact, regulated in ...
... the cell. The three common types of intracellular ROI postulated to be important for modulating protein redox levels are hydrogen peroxide, hydroxyl radicals and superoxide. Specific enzymes have evolved to rid the cell of hydrogen peroxide and superoxide and these enzymes are, in fact, regulated in ...
Signal transduction
Signal transduction occurs when an extracellular signaling molecule activates a specific receptor located on the cell surface or inside the cell. In turn, this receptor triggers a biochemical chain of events inside the cell, creating a response. Depending on the cell, the response alters the cell's metabolism, shape, gene expression, or ability to divide. The signal can be amplified at any step. Thus, one signaling molecule can cause many responses.